Trpm2 (NM_001011559) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Trpm2-predicted
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN206806 representing NM_001011559 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCTTGGACCAGAGAAGAACTGACTCTGATCAAGAGGAGGGCTTTGGGGTGCAGTCCCGGAGGG CCACTGATCTGGGCATGGTCCCCAATCTCCGACGAAGCAATAGCAGTCTTTGCAAGAGCAGGAGGCTTCT GTGCTCCTTCAGCAGTGAGAAGCAAGAAAACCTTAGCTCATGGATTCCCGAGAACATCAAGAAGAAGGAA TGTGTGTATTTCGTGGAAAGTTCCAAGCTCTCGGATGCAGGGAAGGTAGTGTGTGAGTGTGGTTACACCC ACGAGCAGCACATTGAAGTGGCCATCAAGCCTCACACCTTCCAGGGCAAGGAGTGGGACCCAAAGAAACA CGTCCATGAGATGCCTACAGATGCCTTTGGTGACATTGTCTTCACCGGCCTGAGCCAGAAAGTGGGGAAG TATGTCCGACTCTCCCAGGACACGTCGTCCATTGTCATCTACCAGCTTATGACACAGCACTGGGGCCTGG ATGTCCCCAGCCTCCTCATCTCTGTGACCGGTGGGGCCAAGAACTTCAACATGAAGCTGAGGTTGAAGAG CATCTTCCGGAGAGGCCTGGTTAAGGTGGCCCAAACCACGGGGGCCTGGATCATCACTGGGGGTTCCCAC ACCGGTGTGATGAAGCAGGTGGGCGAGGCGGTACGGGACTTCAGCCTAAGCAGCAGCTGCAAAGAAGGCG ACGTCATCACCATCGGCATAGCCACGTGGGGCACCATCCACAACCGTGAGGCACTGATCCATCCCATGGG AGGCTTCCCCGCTGAGTACATGCTGGATGAGGAAGGCCAAGGGAACCTGACCTGCCTGGACAGCAACCAC TCCCACTTCATCCTGGTGGATGATGGGACCCACGGGCAGTATGGTGTGGAGATTCCGCTGAGGACTAAGC TGGAGAAGTTCATATCGGAGCAAACGAAGGAAAGAGGGGGTGTGGCCATTAAGATCCCCATTGTCTGCGT GGTGTTGGAGGGTGGCCCTGGCACTCTGCATACCATCTACAACGCCATCACCAATGGCACACCCTGCGTG ATAGTGGAGGGCTCCGGCCGAGTGGCTGACGTCATCGCTCAGGTGGCCGCTCTGCCCGTCTCTGAGATCA CCATCTCCCTGATCCAGCAGAAGCTCAGCGTCTTCTTCCAGGAGATGTTTGAGACTTTCACCGAAAACCA GATTGTGGAATGGACCAAAAAGATCCAAGATATTGTCAGGAGGCGGCAGCTGCTGACGGTCTTCCGGGAA GGCAAGGATGGTCAGCAGGATGTGGATGTTGCCATTCTGCAAGCTTTACTGAAAGCCTCTCGAAGCCAAG ATCACTTCGGCCACGAGAACTGGGACCATCAGCTGAAGCTGGCCGTGGCCTGGAACCGTGTGGACATCGC CCGCAGTGAGATCTTCACTGATGAGTGGCAGTGGAAGCCTTCAGACCTGCATCCCATGATGACAGCTGCC CTCATCTCCAACAAGCCTGAGTTTGTGAGGCTCTTTCTGGAGAACGGGGTGCGGCTGAAGGAGTTTGTCA CCTGGGATACTCTTCTCTGCCTCTACGAGAACCTGGAGCCATCCTGCCTTTTCCACAGCAAGCTGCAGAA GGTGCTGGCAGAAGAGCATGAACGCTTAGCCTATGCATCTGAGACACCCCGGCTGCAAATGCACCACGTG GCCCAGGTGCTGCGTGAGCTCCTCGGAGACTCCACACAGCTGCTGTATCCCCGGCCCCGGTACACTGACC GGCCACGGCTCTCGCTGCCCATGCCACACATCAAACTCAACGTGCAAGGAGTGAGCCTCCGGTCTCTCTA TAAGCGATCAACAGGCCACGTTACCTTCACCATTGACCCAGTCCGCGATCTTCTCATTTGGGCCATCATC CAGAACCACAGGGAGCTGGCGGGCATCATCTGGGCTCAGAGCCAGGACTGCACAGCAGCCGCACTGGCCT GCAGCAAGATCCTGAAGGAGCTGTCCAAGGAGGAGGAAGATACAGACAGCTCTGAGGAGATGCTGGCACT CGCGGATGAGTTTGAGCATAGAGCTATCGGTGTCTTCACCGAGTGCTACAGAAAGGATGAGGAAAGAGCC CAGAAGCTGCTTGTCCGTGTGTCTGAGGCCTGGGGGAAGACCACCTGCCTGCAGCTGGCCCTCGAGGCCA AGGACATGAAATTCGTGTCTCACGGAGGGATCCAGGCTTTCCTAACGAAGGTGTGGTGGGGTCAGCTCTG