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Pif1 (NM_001044253) Rat Untagged Clone
CAT＃: RN207530
Pif1 (untagged ORF) - Rat Pif1/Rrm3 DNA-helicase-like protein (Pif1), (10 ug)
CNY 8,270.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN207530 representing NM_001044253
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTTCTAGCACTGAGGTGGCGACCGATGAATGTGATGACACCGAGCTGCGGTGCCGGGTAGCCGTAG AGGAGCTGAGTCCTGGAGGGCAACCTCGCAAGCGCCAGGCTCTGCGCGCCGCAGAGCTGAGCCTAGGTCG GAATGAACGCCGCGAGTTGATGCTGCGACTGCAGGCACCCGGACCCGAAGGGCGGCCACGCTGTTTCCCA CTGCGCGCCGTGCGCCTCTTCACCCGCTTCGCCGCAGTTGGGCGCAGCACTTTGCGACTCCCGGCCGATG GCGTGCCCCGGGCTGGCTCAGTGCAACTGCTGCTCTCTGACTGTCCCCCGGAGCGCTTGCGCCGCTTCCT GCGCACCCTGCGCCTGAAGCTGGCGGTCGCCCCTGGGCCGGGACCCGCCTCTGCCCGTGCGCAATTGCTC GGTCCGCGGCCCCGAGACTTTGTCACGATCAGTCCAGTGCAGCCGGAGGAACTGCGCCGTGCTGCGGCCA CCAAGGCTCCAGACTCAGCGCTGGAAAAGCGGCCAATGGAATCCCAGCCTAGTATGGAAGCTCCAAGGTG GCCCCTGCCTGTGAAGAAGCTACGCATGCCCTCCAGCAAACCGAAGCTTTCCGAAGAGCAGGCCGCTGTG CTGCGGATGGTTCTGAAAGGCCAGAGCATATTCTTTACCGGGAGTGCAGGGACAGGGAAGTCCTACCTGC TGAAACATATCCTGGGTTCCCTGCCTCCTACTGGCACTGTGGCCACTGCCAGCACTGGGGTGGCAGCCTG CCACATTGGGGGCACCACCCTTCATGCCTTTGCAGGCATCGGTTCAGGCCAGGCTCCCCTGGCCCAGTGT GTGGCTCTGGCCCATCGGCCTGGTGTGCGGCAGGGCTGGCTGAACTGCCAGCGTTTGGTCATTGATGAGA TCTCCATGGTAGAGGCAGACTTCTTTGACAAGTTGGAAGCTGTGGCCAGAGCTGTCCGGCAACAGAAGAA ACCATTTGGAGGGATCCAGCTTATTATCTGTGGGGACTTCCTACAGTTGCCGCCAGTGACCAAAGGTTCC CAGCATCCTCGATTCTGCTTTCAGGCCAAGAGCTGGAGGAAGTGTGTACCAGTGACTCTGGAGCTGACTG AGGTGTGGAGGCAAGCAGACCAGACCTTCATCTCTCTCCTGAAGGCTGTGAGATTAGGCAGATGTTCAGA TGAAGTGACCCGCCAGCTCAGGGCCACAGCTGCCCATAAGGTGGGACGAGATGGAATTATAGCCACGAGA CTGTGCACCCATCAGGATGATGTGGCCCTTACCAATGAGAAGCGGCTGAAGGAACTACCAGGTGATGTAC ACAGCTTTGAGGCTATAGACAGTGACCCTGAGCTAAGCCGGACCCTGGATGCTCAGTGCCCTGTAGGCCG TGTCCTTCAGTTAAAGCTGGGGGCTCAAGTCATGCTGGTGAAGAACTTAGCAGTGTCTCGGGGCCTGGTG AATGGTGCCCGAGGGGTGGTAGTCGGGTTTGAGTCAGAAGGAAGAGGGCTTCCCCGGGTACGGTTCCTGT GTGGCATCACTGAGGTCATCCGCACCGACCGCTGGACAGTACAGGTCACTGGGGGCCAGTACCTCAGCCG GCAGCAGCTTCCCCTACAGCTAGCCTGGGCAATGTCCATCCACAAAAGCCAGGGCATGTCTCTGGACTGT GTAGAGATCTCTCTGGGCCGTGTGTTCGCCAGTGGTCAAGCCTATGTGGCCCTCTCCCGGGCCCGTAGCC TCCAGGGTCTACGTGTGCTAGACTTCGACCCCACTGTGGTTCGATGCGACTCCCGAGTGCTGCAATTCTA TGCCACCCTGCGGCAGGGCAGGGGCCTCAGTCTGGAGTCGCAAGACGATGAGGAGGCAAGCTCAGATCTG GAGAACATGGACCCAAACCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001044253 |
| Insert Size | 1914 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001044253.1, NP_001037718.1 |
| RefSeq Size | 1915 bp |
| RefSeq ORF | 1914 bp |
| Locus ID | 367645 |
| UniProt ID | Q1HG60 |
| Gene Summary | DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR207530 | Pif1 (Myc-DDK-tagged ORF) - Rat Pif1/Rrm3 DNA-helicase-like protein (Pif1), (10 ug) |
CNY 8,270.00 |
|
| RR207530L3 | Lenti ORF clone of Pif1 (Myc-DDK-tagged ORF) - Rat Pif1/Rrm3 DNA-helicase-like protein (Pif1), (10 ug) |
CNY 9,980.00 |
|
| RR207530L4 | Lenti ORF clone of Pif1 (mGFP-tagged ORF) - Rat Pif1/Rrm3 DNA-helicase-like protein (Pif1), (10 ug) |
CNY 10,930.00 |
