Pan2 (NM_001008862) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Usp52
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206390 representing NM_001008862 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTTTGAGGGTCTGGATCCTGGACTGGCCGAATTCTCCCCAGCTATGCATTCTACCCTGGACCCAG TCCTGGATGCCCACCTGAATCCAAGTTTGCTACAGAATGTGGAGCTGGACCCAGAGGGAGTGGCTTTGGA GGCCCTTCCTGTCCAGGAGTCAGTGCACATAATGGAAGGTGTCTACTCTGAGTTGCACAGCGTGGTGGCT GAAGTGGGAGTGCCTGTGTCTGTCTCCCACTTTGATTTGCAGGAGGAGATGCTGTGGGTGGGGAGCCACG GGGGCCATGCCACTTCCTTCTTCGGTCCCGCTCTGGAGCGCTACTCGTCCTTTCAGGTCAATGGCAGTGA TGACATTCGGCAAATCCAGAGCCTGGAGAACGGTATCCTTTTCCTCACCAAGAACAACCTCAAGTACATG GCCCGTGGGGGGCTCATCATCTTTGACTATTTGCTGGATGAGAGTGAGGACATGCACAGTCTCCTCCTGG CGGACAACAACACTCTGCTTGTTGGTGGGCTGCAGAACCACGTCCTGGAGATCGATCTAAACACTGTCCA GGAGACTCAGAAGTATGCAGTCGAGACACCCGGAGTCACCATCATGAGACAGACAAATCGCTTCTTCTTC TGTGGCCACACATCTGGCAAGGTTTCCCTGCGAGACCTCCGTAGTTTTAAAGTGGAACATGAATTTGATG CCTTCTCAGGGAGTCTGTCCGACTTTGATGTTCATGGCAACCTGCTGGCTGCCTGCGGCTTTTCCAGCCG CCTTACCGGCCTGGCCTGTGACCGTTTCCTCAAGGTGTATGACCTGCGCATGATGCGTGCCATCACCCCA CTTCAAGTGCACGTGGATCCAGCCTTCTTGCGTTTCATCCCCACTTACACATCCCGCCTTGCTATCATTT CCCAATCAGGTCAGTGCCAGTTTTGTGAACCCACAGGCCTGGCCAACCCAGCTGACATCTTCCATGTGAA TCCCGTGGGACCTCTGCTAATGACATTTGATGTGTCAGCCAGCAAGCAGGCCCTGGCCTTTGGGGATTCC GAGGGCTGTGTGCATCTCTGGACTGACTCCCCCGAGCCGTCCTTCAACCCCTACTCCCGAGAGACTGAAT TCGCTCTGCCCTGTCTTGTGGACTCATTGCCTGCGCTGGACTGGAGCCAGGATCTGCTGCCGCTTTCCCT CATTCCCGTCCCACTTACCACTGACGCGCTGCTCTCCGACTGGCCGGCTGCCAACTCTGCCCCTGCTCCC AGGCGAGCACCACCTGTGGATGCAGAAATCCTTCGAACCATGAAGAAAGTGGGCTTCATTGGCTACGCCC CCAACCCCCGCACCAGGCTGCGCAACCAGATTCCCTATCGACTAAAGGAGTCAGACCATGAATTTGACAA CTTCAGCCAAGTCACAGAGTCACCGACAGGGCGAGAAGAGGAGCCTCTCCACACAGTTTCTAAGAAATAC CGGAAGGTAACCATCAAATATTCCAAGCTGGGACTGGAAGACTTTGACTTCAAACACTACAATAAGACTC TGTTTGCTGGGTTAGAGCCTCACATCCCCAATGCCTACTGTAACTGCATGATCCAGGTGCTCTATTTCTT GGAGCCTGTTCGCTGTCTGATCCAGAACCACCTTTGCCAGAAGGAGTTCTGCCTGGCATGTGAGCTGGGC TTTCTCTTCCACATGCTGGACCTCTCTCGTGGCGACCCTTGTCAGGGCAGTAATTTCCTCCGAGCTTTCC GGACCATTCCTGAGGCCTCAGCACTGGGTCTCATCCTGGCTGACTCAGATGAGGCTTCAGGCAAGGGCAG CCTGGCCAGGCTCATCCAGAGGTGGAACCGCTTCATCCTCACTCAGCTGCATCAGGATATGCAGGAGCTG