Col4a1 (NM_001135009) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206114 representing NM_001135009 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCCCGGCTCAGCGTCTGGCTTCTGCTGCCGCTCGCCGCCCTTCTGCTCCACGAGGAGCGCAGCC GGGCCGCTGCGAAGGGTGATTGTGGTGGCTCTGGCTGTGGAAAATGTGACTGTCATGGTGTGAAGGGACA AAAGGGAGAGAGAGGCTTTCCAGGGTTACAAGGTGTCATTGGATTTCCTGGGATGCAAGGACCTGAGGGG CCACATGGACCACCAGGACAAAAGGGTGATGCTGGAGAACCTGGACTTCCTGGAACGAAAGGGACACGAG GACCCCCTGGAGCAGCTGGCTACCCTGGGAATCCCGGACTTCCTGGTATTCCTGGCCAGGATGGTCCTCC AGGTCCCCCAGGTATCCCAGGATGCAATGGTACAAAGGGAGAGAGAGGGCCACTCGGTCCTCCTGGCTTG CCTGGATTCAGCGGGAATCCTGGACCACCGGGGTTACCAGGAATGAAGGGAGATCCCGGTGAAATTCTCG GCCATGTTCCTGGGACCCTGCTGAAAGGAGAGAGAGGATTTCCTGGTATCCCTGGGGCGCCGGGCTCACC AGGGTTGCCAGGACTCCAGGGACCTGTCGGTCCTCCGGGATTTACTGGACCACCGGGTCCTCCAGGCCCT CCTGGACCTCCTGGAGAAAAGGGGCAGATGGGATCCAGCTTCCAAGGACCAAAAGGCGACAAGGGAGAAC AAGGGGTCAGTGGTCCCCCGGGAGTACCTGGACAGGCACAAGTTAAGGAAAAAGGAGACTTTGCCCCAAC AGGAGAAAAGGGTCAGAAAGGTGAACCTGGATTCCAGGGAACACCAGGATATGGAGAGAAAGGTGAACCT GGCAAGCCAGGACCTCGGGGAAAACCTGGAAAAGATGGTGAAAAAGGAGAAAAGGGGAGCCCGGGCTTTC CTGGTGAATCCGGATACCCAGGTCTTCCAGGCCGGCAGGGCCCTCAGGGAGAGAAGGGTGAACCTGGACT TCCAGGCCCCCCTGGAACTGTGGTAGGCACCAGGCCATTGGGAGAGAAAGGAGAGCGAGGCTACCCGGGA TCCCCAGGGTTAAGAGGAGAGCCCGGTCCTAAAGGTTACCCTGGATCGCCAGGCCAACCAGGCCCTCCAG GTTTCGCTGTTCCAGGCCAGACTGGTGCTCCGGGCTTCCCAGGTGAAAGAGGAGAAAAAGGTGAAAGGGG ATCTCCAGGTGTGTCTTTACCAGGACCAAGTGGAAGAGATGGAGCCCCAGGGCCTCCTGGCCCTCCTGGC CCCCCTGGGCAACCAGGCCACACGAACGGCATTGTGGAGTGTCAACCTGGACCACCTGGGGACCAGGGTC CTCCAGGAATTCCAGGACAGCCAGGTTTGACAGGCGAAGTTGGACAGAAAGGTCAGAAAGGAGAGAGCTG CCTTGCCTGTGACACAGAAGGACTTCGAGGCCCCCCAGGGCCACAGGGGCCTCCAGGAGAGATTGGTTTC CCTGGACAGCCAGGGGCTAAAGGTGACCGAGGCCTGCCAGGCAGAGATGGTCTTGAAGGATTGCCGGGTC CACAAGGTTCACCGGGGCTCATAGGCCAGCCTGGAGCTAAGGGAGAGCCCGGCGAGATATTTTTTGACAT GCGACTCAAAGGTGACAAAGGAGACCCAGGTTTTCCAGGACAGCCAGGGATGCCAGGAAGAGCAGGAACT