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Sacm1l (NM_053798) Rat Untagged Clone
CAT＃: RN208191
Sacm1l (untagged ORF) - Rat SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), (10 ug)
CNY 7,510.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Synonyms | Sac1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN208191 representing NM_053798
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCACAGCCTACGAGCATCTGAAGCTGCATATTACACCTGAAAAGTTTTATGTGGAAGCTTGTG ATGATGGAGCAGATGATGTACTCATCATTGACCGCGTGTCTACAGAGGTCACCCTTGCAGTCAAAAAAGA TGTTCCCCCTTCTGCTGTTACAAGACCAATATATGGGATAATGGGCACAATCCATCTGGTGGCAGGCAAT TATCTTGTTGTCATTACCAAAAAGATGAAAGTAGGTGAATTTTTTAATCATGTAATCTGGAAAGCCACAG ATTTTGATGTTCTTTCTTATAAGAAGACAATGTTACACTTAACTGATATTCAGTTACAAGATAATAAAAC TTTCCTAGCAATGCTAAACCATGTCTTGAGTACGGATGGATTTTACTTTTCAACAACATATGACTTGACC CATACTTTGCAACGGCTATCTAACACGAGTCCAGAATTCCAAGAAATGAGTCTCCTAGAAAGGGCAGATC AGCGGTTTGTATGGAACGGGCATCTGCTCAGAGAACTGTCTGCTCAGCCAGAGGTCCATCGGTTTGCTCT TCCTGTGTTGCATGGCTTTATTACTATGCATTCATGTTCTATTAACGGGAAATATTTTGATTGGATTCTC ATCTCCCGGAGGAGTTGTTTCAGAGCCGGTGTGCGTTATTATGTGAGAGGAATTGATTCTGAAGGCCACG CAGCTAACTTTGTAGAAACAGAACAGATTGTGCACTACAGTGGGAACAGGGCTTCATTTGTACAGACTCG AGGATCAATACCTGTTTTCTGGTCACAAAGACCAAATCTAAAGTACAAACCAGATCCACAGATCAACAAA GTAGCAAATCACATGGATGGTTTCCAAAGGCATTTTGATTCACAAGTAATTATTTATGGAAAACAAGTAA TAATTAATCTGGTGAACCATAAGGGATCAGAGAAACCACTTGAGCAAACATTTGCAAAAATGGTGTCATC CTTGGGAAGTGGGATGATCAGATATATTGCCTTTGACTTCCATAAGGAGTGTAAAAATATGAGATGGGAT CGACTGAGTATTTTATTGGATCAAGTAGCAGAAATGCAGGATGAATTAAGTTATTTTTTAGTAGACTCTG CTGGCAAGGTGGTGACAAACCAGGAAGGAGTTTTCCGCAGCAACTGTATGGATTGTCTAGACAGAACGAA TGTGATACAGAGTTTATTAGCTCGGCGTTCCCTTCAGGCACAGCTGCAGAGACTAGGAGTTTTGCATGTG GGACAAAAGCTTGAAGAACAAGATGAGTTTGAGAAGATTTACAAAAATGCCTGGGCCGATAATGCTAATG CTTGTGCCAAACAGTATGCAGGAACCGGTGCCTTGAAGACTGACTTTACAAGAACTGGAAAAAGAACTCA GTTGGGACTGGTAATGGATGGCTTCAACTCATTATTACGGTACTACAAGAACAACTTTTCTGATGGATTT AGACAAGATTCTATAGACTTATTTCTTGGAAACTACTCAGTGGATGAATTAGACTCTCACAGTCCTTTAA GTGTTCCAAGGGACTGGAAATTCCTGGCGTTGCCTATCATCATGGTTGTTGCCTTTTCAATGTGCATCAT CTGTTTGCTTATGGCTGGTGACACTTGGACAGAAACACTGGCATATGTCCTCTTCTGGGGAGTTGCAAGC ATTGGAACATTTTTTATTATTCTTTACAATGGCAAAGATTTTGTTGATGCTCCCAGACTTGTACAGAAAG AAAAGATAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_053798 |
| Insert Size | 1764 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053798.2, NP_446250.1 |
| RefSeq Size | 3370 bp |
| RefSeq ORF | 1764 bp |
| Locus ID | 116482 |
| UniProt ID | Q9ES21 |
| Gene Summary | This gene encodes an integral membrane protein, which is localized to the endoplasmic reticulum, and functions as a phosphoinositide phosphatase that hydrolyzes phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, and phosphatidylinositol 3,5-bisphosphate. Deletion of this gene in mouse results in preimplantation lethality. Other studies suggest that this gene is also involved in the organization of golgi membranes and mitotic spindles. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Dec 2017] Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (1). COMPLETENESS: complete on the 3' end. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR208191 | Sacm1l (Myc-DDK-tagged ORF) - Rat SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), (10 ug) |
CNY 4,368.00 |
|
| RR208191L3 | Lenti ORF clone of Sacm1l (Myc-DDK-tagged ORF) - Rat SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), (10 ug) |
CNY 9,410.00 |
|
| RR208191L4 | Lenti ORF clone of Sacm1l (mGFP-tagged ORF) - Rat SAC1 suppressor of actin mutations 1-like (yeast) (Sacm1l), (10 ug) |
CNY 10,260.00 |
