Notch1 (NM_001105721) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	NOTCH; TAN1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206140 representing NM_001105721 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGCGGCTCCTGGCGCCCTTGCTCTGCCTAACGCTGCTGCCCGCGCTCGCCGCAAGAGGCTTGAGAT GCTCCCAGCCAAGTGGGACCTGCCTGAATGGAGGGAGGTGCGAAGTGGCCAACGGCACTGAAGCCTGTGT CTGCAGCGGAGCGTTCGTGGGCCAGCGATGCCAGGACCCCAGCCCTTGCCTCAGCACACCATGTAAGAAT GCTGGAACGTGCTATGTTGTGGACCATGGCGGCATCGTGGACTATGCCTGCAGTTGCCCCCTGGGTTTCT CTGGGCCCCTCTGCCTGACACCTCTGGCCAATGCCTGCCTGGCCAACCCCTGCCGCAACGGGGGGACCTG TGACCTGCTCACTCTCACAGAATACAAGTGCCGGTGCCCGCCAGGGTGGTCAGGAAAGTCATGTCAGCAA GCCGACCCCTGTGCCTCCAACCCCTGTGCCAATGGTGGCCAGTGCCTGCCCTTTGAGTCTTCATACATCT GTGGCTGCCCGCCCGGCTTCCATGGCCCCACCTGCAGACAAGATGTTAACGAGTGCAGCCAGAACCCTGG GTTGTGCCGTCATGGCGGCACGTGCCACAATGAGATTGGCTCCTATCGCTGTGCCTGCCGTGCCACCCAC ACTGGTCCCCACTGCGAGCTGCCCTACGTGCCCTGCAGCCCCTCACCCTGCCAGAACGGAGGCACCTGCC GCCCTACGGGGGACACCACCCACGAGTGTGCCTGCCTGCCAGGCTTTGCTGGACAGAACTGTGAAGAAAA TGTGGATGACTGCCCAGGAAACAACTGCAAGAACGGGGGTGCCTGTGTGGACGGTGTGAATACCTACAAT TGCCGCTGCCCACCGGAGTGGACAGGTCAGTACTGCACAGAGGATGTGGACGAGTGTCAGCTCATGCCCA ACGCCTGCCAGAATGGCGGAACCTGCCACAACTCCCACGGTGGCTACAACTGCGTGTGTGTCAATGGCTG GACTGGTGAGGACTGCAGTGAGAACATTGATGACTGTGCCAGTGCCGCCTGTTTTCAGGGTGCCACCTGC CATGACCGTGTGGCTTCCTTCTACTGCGAGTGTCCACATGGGCGCACAGGCCTGCTGTGCCACCTGAACG ATGCGTGTATCAGCAACCCCTGCAACGAGGGCTCCAACTGCGACACCAACCCTGTCAACGGCAAGGCCAT CTGCACTTGCCCCTCGGGGTACACGGGGCCAGCCTGCAGCCAGGACGTGGATGAGTGCGCTCTAGGTGCC AACCCGTGTGAGCACGCGGGCAAGTGCCTCAACACACTGGGCTCTTTCGAGTGTCAGTGTCTACAGGGCT ACACTGGGCCCCGCTGTGAGATTGATGTCAACGAGTGCATCTCCAACCCATGTCAGAATGATGCCACGTG CCTGGACCAGATTGGGGAGTTTCAGTGTATATGTATGCCAGGTTATGAGGGTGTATACTGTGAGATCAAC ACGGACGAGTGTGCCAGCAGCCCCTGTCTACACAATGGCCGCTGCGTGGACAAGATCAACGAGTTCCTGT GTCAGTGTCCCAAAGGCTTCAGCGGGCACCTGTGCCAGTATGACGTGGATGAGTGCGCCAGCACACCATG CAAGAACGGCGCCAAGTGCCTGGATGGGCCCAACACCTACACCTGCGTGTGCACAGAAGGTTACACGGGG ACCCACTGCGAGGTGGACATTGACGAGTGTGACCCTGACCCCTGTCACTATGGTTTGTGCAAGGATGGTG TGGCCACCTTTACCTGCCTCTGCCAGCCAGGCTACACAGGCCATCACTGTGAGACCAACATTAATGAGTG