Mast3 (NM_001134796) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205714 representing NM_001134796 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGACCCCAGCTACTGGACCGCGGTGGCGGCCCCCGGACACCGCAGCCGCCTGGCCAAGGGTGCGC TGCTACAACGTTCCAAAAGCTGCCGCAGTGGCAACCGTAAGAGCCTAGTCGTGGGGACGCCTTCACCCAC TCTCTCCAGACCTTTGTCACCACTCTCTGTCCCAACTGCAGGTAACAGCCCCTTGGACAGTCCTCGGAAC TTCTCTGCCGCAGCGGCCATCAATTTCCCCTTTGCCCGGAGGGCTGACGGCAGAAGATGGTCACTTGCTT CCCTCCCGTCATCAGGCTATGGGACCAACACTCCCAGCTCCACTGTGTCGTCAAGCTCATCCTCCCGGGA GCGTCTACACCAGCTCCCTTTCCAACCCACTGCGGACGAACTGCGCTTCCTGTCCAAGCACTTCCGCAGC TCAGAGAGTGTGGTGGATGAGGATGGAGGCCGTTCTCCGCGCCTGCGCCCACGCTCACGAAGCCTCAGCC CTGGACGAACATCAGGAACCTTCGACAACGAGATAGTGATGATGAATCATGTATACCGGGAGCGCTTCCC CAAGGCCACAGCGCAGATGGAGGGCCGGCTGCAGGACTTCCTGGCAGCATTTGCTCCTGGAGACCGCCTG GCACTGGCAGACGGTGTCCTGGGCTTCATCCACCACCAGATCGTGGAGTTGGCGAGGGACTGCCTGGCCA AGTCGGGCGAGGCTCTAGTCACCTCCCGCTATTTCCTGGAGATGCAAGACAAGCTGGAGCGACTACTGCA GGATGCTCATGAGAGGTCAGATAGCGCCGAAGTGGGCTTCATCGTGCAGTTGGTCCGCAAGCTGCTCATC ATCATCTCCAGGCCAGCGCGGCTGCTCGAGTGCTTGGAGTTCGACCCCGAAGAATTCTACCATCTGCTGG AGGCTGCAGAGGGCCAGGCCCGCGAGGACCAGGGCGTGAAGACTGATCTGCCCCGCTACATCATCCGCCA GCTGGGCCTTGCCAAGGACCCTCTAGAAGAGATTCAGCCCCTGAATGACCTTGATGAAGGCCAGCCACCA GCTCCTGGATCCCCTGAGAGCCGAGGCCTGGGCGGTCCGTCTCGGAGGCAGCCATGTGAGAGCGACTTTG AGACCATCAAGCTTATTAGCAATGGAGCCTATGGGGCTGTCTACCTGGTGCGCCACAGGGACACACGACA GCGCTTCGCCATCAAGAAGATCAACAAGCAGAATTTGATTCTGCGCAACCAGATCCAACAGGTGTTTGTG GAGCGAGACATCCTCACCTTCGCCGAGAACCCCTTTGTGGTCGGCATGTTCTGCTCTTTTGAAACCCGTC GCCACCTCTGCATGGTGATGGAGTATGTGGAAGGGGGTGACTGCGCCACGCTGCTGAAAAACATGGGCCC ACTGCCTGTGGACATGGCCCGCATGTACTTCGCGGAGACGGTACTGGCTCTGGAGTATCTGCACAACTAT GGCATCGTGCACCGAGACCTCAAACCTGACAACCTGCTCATCACCTCCCTCGGCCACATCAAGCTGACGG ACTTCGGCCTGTCCAAGATCGGCCTCATGAGCATGGCCACCAACCTGTATGAAGGTCACATCGAGAAGGA TGCTCGAGAGTTTGTGGACAAGCAGGTGTGCGGGACGCCTGAATACATCGCGCCAGAGGTGATCTTCCGC CAGGGCTATGGGAAGCCAGTAGACTGGTGGGCCATGGGCGTCATCCTGTATGAGTTCCTGGTGGGCTGCG TGCCTTTCTTTGGGGACACACCTGAGGAGCTGTTCGGACAGGTTGTCAGTGATGAGATCATGTGGCCAGA GGGAGATGAGGCCCTTCCCCCCGACGCCCAGGATCTCATTACTAGGCTTCTGCGTCAGAGCCCAATGGAC AGGCTGGGGACTGGAGGCACCCACGAGGTGAAACAGCACCCCTTCTTCCTGGCCCTGGACTGGGCAGGGC TCCTGAGACACAAGGCAGAGTTTGTCCCACAGCTTGAAGCTGAGGACGACACCAGCTACTTTGACACTCG