Ihh (NM_053384) Rat Untagged Clone

CAT#: RN207510

Ihh (untagged ORF) - Rat Indian hedgehog (Ihh), (10 ug)



  "NM_053384" in other vectors (3)

CNY 3,990.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN207510 representing NM_053384
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCCCGCCTGGCTCCGGCCCCGACTGCGCTTCTGTCTGCTCCTGCTGCTGCTGCTTCTGGTGCCGG
CGGCGCGGGGCTGCGGGCCGGGCCGGGTGGTGGGCAGCCGCCGGAGGCCGCCTCGTAAACTCGTGCCTCT
TGCCTACAAGCAGTTCAGCCCCAACGTGCCGGAGAAGACCCTGGGCGCCAGCGGGCGCTACGAAGGCAAG
ATCGCGCGCAGCTCTGAGCGCTTCAAGGAGCTCACCCCCAACTACAATCCCGACATCATCTTCAAAGACG
AGGAGAACACCGGTGCCGACCGCCTCATGACCCAGCGCTGCAAGGACCGTCTGAACTCACTGGCCATCTC
TGTCATGAACCAGTGGCCGGGTGTGAAGCTGCGGGTGACGGAAGGCTGGGATGAAGACGGCCATCACTCA
GAGGAATCTTTACACTATGAGGGCCGCGCGGTGGATATCACCACCTCAGACCGCGACCGAAATAAGTACG
GACTACTGGCGCGCTTAGCAGTGGAGGCCGGCTTCGACTGGGTGTATTACGAGTCCAAGGCCCACGTTCA
TTGCTCTGTCAAGTCTGAGCACTCGGCTGCTGCCAAGACAGGTGGCTGCTTTCCTGCCGGAGCCCAGGTC
CACCTAGAAACTGGGGAGCGTGTGGCCCTGTCAGCTGTGAAGCCAGGAGACCGGGTCCTGGCCATGGGGG
AAGATGGCAACCCCACCTTCAGCGATGTGCTCATTTTCCTGGACCGTGAGCCAAACAGGCTGAGAGCTTT
CCAGGTCATCGAGACTCAGGATCCTCCACGTCGGCTGGCACTCACGCCTGCCCACCTGCTCTTCATTGCG
GACAATCATACAGAACCAGCAGCCCGCTTCCGGGCCACGTTTGCCAGCCACGTGCAGCCAGGCCAATATG
TGCTGGTAGCAGGGGTCCCAGGCCTCCAGCCTGCTCGGGTGGCAGCCATCTCCACCCACGTGGCCCTTGG
GTCCTATGCTCCTCTCACGAGGCACGGGACACTCGTGGTGGAGGATGTGGTAGCCTCCTGCTTCGCAGCT
GTGTCTGACCACCATCTGGCTCAGTTGGCCTTCTGGCCCCTGCGACTGTTTCCCAGCTTGCCATGGGGCA
GCTGGACCCCAAGTGAAGGTGTTCACTGGTACCCTCAGCTGCTCTACCGCCTGGGGCGTCTCTTGCTAGA
AGAGAGCACCTTCCATCCACTGGGCATGTCTGGGGCAGGAAGCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_053384
Insert Size 1236 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_053384.1, NP_445836.1
RefSeq Size 2272 bp
RefSeq ORF 1236 bp
Locus ID 84399
Gene Summary member of the hedgehog family of secreted morphogens; regulates chondrocyte (cartilage cell) differentiation [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...