TCACAGCCAGCCGTGCCGCCATGGCGGCACCTGCCAGGACCGTGACAACTACTACCTCTGCTTATGCCTC AAGGGGACCACAGGACCCAACTGTGAGATCAATCTGGATGACTGTGCGAGCAACCCCTGTGACTCTGGCA CGTGTCTGGACAAGATCGATGGCTACGAGTGTGCGTGCGAGCCAGGCTACACAGGGAGCATGTGTAATGT CAACATTGACGAATGTGCGGGCAGCCCCTGCCACAACGGGGGCACCTGTGAGGATGGCATCGCCGGCTTC ACTTGCCGCTGCCCCGAGGGCTACCACGACCCTACGTGCCTGTCTGAGGTCAACGAGTGCAACAGTAACC CCTGCATCCATGGAGCTTGCCGGGATGGCCTCAATGGATACAAATGTGACTGTGCCCCTGGGTGGAGTGG GACAAACTGTGACATCAACAACAATGAGTGTGAGTCCAACCCTTGTGTCAACGGTGGCACCTGCAAAGAC ATGACCAGTGGCTACGTATGCACCTGCCGAGAAGGCTTCAGTGGCCCTAACTGCCAGACCAACATTAACG AATGTGCTTCCAACCCCTGCCTGAACCAGGGCACCTGCATTGATGATGTCGCTGGGTACAAATGCAACTG CCCTCTGCCCTATACAGGAGCCACATGTGAGGTGGTGTTGGCCCCATGTGCCACCAGCCCCTGCAAAAAC AGTGGGGTATGCAAGGAGTCTGAGGACTATGAGAGCTTTTCCTGTGTCTGTCCCACAGGCTGGCAAGGTC AAACCTGCGAGATCGACATCAATGAGTGTGTGAAAAGCCCGTGTCGCCATGGTGCCTCTTGCCAGAACAC CAATGGCAGCTACCGCTGCCTCTGCCAGGCTGGCTACACGGGTCGCAACTGCGAGAGTGACATCGATGAC TGCCGACCCAACCCATGTCACAACGGGGGTTCCTGCACTGACGGGGTCAACGCGGCCTTCTGCGACTGCC TGCCCGGCTTCCAGGGTGCCTTCTGTGAGGAGGACATCAACGAATGCGCCAGCAATCCATGCCAAAATGG CGCCAACTGCACTGACTGCGTGGACAGCTACACGTGCACCTGCCCCACGGGCTTCAATGGCATCCATTGC GAGAACAACACACCTGACTGTACCGAGAGCTCCTGTTTCAATGGTGGCACCTGTGTGGATGGTATCAACT CCTTCACCTGTCTGTGCCCACCTGGCTTCACGGGCAGCTACTGCCAGTATGACGTCAATGAGTGTGACTC ACGGCCCTGTCTGCATGGTGGCACCTGCCAAGACAGCTATGGTACCTATAAGTGTACCTGCCCACAGGGC TACACTGGTCTCAACTGCCAGAACCTTGTGCGCTGGTGTGACTCAGCTCCCTGCAAGAATGGCGGCAAGT GCTGGCAGACCAACACACAGTACCACTGCGAGTGCCGCAGCGGCTGGACTGGCTTCAACTGCGACGTGCT CAGTGTGTCCTGCGAGGTGGCTGCACAGAAGCGAGGCATCGATGTCACTCTCCTATGCCAGCACGGAGGG CTCTGTGTGGATGAGGAAGACAAGCATTACTGCCACTGCCAGGCAGGATACACGGGCAGCTACTGTGAGG ACGAGGTGGACGAGTGCTCACCTAATCCCTGCCAGAACGGAGCCACCTGCACTGACTATCTCGGTGGCTT TTCCTGCAAGTGTGTGGCTGGGTACCATGGCTCTAACTGCTCTGAGGAGATCAACGAGTGCCTATCCCAA CCCTGCCAGAATGGGGGTACCTGCATTGATCTGACCAACACCTACAAGTGCTCCTGCCCCAGGGGCACAC AGGGTGTACACTGTGAGATCAACGTCGATGACTGCCATCCTCCCCTAGACCCTGCTTCCCGAAGCCCCAA ATGCTTCAATAATGGCACCTGCGTGGACCAGGTGGGTGGCTATACCTGCACCTGCCCGCCAGGCTTCGTC GGGGAGCGGTGCGAGGGCGATGTCAATGAGTGTCTCTCCAACCCCTGTGACCCACGTGGCACCCAGAACT GCGTGCAGCGTGTTAATGACTTCCACTGCGAGTGCCGGGCTGGCCACACTGGACGCCGCTGTGAGTCGGT