Marf1 (NM_133421) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Lkap
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207516 representing NM_133421 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGGAACGGAACTGAGAACCCTTGCAGCAGAACACTTGGATGGTTACACCAAAATAACGATGCTA AGCCGTGGCTTTGGAAATTCTCTGGTTGCTTTTCTCTTCCTGAGCAGACATTGCCTCTTAGTTCCCAAAC GAAAGAGTACATGGAGAACAAGAAAGCTGCTGTGGAGTTAAAGGATATACCATCTCCCCTTCATGTTGGC TCTAAATTTTTCCCAGCAGTTCCCCTTCCTGATATTCATTCTCTTCAGCAACCTAAAGTACAGCTCTCAG CTATTCCCAAAGTAAGCTGCTGTGCTCATTGCCCTAATGAACCCTCTACTTCACCCATGCCTTTTGGTGG TGGCAGTGGCAGTAGTGGACGTTCTGGGAGCTTGATTCACACAGGCTCACTATTAGACTCACCAAGCACT GGGACAGTCACGTGTCAAGTAGGGTCAGATTTTGCTTTCCAGTCCGTGTCTTCATTGCAGAATGCCTCAG CTAGGAACAACTTGGTGGGCCTTGCGAGTGACCTCCCCAGCATGTGTGTAGACAGTAACCTACCTTCCTG CAAGCACCTGTCGTGTTGTGGGAAGTTCCATTTCCAGTCATGTCGCAGTAATGTACACAAGCTGCATCAG TTCCAGACTCTCCAGGGCTGTACCTCTGCTGGCTATTTCCCCTGCTCTGATTTCCCAAGTGAGGCTCCTG GGCATTTGGAAGAGCACCTTTCCCATTCAGAGCTTACACCTCACTTGTGCACCAACTCTCTCCACCTAAA TGTGGCACCTCCGGTTTGTTTAAAAGGCTCACTTTACTGTGAAGACTGTCTAAACAAGCCAGCAAGAAAT AGTATAATTGATGCTGCTAAAATCTGGCCAAATATACCACCTCCAAGTACTCAGCCTGCACCTCGAGCTG TTCCTCTGTGTAATCGCTGTGGAACAAAAGGGATGGAAAAGGAGACCTCGTTGTTGGCAACCAGTCTAGG CAAAGCTGCTTCTAAATTTGGGTCACCAGAAGTTGCAGTCACTGGACAGGTGCTGGAGACCCTCCCCCCC ATTGGTGTTTTCTGGGATATTGAAAACTGCTCAGTCCCCTCTGGCCGGTCAGCAACAACTGTTGTACAGA GAATCCGTGAGAAGTTTTTTAGAGGACACAGAGAAGCAGAGTTTATCTGTGTGTGTGACATCAGTAAAGA GAACAAGGAAGTCATTCAAGAGCTGAATAATTGTCAGGTAACTGTTGCTCACATAAACGCTACCGCAAAG AATGCTGCTGATGATAAACTCCGTCAGAGCCTGCGAAGGTTTGCAAATACGCACACTGCTCCAGCTACTG TGGTTCTTGTGTCAACTGATGTTAATTTTGCATTAGAACTCAGTGATCTCAGGCACCGGCATGGTTTCCA CATCATTTTGGTACATAAAAACCAGGCCTCTGAAGCCTTGTTACATCATGCTAACCAGCTGATCAGATTT GAAGAGTTCATTTCCGATCTACCCCCCAGGTTACCACTGAAGATTCCTTGCCACACTCTACTCTATGTTT ATAACCTGCCGGCAAATAAAGATGGCAAGAGCATCAGCAACAGGCTCAGGCGCCTCTCTGATAACTGTGG TGGCAAGGTGCTAAGTATCACAGGCTGCAGCGCTATTCTCCGCTTTATAAACCAAGACAGCGCAGAGCGG GCCCAGAAGCGAATGGAAAATGAAGATGTCTTTGGCAACAGGATCATTGTATCCTTTACTCCAAAGCATA GAGAATGCTTTGAAGCCAAGAGTTCGAATGCTGTTGTTGATAAAGCAAAGTCTCCCAAAAAAGTCAAGAA CACAAAACTGTGCCTCATCAAAGACACAGGTGAACAGTCACCCAGTGCCAAAGCCATGCCTGGGAAAGGG TTGCAGGCAAATTCTGGATCTGCTACAAAAAACACAAACTTTAAAGGTTTACAGGAGCTGTGCCGAATGG AACCAAAGTCTGGCAATAGAATCCGTGACCATCAGCAAGGCCATGGGAGCCTGGCCACACTGCCTAACAG TGGCCCAACCGCTTCAGTGCCCACACTGAAAAACACAGGTGTGAGGGAGACACTTCACAGAAGCAGTCAA AAAAAAGAGAACCTCAGTTCCCAGAGCATTGCCAGCTCTCCTGCACAGAAGAAAAAGAGAGAGGGGACTG TCTTCCAAATAAGCTACCCATCTGCATTCAGCCAGCTGATCGCCTCAAGGCAAGTCAGTCCTCTGTTCAG CTCTCAGTCTTGGTCTCCGAGGAGTATGTCTCCAAACCTTTTGAACACCGCCTCGCCACTTGCTTTCAAC ATTGCCAATCCCAGTAGCGCTGCTGACTGCTCAGACCCATTTGCAAATGGTGTTGACATCCAGGTCAGCA ATGTGGACTACAGGCTGTCCCGGAAGGAGCTACAGCAGCTCCTCCAAGAAGCCTTCTCCAAGCATGGCCA GGTGAAGAGTGTTGAGCTCAGTCCCCATACTGATTACCAGCTCAAAGCCATCGTGCAGATGAGAAACCTG CATGACGCAATCTGTGCTGTGAACAGCCTCCACAGATACAAAATTGGCAGCAAGAAGATACTGGTGTCAC TCTCCACAGGAGCTGCCAACAAGTCACTCTCTTTACTAAGTACAGAAACAATGTCTATTCTTCAGGATGC TCCTGCCTGTTGTTTGCCTCTCTTTAAATTTATAGACATCTATGAAAAGAAGTATGGACACAAGTTAAAT