Ptprj (NM_017269) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	DEP-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205764 representing NM_017269 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCCGGGGAAGCCCGGGGCGGGCGGAGCGGGGACTAGGCGGACCGGCTGGCGGAGAAGGAGGCGGA GGCGTCGGCTGGAGGCGGAGACCAGGGCGCCCGGCTTCGGGCACACGGCGGGGCGCGTCCCGGGCACGTT CCAGGGCGCGCAGGGCATGAAGCCCGCGGCGCGGGAGACGCGGACACCCCCGCGCTCGCCCGGGCTCCGC TGGGCGCTGCTGCCGCTGCTGCTGTTGCTACGCCAGGGCCAGGTTGTGTGCGCAGGTGCGGCGCCCAGTC CTGTATTTGACGTTGAAGCTGTCACCAGCCCTACCAGTGTGGTATTAACGTGGAAACACAATGATTCAGC CACTTCAGAATATAAGATAAATGAGGGAAACACGCTGCGGTATACTGTTAAAAACCAGACATCATTTAAC ATCACAGGCTTAAGCCCAGCTACTTCCTACAAATTCTCCATCACCTTGGGAACAGTCAATGAGACCTCAG GAAAGCCCACATACAAAAATATCACAACAGAGCCCTGGCCAGTGTCGGATCTCCAAGTCGCCTACATCGG TGTGACACAGGCTCTTCTCGCCTGGAGCAATGCAAACGGCACCGCTTCTTACCGGATGCAGATTGTAGAG CTGACCACAAATTCATCAGGTGGTATTTCAGATCTGAAGCCGGGGACCCATAAGAGCTTGGCTGTCCAAG GATCGAATGAGACCCAGCATGACTTATGGGTCACAGAAGGCGTCTCGGATGCCAATGGCACCGAGGGAAG CCCAGTGGCCAACATATCCCAACTACATAAGAATTCTCTTGCCTCTGCAGACCCACCCTCTGCCCGGGAT CCCTCCCTCACAGAGGTCTTGCTCACTGAGCTAAAGCCTGATACTCAGTACAAAGTCACCATTTATTCTC AAGCAGCAGATGGCACAGAAGGACAGCCTGGGAACAAAGTGTTTAAAACAAATCCCATCCAAGTTTCTGA CATCCGAGCTGTGAACATCAGTGACTCGAACATGACCCTGACCTGGAAAAGCAACAATAATGAGTCCCAT GCTTCATTTACCTACAAAATATACGTGGCTGGGGGTTCCGACTCCATCAACGAAACTGTCAATGAGACTC AGGCCGTCATCCGTGGACTCAGCTCCAGTACCTTGTACAACATCACAGTGCTTCCTTTCCTGGGTCAGAC CGCGGGTATACCAGGCTTCCTCCAAGTGTACACTTCCCCCCGTCCTGTTTCTGACTTCCGAGTGACAAAT GTCAGCCTGAGGGAAATCGGTTTGGCTTGGAGAAGCAATGACTCGGAGTCATTCGAGATTTTCATCACGC AGGAGGGAAGTGAGAAACGTTGGAATGCTTCGACGGGAGACCTGAGCTATATCGTTGAAAATTTAAAGCC TGGGACCAGTTACCAATTTGAAATATTCCCACGAGGACCAAATGGGACTGAAGGGCCATCCCAGACAGTT GTTGGTAGAACTGACTGCAGTGCTGTGACTGACATCCGCGTGGTCAGCGTTAGCACCACTGAAATACAGC TGGAGTGGCAGAATACAGACAGTGCTTCTGGATACACTTACCATTTAGTTCTAGAGTCTGAGAATGGCTC CATCAAGACCAACAGTTCTCAGAAATGGATCACAGTGGGGGGCCTTACCCCAGGCACCTTATACAATGTT ACAATCTTTCCAGAAGTGGACCAGATGGAGGGCAACTCCAGCTCTATTACCCAGTACACAAGGCCCAGCA ATGTGTCCTACATTGAAGTAAACACCAACACCACCGTGGCAGCCATCCAGTGGAAGAACCTGGACGCAGC CTCTGCTTCCTACTCCTACTCCGTTCTTATCTTGAAGGCTGGAGATGGCAGCAATGTAACCAGCAGGGTC AGAGACATCCCTTCTGTCACCATCCCTGGGCTAATCCCCGGCGTCTCTTACGAAGTGAAGATCTTTACAA AGATTAGGAATACAGAGGTTGGGAATGAGGTACCTGGTCAGAAGCTGTTCTGTATGGAACCTGCACAAGT