Afap1 (NM_080900) Rat Untagged Clone

CAT#: RN206817

Afap1 (untagged ORF) - Rat actin filament associated protein 1 (Afap1), (10 ug)



  "NM_080900" in other vectors (3)

CNY 9,310.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Afap
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN206817 representing NM_080900
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGAGTTAATAGTTGAACTCCGCCTGTTCCTGGAGCTCCTGGACCATGAGTACCTCACGTCAACTG
TCAGAGAGAAAAAGGCAGTGCTTACCAACATCCTGCTTCGAATGCAGTCATCCAAGGGCTTTGAAGTGAA
GGACCATGCACAGAAGGCAGAGACCAACAACCTCCCGGCACCTCCTCAGATGCCTCTGCCAGAGATCCCT
CAGCCATGGCTGCCTCCTGACAGTGGGCCTCCGCCGTTACCGACATCCTCTCTTCCAGAGGGTTACTATG
AGGAAGCTGTGCCCCTGAGTCCTGGAAAAGCTCCAGAGTACATCACCTCAAATTATGACTCTGAGGCCAT
GGGCAGTTCCTATGAGTCATATGATGAAGAGGAAGAGGATGGGAAGGGGAAGAAAACGCAGCATCAGTGC
CATCATGAGGAGGCCTCTATGGACCTGGTGAAAGATGCCAAGATCTGCGCCTTCCTGTTGCGTAAGAAAC
GCTTCGGCCAGTGGACCAAGCTGCTCTGCGTCATCAAGGACACCAAGCTGCTGTGCTACAAAAGTTCCAA
GGACCAGCAGCCTCAGATGGAGCTGCCCCTGCAGGGCTGCAGCATCACGTACATCCCGAGAGACAGCAAG
AAGAAGAAACACGAGCTGAAAATCACCCAGCAAGGCACAGACCCTCTGGTCCTCGCCGTCCAGAGCAAGG
AGCAGGCCGAGCAGTGGCTGAAGGTAATCAAAGAGGCCTACAGTGGCTGCAGTGGGCCCGTGGATCCAGA
GTGTTCTCCACCACCCAGCGCCAGTGTCCCTGTGAACAAGGCAGAACTGGAGAAGAAATTGTCTTCAGAG
AGGCCCAGCTCTGATGGAGAAGGTGTTGTAGAAAACGGAGTCACCACGTGTAATGGAAAAGAACAAGCAA
AGAGGAAGAAACCCTCCAAGTCAGAGGCCAAGGGGACTGTGTCCAAGGTCACTGGGAAGAAAATTACCAA
GATCATCGGTCTGGGAAAGAAGAAGCCATCCACAGATGAGCAGACGTCATCAGCAGAGGAAGATGTGCCC
ACCTGTGGCTACCTAAATGTGCTCTCTAACAGTCGCTGGCGGGAGCGCTGGTGCAGAGTGAAGGACAGCA
AGCTCATTCTCCACAAGGACCGGGCTGACCTGAAGACCCACATCGTCTCCATCCCTCTCCGCGGCTGCGA
GGTGATCCCGGGCTTGGATTCTAAGCACCCATTGACCTTCCGGCTGCTTCGAAATGGACAGGAGGTGGCT
GTCCTAGAGGCATCATCTTCTGAAGACATGGGCAGATGGATTGGCATCTTACTGGCTGAGACAGGGTCCT
CAACGGACCCAGGAGCCCTACACTATGACTACATAGATGTGGAGATGTCAGCAAATGTCATCCAGACGGC
TAAACAGACCTTCTGCTTTATGAACAGGAGGGCAGTGTCTACTAGCCCGTACCTAGGGAGCCTCTCCAAT
GGCTATGCCCATCCCAGCGGGACAGCACTACACTATGACGACGTCCCTTGTGTCAACGGCTCGTTGAAGA
ACAAAAAACCTCCAGCGTCATCTAATGGGCTTCCTGTGAAGGGAAGGGCTCCCAGCAGCCAGCAAAAAAA
GGTGGAATCTGCGGGGGGCGTGAAGCGCACAGCATCAAATGCCGAGCAGTACAAATATGGTAAGAACCGG
GTAGAAGCAGATGCCAAGCGGCTACAGTCCAAAGAGGAAGAGCTACTGAAGAGAAAAGAGGCCCTGCGGA
ACAGGCTGGCACAGCTCCGGAAAGAGAGGAAGGACCTGAGGGCTGCCATCGAAGTGAATGCAGGCAGGAA
GACCCAGGTGGCCCTGGAGGACAAGTTGAAGAGGTTGGAGGAAGAGTGCAAGCAGAGGGAGGCAGAGCGG
GTCAGCCTGGAGCTGGAGCTGACGGAGGTCAAGGAGAGCCTGAAGAAGGCCTTGGCAGGTGGCGTCACTC
TAGGACTGGCTATCGAACCCAAGTCAGGGACATCAAGCCCACAGTCTCCAGTGTTTCGGCATCGGACTCT
GGAAAACTCCCCCATCTCCAGCTGTGATACAAGCGATGCTGAGGGGCCTTTGCCTGTGAACAGTGCAGCC
GTCCTGAAGAAGAGCCAGCCGTCCTCCAGCAGCTCCCCTTGCCGTGGGCACGTGCTGCAGAAGGCCAGGG
AGTGGGAGCTGAAGAATGGAACATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_080900
Insert Size 2196 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_080900.1, NP_543176.1
RefSeq Size 2911 bp
RefSeq ORF 2196 bp
Locus ID 140935
UniProt ID Q8VH46
Gene Summary Can cross-link actin filaments into both network and bundle structures. May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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