Gapdh (NM_017008) Rat Untagged Clone

CAT#: RN206447

Gapdh (untagged ORF) - Rat glyceraldehyde-3-phosphate dehydrogenase (Gapdh), (10 ug)



  "NM_017008" in other vectors (3)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms BARS-38; Gapd
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN206447 representing NM_017008
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGAAGGTCGGTGTGAACGGATTTGGCCGTATCGGACGCCTGGTTACCAGGGCTGCCTTCTCTTGTG
ACAAAGTGGACATTGTTGCCATCAACGACCCCTTCATTGACCTCAACTACATGGTCTACATGTTCCAGTA
TGACTCTACCCACGGCAAGTTCAACGGCACAGTCAAGGCTGAGAATGGGAAGCTGGTCATCAACGGGAAA
CCCATCACCATCTTCCAGGAGCGAGATCCCGCTAACATCAAATGGGGTGATGCTGGTGCTGAGTATGTCG
TGGAGTCTACTGGCGTCTTCACCACCATGGAGAAGGCTGGGGCTCACCTGAAGGGTGGGGCCAAAAGGGT
CATCATCTCCGCCCCTTCCGCTGATGCCCCCATGTTTGTGATGGGTGTGAACCACGAGAAATATGACAAC
TCCCTCAAGATTGTCAGCAATGCATCCTGCACCACCAACTGCTTAGCCCCCCTGGCCAAGGTCATCCATG
ACAACTTTGGCATCGTGGAAGGGCTCATGACCACAGTCCATGCCATCACTGCCACTCAGAAGACTGTGGA
TGGCCCCTCTGGAAAGCTGTGGCGTGATGGCCGTGGGGCAGCCCAGAACATCATCCCTGCATCCACTGGT
GCTGCCAAGGCTGTGGGCAAGGTCATCCCAGAGCTGAACGGGAAGCTCACTGGCATGGCCTTCCGTGTTC
CTACCCCCAATGTATCCGTTGTGGATCTGACATGCCGCCTGGAGAAACCTGCCAAGTATGATGACATCAA
GAAGGTGGTGAAGCAGGCGGCCGAGGGCCCACTAAAGGGCATCCTGGGCTACACTGAGGACCAGGTTGTC
TCCTGTGACTTCAACAGCAACTCCCATTCTTCCACCTTTGATGCTGGGGCTGGCATTGCTCTCAATGACA
ACTTTGTGAAGCTCATTTCCTGGTATGACAATGAATATGGCTACAGCAACAGGGTGGTGGACCTCATGGC
CTACATGGCCTCCAAGGAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_017008
Insert Size 1002 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_017008.3, NP_058704.1
RefSeq Size 1306 bp
RefSeq ORF 1002 bp
Locus ID 24383
UniProt ID P04797
Gene Summary This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies in human and mouse have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins. Many pseudogenes similar to this locus are found throughout the rat genome. [provided by RefSeq, Jan 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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