Recql4 (NM_001130494) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207699 representing NM_001130494 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGCTTGCGACCGTTCGCGCGTGGCTGCAGGATTGGGAACGCGCTTTTGTACGGCTGCACGGGC GACGGCCAGCAAAGGAGGATGTGGAGGCGGCACCCGAGGAGACCCGCGCGCTCTACCGTGAGTACCGTAA CCTAAAGCAGGCAGTGAGTCAGGCCGACGACGGACACCGCGTCCAAAAGCAATCACTTGCCAAGGCAGCT GAGGAGGAACAGGAGCCAAGCTGCTGGGGTTCCCACCTGAACCGAGCTGCAACCCAGAACACACAGTCTA TTCCAAAGCAGAGCCCACTGAGTTCTATACAAGACTATGGGAAGAGGCTCAAAGCCAATCTTAAAAACAC ACTACAGGGCGGGCCAACCCTGAGTCGAAAACTCCAGCATCAGAAGAGATCCTTGTCCATAGTGCCCACA TCAAGGCCACCAGGCCCAAGGACTGAATCCCCCTGTCCAGAACAAGCTAATGATGCTCTTCCCCAGGTTC CTGTGCCCCAGCCAAGGCAAGGCCAACTCCAGAAGCTCCAGTCATCCCTGAGCCTGAGGTTGAGTTCCCT AGACCCTGGTTGGTTAGAGAGGTGTCAAAACAGAGTTTCAGATCCTCTAGAGGTTCCAGGTGCTTGTGGG CTTGACCTGAGTGCAAAGGAGTCACAACCTCAGATGTCAGGCAAGGTGAATGTCATTAGTCCTGACATCC TTTCAGAAGTATCTTCACAGAGCCCACAGGCCCTAGCCCAACAGCCAGCCCAAGTTTTGTCACAGATCCC CAAATCCAGCAACAGTAAAGACAGGAAGCGGAAACGGAATGAGAAGGGGGAGGACTTTGCACAAGCCCAG CCCAGCAGCCAAGCAGGACCCCTGTCTGAGGGAGCTGGGGCTACAGCACATGGGCAAGGCCCTCCAGGAG AACCCACACAAGTGACTGTCCCTCAGCACCGCTGCTCTTCAAACCAGGCCAGGACAGAGAATGCTAAGAG TGCGGCCCACCTCCCCATGTCCCCTCGGCCAGCTTCCCTAGACAGAGGGAACTATGTTCGACTTAACATG AAAACCAAATGCTTTGTACGAGTTGGGGCCAGTCGGGGCAGGCTTCTCCGAAAGCAGGTATGGAAGCAAA AGTGGAAGAAGAAACAAGAGTGGTTTGGGAAAAGTGGACCCAGGGCCACAGTCAAGGACACTTGTTTCCG GTGTGGGCAGTTTGGTCACTGGGCATCCCAGTGTTCCCAACCAGGCTCCACCCTGACCATCCAGCCAAAA GGTGACAGGAATGACAAACATCCCATTTCCACGTTGGGGGGAGTAGCACAGAAGACTGGCACCACTTCCT GTAACCACTCTGGTGAGGAAACTGAACCTGCTGGACCAGTGCTACCGGTGCCTCATTGCCCCACGCCAGT GTTACCCCTCTACCCACCAGGGCCCTTGGGACAGGTAGCAGACACCCCTGCTGAGGTATTCCAAGTCCTG GAGCAGCTAGGGCACCACACCTTCCGCCCTGGGCAAGAGCGTGCTGTCATGAGGATTCTTTCTGGCATCT CCACACTGTTAGTGTTGCCCACGGGTGCTGGCAAGTCTCTGTGCTACCAGCTTCCTGCACTGCTCTATGC CAAGCGAAGCCCCTGTCTCACACTGGTCGTCTCTCCTCTCCTGTCACTCATGGATGACCAGGTGTCTGAT CTGCCTTCATGTCTGAAGGCGGCCTGCCTCCACTCAGGAATGACCAAGAAACAACGAGAATCTGTCTTGA AGAAGGTACGGGCAGCCCAGGTGCATGTGCTGATTGTGTCCCCAGAGGCCTTGGTGGGATGCGGGGCTAA GGGTCCTGCCAACCTCCCCCCGGCCGCTCAGCTGCCTCCAGTCGCCTTTGCCTGCATTGATGAGGTCCAC