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Nefl (NM_031783) Rat Untagged Clone
CAT＃: RN207028
Nefl (untagged ORF) - Rat neurofilament, light polypeptide (Nefl), (10 ug)
CNY 6,940.00
货期*
6周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | NF-L; NF68; Nfl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>NM_031783 ORF sequence, RN207028 may differ due to SNPs.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTTCGTTCAGCTACGAGCCGTACTTTTCGACCTCCTACAAGCGGCGCTACGTGGAGACGCCCCGG GTGCACATCTCCAGCGTGCGCAGCGGCTACAGCACGGCGCGCTCTGCGTACTCCAGCTACTCCGCGCCC GTCTCCTCCTCTCTGTCGGTGCGCCGCAGCTACTCATCCAGCTCCGGCTCTTTGATGCCCAGCCTGGAG AACCTCGATCTGAGCCAGGTAGCCGCCATCAGCAACGACCTCAAGTCTATCCGCACACAGGAGAAGGCA CAGCTGCAGGACCTCAACGATCGCTTCGCCAGCTTCATCGAGCGCGTGCACGAGCTGGAGCAGCAGAAC AAGGTCCTGGAAGCCGAGCTGTTGGTGCTGCGCCAGAAGCACTCAGAGCCTTCCCGCTTCCGCGCCCTG TATGAGCAGGAGATCCGTGATCTGCGACTGGCGGCCGAAGACGCCACTAACGAGAAGCAGGCGCTGCAG GGCGAGCGCGAGGGGCTGGAGGAGACTCTGCGCAACCTGCAGGCTCGCTACGAGGAGGAGGTGCTGAGC CGCGAGGACGCCGAGGGCCGGCTGATGGAAGCCCGCAAAGGCGCGGATGAGGCTGCGCTCGCCCGCGCC GAGCTGGAGAAGCGCATCGACAGCCTGATGGACGAGATAGCCTTCCTGAAAAAGGTGCACGAGGAAGAG ATCGCCGAGCTGCAGGCTCAGATCCAGTATGCTCAGATCTCCGTGGAGATGGACGTGTCCTCCAAGCCC GACCTCTCCGCCGCTCTCAAGGACATCCGCGCTCAGTACGAGAAGCTGGCCGCCAAGAATATGCAGAAT GCCGAAGAGTGGTTCAAGAGCCGCTTCACGGTGCTAACCGAGAGCGCCGCCAAGAACACCGACGCAGTG CGCGCTGCCAAGGACGAGGTGTCGGAAAGCCGCCGCCTACTTAAGGCTAAGACCCTGGAGATCGAAGCC TGCCGGGGTATGAACGAAGCTCTAGAGAAGCAGCTGCAGGAGCTGGAGGACAAGCAGAATGCAGACATC AGCGCCATGCAGGACACAATCAACAAACTGGAGAATGAGCTGCGAAGCACGAAGAGCGAGATGGCCAGG TACCTGAAGGAGTACCAGGACCTCCTCAATGTCAAGATGGCATTGGACATTGAGATTGCAGCTTACAGG AAACTCTTGGAAGGCGAAGAAACCAGGCTCAGTTTCACCAGCGTGGGTAGCATAACCAGCGGCTACTCT CAGAGCTCGCAGGTCTTTGGCCGTTCTGCTTACAGTGGCTTGCAGAGCAGCTCCTACTTGATGTCTGCT CGAGCATTCCCAGCCTACTATACCAGCCACGTCCAGGAAGAGCAGTCAGAGGTGGAGGAGACCATTGAG GCTACGAAAGCTGAGGAGGCCAAGGATGAGCCCCCCTCTGAAGGAGAAGCAGAAGAGGAGGAGAAGGAG AAAGAGGAGGGGGAGGAAGAGGAAGGTGCTGAGGAGGAAGAAGCTGCCAAGGATGAGTCTGAAGATGCC AAAGAAGAAGAAGGTGGTGAGGGTGAAGAGGAAGACACCAAAGAGTCAGAAGAGGAAGAGAAGAAAGAG GAAAGTGCTGGGGAGGAGCAAGCTGCTAAGAAGAAAGATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC MSSFSYEPYFSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLE
NLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRAL YEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARA ELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQN AEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADI SAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYS QSSQVFGRSAYSGLQSSSYLMSARAFPAYYTSHVQEEQSEVEETIEATKAEEAKDEPPSEGEAEEEEKE KEEGEEEEGAEEEEAAKDESEDAKEEEGGEGEEEDTKESEEEEKKEESAGEEQAAKKKD* TRTRPLEQKLISEEDLAANDILDYKDDDDKV >RN207028 representing NM_031783
