Eif2ak4 (NM_001105744) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206229 representing NM_001105744 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGGGCCGCGGGGCCGCAGGACGCGGCCCGGCTGAGCCGCAGGAGAGCTATTCACAGAGACAGG ACCACGAGCTGCAGGCCCTGGAGGCGATTTACGGCTCGGACTTCCAGGATCTGCGGCCGGATGCGCGCGG ACGGGTGAGAGAGCCTCCTGAAATCAATTTAGTTCTGTACCCTCAGGGCCTGGCTGGTGAGGAAGTGTAC GTGCAGGTGGAGCTGAGGGTCAAATGCCCACCGACATACCCGGATGTAGTTCCCGAAATAGAGTTAAAAA ACACTAAAGGTCTGTCGAATGAGAGTGTTAATTTGTTAAAGTCACACCTGGAAGAACTGGCCAAAAAGCA GTGTGGAGAGGTGATGATATTCGAACTGGCACACCACGTGCAGTCGTTTCTCAGCGAGCATAACAAGCCC CCTCCAAAGTCTTTCCATGAAGAAATGCTGGAAAGGCAGGCTCAGGAGAAGCAGCAGAGGTTGCTGGAGG CCAGGCAGAAAGAGGAGCAGGAGCAACGTGAAATCCTCCACGAGATTCAGAAAAGGAAAGAGGAGATCAA GGAAGAGAAGAAAAGGAAGGAAATGGCTAAGCAGGAACGTTTGGAAATTACTAGTTTGACAAACCAGGAT CATGCCTCTAAGAGAGACCCAGCAGGACACAGGGCAGCTGCCATTCTCCATGGAGGCTCTCCTGACTTTG TAGGAAATGGTAAAGCCCGGGCACACTCCTCAGGAAGGTCCAGGCGAGAACGTCAGTATTCTGTCTGTAG TGGTGAAGCCTCTCCTGGCTCGTGTGACATCCTCTACTTCTGTGTGGGTAGCGCTGATCAGCTCATGGTG CACAAAGGGAAATGTGTTGGCAGCGATGAGCAGCTGGGGAAGGTGGTATACAATGCATTGGAAACAGCCA CGGGCAGCTTTGTCTTGTTGTATGAGTGGGTCCTTCAGTGGCAGAAAAAGATGGGCCCTTGCCTTACCAG TCAAGAGAAAGAGAAGATCGATAAATGTAAAAAGCAGATTCAAGGAGCAGAAACAGAATTCAGCTCCCTA GTGAAACTGAGCCATCCAAACATTGTCCGCTACTTTGCGATGAACTCCAGAGAGGAGAAGGATTCCATTG TGGTCGACATTCTGGCAGAACACATCAGTGGGATCTCTCTCGCTGCACACCTGAGCCACTCAGGCCCTGT GCCCATGCACCAGCTGCGCAAATACACAGCGCAGCTCCTGGCGGGCCTGGACTACCTGCACCGCAACTCG GTGGTGCACAAGGTGCTGAGCACCGCCAGCGTCTTGGTGGATGCTGAGGGCACCGTCAAGATAACAGACT ACAGCATCTCTAAGCGCCTGGCAGACATTTGCAAGGAGGATGTTTTTGAGCAAACTCGAGTTCGCTTTAG TGACAGTGCTCTGCCTTATAAAACCGGGAAGAAAGGGGATGTGTGGCGTCTTGGCCTCCTGCTGTTGTCT CTTAGCCAAGGTCAGGAGTGTGAGGAGTATCCTGTGACCATCCCCAGTGATCTGCCAGCTGACTTCCAGG ACTTCCTGAAGAAGTGTGTTTGCCTGGACGACAAGGAAAGGTGGAGCCCTCAACAGTTGCTGAAACACAG CTTTATAAACCCTCAGCCAAAAATGCCTTTGGTGGAGCAGAGTCCTGAAGATTCTGGGGGACAAGATTAC ATCGAGACCATCATCCCCAGCAACCAGCTGCCCAGCGCTGCGTTCTTCACTGAGACACAGAGACAGTTCT CTCGATACTTCATTGAGTTTGAAGAACTACAGCTTCTGGGGAAAGGAGCTTTTGGAGCTGTCATCAAGGT GCAAAACAAGCTTGATGGCTGTTGCTATGCTGTGAAGCGCATCCCTATCAACCCTGCAAGCAGGCAGTTC CGCAGGATCAAGGGCGAGGTGACGCTGCTATCGCGCCTGCACCATGAGAACATTGTACGCTACTACAACG CCTGGATCGAACGGCATGAGCGCCCAGCGGTGCCAGGGACACCACCCCCAGACTACATACCCCAGGCCCA GAACAGCTCAGCCACCGGCGGGAAAGCATCAGGCGACACTGAGGAACTGGGCAGCGTGGAGGCTGCAGCA CCACCGCCCATCCTCAGCAGCTCAGTGGAGTGGAGCACATCTGCAGAGCGCTCTAACAGCGCCCGTTTCC CAGTCACAGGCCAGGACTCCAGCAGCGATGAGGAAGATGAGGACGAGCGCGATGGTGTCTTCTCACAGTC CTTTCTACCTGCTTCAGATTCTGACAGTGACATCATCTTTGACAATGAAGATGAAAACAGTAAAAGTCAG AATCAGGATGAAGACTGCAATGAAAAGGACAGTCGCCATGAGATTGAGCCATCAGTGACGACCGAGGCTG TGCACTACCTGTACATCCAGATGGAGTACTGCGAGAAGAGCACACTGCGAGACACCATTGACCAGGGGCT GTTCCGAGACACCAGCCGGCTCTGGAGGCTTTTCCGAGAGATTCTGGATGGATTAGCTTATATCCATGAG