United States
Germany
Japan
United Kingdom
China
Aplnr (NM_031349) Rat Untagged Clone
CAT＃: RN205740
Aplnr (untagged ORF) - Rat apelin receptor (Aplnr), (10 ug)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Agtrl1; Apj |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN205740 representing NM_031349
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGATGATGGTTACAACTACTACGGGGCTGACAACCAGTCTGAATGTGACTACGCAGACTGGACGC CCTCTGGAGCTCTCATCCCTGCCATCTACATTCTGGTTTTCCTTCTAGGCACCACAGGCAATGGCCTGGT GCTCTGGACCGTGTTTTGGAGCAGCCGAGAAAAGAGACGCTCAGCTGACATCTTCATTGCCAGCCTGGCT GTGGCTGACTTGACCTTTGTGGTGACTTTGCCACTGTGGGCCACTTATACCTACCGGGAGTTTGACTGGC CTTTTGGAACCTTCTCTTGCAAGCTCAGCAGCTACCTCATCTTTGTCAACATGTACGCCAGTGTCTTTTG CCTCACCGGCCTCAGCTTTGACCGATACCTGGCCATTGTCAGGCCAGTGGCTAACGCTCGACTAAGGCTG CGAGTCAGCGGGGCCGTGGCCACAGCAGTCTTGTGGGTGCTGGCTGCCCTTCTAGCTGTGCCCGTCATGG TGTTCCGTTCCACTGACATCCCGGAAAACAGCACCAAGACCCAGTGCTACATGGACTACTCTATGGTGGC CACTTCAAACTCAGAGTGGGCCTGGGAGGTGGGCCTTGGGGTGTCCTCCACTGCTGTGGGCTTTGTGGTG CCCTTCATCATCATGCTGACCTGTTACTTCTTCATTGCCCAAACCATCGCTGGCCATTTCCGAAAGGAGC GCATCGAGGGCCTGCGGAAGAGGCGCCGGCTGCTGAGCATCATCGTGGTGCTGGTGGTGACCTTTGCCCT GTGCTGGATGCCTTACCACCTGGTGAAGACTCTCTACATGCTGGGCAATTTGCTGCACTGGCCCTGTGAC TTTGACAGCTTCCTCATGAATGTCTTTCCCTATTGCACCTGCATCAGTTACGTCAACAGCTGCCTCAACC CCTTCCTCTATGCCTTCTTTGACCCCCGATTTCGCCGAGCCTGCACCTCCATGCTCTGCTGTGACCAGAG CGGGTGCAAAGGCAGCCCTCACAGCAGCAGTGCAGAGAAATCTGCCAGTTATTCTTCTGGGCACAGCCAG GGCCCCGGCCCCAACATGTGCAAGGGAGGAGAACCGATGCATGAGAAATCTATCCCCTATAGCCAAGAAA CCCTTGTGGACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_031349 |
| Insert Size | 1134 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031349.2, NP_112639.1 |
| RefSeq Size | 3670 bp |
| RefSeq ORF | 1134 bp |
| Locus ID | 83518 |
| UniProt ID | Q9JHG3 |
| Gene Summary | Receptor for apelin receptor early endogenous ligand (APELA) and apelin (APLN) hormones coupled to G proteins that inhibit adenylate cyclase activity (PubMed:11359874). Plays a key role in early development such as gastrulation, blood vessels formation and heart morphogenesis by acting as a receptor for APELA hormone. May promote angioblast migration toward the embryonic midline, i.e. the position of the future vessel formation, during vasculogenesis. Promotes sinus venosus (SV)-derived endothelial cells migration into the developing heart to promote coronary blood vessel development. Plays also a role in various processes in adults such as regulation of blood vessel formation, blood pressure, heart contractility and heart failure (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR205740 | Aplnr (Myc-DDK-tagged ORF) - Rat apelin receptor (Aplnr), (10 ug) |
CNY 5,488.00 |
|
| RR205740L3 | Lenti ORF clone of Aplnr (Myc-DDK-tagged ORF) - Rat apelin receptor (Aplnr), (10 ug) |
CNY 6,080.00 |
|
| RR205740L4 | Lenti ORF clone of Aplnr (mGFP-tagged ORF) - Rat apelin receptor (Aplnr), (10 ug) |
CNY 6,650.00 |