GGATTCCTTACACTGTGAAGTGGTCCCGAAGGAGCCAGCGCTGGTTCTGAAGTGGGCCTGCCCCCCTGGC ATGAACTCAGGCTTTGAACTGGGGGTCAGGAGTGATGCTTGGGACAATATGACACACTTGGAGAACTGCA CTTTGGACAATGACACAGAGTGCAGGACAGAAGTCACCTATTTGAATTTTTCTACCTCGTACAACATCAG CATTGCCACCTTGTCATGTGGAAAGATGGCATTGCCCACCCAGAGCACCTGCACCACTGGCATCACAGAC CCACCTCCTCCAGATGGATCCCCTAATATTACATCGGTCAGTCACAATTCAGTAAAGGTGAAGTTCAGTG GTTTTGAAGCCAGCCATGGACCTATCAAAGCCTATGCTGTCCTTCTCACCACAGGGGAAGCTGGCCAACC CTCCACAGATGTTTTGAAGTACACATATGAAGATTTCAAAAAGGGAGCCTCCGATACTTATGTGACATAC CTCATAAGGATAGAAGAGAAGGGACAGTCTCAGGGCTTGTCTGAAGCATTGAACTATGAGATTGATGTGG GGAACCAATCCACTACCCTCGGCTACTACAACGGGAGGCTAGAGCCTCTGGGCTCCTACCGGGCTTGCGT TGCTGGCTTTACCAATATTACCTACAACCTTCAGAACGATGGCCTTATTAATGGGGATGAGAGCTATGTA TCTTTCAGTCCGTATTCAGAGGCCGTGTCCTTGCCTCAAGATCCAGGTGTCATCTGCGGAGCGGTGTTCG GATGTATCTTTGGTGCCCTGGCCATTGTGGCTGTGGGAGGCTTCATCTTCTGGAGAAAGAAAAGGAAAGA TGCCAAGAATAATGAAGTGTCCTTTTCTCAAATTAAATCCAAGTTAATCCGAGTGGAGAATTTTGAGGCC TACTTTAAGAAACAGCAAGCCGACTCCAACTGTGGGTTTGCAGAGGAATATGAGGATCTGAAGCTGATTG GAATAAGTTTACCTAAATATGCAGCGGAAATAGCTGAAAACAGGGGGAAGAACCGCTACAACAACGTCCT GCCCTATGATATTTCTCGAGTCAAACTTTCAGTCCAGACCCATTCGACAGACGACTACATCAATGCCAAC TATATGCCTGGCTACCATTCCAAGAAAGATTTCATTGCCACACAAGGACCTTTACCCAACACTTTGAAAG ATTTTTGGCGTATGGTTTGGGAGAAAAACGTATATGCCATTGTTATGCTGACCAAATGTGTGGAGCAGGG AAGGACCAAATGTGAAGAGTACTGGCCTTCCAAGCAGGCTCAGGACTACGGGGACATAACTGTGGCAATG ACATCAGAAGTTGTTCTTCCGGAATGGACCATCAGAGATTTTGTGGTGAAAAATATGCAGAGTAGTGAGA GTCATCCTCTGCGGCAGTTCCATTTCACCTCCTGGCCTGACCATGGTGTTCCTGACACCACCGACCTGCT CATCAACTTTCGGTACCTGGTCCGGGATTACATGAAGCAGATCCCCCCTGAGTCACCAATCCTGGTGCAT TGCAGTGCTGGGGTTGGAAGGACGGGCACTTTCATTGCTATTGATCGCCTGATCTATCAGATAGAGAACG AGAACACCGTGGATGTGTATGGCATTGTCTACGATCTTCGGATGCACAGGCCTCTGATGGTACAGACAGA GGACCAGTATGTTTTCCTCAATCAGTGTGTGTTGGATATTATCAGAGCCCAGAAAGACTCAAAAGTTGAC CTCATCTATCAGAACACAACCGCAATGACAATCTATGAAAACCTCGAGCCAGTGAGCATGTTTGGAAAGA CTAATGGTTACATCGCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_017269
Insert Size	3870 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017269.2, NP_058965.2
RefSeq Size	7500 bp
RefSeq ORF	3870 bp
Locus ID	29645
Gene Summary	protein tyrosine phosphatase expressed in smooth muscle cells; may be involved in cell adhesion or cell-cell signaling [RGD, Feb 2006]

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