Fbl (NM_001025643) Rat Untagged Clone

CAT#: RN207477

Fbl (untagged ORF) - Rat fibrillarin (Fbl), (10 ug)



  "NM_001025643" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms MGC116371
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN207477 representing NM_001025643
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCCAGGTTTCAGCCCCCGTGGGGGCGGCTTTGGTGGCAGAGGCGGCTTTGGTGACAGAGGTGGTA
GAGGAGGAGGCCGAGGAGGGAGAGGAGGTTTTGGCGGTGGACGAGGAGGCTTTGGCGGTGGAGGTCGAGG
TCGAGGTGGAGGAGGCGGTGGCTTCAGGGGACGAGGAGGTGGAGGTGGACGAGGTGGAGGCTTCCAGTCT
GGGGGCGGCCGGGGTCGAGGTGGTGGCCGGGGAGGCAAGAGAGGAAACCAGTCAGGGAAGAATGTGATGG
TGGAGCCCCATCGGCATGAAGGAGTCTTTATCTGTCGCGGAAAGGAGGATGCCCTTGTCACAAAGAATCT
GGTTCCTGGAGAATCTGTGTATGGAGAGAAGAGAGTCTCTATCTCCGAAGGAGATGACAAAATTGAGTAC
CGAGCCTGGAACCCCTTCCGCTCCAAGCTGGCCGCAGCAATCCTGGGTGGCGTAGACCAGATCCACATCA
AGCCGGGGGCCAAGGTGCTCTACCTTGGGGCAGCCTCAGGCACCACCGTCTCCCACGTGTCTGACATTGT
TGGCCCGGATGGTCTGGTCTACGCAGTTGAGTTCTCCCACCGCTCTGGCCGTGACCTCATCAACTTGGCC
AAGAAGAGGACCAACATTATTCCTGTAATTGAAGATGCTCGGCACCCACACAAATACCGCATGCTTATTG
CAATGGTGGATGTCATCTTTGCCGATGTGGCCCAGCCAGACCAAACCCGAATTGTGGCCCTGAATGCCCA
CACCTTCCTGCGGAATGGAGGACACTTTGTGATTTCCATTAAGGCCAACTGCATTGACTCCACAGCCTCA
GCAGAAGCTGTGTTTGCATCTGAAGTGAAAAAGATGCAGCAAGAGAACATGAAGCCTCAGGAGCAGTTGA
CACTAGAGCCTTATGAACGAGACCACGCTGTGGTTGTAGGTGTGTACAGGCCACCTCCCAAGGCGAAGAA
CTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001025643
Insert Size 984 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001025643.1, NP_001020814.1
RefSeq Size 1208 bp
RefSeq ORF 984 bp
Locus ID 292747
UniProt ID P22509
Gene Summary S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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