CCGGGAAGAGACGGCCACCCAGGACTCCCCGGACCCAAAGGCTCCCCGGGTTCAATAGGATTGAAGGGAG AGAGAGGTCCCCCTGGAGGAGTTGGCTTTCCTGGTAGTCGTGGAGACATTGGCCCTCCTGGACCCCCAGG AGTCGGTCCCATCGGTCCCATTGGTGAAAAAGGACAAGCAGGCCTTCCTGGGGGCCCCGGGTCCCCAGGT CTTCCAGGTCCAAAGGGTGAAGCAGGAAAGGTTGTCCCACTCCCCGGCCCCCCTGGAGCTGCAGGACTTC CAGGATCCCCTGGGTTCCCAGGGCCACAAGGTGACCGAGGCTTCCCAGGAACCCCAGGACGGCCAGGCAA CCCAGGAGAGAAGGGTGCTGTGGGCCAACCAGGAATTGGATTTCCCGGGCTTCCTGGCCCCAAAGGTGTT GATGGCTTGCCTGGAGAGATAGGACGACCGGGGAGTCCAGGTCGCCCTGGATTTAATGGCTTACCTGGCA ACCCAGGACCGCAAGGCCAAAAGGGAGAACCTGGCATTGGGCTTCCAGGACTCAAAGGGCAGCCAGGTCT TCCAGGTATCCCTGGCACACCCGGAGAGAAAGGCAGCATCGGGGGACCTGGCGTTCCAGGAGAACAGGGG TTGACTGGCCCTCCCGGACTCCAGGGGATCAGAGGTGACCCAGGACCTCCTGGGGTTCAAGGCCCTGCAG GTCCACCAGGGGTTCCAGGAATAGGACCACCCGGAGCTATGGGCCCTCCCGGAGGGCAAGGACCACCAGG TTCATCAGGTCCACCTGGAGTTAAAGGAGAGAAGGGATTCCCTGGATTCCCAGGACTGGATATGCCTGGC CCCAAAGGAGATAAAGGCTCTCAAGGACTTCCTGGCCTCACAGGACAGTCAGGGCTCCCTGGCCTCCCTG GACAGCAGGGGACTCCTGGAGTTCCAGGGTTCCCAGGTTCTAAAGGTGAAATGGGCGTCATGGGAACCCC GGGACAACCAGGCTCACCAGGACCAGCAGGCGCCCCAGGGTTACCCGGAGAGAAAGGAGACCATGGCTTT CCGGGCTCCTCGGGACCCAGGGGCGACCCTGGCTTCAAAGGTGATAAAGGTGATGTCGGGCTTCCTGGCA TGCCAGGATCCATGGAGCACGTGGACATGGGCAGCATGAAGGGGCAGAAAGGAGACCAGGGAGAGAAAGG ACAAATTGGACCCACTGGTGATAAAGGTTCCCGAGGAGACCCCGGAACACCAGGAGTGCCCGGGAAGGAT GGGCAGGCAGGGCACCCTGGACAGCCAGGACCTAAAGGTGACCCAGGCCTTAGTGGAATACCAGGATCCC CTGGACTTCCTGGACCCAAAGGATCGGTTGGAGGAATGGGCTTGCCAGGTTCGCCCGGAGAGAAAGGCGT TCCGGGCATCCCTGGCTCACAGGGTGTCCCTGGCTCACCTGGAGAGAAAGGAGCTAAGGGAGAGAAAGGG CAGTCAGGTCTCCCTGGCATTGGAATTCCTGGACGGCCTGGTGACAAGGGAGATCAGGGGCTTGCAGGTT TCCCAGGCAGCCCTGGTGAGAAGGGAGAGAAAGGCAGTGCCGGAACCCCAGGGATGCCAGGGTCCCCAGG CCCAAGAGGCTCTCCAGGGAACATCGGCCATCCAGGAAGCCCAGGCCTGCCTGGAGAAAAAGGAGACAAA GGCCTCCCAGGACTGGATGGTGTTCCCGGTGTCAAAGGAGAAGCAGGTCTTCCTGGGACTCCTGGCCCCA CAGGCCCAGCTGGCCAGAAGGGAGAACCTGGCAGTGATGGAATCCCGGGGTCGGCAGGAGAGAAGGGTGA