CTCTGAGCGGTACCGCCACTTGGGCTCCGAGGGTGACGAGACGAATGACGAGGAATCCTCCACAGAGATC CCCCAGTTCTCTTCCTGTTCCCACCGCTTCAGCAAGGTCTACAGCAGTTCCGAGTTCCTGGCTGTCCAGC CCAACCCCACCTTTGCTGAAAGAAGCTTCAGTGAGGACCGAGAGGAAGGTTGGGAGCAAAGCGGTGAAGG GGACGGTGGCCGCCGGCTGAGCACTGACCTCCGGCTGAGGTCCTGGACACCTGCTTCCTCCTGTCAGCCG TCTTCCTGTCAGAGCGACCGGGGTCCCAGCCCGTCTCTCCTAAGCACCATCAGCTTGGACATGCCTAAAT TTGCCTTCTCCTCTGAAGATGAGGGAGCAGGCTCAGGGCCTGCTGACACCAGAAAGCCCGTCTTCATCTT GGGGGAGCCTGACCCCCCACCCCCGACCACCCCAGTGACACCTAAGCCCTGCAACCTTTCTGCCGACACA GCTGTTCTCAGCCATGCCCGCCTTCGAAGTAACAGTACAGGCGCCCGGCACTCAACTCCTCGGCCCCTGG ATGCCGGCAGAGGCCGCCGCCTAGGAAGCTCAAGAGACCCCGGCCCAGAGAAGCCCAGGGCCTCCCCTGG TGGCAGCGGGGGTCGAGTGCCCAAGTCAGCCTCTGTGTCTGCCCTGTCCCTCATCATCACGGCAGACGAC GGCAGTGGGGGCCCCCTCATGAGTCCCTTGTCGCCCCGCTCGCTGTCCTCCAACCCCTCCTCCCGAGACT CCTCACCTAGCCGGGACCCATCACCAGTGTGTGGTAGCCTGCGGCCCCCCATCGTCATCCACAGCTCGGG CAAGAAGTACGGCTTCAGCCTTCGCGCCATTCGTGTCTACATGGGAGACAGCGACGTGTACACGGTGCAC CATGTGGTCTGGAGCGTGGAGGAGGGGAGCCCTGCGCAGGAGGCGGGACTGCGTGCTGGGGACCTCATCA CACACATCAACGGGGAATCTGTCCTGGGCCTGGTGCACATGGATGTGGTGGAGCTGCTGCTGAAGAGTGG CAACAAGATCTCCCTGCGCACCACGGCCCTGGAGAACACCTCCATCAAGGTGGGGCCTGCCCGGAAGAAC GTGGCCAAGGGCCGCATGGCCCGAAGAAGCAAGCGCAGCCGCCGGCGCGAGACCCAGGACCGGCGGAAGT CCTTGTTTAAGAGGATCTCGAAGCCATCTTCTGTGCTCCACACCAGCCGTAGTTTCTCCTCGGGCCTCCA GCACTCTCTGTCCTCCAGCGAGAGCCTCCCTGGATCACCCACTCACAGCCTGTCCCCTAGCCCCACCACG CCCTGCCGCAGCCCTGCCCCCGACGTCCCCACAGACACAGCATCCCCACCCAGTGTATCCCCAAGTTCCA GCAGTCCCGCTTCTCCAGCAACTGGCCACACCCGTCCCAGCTCACTTCATGGCCTGGCAGCCAAGCTCGG GCCACCTCGACACAAGAGCGGCCGCCGCAAGTCCACAAGCAGTATCCCTCCGTCACCACTGGCTTGCCCG CCAGTGCCCACACCCCCACCACGCTCGCCGTCTCCCCTTCCTGGGCACATTCCCATACCAGCACGGTCCC CTAGGCTGCGTAGGGGCCAGTCTGCCGATAAGCTGGGCCTAGGCACCAGTGAGAGGCTGGATGGGGACGG AGGCCGGCGTGGCAGAGGGGCCGAGACTGAGCTGGTCGTCATGAGGAGGCTGCATCTGTCAGAGCGCCGG GACTCCTTCAAGAAGCAGGAGGCCGTGCAGGAAGTGAGCTTCGATGAGGAGCCTGGGCCGACTGGTGCGG TGCCGAAGATCGCTGTGCAGGGTGCAGAGGCCACGCCTGGAAGCCCAGGACCAGCCAGGGAAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001134796
Insert Size	3918 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001134796.1, NP_001128268.1
RefSeq Size	5371 bp
RefSeq ORF	3918 bp
Locus ID	688540

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