Usp32 (NM_001107032) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN207779 representing NM_001107032 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCAGCACTGCTTCATCAGGGAAGTGCTTGGGGATGGAGTCCCTCCTAAAGTTGCTGAGGGTGAAA AGGTAAACTATGAAAAATTCAGAAATTGGCTTCTTCTCAACAAAGATGCTTTTACCTTCTCTCGATGGCT GTTGTCTGGGGGTGTATATGTGACCCTCACTGATGACAGTGACACTCCTACTTTCTACCAAACTTTGGCT GGAGTCACACATTTGGAAGAGTCAGACATCATTGATCTGGAGAAACGCTATTGGCTACTGAAGGCCCAAT CCCGTACTGGACGTTTTGACTTAGAGACCTTTGGTCCATTGGTTTCACCACCTATTCGCCCTTCTCTGAG TGAAGTTTGTTTCAAGGTGTTTGATATTGACCGTGATGGAGTTCTCTCCAGGGTTGAACTGAAAGACATG GTGGTTGCTCTTTTAGAGGTCTGGAAAGATAACCGCACTGATGATATTCCTATGGGCCATCTTACTCTCG AAGACTATCAGATATGGAGTGTGAAAAATGTTCTTGCCAATGAGTTTTTGAATCTCCTCTTCCAGGTATG CCACATAGTTCTGGGGTTAAGACCAGCTACTCCAGAAGAAGAAGGACAAATTATTAGAGGATGGTTAGAA CGAGAAAGCAGGTATGGTCTGCAGCCAGGACACAATTGGTTTATCATCTCCATGCAGTGGTGGCAACAGT GGAAAGAATATGTCAAATACGATGCCAACCCTGTTGTAATTGAGCCATCATCTGTTCTAAATGGAGGAAA ATTTTCATTTGGAACAACAGCACATCCTATAGAACATGGAGAAGATAGAATCAGTAACAACCTTAGTTAT ATGAATACCACAGAAGAAAAATACTCAGACAACATTTCTTCTGCATCTGAAGCCTCAGAGTCTACTGGCA GTGGATTTCTGTATTCTGCCACACCAGGGGCAGATATGTGCTTTGCAAGGCAACATAACACTTCTGACAA TAACAACCAGTGTTTGCTAGGAGCCAATGGGAATATCCTTTTGCACCTTAATCCTCAGAAGCCAGGAGCT ATCGATAACCAACCACTAGTAACTCAGGAACCAGTAAAGGCTACATCATTAACACTAGAAGGAGGACGAT TAAAACGAACTCCACAGCTAATCCATGGAAGAGATTATGAAATGGTCCCAGAACCTGTGTGGAGAGCACT TTATCATTGGTATGGATCAAACCTGGCCCTCCCTAGACCAGTAATCAAGAATAGCAAAACGGACATCCCA GAACTGGAGTTATTTCCTCGCTATCTTCTCTTCCTTCGACAGCAGCCTGCCACACGGACACAGCAGTCTA ACATCTGGGTGAATATGGGAAATGTACCCTCTCCAAATGCGCCTCTAAAGCGGGTGTTAGCCTATACAGG CTGTTTTAGTAGAATGCAAACCATCAAGGAAATTCATGAATATCTGTCTCAAAGGCTTCGTATTAAAGAG GAAGATATGCGACTGTGGCTATACAATAGTGAGAACTACCTGACTCTTCTGGATGATGAGGATCATAGGT TGGAATATTTGAAAATCCAGGATGAGCAGCACCTGGTAATTGAAGTTCGCAACAAAGATATGAGTTGGCC TGAAGAAATGTCCTTTATAGCAAACAGTAGCAAAATAGATAGACATAAAGTTCCCACAGAAAAGGGCGCC ACAGGTCTGAGTAACCTGGGAAACACATGCTTCATGAACTCCAGCATCCAGTGTGTTAGTAACACACAAC CACTGACACAGTACTTTATCTCAGGGAGACATCTTTATGAACTCAACAGGACAAATCCCATTGGTATGAA AGGCCATATGGCTAAATGCTATGGAGATTTGGTGCAGGAACTTTGGAGTGGAACTCAGAAAAATGTTGCC CCCTTAAAGCTTCGGTGGACCATAGCAAAATACGCTCCCAGGTTTAATGGGTTCCAGCAGCAAGACTCCC AAGAACTTCTGGCTTTCCTCTTGGATGGTCTCCATGAAGATCTCAACCGAGTCCACGAGAAACCATATGT GGAACTAAAGGACAGTGATGGCCGACCTGACTGGGAAGTGGCTGCAGAGGCCTGGGACAACCATCTAAGG AGAAATAGATCAATTGTTGTTGACTTGTTCCATGGTCAGCTAAGATCCCAAGTCAAATGCAAGACATGTG