CATTAATGGCTGCAGGGGCAAACCATGCAGGAATGGAGGTGTCTGTGCTGTGGCCTCCAACACCGCCCGT GGATTCATCTGTAGGTGCCCTGCGGGCTTCGAGGGTGCCACTTGTGAAAATGACGCCCGCACTTGTGGCA GTTTGCGCTGCCTCAACGGTGGTACGTGCATCTCAGGCCCACGCAGTCCCACCTGCCTATGCCTGGGCTC CTTCACTGGCCCTGAATGCCAGTTCCCAGCCAGCAGCCCCTGTGTGGGTAGCAACCCCTGCTACAATCAG GGCACCTGTGAGCCCACATCCGAGAGCCCTTTCTACCGCTGTCTATGCCCTGCCAAATTCAACGGGCTGC TGTGCCACATCCTGGACTACAGCTTCACAGGTGGCGCCGGGCGCGACATTCCCCCGCCACAGATTGAGGA GGCCTGCGAACTGCCTGAGTGCCAGGAGGATGCAGGCAATAAGGTCTGCAACCTTCAGTGTAATAATCAC GCGTGTGGCTGGGACGGCGGCGACTGCTCCCTCAACTTCAATGACCCTTGGAAGAACTGCACTCAGTCCC TGCAGTGCTGGAAGTATTTTAGTGACGGCCACTGTGACAGCCAGTGCAACTCAGCTGGCTGCCTTTTTGA TGGCTTCGACTGCCAGCTCACCGAGGGACAGTGCAACCCCCTGTATGACCAGTACTGCAAGGACCACTTC AGTGATGGCCACTGCGACCAGGGCTGCAACAGTGCCGAGTGTGAGTGGGATGGACTGGACTGTGCAGAGC ATGTCCCCGAGCGACTGGCAGCCGGCACACTGGTGCTGGTGGTGCTGCTTCCGCCCGATCAGCTACGCAA CAACTCCTTCCACTTCCTGCGGGAGCTCAGCCACGTGCTGCACACCAACGTGGTCTTCAAGCGTGACGCG CAGGGCCAGCAGATGATCTTCCCCTACTATGGCCGCGAGGAAGAGCTACGCAAGCACCCGATCAAGCGTT CTGCAGTGGGCTGGGCCACAACGTCACTGCTTCCTGGCACCAATGGTGGGCGCCAGCGCAGGGAGCTGGA CCCCATGGACATCCATGGCTCCATCGTCTACCTGGAGATCGACAACCGGCAGTGTGTACAGTCATCCTCG CAATGCTTCCAGAGCGCCACCGATGTGGCTGCCTTCCTAGGTGCTCTTGCCTCCCTCGGCAGCCTCAATA TCCCCTACAAGATCGAAGCCGTAAAGAGTGAGACGGTGGAGCCTCCGCTGCCCTCACAGCTGCACCTCAT GTACGTGGCGGCAGCTGCCTTCGTGCTCCTGTTCTTTGTGGGCTGTGGGGTGCTGCTGTCCCGCAAGCGC AGGCGGCAGCATGGCCAGCTCTGGTTCCCTGAGGGTTTCAAAGTGTCAGAGGCCAGCAAGAAGAAGCGGA GAGAACCCCTCGGCGAGGACTCAGTCGGCCTCAAGCCCCTGAAGAACGCCTCAGATGGTGCCCTGATGGA CGACAATCAGAACGAGTGGGGGGACGAAGACCTGGAGACCAAGAAGTTCCGGTTTGAGGAACCAGTGGTT CTCCCTGACCTGGATGATCAGACTGACCACCGGCAGTGGACCCAGCAGCACCTGGATGCCGCTGACCTAC GTGTGTCTGCCATGGCCCCAACGCCGCCTCAGGGGGAGGTAGATGCTGACTGCATGGACGTCAATGTTCG AGGACCAGATGGCTTCACTCCCCTAATGATTGCCTCCTGCAGCGGAGGGGGCCTGGAGACAGGCAACAGT GAGGAAGAAGAAGATGCACCTGCTGTCATCTCCGACTTCATCTATCAGGGTGCCAGCTTGCACAACCAGA CGGACCGCACAGGGGAGACTGCCCTGCACCTGGCTGCCCGATACTCTCGTTCAGATGCTGCCAAGCGCTT GCTGGAGGCCAGCGCAGATGCCAACATCCAAGACAACATGGGGCGTACCCCATTACATGCCGCTGTTTCT GCAGACGCTCAGGGTGTCTTCCAGATCCTGCTCCGGAACAGAGCCACAGATCTGGATGCCCGAATGCATG