Ptprk (NM_001029902) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Rptpk
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206411 representing NM_001029902 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTGGCGGCGGCGGCGCTGCCTGCTTTTGTGGCGCTCTGGCTCCTGTCCCCGTGGCCTCTCCTGG GGTCGGTCCAAGGCCAGTTCTCAGCAGGTGGCTGCACTTTTGATGATGGGCCAGGGGCCTGCGACTACCA CCAGGATTTATACGATGACTTTGAGTGGGTCCATGTCAGTGCGCAGGAACCCCATTACCTGCCCCCCGAA ATGCCTCAAGGTTCCTATATGGTTGTGGACTCCTCAAATCATGATCCTGGAGAGAAAGCCCGCCTGCAGC TGCCTACAATGAAGGAGAATGACACTCACTGTATTGATTTCAGCTACCTGTTGTACAGCCAGAAGGGCTT GAACCCGGGCACTTTGAACATCCTGGTTAGGGTGAATAAAGGACCTCTCGCTAATCCAATTTGGAACGTG ACTGGATTCACTGGTCGTGATTGGCTTCGGGCTGAGCTGGCTGTGAGCACCTTTTGGCCTAATGAATACC AGGTAATATTTGAAGCTGAAGTCTCAGGAGGGAGAAGTGGTTATATTGCCATCGACGACATCCAAGTCTT GAGTTATCCTTGCGATAAGTCTCCTCATTTCCTCCGCCTGGGTGATGTGGAGGTCAATGCAGGGCAGAAT GCTACATTTCAGTGCATTGCTACAGGGAGAGATGCTGTACATAATAAGTTATGGCTGCAGAGACGCAATG GAGAAGACATACCTGTAGCCCAGACCAAGAACATCAATCACAGAAGATTTGCTGCCTCTTTCAGATTGCA AGAAGTGACAAAAACCGACCAGGATTTGTACCGCTGCGTAACTCAGTCAGAACGAGGTTCCGGGGTTTCC AATTTTGCCCAGCTTATTGTGAGAGAACCACCTAGACCCATCGCTCCTCCCCAGCTGCTTGGTGTTGGGC CCACTTACTTGCTGATCCAACTAAATGCCAACTCTATTATTGGCGATGGTCCCATCATCCTGAAAGAAGT CGAGTATCGAATGACGTCAGGATCTTGGACGGAAACCCATGCCGTCAATGCTCCAACATACAAGTTATGG CATTTAGATCCAGATACAGAATACGAGATTCGCGTCCTGCTCACCAGACCTGGAGAAGGGGGAACTGGAC TCCCAGGACCACCACTAATCACCAGAACCAAGTGTGCAGAACCCATGCGGACGCCAAAGACTTTGAAGAT TGCTGAAATCCAGGCAAGGCGCATCGCTGTGGACTGGGAGTCCTTGGGCTACAACATCACGCGCTGCCAC ACTTTCAACGTCACCATTTGCTACCATTACTTCCGAGGCCACAATGAGAGCAGGGCCGACTGCTTGGACA TGGACCCCAAAGCCCCTCAACACGTTGTAAACCATCTGCCACCTTACACAAATGTCAGCCTCAAGATGAT CCTAACCAACCCAGAGGGGAGGAAGGAGAGCGAAGAGACAATCATCCAGACTGATGAGGATGTGCCCGGG CCTGTGCCAGTCAAATCCCTCCAAGGAACATCCTTTGAAAACAAGATCTTCCTGAACTGGAAAGAGCCAT TGGAACCAAATGGAATTATCACTCAGTATGAGGTGAGCTATAGCAGCATAAGGTCATTTGACCCTGCTGT CCCAGTGGCTGGGCCCCCGCAGACGGTATCCAATCTGTGGAACAGCACACACCATGTGTTTATGCATCTT CACCCTGGGACCACCTACCAGTTCTTCATAAGAGCCAGCACCGTCAAAGGCTTCGGGCCAGCCACAGCCA TCAACGTGACCACAAATATCTCAGCCCCAAGCTTACCTGACTATGAAGGCGTTGATGCCTCTCTGAATGA AACTGCCACCACCATCACTGTACTGCTGAGACCTGCACAAGCCAAAGGTGCCCCAATCAGTGCTTATCAA ATTGTGGTGGAGCAGCTACACCCACATCGGACCAAGCGTGAAGCCGGGACCATGGAATGCTACCGGGTAC CGGTTACGTACCAGAACTCCCTGAGTGGGGGCGCACCCTATTACTTTGCTGCAGAACTCCCCCCTGGGAA TCTTCCTGAGCCAGCTCCCTTCACCGTGGGCGACAACCGGACCTATAAAGGCTTCTGGAACCCTCCCCTG GCTCCCCGCAAAGGATACAACATCTATTTCCAAGCGATGAGCAGCGTGGAGAAGGAAACTAAAACCCAGT GTGTGCGAATTGCTACAAAAGCAGCAGCAACAGAAGAACCGGAAGTGATCCCAGACCCGGCGAAGCAGAC