GAAGTACCCCAGGCTTATCGAGGTGCTGGGGGCAGTTTCTGCTCATCAGGGGATTCCATCATCGGGCAGC TGTTCAGCTGTGAGATGGAGAACTGTAGCCTCTGCCGCTGTGGCAGTGAGACTGTGCGGGCCTCGTCCAC CCTGCTCTTCACACTCTCCTACCCTGAGGATAAAACCGGCAAGAACTATGACTTTGCTCAGGTGCTGAAG CGAAGTATTTGCCTGGAGCAGAACACACAGGCCTGGTGTGACAGCTGTGAGAAGTACCAGCCTACAATTC AGACCCGCAACATCCGACATCTGCCAGATATTCTTGTCATTAATTGTGAAGTGAATAGCTCAAAAGAGGC TGATTTCTGGAGACTGCAAGCTGAGGTTGCCTTCAAAATAGCAGTGAAGAAGTTCGGCGGGGAAGTAAAG AGCAAGGAGTTTGCTTTAGCTGATAGGAAGGAACTGAGGAGTCCCGAGGGCTTTCTGTGTTGTTCCTCCA TGGAAGAGCTGAAGAACGTCTGGCTTCCATTTTCCATCCGCATGAAGATGACCAAGAACAAAGGACTGGA CGTGTGCAACTGGGCTGATGAGCATGAGCTTAGCAGCCTGGGTACCCCCTCACAGTGGGGTCCAGCCAGG GCAGAGGAGGAGCATGGTGTCTATGTCTATGACCTGATGGCTACTGTGGTACACATCCTGGACTCACGAA CAGGAGGCAGCTTGGTAGCTCACATCAAAGTCGGCGAGACCTACCACCAGCGCAAGGAGGGAGTGACTCA CCAGCAGTGGTATCTCTTCAATGACTTCCTTATTGAACCTATTGATAAGTATGAAGCTGTGCAATTTGAT ATGAACTGGAAAGTACCTGCTATCCTGTATTACGTCAAAAGGAATCTCAATTCCAGATACAACCTGAACA GTAAGAACCCTATTGAGGCTAGCGTGCTGCTGGCAGAAGCCTCACTGGCACGGAAACAACGGAAAACACA CACTACCTTTATCCCACTGATGCTGAATGAGATGCCACAGGTTGGGGACCTGGTAGGCCTTGATGCCGAG TTTGTCACTCTTAATGAGGAAGAAGCAGAGTTACGAAGTGATGGCACCAAGTCCACCATTAAGCCAAGCC AGATGTCAGTAGCAAGGATCACCTGTGTTCGGGGCCAAGGGCCTAACGAGGGTATCCCCTTCATTGATGA CTACATCTCCACACAGGAGCAGGTAGTAGACTACTTGACTCAGTACTCGGGGATAAAGCCAGGAGACCTG GATGCCAAAATTTCCTCAAAGCACCTCACAACTCTCAAGTCGACTTACTTAAAGCTTCGCTTTCTCATTG ACATTGGAGTCAAGTTTGTGGGCCACGGTCTGCAGAAGGACTTCCGGGTCATCAACCTCATGGTACCCAA GGACCAAGTTCTCGACACAGTCTACCTGTTCCACATGCCCCGAAAACGAATGATTTCCCTACGATTCCTT GCTTGGTACTTTCTCGACCTGAAGATTCAAGGTGAGACCCATGACAGTATTGAGGATGCCCGCACAGCCC TTCAGCTCTACCGAAAGTACCTGGAGCTAAGCAAGAACGGCGCTGAGCCCGAGTCCTTCCACAAGGTGCT CAAGGGCCTCTATGAGAAGGGCCGGAAGATGGACTGGAAGGTGCCAGAGCCAGAAAGCCAGAGCAGCCCA AAGAGTAAGGCCGGGCTGAGGCCAGGGGCACTGGGTTGGGTAGGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001008862
Insert Size	3618 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001008862.1, NP_001008862.1
RefSeq Size	3618 bp
RefSeq ORF	3618 bp
Locus ID	408200
UniProt ID	Q6IE70
Gene Summary	Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation.[UniProtKB/Swiss-Prot Function]

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