Irs1 (NM_012969) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	IRS1IRM
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206729 representing NM_012969 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGCCCTCCGGATACCGATGGCTTCTCAGACGTGCGCAAGGTGGGTTACCTGCGCAAACCCAAGA GTATGCATAAGCGCTTTTTCGTGCTGCGGGCGGCCAGCGAGGCCGGGGGCCCGGCGCGCCTGGAGTATTA TGAGAACGAGAAGAAGTGGCGGCACAAGTCGAGCGCCCCCAAACGCTCGATCCCCCTCGAGAGCTGTTTC AACATCAACAAGCGGGCTGACTCCAAGAACAAGCACCTGGTGGCTCTCTACACCCGAGACGAACACTTTG CCATTGCGGCGGATAGCGAGGCTGAACAAGACAGCTGGTACCAGGCTCTTCTGCAGCTGCATAATCGGGC AAAGGCCCACCATGACGGGGCTGGAGGAGGCTGCGGTGGTAGCTGCAGCGGCAGCTCTGGCGTCGGAGAG GCAGGGGAGGACTTGAGCTATGACACGGGCCCAGGACCCGCGTTCAAGGAGGTCTGGCAGGTTATCCTGA AACCCAAGGGCTTAGGTCAGACAAAGAACTTGATTGGTATCTACCGCCTCTGCCTGACCAGCAAGACCAT CAGCTTTGTGAAGCTCAACTCTGAGGCTGCCGCTGTGGTGCTGCAGCTGATGAACATCAGACGCTGTGGC CACTCAGAGAACTTCTTCTTCATCGAGGTGGGGCGGTCAGCTGTGACCGGGCCCGGCGAGTTCTGGATGC AAGTGGATGACTCCGTGGTGGCCCAGAACATGCATGAGACCATTCTAGAGGCCATGCGGGCCATGAGCGA TGAGTTTCGCCCGCGCACGAAAAGCCAATCTTCATCCAGTTGCTCCAACCCCATCAGTGTTCCCCTGCGC AGGCACCATCTCAACAATCCTCCGCCCAGCCAAGTGGGGCTGACTCGGAGATCTCGAACTGAGAGCATCA CTGCCACCTCCCCTGCCAGTATGGTGGGTGGGAAACCAGGTTCCTTCAGGGTGCGTGCCTCCAGCGATGG CGAAGGCACCATGTCCCGTCCAGCATCAGTGGATGGCAGTCCTGTGAGCCCTAGCACCAACAGGACCCAC GCCCATCGGCATCGAGGCAGCTCCAGGTTGCACCCCCCACTCAACCACAGCCGCTCCATCCCTATGCCTT CTTCACGATGCTCCCCTTCAGCCACCAGCCCAGTGAGCCTGTCATCCAGTAGTACCAGTGGCCACGGCTC CACTTCAGACTGTCTCTTCCCGAGGCGCTCTAGTGCTTCCGTGTCCGGTTCTCCTAGCGATGGCGGTTTC ATCTCTTCTGATGAGTATGGCTCTAGTCCCTGCGATTTCCGAAGTTCCTTCCGCAGTGTCACCCCAGATT CCCTGGGCCACACCCCACCAGCCAGGGGTGAGGAGGAGCTGAGCAACTATATCTGCATGGGTGGCAAGGG AGCCTCCACCTTGACAGCTCCCAATGGTCACTACATTTTGTCTAGGGGTGGCAACGGCCATCGCTACATC CCAGGTGCTACCATGGGGACAAGCCCGGCGCTGACTGGAGACGAAGCCGCTGGTGCAGCAGATCTGGATA ACCGGTTTCGGAAGAGAACTCACTCGGCTGGCACGTCCCCCACCATATCCCACCAGAAGACCCCCTCGCA GTCCTCAGTGGTTTCTATTGAGGAATATACAGAGATGATGCCCGCTGCCTACCCACCAGGAGGTGGCAGT GGAGGCCGACTGCCCGGCTACCGGCATTCCGCCTTCGTGCCCACCCACTCCTATCCCGAGGAGGGTCTAG AGATGCACCACTTGGAACGTCGTGGGGGCCACCACCGTCCAGACTCCTCCAACCTCCACACCGATGATGG CTACATGCCCATGTCTCCCGGAGTGGCTCCAGTGCCCAGCAACCGCAAAGGAAATGGGGACTATATGCCC ATGAGCCCCAAGAGTGTATCTGCCCCCCAGCAGATCATTAACCCCATCAGGCGCCACCCACAGAGAGTGG