TGCCTCTCTCAGTGGTCACATAACTTCCGGCCCTGCTACTTACGTGTTTGTAAAGTACTTCGGGAGCATA TGGGTGTGCGCTGCTTTTTGGGTCTCACAGCCACAGCCACACGAAGCACTGCTCGTGATGTGGCCCAGCA CCTGGGCATAGCTGAAGAGCTTGAGGTCAGTGGGTCAGCCAGCATCCCTGCCAATCTGCACCTCTCCGTG TCCATGGATCGAGACTCAGACCAGGCTCTGGTGACACTGCTACAAGGTGACCGCTTCCGTACCCTGGATT CGATTATCATTTACTGTGCTCGACGAAAGGACACAGAGCGGGTGGCTGCCCTCCTCCGGACCTGCCTGTC CACAGTGAGAGATTCAAAGCCAAGAGGCCGTGGCCCTGAGACTTTAGCGGAAGCATACCATGCTGGCATG TGTAGCCAGGAACGGAAACGAGTACAACAGGCCTTCATGCAGGGCCACCTGCGCATGGTAGTGGCCACAG TGGCATTTGGGATGGGACTGGACCGTCCAGATGTTCGGGCTGTGCTACACCTGGGACTGCCTCCAAGCTT CGAGAGCTACGTGCAAGCTATTGGCCGTGCAGGACGTGATGGGAAGCCTGCCCATTGCCACCTGTTCCTG CATCCTCAGGGTGAAGACCTCTGGGAACTGAGCAGACATGTCCATGCTGATAGCACTGACTTCCTGGCTG TGAAGAAGCTGGTACAGCGTGTGTTCCCAGCCTGCTCATGCGCCCAGAGGCCTATGTCCCCACTTGAGGA AGTCAAAGAGCACAGTGGCCAACAAACATACCCTGTACTGGGACTAGCCTGCCGGGGCCATAAGCGGGCA CTCCCAGTGCAGTCTACAGTACTGGCTCTGGACATGAAAGAGGAGGCCATTGAGACTTTGCTGTGCTATT TGGAACTACACCCTCGGCACTGGTTGGAGCTGCTGCCATACACCTATGCCCACTGCCATCTGCGTTGCCC TGGGGGCAGTGCCCAGCTGCAAGCTCTGGTCCACAGGTGTCCCCCTTTGGCTGCATGCTGGGCCAAGTGG CCACCTAAAGACACAAGTCAGGGTAGCAGCGCCTTAGAGTTTGATGTGGTGGAACTGGCAGACTCCATGG GCTGGGAGTTGGCCTCTGTACGGCAGGCTCTCCACCAGCTGAAGTGGGACCCAGAGCCAAAGAAAGGTGC ACCACAGGGCACAGGAGTGCTTGTGGAGTTCAGTGAGTTGGCCTTTCACCTGCACAGTCGTGGGGACCTG ACAGATGAGGAGAAGGACCAGATCTGTGACTTTCTGTACAGCCGTGTGCAGGCTCGTGAACACAAGGCCC TGGCCCACCTACACCAAATGTCCAAGGCCTTTCAAAGTGTAGCATTTCCCAGTTGTGGACCCTGTTTAGA GCAGTCTGACGAGGGGCACAGCAATCGGGTGAAGACCCTGGTCAGCTACTACTTTGAGGAGGAGGAGGAG GAGGAAGAGGAAGAAACTATGAAGGATACTCAGGATCCAAAACCTGGGCAGACCCAGCTTCAGGATTGGG AGGACCAAGTTCGCCGCGATGTCCACCAGCTCCTGTCCCTGAGGCCAGAAGAAAAGTTTTCAGGAAGGGC TGTGGCCCGCATCTTCCATGGAATTGCAAGTCCATGCTACCCAGCCCAGGTGTTTGGGCAGGACCGGCGC TTCTGGAGAAAGTACCTACACCTGGACTTCCACGCCCTCATGCACCTAGCCACAGAGGAGCTCCTGCTCA GAGGCCGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001130494
Insert Size	3651 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001130494.1, NP_001123966.1
RefSeq Size	3860 bp
RefSeq ORF	3651 bp
Locus ID	300057

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