AGACAGGGTGGTGAAAATCGCGGGCATCAGTGCTGGCGTCTTAGTGTTCATTCTTCTCCTCCTGGTTGTC ATAGTGATTGTGAAAAAGAGCAAGCTAGCTAAGAAGCGCAAAGATGCGATGGGGAACACACGTCAGGAGA TGACCCACATGGTGAATGCTATGGACCGGAGTTACGCTGATCAGAGCACCCTGCATGCAGAAGACCCCCT TTCCCTCACCTTCATGGACCAACATAACTTCAGTCCGAGATATGAAAACCACAGTGCCACAGCAGAGTCT AGTCGTCTCCTCGATGTCCCTCGATACCTCTGTGAAGGGACAGAGTCCCCTTACCAGACAGGACAGCTGC ACCCAGCCATCAGGGTGGCCGACTTACTACAGCACATCAACCTCATGAAGACATCCGACAGCTATGGGTT CAAAGAGGAGTATGAGAGCTTCTTTGAAGGCCAGTCAGCCTCTTGGGACGTGGCTAAAAAGGACCAAAAC AGAGCAAAGAACCGATACGGAAACATTATCGCATACGATCACTCCAGAGTCATCTTGCAGCCCGTGGAGG ACGATCCTTCTTCAGATTACATTAATGCCAACTACATCGATGGCTACCAGAGACCAAGCCACTACATTGC AACTCAAGGACCAGTTCATGAAACCGTGTATGATTTTTGGAGAATGGTATGGCAGGAGCAGTCAGCCTGT ATCGTGATGGTCACTAATTTAGTGGAAGTTGGTCGGGTGAAATGCTATAAATATTGGCCTGATGATACCG AGGTTTACGGTGACTTCAAAGTCACCTGCGTAGAAATGGAACCACTTGCTGAATACGTAGTTAGGACATT CACCTTGGAGAGGAGGGGCTATAACGAAATCCGTGAAGTCAAACAGTTCCACTTCACTGGCTGGCCCGAC CACGGTGTCCCCTACCACGCCACAGGGCTGCTGTCCTTTATCCGAAGAGTCAAGCTGTCTAACCCTCCCA GCGCTGGGCCCATTGTCGTACACTGCAGTGCGGGTGCTGGGCGCACAGGCTGTTACATTGTTATTGACAT CATGCTGGACATGGCTGAAAGAGAGGGTGTGGTCGACATCTACAACTGTGTGAAAGCCTTACGATCTCGC CGCATCAATATGGTCCAGACAGAGGAGCAGTACATTTTTATTCATGATGCCATTTTAGAAGCCTGCTTAT GTGGAGAAACTGCCATCCCTGTGTGCGAATTTAAAGCTGCATATTTTGATATGATTCGAATAGACTCTCA GACTAACTCCTCTCATCTCAAAGACGAATTTCAGACTCTGAATTCGGTCACCCCTCGACTACAAGCTGAA GACTGCAGCATAGCATGCCTGCCAAGGAACCACGACAAGAACCGTTTCATGGACATGCTCCCACCTGACA GATGTCTGCCTTTCTTAATTACAATTGATGGGGAGAGCAGTAACTACATCAATGCTGCTCTTATGGATAG CTATAGGCAACCGGCAGCTTTCATCGTCACACAATACCCGCTGCCGAACACTGTGAAAGACTTCTGGAGA TTAGTATACGATTACGGCTGTACCTCCATTGTGATGTTAAATGAAGTGGACTTGTCCCAGGGCTGTCCAC AGTACTGGCCAGAGGAAGGGATGCTGCGATATGGCCCTATCCAAGTGGAATGCATGTCTTGTTCAATGGA CTGTGACGTGATCAATCGGATTTTTAGGATATGCAACCTAACTAGACCACAAGAAGGCTATCTGATGGTA CAGCAGTTCCAGTACCTAGGCTGGGCTTCTCATCGAGAAGTGCCTGGCTCCAAACGCTCGTTTTTGAAAC TGATACTGCAGGTGGAAAAATGGCAGGAAGAATGTGAAGAAGGAGAAGGCCGGACGATCATCCACTGCTT GAATGGCGGTGGGCGCAGTGGCATGTTCTGTGCAATAGGCATTGTCGTGGAGATGGTGAAGCGGCAAAAT GTGGTGGATGTTTTCCATGCAGTAAAGACCCTGAGGAACAGCAAGCCAAACATGGTGGAAGCTCCGGAGC AGTATCGTTTCTGCTATGACGTGGCTTTGGAGTACCTGGAGTCCTCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001029902
Insert Size	4320 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001029902.2, NP_001025073.2
RefSeq Size	4464 bp
RefSeq ORF	4320 bp
Locus ID	360302
Gene Summary	involved in the stimulation of neurite outgrowth [RGD, Feb 2006]

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