AAAGGAATGATTCACCGGGATTTGAAGCCTGTCAACATTTTTTTGGATTCTGATGACCATGTGAAAATAG GTGATTTTGGTTTGGCAACAGACCATCTGGCCTTCAATGCTGAAGGTAAACAGGACGACCAAGCAGGTGA TCATGTGATTAAGTCAGACCCTTCAGGCCATTTGACTGGCATGGTTGGCACCGCTCTGTATGTAAGCCCT GAGGTCCAAGGAAGTACCAAGTCTGCGTACAACCAGAAAGTGGATCTCTTCAGCCTGGGAATTATCCTCT TTGAGATGTCGTATCACCCGATGGTGACTGCCTCAGAAAGAATTTTCGTTCTCAACCAACTTCGAGATCC CACGTCACCCAAGTTCCCAGACGACTTTGAGGACGGAGAGCATACAAAGCAGAAATCTGTCATCTCCTGG CTGTTAAACCATGATCCAGCCAAACGGCCCACAGCCATGGAGCTGCTCAAGAGTGAGCTGCTCCCCCCAC CACAGATGGAGGAGTCGGAGCTGCATGAAGTGCTGCACCACACGCTGGCCAACACGGATGGGAAGGCCTA CCGCACCATGATGAGCCAGCTCTTCTGCCAGCACAGCTCCCCCGCCATAGACTACACCTACGACAGCGAC ATCCTGAAGGGCAACTTCTTGATTCGCACAGCCAAGATCCAGCAGCTTGTGTGTGAAACCATCGTCCGCG TCTTCAAAAGGCATGGAGCTGTCCAGCTGTGCACCCCGCTGCTGCTTCCCCGAAACAGGCAAATATATGA GCACAACGAAGCCGCTCTGTTCATGGATCACAGTGGCATGCTGGTGATGCTTCCTTTCGACCTGCGGGTC CCTTTTGCAAGATATGTGGCAAGAAATAACATACTGAATTTAAAGCGATACTGCATAGAGCGGGTGTTCA GACCTAGGAAGTTAGACCGATTTCATCCCAAAGAACTCCTGGAATGTGCATTTGACATCGTCACTTCTAC CGCCAACAGCTCCCTGCCCACTGCTGAAACCATCTACACCATCTATGAAGTCATCCAGGAGTTTCCTGCA CTTCAGGAAAGAAATTACAGCATTTACTTGAACCATACAATGCTCCTAAAGGCAATACTCCTACACTGTG GGATCCCAGAGGATAAACTCAGTCAAGTCTACGTTATTCTGTATGATGCTGTGACAGAGAAGCTGACTAG GAGGGAAGTAGAAGCTAAGTTCTGTAACCTGTCCTTGTCATCTAATAGTCTGTGTCGACTCTATAAGTTC ATCGAGCAGAAGGGGGATTTGCAAGACCTAACGCCGACCATAAACTCACTAATAAAACAGAAAACAGGCA TTGCTCAGCTGGTGAAGTATAGCTTAAAAGACCTAGAGGAAGTTGTCGGGCTGCTGAAGAAACTTGGCGT GAAGTTGCAGGTCTCCATCAACCTGGGCTTGGTTTACAAGGTGCAGCAGCACAATGGCATCATCTTCCAG TTCCTGGCATTCAGCAAACGCAGGCAGAGGGTTGTGCCTGAGATCCTTGCAGCTGGTGGCAGATATGACC TGCTGATTCCCAAGTTCAGAGGCCCACAGGCTCTGGGGCCAGTGCCCACTGCTGTCGGTGTCAGCATAGC CATAGACAAGATATTCGCCGCTGTCCTCAACATGGGAGAGCCTGTTACAGTGAGCTCCTGTGACCTCCTG GTTGTCAGTGCTGGCCAGATGTCCATGTCCAGGGCCATCAACCTAACCCAGAAACTCTGGACGGCCGGCA TCACTGCAGAGATCATGTATGACTGGTCACAGTCCCAGGAAGAGTTACAAGAATACTGCAGACATCACGA AATCACCTATGTGGCCCTGGTCTCTGATAAAGAAGGAAGCCATGTCAAGGTCAAGTCCTTTGAGAAGGAG AGGCAAACAGAAAAACGTGTGTTGGAATCAGATCTTGTGGACCATGTTATGCAGAAACTAAGGACCAAAG TTGGTGACGAAAGGAATTTCAGAGACGCTTCTGATAATCTTGCCGTACAAACTCTGAAGGGGTCGTTTTC TAATGCTTCAGGTTTGTTTGAAATCCATGGAACAACAGTAGTCCCCACTGTGAGTGTTATATCACCAGAA AAACTGTCGGCCAGCACCAGGAGGCGACATGAGATTCAGGTGCAAACCCGACTTCAGACTACCCTTGCCA ATTTACATCAGAAAAGCAGTGAAATTGAAATTTTGGCTGTGGATCTACCCAAGGAGACCATCTTACAGTT TCTATCGCTAGAGTGGGACGCTGATGAACAGGCATTTAACACAACCGTGAAACAGCTGCTGTCACGCCTG CCAAAGCAAAGATACCTCAAACTCGTCTGCGACGAAATCTATAACATCAAAGTTGAGAAGAAGGTGTCGG TGTTGTTCCTGTACAGCTACAGAGATGACTACTACAGAATCCTATTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001105744
Insert Size	4950 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001105744.2, NP_001099214.2
RefSeq Size	5225 bp
RefSeq ORF	4950 bp
Locus ID	114859

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.