ACAAGGTTTGCCAGGAAGAGGCTTCCCAGGCTTCCCCGGCACCAAAGGAGACAAAGGTTCCAAGGGTGAA GTGGGTTTCCCTGGCCTTGCCGGAAGTCCTGGGATTCCTGGAGCCAAAGGCGAGCAAGGATTCATGGGTC CTCCCGGCCCTCAAGGACAGCCGGGCTTACCTGGCACTCCTGGCCACCCTGTGGAGGGGCCCAAAGGAGA CCGAGGACCTCAGGGTCAACCCGGCCTGCCAGGGCATCCGGGACCTATGGGGCCACCAGGGTTCCCTGGA ATCGATGGGCCAAAAGGTGACAAGGGAAATCCAGGTTGGCCAGGAGCTCCTGGGGCTCCAGGCCCTAAGG GAGACCCAGGATTCCAAGGCATGCCGGGCATTGGCGGCTCTCCAGGGATCACAGGTTCAAAGGGAGATAT GGGACCGCCTGGCGTTCCAGGATTCCAAGGTCAGAAAGGTCTTCCTGGCTTGCAGGGAGTGAAGGGAGAT CAGGGAGATCAAGGCATACCCGGCCCTAAAGGTCTCCAAGGTCCCCCTGGGCCCCCAGGTCCCTACGATG TCATCAAAGGAGAACCAGGGCTCCCAGGTCCTGAGGGTCCTCCTGGTCTCAAAGGACTTCAAGGACCACC AGGTCCAAAAGGACAGCAAGGTGTGACAGGCTCCGTGGGCTTGCCTGGACCTCCAGGTGTCCCTGGGTTT GATGGTGCCCCTGGCCAGAAAGGAGAGACTGGACCATTTGGACCACCTGGTCCGAGAGGGTTTCCTGGTC CACCAGGCCCCGATGGGCTGCCAGGATCCATGGGTCCCCCGGGTACCCCATCTGTGGACCATGGCTTCCT TGTGACCAGGCATAGTCAGACAACAGATGACCCGCTGTGTCCCCCAGGGACCAAAATTCTTTACCATGGG TACTCTCTGCTCTATGTCCAAGGAAACGAGCGGGCCCATGGTCAGGACTTGGGTACGGCTGGCAGCTGTC TGCGCAAGTTCAGCACCATGCCCTTCCTCTTCTGCAACATCAACAACGTCTGCAACTTCGCCTCCAGGAA CGACTACTCCTACTGGCTGTCCACTCCGGAGCCCATGCCCATGTCCATGGCACCTATCTCTGGGGACAAC ATCCGGCCCTTCATTAGCAGGTGTGCAGTTTGCGAAGCGCCCGCCATGGTGATGGCGGTGCACAGTCAGA CCATCCAGATCCCGCAGTGCCCCAACGGTTGGTCCTCACTGTGGATTGGCTATTCCTTTGTGATGCACAC CAGCGCTGGTGCTGAAGGTTCTGGCCAAGCCCTTGCATCTCCCGGGTCCTGTCTGGAAGAGTTTAGAAGT GCCCCATTCATCGAGTGCCATGGCAGAGGAACGTGCAATTACTACGCGAATGCTTACAGCTTTTGGCTCG CCACCATAGAGAGAAGCGAGATGTTCAAGAAGCCCACGCCGTCCACCTTGAAGGCAGGGGAGCTGCGCAC GCATGTCAGCCGCTGCCAAGTGTGCATGAGAAGAACATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001135009
Insert Size	5010 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001135009.1, NP_001128481.1
RefSeq Size	6579 bp
RefSeq ORF	5010 bp
Locus ID	290905

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