Erbb2 (NM_017003) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN205833 representing NM_017003 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCATCATGGAGCTGGCGGCCTGGTGCCGCTGGGGGTTCCTCCTCGCCCTCCTGCCCCCCGGAATCG CGGGCACCCAAGTGTGTACCGGCACAGACATGAAGTTGCGGCTCCCTGCCAGTCCTGAGACCCACCTGGA CATGCTCCGCCACCTGTACCAGGGCTGTCAGGTAGTGCAGGGCAACTTGGAGCTTACCTACGTGCCTGCC AATGCCAGCCTCTCATTCCTGCAGGACATCCAGGAAGTTCAGGGTTACATGCTCATCGCTCACAACCAGG TGAAGCGCGTCCCACTGCAAAGGCTGCGCATCGTGAGAGGGACCCAGCTCTTTGAGGACAAGTATGCCCT GGCTGTGCTAGACAACCGAGATCCTCAGGACAATGTCGCCGCCTCCACCCCAGGCAGAACCCCAGAGGGG CTGCGGGAGCTGCAGCTTCGAAGTCTCACAGAGATCCTGAAGGGAGGAGTTTTGATCCGTGGGAACCCTC AGCTCTGCTACCAGGACATGGTTTTGTGGAAGGACGTCTTCCGCAAGAATAACCAACTGGCTCCTGTCGA TATAGACACCAATCGTTCCCGGGCCTGTCCACCTTGTGCCCCCGCCTGCAAAGACAATCACTGTTGGGGT GAGAGTCCGGAAGACTGTCAGATCTTGACTGGCACCATCTGTACCAGTGGTTGTGCCCGGTGCAAGGGCC GGCTGCCCACTGACTGCTGCCATGAGCAGTGTGCCGCAGGCTGCACGGGCCCCAAGCATTCTGACTGCCT GGCCTGCCTCCACTTCAATCATAGTGGTATCTGTGAGCTGCACTGCCCAGCCCTCGTCACCTACAACACA GACACCTTTGAGTCCATGCACAACCCTGAGGGTCGCTACACCTTTGGTGCCAGCTGCGTGACCACCTGCC CCTACAACTACCTGTCTACGGAAGTGGGATCCTGCACTCTGGTGTGTCCCCCGAATAACCAAGAGGTCAC AGCTGAGGACGGAACACAGCGTTGTGAGAAATGCAGCAAGCCCTGTGCTCGAGTGTGCTATGGTCTGGGC ATGGAGCACCTTCGAGGGGCGAGGGCCATCACCAGTGACAATGTCCAGGAGTTTGATGGCTGCAAGAAGA TCTTTGGGAGCCTGGCATTTTTGCCGGAGAGCTTTGATGGGGACCCCTCCTCCGGCATTGCTCCGCTGAG GCCTGAGCAGCTCCAAGTGTTCGAAACCCTGGAGGAGATCACAGGTTACCTGTACATCTCAGCATGGCCA GACAGTCTCCGTGACCTCAGTGTCTTCCAGAACCTTCGAATCATTCGGGGACGGATTCTCCACGATGGCG CGTACTCATTGACACTGCAAGGCCTGGGGATCCACTCGCTGGGGCTGCGCTCACTGCGGGAGCTGGGCAG TGGATTGGCTCTGATTCACCGCAACGCCCATCTCTGCTTTGTACACACTGTACCTTGGGACCAGCTCTTC CGGAACCCACATCAGGCCCTGCTCCACAGTGGGAACCGGCCGGAAGAGGATTGTGGTCTCGAGGGCTTGG TCTGTAACTCACTGTGTGCCCACGGGCACTGCTGGGGGCCAGGGCCCACCCAGTGTGTCAACTGCAGTCA TTTCCTTCGGGGCCAGGAGTGTGTGGAGGAGTGCCGAGTATGGAAGGGGCTCCCCCGGGAGTATGTGAGT GACAAGCGCTGTCTGCCGTGTCACCCCGAGTGTCAGCCTCAAAACAGCTCAGAGACCTGCTTTGGATCGG AGGCTGATCAGTGTGCAGCCTGCGCCCACTACAAGGACTCGTCCTCCTGTGTGGCTCGCTGCCCCAGTGG TGTGAAACCGGACCTCTCCTACATGCCCATCTGGAAGTACCCGGATGAGGAGGGCATATGCCAGCCGTGC CCCATCAACTGCACCCACTCCTGTGTGGATCTGGATGAACGAGGCTGCCCAGCAGAGCAGAGAGCCAGCC