GTGTCTGATTTATATAAATTAACAGACATGATTGCAATCCGTGAACAGGGAAATGGGCGACTAGTGTGTC TCTTACCCAGTAACCAGGCCCGCCAGAGCCCACTGGGCTCTTCCCAGTCACATGATGGCTCCTCTACAAA CTGCAGCCCAGTTCTCTTTGAAGAGTTGGAGTATCACGAATCTGTGTGCAAGCAGCACTGCTCGAACAAG GACTTCAGCGAACTTGTATTTGATCCAGACTCTTACAAGGTTCCTTTTGTGGTTCTCTCTTTGAAAGTGT TCGCACCTCAAGTTCATAGTCTCCTGCAGACCCACGAGGGCACTGTGCCTTTGCTGAGCTTTCCAGATTG CTATGCTGCGGAGTTTGGTGACCTAGAAATTACGCAAGACAGAGACAAAGGTGTCCCCTTAGAGCACTTC ATCACCTGCATTCCAGGAGTGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTCACA ACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTTCGTTCTAAAAGCCCTGTGAGAAATCCTCAGCT GATTCAGTTCAGCAGAGAAGTCATTGATTTACTAAAGAACCAACCATCTTGCATACTACCAATCAGTAAT TTCATCCCTTTGTACCATCACCATTTTGGAAAGCAGTGCCGGGTTTCAGACTATGGATATTCCAAGCTGA TTGAGCTACTAGAAGCCGTGCCTCATGTGTTGCAGATTCTTGGAATGGGCTCCAAGCGTCTACTGACCCT CACTCATAGGGCACAGGTGAAGCGCTTTACTCAGGATTTACTGAAACTTTTCAAGTCCCAGGCCAGCAAG CAGGTCATTGTGAGCGATTTCTCACAGGCTTATCATTGGTGTTTCTCAAAGGACTGGGATGTCACGGAAT ACGGTGTTTGTGACTTGATTGATATAATATCAGAGATCCCAGACACAACTATTTGCTTGTCCCAACAAGA TGATGACATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGACAAAGCAGTTC TCCAAAGATGTTGTGGACTTGCTGCGCCACCAGCCCCATTTCCGAATGCCCTTTAACAAGTTCATTCCCT CTTACCACCACCACTTTGGCCGGCAGTGCAAACTTGCATATTATGGATTTACCAAGTTGCTGGAACTTTT GGAAGCCATACCTGAGATTTTACAAGTGTTGGAATGTGGAGAAGAGAAAATCCTGACTTTGACTGAAGTG GAGCGATTCAAAGCTCTAGCTGCCCAGTTTGTTAAGCTTTTGCGTTCCCAAAAGGGTAACTGCCTTATGA TGACAGATCTGTTCACAGAATATGCTAAAACTTTTGGTTATACATTTCGTCTCCAAGACTATGATGTCAG TTCAGTCTCAGCTTTGACACAGAAGCTCTGCCATGTTGTAAAGGTTGCTGATATGGAGTCTGGCAAACAA ATCCAACTGATAACCCGGAAGTCTTTGCGGCCTCTCACTGTTCAGTTGCTGGTGGTGTTGATGTCTTGGG AAGGAGATGCTTATCTTCCCGTTGATGAGCTCAAGAGACATTATGAAACCACTCACAGTACTCCTCTCAA CCCCTGTGAATATGGTTTCATGACTTTGACTGAGCTACTGAAGAGTCTGCCTTCTTTAGTAGAGGTTTTT ATGAATGATAAGGCAGAAGAGTGTGTGAAGCTCACAAGCCTATACTTGTTTGCAAAGAATGTGAGGTCTT TGCTTCATACCTATCACTACCAGCAGATTTTTCTTCATGAATTTGCCATGGCCTATACTAAATATGTAGG AGAAACACTGCAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAGCTACTGGGAGCCATCCCACAGGTG GTCTGGATAAAAGGACATGGTCATAAGAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGGCTAAGTT CATTTGACCTTTCCCCTGTCAATCATGAAGACCAGCCTGCAGTCAGCAGGCAGATCCTGGAGGTGCCAGA ACCCCATCTAGCCTCAGAGCTCCATCTCAGAACAGGACCCAGCCAGATGGAGCAGGAACTTCTGCAATTG GCCAACAGCTCCCCTGTTGACCTTCTCTGTGCTCCTGTTCCTTCATGCCTGCCATCCCCTCAGCTAAGAC CAGATCCTGTCATCCTGCAGCCCACTGATCTCATTCAGTTTGAGGAACACCCTCAGGAGCCTTTGGGAGT TTTATTTTTAAACCAAGAAGAAAAAACAGAAATTCCAATACCAGTAAAGAAGGGGAACCTGTCCTGTAAC TCCTCACCCAGTAGCCCAGCATCTGCTTCTGCTCCTCCCAAACCCTCCTCAGAAGCCCCCGGGCTACTGA TTAGCAAGGATGCAGTGGAAAGCCCAGCAAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCAACTT TTCTTTTGCACCTGTGACCAAGCTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_133421
Insert Size	5208 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_133421.2, NP_596912.1
RefSeq Size	7617 bp
RefSeq ORF	5208 bp
Locus ID	170946
UniProt ID	Q8VIG2
Gene Summary	protein of unknown function, may associate with LIM kinase-2 [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.