ATGGCACAACCCCTCTGATCCTGGCGGCACGCCTGGCCGTGGAAGGCATGCTAGAGGACCTCATCAACTC TCACGCTGATGTCAATGCTGTGGATGACCTAGGCAAGTCAGCTCTGCACTGGGCAGCCGCTGTGAACAAT GTGGACGCTGCTGTTGTGCTCCTGAAGAACGGAGCCAACAAAGACATGCAGAACAACAAGGAGGAGACTC CCCTGTTCCTGGCCGCCCGTGAGGGCAGCTATGAGACTGCCAAAGTGTTGCTGGACCACTTTGCCAACCG GGACATCACGGATCACATGGACCGATTGCCACGGGACATTGCACAGGAGCGCATGCACCACGATATCGTG CGGCTTTTGGATGAATACAACCTGGTGCGCAGCCCACAGCTGCATGGCACTGCCTTGGGTGGCACACCCA CTCTGTCTCCCACACTCTGCTCGCCCAACGGCTACCTGGGCAACCTCAAGTCTGCCACACAGGGCAAGAA GGCCCGAAAGCCCAGCACCAAAGGGCTGGCTTGCAGTAGCAAGGAAGCTAAGGACCTCAAGGCCCGGAGG AAGAAGTCCCAGGATGGCAAGGGCTGCCTGTTGGACAGCTCAAGCATGCTGTCACCCGTGGACTCCCTCG AGTCACCCCATGGCTACTTGTCAGATGTGGCCTCACCACCCCTCCTTCCCTCCCCGTTCCAGCAGTCTCC ATCCATGCCTCTCAGCCACCTGCCAGGTATGCCTGACACCCACCTGGGCATCAGCCACTTGAATGTGGCA GCCAAGCCCGAGATGGCAGCTCTGGCCGGAGGCAGCCGGTTGGCCTTTGAGCCACCCCCACCACGCCTCT CCCACCTGCCTGTAGCCTCCAGTGCCAGCACAGTGCTGAGTACCAATGGCACAGGGGCTATGAATTTCAC CGTGGGTGCACCGGCAAGCTTGAATGGCCAGTGTGAGTGGCTTCCCCGGCTCCAGAATGGCATGGTGCCC AGCCAGTACAACCCGCTAAGGCCAGGTGTGACTCCGGGCACACTAAGCACACAGGCAGCTGGCCTCCAGC ATGGCATGATGGGCCCGATACACAGCAGCCTCTCCACCAATACCTTGTCCCCGATTATCTACCAGGGCCT GCCCAACACAAGGCTGGCCACACAGCCCCACCTGGTGCAGACCCAGCAGGTGCAGCCACAGAACTTACAA ATCCAGCCTCAGAACCTGCAGCCACCATCGCAGCCACACCTCAGTGTGAGCTCAGCAGCCAATGGGCACC TGGGTCGGAGCTTCCTGAGCGGGGAGCCCAGCCAGGCAGACGTACAGCCGCTGGGCCCCAGCAGTCTGCC TGTGCACACCATTCTGCCCCAGGAAAGCCAGGCTCTGCCGACATCACTGCCATCCTCCATGGTCCCACCC ATGACCACTACCCAGTTCCTGACCCCTCCTTCTCAGCACAGCTACTCATCCTCACCTGTGGACAACACCC CCAGCCACCAGCTGCAGGTGCCAGAGCACCCCTTCCTCACCCCATCCCCTGAGTCCCCTGACCAGTGGTC CAGCTCCTCCCCGCATTCCAACATCTCTGATTGGTCCGAGGGCATCTCTAGCCCGCCCACGAGCATGCCG TCCCAGATCACCCACATTCCAGAGGCATTTAAGTAA ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_001105721
Insert Size	7596 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001105721.1, NP_001099191.1
RefSeq Size	9407 bp
RefSeq ORF	7596 bp
Locus ID	25496
UniProt ID	Q07008
Gene Summary	transmembrane receptor; involved in cell-cell interactions important for development and pattern formation [RGD, Feb 2006]

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