CGTGGACAATGGCCTGTGGAGGATCATCCTGTGCATGCTGGCCTTCCCTCTGCTCTTCACCGGCTTCATC TCCTTCAGGGAAAAGAGGCTGCAGGCACTGTGCCGCCCGGCCCGCGTCCGCGCCTTCTTCAACGCGCCGG TGGTCATCTTCTACCTCAATATTCTCTCCTACTTTGCCTTCCTCTGCCTGTTTGCCTACGTGCTCATGGT GGACTTCCAGCCCTCACCATCCTGGTGCGAGTACCTCATCTACCTGTGGCTCTTCTCCCTGGTGTGCGAG GAGACACGGCAGCTATTCTACGATCCCGATGGCTGCGGGCTCATGAAGATGGCGTCCCTGTACTTCAGTG ACTTCTGGAACAAACTGGACGTTGGGGCCATTCTGCTCTTTATAGCAGGACTGACCTGCCGACTCATCCC AGCGACGCTGTACCCTGGGCGCATCATCCTGTCTTTGGACTTCATTATGTTCTGCCTCCGCCTCATGCAC ATCTTCACCATTAGCAAGACACTGGGGCCCAAGATAATCATCGTGAAGCGGATGATGAAGGACGTCTTCT TCTTCCTCTTTCTCCTGGCGGTGTGGGTGGTGTCCTTCGGAGTGGCCAAGCAGGCCATCCTCATCCACAA TGAGAGCCGCGTGGACTGGATCTTCCGCGGAGTTATCTATCACTCTTACCTTACCATCTTCGGGCAGATC CCGACCTACATTGACGGCGTGAATTTCAGCATGGACCAGTGCAGCCCCAATGGTACAGACCCCTACAAGC CCAAGTGTCCTGAGAGTGACTGGACAGGGCAGGCACCCGCCTTCCCCGAGTGGCTGACAGTCACCCTTCT CTGCCTCTACCTGCTCTTCGCCAACATCCTGCTGCTTAATTTGCTCATCGCCATGTTCAACTACACCTTC CAGGAGGTGCAGGAGCACACAGACCAGATCTGGAAGTTCCAGCGCCACGACCTGATTGAGGAGTACCACG GCCGGCCCCCGGCCCCTCCCCCACTCATCCTCCTCAGCCACCTGCAGCTCCTGATCAAGAGGATTGTCCT GAAGATCCCCGCCAAGAGGCACAAGCAGCTCAAGAACAAGCTGGAGAAGAATGAGGAGGCAGCCCTCCTG TCCTGGGAGCTCTACCTGAAGGAGAATTACCTGCAGAACCAACAGTACCAGCACAAACAGCGGCCAGAGC AGAAGATCCAGGACATCAGTGAGAAAGTGGACACCATGGTGGATCTGTTGGACATGGACCGTGTGAAGAG ATCAGGCTCCACAGAGCAGAGGCTGGCCTCCCTTGAGGAACAGGTGACTCAGATGGGCAGATCTTTGCAC TGGATCGTGACGACCCTGAAGGACAGTGGCTTTGGCTCAGGGGCCGGTGCACTGACCCTGGCAGCCCAAA GGGCCTTCGACGAGCCAGATGCTGAGCTGAGTATCAGGAAGAAAGGAGAGGAGGGAGGAGATGGCTATCA TGTGAGCGCCCGGCACCTCCTCTACCCTGATGCCCGCATCATGCGCTTCCCCGTGCCTAATGAGAAGGTG CCTTGGGAGGCAGAGTTTCTGATCTACGACCCTCCGTTTTACACAGCTGAGAAGAAGGATGCGACTCTCA CAGACCCTGTGGGAGACACTGCAGAACCTCTGTCTAAGATCAATTACAACGTCGTGGACGGACTGATGGA CCGTTGCAGCTTCCATGGGACCTATGTGGTCCAATATGGATTCCCTTTGAACCCCATGGGCCGCACCGGG TTGCGTGGTCGTGGGAGCCTCAGCTGGTTTGGTCCCAACCACACTCTGCAGCCAGTTGTTACCCGGTGGA AGAGGAACCAGGGTGGAGGCATCTGCCGGAAGAGTGTCAGGAAGATGTTGGAGGTGCTGGTCATGAAGTT GCCTCAATCCGAGCACTGGGCCTTGCCTGGGGGCTCTCGGGAGCCAGGGAAGATGCTACCACGGAAGCTG AAACAGGTCCTCCAGCAGGAGTACTGGGTGACCTTTGAGACCTTGCTAAGGCAAGGTACAGAGGTGTACA AAGGATACGTGGATGACCCAAGGAACACGGACAATGCCTGGATCGAGACGGTGGCTGTCAGCATCCATTT CCAGGACCAGAATGATGTGGAGCTGAAGAGGCTGGAAGAGAACCTGCAAACTCATGATCCAAAGGAGTCG GCCCGTGGCTTGGAGATGTCTACTGAATGGCAGGTTGTAGACCGGCGGATCCCTCTGTATGTGAACCACA AGAAGATCCTCCAAAAGGTGGCCTCGCTGTTTGGCGCTCACTTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_001011559
Insert Size	4527 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001011559.1, NP_001011559.1
RefSeq Size	4527 bp
RefSeq ORF	4527 bp
Locus ID	294329