CGGTGACATTCATCATTGCAACTGTAGTGGGCGTCCTGCTGTTCCTGATCTTAGTGGTGGTCGTTGGAAT CCTAATCAAACGAAGGAGACAGAAGATCCGGAAGTATACGATGCGTAGGCTGCTGCAGGAAACTGAGTTA GTGGAGCCGCTGACGCCCAGCGGAGCAATGCCCAACCAGGCTCAGATGCGGATCCTAAAAGAGACGGAGC TAAGGAAGGTGAAGGTGCTTGGATCAGGAGCTTTTGGCACTGTCTACAAGGGCATCTGGATCCCAGATGG GGAGAATGTGAAAATCCCCGTGGCTATCAAGGTGTTGAGAGAAAACACATCTCCTAAAGCCAACAAAGAA ATTCTAGATGAAGCGTATGTGATGGCTGGTGTGGGTTCTCCGTATGTGTCCCGCCTCCTGGGCATCTGCC TGACATCCACAGTACAGCTGGTGACACAGCTTATGCCCTACGGCTGCCTTCTGGACCATGTCCGAGAACA CCGAGGTCGCCTAGGCTCCCAGGACCTGCTCAACTGGTGTGTTCAGATTGCCAAGGGGATGAGCTACCTG GAGGACGTGCGGCTTGTACACAGGGACCTGGCTGCCCGGAATGTGCTAGTCAAGAGTCCCAACCACGTCA AGATTACAGATTTCGGGCTGGCTCGGCTGCTGGACATTGATGAGACAGAGTACCATGCAGATGGGGGCAA GGTGCCCATCAAATGGATGGCATTGGAATCTATTCTCAGACGCCGGTTCACCCATCAGAGTGATGTGTGG AGCTATGGAGTGACTGTGTGGGAGCTGATGACTTTTGGGGCCAAACCTTACGATGGAATCCCAGCCCGGG AGATCCCTGATTTGCTGGAGAAGGGAGAACGCCTACCTCAGCCTCCAATCTGCACCATTGATGTCTACAT GATTATGGTCAAATGTTGGATGATTGACTCTGAATGTCGCCCGAGATTCCGGGAGTTGGTGTCAGAATTT TCACGTATGGCGAGGGACCCCCAGCGTTTTGTGGTCATCCAGAACGAGGACTTGGGCCCATCCAGCCCCA TGGACAGTACCTTCTACCGTTCACTGCTGGAAGATGATGACATGGGTGACCTGGTAGACGCTGAAGAGTA TCTGGTGCCCCAGCAGGGATTCTTCTCCCCGGACCCTACCCCAGGCACTGGGAGCACAGCCCATAGAAGG CACCGCAGCTCGTCCACCAGGAGTGGAGGTGGTGAGCTGACACTGGGCCTGGAGCCCTCGGAAGAAGGGC CCCCCAGATCTCCACTGGCTCCCTCGGAAGGGGCTGGCTCCGATGTGTTTGATGGTGACCTGGCAATGGG GGTAACCAAAGGGCTGCAGAGCCTCTCTCCACATGACCTCAGCCCTCTACAGCGGTACAGCGAGGACCCC ACATTACCTCTGCCCCCCGAGACTGATGGCTATGTTGCTCCCCTGGCCTGCAGCCCCCAGCCCGAGTATG TGAACCAATCAGAGGTTCAGCCTCAGCCTCCTTTAACCCCAGAGGGTCCTCTGCCTCCTGTCCGGCCTGC TGGTGCTACTCTAGAAAGACCCAAGACTCTCTCTCCTGGGAAGAATGGGGTTGTCAAAGACGTTTTTGCC TTCGGGGGTGCTGTGGAGAACCCTGAATACTTAGTACCGAGAGAAGGCACTGCCTCTCCGCCCCACCCTT CTCCTGCCTTCAGCCCAGCCTTTGACAACCTCTATTACTGGGACCAGAACTCATCGGAGCAGGGGCCTCC ACCAAGTAACTTTGAAGGGACCCCCACTGCAGAGAACCCTGAGTACCTAGGCCTGGATGTACCTGTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_017003
Insert Size	3780 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_017003.2, NP_058699.2
RefSeq Size	4727 bp
RefSeq ORF	3780 bp
Locus ID	24337
Gene Summary	may play a role in cell proliferation and differentiation [RGD, Feb 2006]

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