ACCCCAATGGCTACATGATGATGTCTCCCAGTGGTAGTTGCTCTCCTGACATTGGAGGTGGGTCTTGCAG CAGTAGCAGCATCAGCGCAGCCCCTTCTGGGAGCAGCTATGGGAAGCCATGGACAAACGGAGTAGGGGGG CACCATACCCATGCCCTTCCCCATGCCAAACCTCCTGTTGAGAGCGGTGGTGGTAAGCTCTTGCCTTGCA CTGGTGACTACATGAACATGTCGCCAGTGGGAGATTCCAACACCAGCAGCCCCTCAGAATGCTACTATGG CCCAGAAGATCCCCAGCACAAGCCTGTCCTCTCCTACTACTCATTACCAAGGTCCTTTAAGCACACCCAG CGCCCTGGGGAGCCAGAGGAGGGTGCCAGGCACCAGCATCTTCGTCTCTCTTCAAGCTCTGGACGCCTTC GCTATACCGCAACTGCCGAAGATTCCTCCTCTTCCACCAGCAGCGACAGCCTGGGTGGGGGTTACTGTGG GGCTAGGCCAGAGTCTAGCGTCACACATCCCCACCACCATGCCTTGCAGCCCCATCTGCCTCGAAAGGTA GACACAGCTGCACAGACCAACAGCCGCCTGGCTCGACCCACAAGGCTGTCCTTGGGGGATCCCAAGGCAA GCACTTTACCCCGGGTACGAGAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGTCTTCCCTGCACCCTCC CGAGCCCAAAAGCCCAGGAGAATATGTGAATATTGAATTCGGGAGTGGCCAGCCAGGCTATTTAGCTGGC CCTGCAACTTCCCGTAGCTCCCCTTCAGTTCGATGTCTACCCCAGCTCCACCCAGCTCCCAGAGAAGAGA CTGGCTCGGAAGAGTACATGAACATGGACTTGGGGCCAGGCCGGAGGGCAACCTGGCAGGAGAGTGGTGG AGTTGAGTTGGGCAGAGTAGGCCCTGCACCTCCAGGGGCTGCTTCCATTTGTAGGCCAACCCGGTCGGTG CCAAATAGCCGTGGTGATTACATGACCATGCAGATAGGTTGTCCTCGTCAAAGCTATGTGGATACCTCAC CAGTGGCCCCAGTCAGCTATGCTGACATGCGGACAGGCATTGCTGCAGAGAAGGTGAGCCTGCCCAGAAC CACAGGAGCTGCCCCCCCTCCATCCTCCACAGCCTCTGCTTCTGCTTCTGTTACACCTCAAGGGGCCGCT GAGCAGGCCGCTCACTCTTCCTTGCTGGGAGGCCCTCAGGGACCTGGGGGCATGAGCGCATTCACCAGGG TGAACCTAAGTCCCAACCATAACCAGAGTGCCAAAGTGATTCGTGCAGACACTCAAGGCTGCCGGAGGAG GCACAGCTCCGAGACCTTCTCGGCGCCTACGCGGGCTGCCAACACAGTGTCTTTTGGAGCAGGGGCTGCA GGAGGGGGCAGCGGTGGTGGCAGTGAGGATGTGAAACGCCACAGCTCTGCATCCTTTGAGAATGTGTGGC TGAGACCCGGGGATCTAGGGGGAGCATCCAAGGAGTCGGCTCCAGGGTGCGGGGCTGCCGGGGGATTGGA GAAGAGTCTTAACTATATAGACTTGGATTTGGTCAAGGATGTTAAGCAGCACCCTCAAGACTGCCCCTCT CAACAGCAGTCCCTGCCACCCCCTCCCCCTCACCAACCCTTAGGCAGCAATGAGGGCAGCTCCCCAAGAC GCTCCAGTGAGGATTTAAGCACCTATGCCAGCATCAACTTCCAGAAGCAACCAGAGGACCGTCAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012969
Insert Size	3708 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012969.1, NP_037101.1
RefSeq Size	5365 bp
RefSeq ORF	3708 bp
Locus ID	25467
UniProt ID	P35570
Gene Summary	a docking protein; may act to link the insulin receptor kinase with enzymes regulating cellular growth and metabolism [RGD, Feb 2006]

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