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCGTTCAGCTACGAGCCGTACTTTTCGACCTCCTACAAGCGGCGCTACGTGGAGACGCCCCGGG TGCACATCTCCAGCGTGCGCAGCGGCTACAGCACGGCGCGCTCTGCGTACTCCAGCTACTCCGCGCCCGT CTCCTCCTCTCTGTCGGTGCGCCGCAGCTACTCATCCAGCTCCGGCTCTTTGATGCCCAGCCTGGAGAAC CTCGATCTGAGCCAGGTAGCCGCCATCAGCAACGACCTCAAGTCTATCCGCACACAGGAGAAGGCACAGC TGCAGGACCTCAACGATCGCTTCGCCAGCTTCATCGAGCGCGTGCACGAGCTGGAGCAGCAGAACAAGGT CCTGGAAGCCGAGCTGTTGGTGCTGCGCCAGAAGCACTCAGAGCCTTCCCGCTTCCGCGCCCTGTATGAG CAGGAGATCCGTGATCTGCGACTGGCGGCCGAAGACGCCACTAACGAGAAGCAGGCGCTGCAGGGCGAGC GCGAGGGGCTGGAGGAGACTCTGCGCAACCTGCAGGCTCGCTACGAGGAGGAGGTGCTGAGCCGCGAGGA CGCCGAGGGCCGGCTGATGGAAGCCCGCAAAGGCGCGGATGAGGCTGCGCTCGCCCGCGCCGAGCTGGAG AAGCGCATCGACAGCCTGATGGACGAGATAGCCTTCCTGAAAAAGGTGCACGAGGAAGAGATCGCCGAGC TGCAGGCTCAGATCCAGTATGCTCAGATCTCCGTGGAGATGGACGTGTCCTCCAAGCCCGACCTCTCCGC CGCTCTCAAGGACATCCGCGCTCAGTACGAGAAGCTGGCCGCCAAGAATATGCAGAATGCCGAAGAGTGG TTCAAGAGCCGCTTCACGGTGCTAACCGAGAGCGCCGCCAAGAACACCGACGCAGTGCGCGCTGCCAAGG ACGAGGTGTCGGAAAGCCGCCGCCTACTTAAGGCTAAGACCCTGGAGATCGAAGCCTGCCGGGGTATGAA CGAAGCTCTAGAGAAGCAGCTGCAGGAGCTGGAGGACAAGCAGAATGCAGACATCAGCGCCATGCAGGAC ACAATCAACAAACTGGAGAATGAGCTGCGAAGCACGAAGAGCGAGATGGCCAGGTACCTGAAGGAGTACC AGGACCTCCTCAATGTCAAGATGGCATTGGACATTGAGATTGCAGCTTACAGGAAACTCTTGGAAGGCGA AGAAACCAGGCTCAGTTTCACCAGCGTGGGTAGCATAACCAGCGGCTACTCTCAGAGCTCGCAGGTCTTT GGCCGTTCTGCTTACAGTGGCTTGCAGAGCAGCTCCTACTTGATGTCTGCTCGAGCATTCCCAGCCTACT ATACCAGCCACGTCCAGGAAGAGCAGTCAGAGGTGGAGGAGACCATTGAGGCTACGAAAGCTGAGGAGGC CAAGGATGAGCCCCCCTCTGAAGGAGAAGCAGAAGAGGAGGAGAAGGAGAAAGAGGAGGGGGAGGAAGAG GAAGGTGCTGAGGAGGAAGAAGCTGCCAAGGATGAGTCTGAAGATGCCAAAGAAGAAGAAGGTGGTGAGG GTGAAGAGGAAGACACCAAAGAGTCAGAAGAGGAAGAGAAGAAAGAGGAGAGTGCTGGGGAGGAGCAAGC TGCTAAGAAGAAAGATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_031783 |
| Insert Size | 1629 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_031783.2 |
| RefSeq Size | 2026 bp |
| RefSeq ORF | 1629 bp |
| Locus ID | 83613 |
| UniProt ID | P19527 |
| MW | 61.3 kDa |
| Gene Summary | one component of the hamartin-tuberin tuberous sclerosis complex; may play a role in in central nervous system function [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR207028 | Nefl (Myc-DDK-tagged ORF) - Rat neurofilament, light polypeptide (Nefl), (10 ug) |
CNY 6,940.00 |
|
| RR207028L3 | Lenti ORF clone of Nefl (Myc-DDK-tagged ORF) - Rat neurofilament, light polypeptide (Nefl), (10 ug) |
CNY 6,840.00 |
|
| RR207028L4 | Lenti ORF clone of Nefl (mGFP-tagged ORF) - Rat neurofilament, light polypeptide (Nefl), (10 ug) |
CNY 9,690.00 |
Customer
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