GGCATATAAGTGTTCGATTCGACCCTTTTAATTTTTTGTCTTTACCACTACCAATGGACAGTTACATGCA TTTAGAAATAACAGTAATTAAATTAGATGGTACCACCCCCATACGATATGGACTAAGATTGAATATGGAT GAAAAGTACACAGGTTTGAAAAAGCAGCTGAGTGATCTCTGTGGACTTAAATCAGAACAAATCCTTTTTG CAGAAGTGCATAGTTCTAACATAAAGAACTTCCCTCAGGACAACCAAAAAGTTCGACTCTCAGTGAGTGG ATTTTTGTGTGCATTTGAAATTCCTATTCCTGCATCTCCAGTTTCAGCTTGCAGTCCGATTCAAACAGAT TGCTCCTCTTCTCCATCTACAAATGGACTGTTCACCGTGACTACCAATGGGGATCTGCCTCGACCTATAT TCATCCCCAATGGAATGCCAAATACAGTTGTACCATGTGGAACTGAGAAGAATGCCACCAACGGAATAGT GAATGGTCATATGCCACCTCTTCCTGATGACCCCTTTACAGGCTACATCATTGCAGTCCACCGAAAAATG ATGAGGACAGAACTGTACTTCCTGTCATCTCAGAAGAATCGCCCCAGTCTCTTTGGAATGCCCCTGATTG TTCCATGCACTGTGCATACCCGGAAGAAAGATCTCTATGATGCAGTTTGGATTCAAGTATCTCGATTGGC CAGTCCACTCCCACCTCAGGAAGCCAGCAATCATGCACAGGATTGTGATGACAGCATGGGCTATCAATAT CCATTCACTCTCCGGGTTGTACAGAAAGATGGAAATTCCTGTGCCTGGTGTCCATGGTATAGATTTTGCA GAGGCTGTAAAATCGATTGTGGAGAAGACAGAGCTTTCATTGGAAATGCCTGTATTGCTGTGGATTGGGA CCCTACAGCCCTTCACCTCCGCTATCAAACATCACAGGAAAGAGTTGTAGAGGAGCATGAAAGCGTGGAG CAGAGTCGGCGAGCACAAGCTGAGCCCATCAACCTGGACAGCTGTCTCCGTGCCTTCACCAGTGAGGAGG AACTGGGGGAAAATGAGATGTACTACTGCTCCAAGTGTAAAACTCACTGTTTGGCAACCAAGAAGCTGGA CCTCTGGAGGCTTCCTCCAATCCTGATTATTCACCTTAAACGATTTCAGTTTGTAAATGGACGGTGGATA AAATCACAGAAAATTGTTAAATTTCCTCGTGAAAGTTTTGACCCTAGTGCTTTTCTAGTACCAAGAGACC CAACTCTTTGCCAGCATAAACCACTCACACCCCAGGGTGATGACGTCTCTGAGCTACCGATCCCAGCAGG AGATGTGAAGAAAGTGGATGTCCAGAATCCAGCAGGGGAGGAGGATGTACTCCTGAGCAAGAGCCCGTCT TCACTCAGTGCAAATATCACCAGTAGCCCAAAAGGTTCACCTTCTTCATCAAGAAAAAGTGGAGCCAGCT GTCCCTCCAGCAAAAATAGCAGCCCTAACAGCAGCCCACGGACCCTGGGGAGAAATAAAGGGAGGCTTCG GCTGCCTCAAATTGGAAGCAAAAATAAATTGTCCAGTAGTAAGGAGAATTTGGATGCCAGCAGAGAGAAT GGGGCTGGGCAGATCTGTGAACTGGCTGACACCTTGAACCGAGGACATGTGTTGGGAGGCAGCCAGCCTG AGCTGGTCACTCCTCTAGATCATGAGATCACTTTAGCTAATGGATTTCTTTATGAGCATGAGACATGTGG CAATGGCTATAGCAATGGTCAGCTTGGAAACCATAGTGAAGAAGACAGCACTGATGACCAAAGAGAAGAT ACACACAGTAAACCTATTTATAATCTATATGCAATTTCGTGCCATTCAGGAATTCTGGGTGGGGGCCATT ACGTCACTTATGCCAAAAATCCAAACTGCAAGTGGTACTGTTACAATGACAGCAGCTGTAAGGAACTTCA CCCCGATGAGATAGACACCGACTCTGCCTACATCCTTTTCTATGAGCAGCAGGGGATAGACTGTGCACAA TTTCTGCCAAAGATAGATGGCAAGAAGATGGCAGACACAAGCAGCATGGATGAAGACTTTGAATCTGACT ACAAGAAGTACTGTGTGCTACAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107032
Insert Size	4296 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107032.1, NP_001100502.1
RefSeq Size	6396 bp
RefSeq ORF	4296 bp
Locus ID	303394

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