UniProt ID	E9PTA2
Gene Summary	Nonselective, voltage-independent cation channel that mediates Na(+) and Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels (PubMed:16651700, PubMed:16260005, PubMed:11804595, PubMed:16601673, PubMed:19454650). Functions as ligand-gated ion channel. Binding of ADP-ribose to the cytoplasmic Nudix domain causes a conformation change; the channel is primed but still requires Ca(2+) binding to trigger channel opening. Extracellular calcium passes through the channel and increases channel activity (By similarity). Also contributes to Ca(2+) release from intracellular stores in response to ADP-ribose (PubMed:19454650). Plays a role in numerous processes that involve signaling via intracellular Ca(2+) levels (Probable). Besides, mediates the release of lysosomal Zn(2+) stores in response to reactive oxygen species, leading to increased cytosolic Zn(2+) levels (PubMed:25562606). Activated by moderate heat (35 to 40 degrees Celsius) (PubMed:16601673). Activated by intracellular ADP-ribose, beta-NAD (NAD(+)) and similar compounds, and by oxidative stress caused by reactive oxygen or nitrogen species (PubMed:16260005, PubMed:16601673, PubMed:25562606). The precise physiological activators are under debate; the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate. Activation by ADP-ribose and beta-NAD is strongly increased by moderate heat (35 to 40 degrees Celsius) (By similarity). Likewise, reactive oxygen species lower the threshold for activation by moderate heat (37 degrees Celsius). Plays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis. Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca(2+) levels (PubMed:16601673). Required for normal IFNG and cytokine secretion and normal innate immune immunity in response to bacterial infection. Required for normal phagocytosis and cytokine release by macrophages exposed to zymosan (in vitro). Plays a role in dendritic cell differentiation and maturation, and in dendritic cell chemotaxis via its role in regulating cytoplasmic Ca(2+) levels (By similarity). Plays a role in the regulation of the reorganization of the actin cytoskeleton and filopodia formation in response to reactive oxygen species via its function in increasing cytoplasmic Ca(2+) and Zn(2+) levels (By similarity). Confers susceptibility to cell death following oxidative stress (PubMed:16651700, PubMed:11804595, PubMed:19454650, PubMed:25562606).[UniProtKB/Swiss-Prot Function]

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