Gli2 (NM_001107169) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208592 representing NM_001107169 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACACCAGGAGGGAAGGTACCATTATGACCCTCACCCTGTCCACGGTGTGCACGGGCCTCCCACGC TAAGTGGCAGTCCTGTCATCTCAGATATCTCCTTGATACGACTTTCTCCACACCCTGCTGGCCCTGGAGA ATCGCCCTTCAGCGCCCACCACCCCTACATGAACCCCCACATGGAGCACTACCTCCGGTCTGTGCACAGC AGCCCCACACTCTCAATGATCTCTGCCGCCAGGGGCCTCAGCCCTGCTGATGTGGCCCATGAACATCTGA AAGAGAGGGGACTATTTAGTCTCGCTGCCCCAGGCACCAACCCTTCAGACTATTACCACCAGATGACCCT CATGGCAAGCCACCCCACACCCTACGGGGACATTCTAATGCAAAGTGGCGGTGCTGCTAGCGCACCCCAT CTCCATGACTACCTCAACCCTGTGGATGCATCACGATTCTCCAGCCCACGTGTGACCCCGCGACTGAGCC GCAAGCGGGCTCTATCCATCTCCCCGCTCTCAGATGCCAGCCTTGACCTACAGCGCATGATCCGGACCTC TCCCAACTCACTGGTAGCCTACATCAACAACTCCAGGAGCAGCTCAGCAGCCAGCGGTTCTTACGGGCAT CTGTCAGCTGGTGCCCTCAGCCCAGCCTTCACCTTCCCCCACCCCATCAATCCCGTGGCGTACCAGCAGA TCCTGAGCCAGCAGCGGGGCCTGGGTTCAGCCTTTGGACATACACCACCCCTGATCCAGCCTTCACCCAC CTTCTTGGCTCAGCAGCCCATGACTCTCACCTCCATCACCGTGCCTACCCAGCTAGGCAGCAATAGCAGC AACTGTATAAGTGATGCCAACCAGAATAAGCAGAACAGCGAGTCGGCCGTGAGCAGCACCGTGAACCCCA TCACCATCCATAAGCGGAGCAAGGTCAAGACTGAGGCTGAGGGCCTACGGCCAGCCTCCCCGCTTGGACT GACACAGGAGCAACTGGCTGATCTCAAGGAAGATCTGGACAGGGATGACTGTAAGCAGGAGGCTGAAGTG GTCATCTACGAGACCAACTGCCACTGGGCAGACTGCACGAAGGAATATGACACCCAGGAGCAGCTGGTGC ATCACATCAACAATGAGCACATCCACGGGGAGAAGAAGGAGTTCGTGTGCCGCTGGCAGGCCTGCACGAG AGAGCAGAAGCCCTTCAAGGCCCAGTACATGCTGGTTGTCCACATGCGCAGGCACACGGGTGAGAAGCCG CACAAGTGCACGTTTGAAGGCTGCTCAAAGGCCTACTCTCGCCTGGAGAACTTGAAGACACACCTGCGGT CCCACACAGGGGAGAAGCCCTATGTGTGTGAACACGAAGGCTGCAACAAAGCCTTCTCCAATGCCTCGGA CCGTGCCAAGCACCAGAACCGCACTCACTCCAATGAGAAACCCTACATCTGTAAGATCCCAGGCTGCACT AAGAGGTACACAGACCCCAGCTCACTCCGCAAGCATGTGAAGACCGTCCACGGGCCAGATGCCCATGTGA CCAAGAAACAGCGTAATGATGTGCACCTCCGTGCTCCACTGCTCAAGGAGAATGGGGACAATGAGGCCGG TGCTGAGCCAGGTGGCCGGGGACCCGAGGAGAGTGTGGAGGCCAGTAGCACCAGTCACACTGCGGAGGAC TGCCTGCATATCAAAGCCATCAAGACAGAGAGCTCCGGGCTGTGCCAGTCCAGCCCCGGGGCCCAGTCAT CCTGCAGCAGCGAGCCCTCTCCCCTGGGCAGTGTCCCCAACAATGACAGTGGCGTGGAGATGCCGGGGAC AGGGCCCGGGAGTCTGGGAGACCTGACAGCACTGGACGACACGTCTCCAGGAGCTGACACATCAGCCCTG GCTGTACCTTCCACTGGTGGCCTGCAGCTGCGCAAACACATGACCACCATGCATCGCTTTGAGCAGCTGA AGAGAGAGAAGCTCAAGTCTCTAAAGGATTCCTGCTCGTGGGCTGGCCCAGCCCCACACACCCGCAACAC CAAGCTGCCTCCCCTTCCAGTGAATGGTTCTGTTCTGGAAAACTTCAACAATACTGGGGGCTGTGGACCA GCGGGACTGCTGCCCAACCAGCGGCTACCAGAACTGACAGAAGTGACCATGCTGAGCCAGCTGCAGGAAC GGAGAGACAGCTCCACCAGCACTATGAGCTCAGCCTACACCGTGAGCCGCCGCTCCTCCGGCATCTCCCC ATACTTCTCCAGCCGCCGTTCCAGCGAGGCCTCGCCTCTTGGTGGCATACGCCCGCACAATGCCAGCTCA GCGGACTCCTACGACCCCATCTCCACAGACGCCTCTCGGCGCTCCAGTGAGGCCAGTCAGTGCAGCGGTG GTGGCCCCGGGCTGCTCAACCTGACACCCGCACAGCAGTATAGCCTGCGTGCCAAGTACGCAGCGGCCAC AGGCGGGCCACCACCCACGCCACTGCCGGGCCTCGACCGCGTCAGCCTTCGTACCCGCCTGGCATTACTA GATGCTCCTGAGCGTGCACTGCCAGGCGCCTGCCCACAACCTCTGGGGCCACGGCGTGGTAGCGACGGGC CTACCTATAACCACGGCCATGGCTATGTAGGTGCGGCTCCTGCCTTACCCCATGAGGGGCCTAACGGCAG CACACGGCGGGCCAGCGACCCTGTGCGGCGACCTGACCCCCTTGTTCTGCCTCGAGTGCAACGTTTCCAC AGCATCCACAACATGAATCCAGGTCCGCTGCCCCCCTGCACTGATCGGCGTGGCCTGCATTTACAGAGCC ACCCCAGCGTAGATGGCAGCCTGACCCGCCATGCCTACTCTCCGCGACCCCCTAGCATCAATGAGAACGT GGTGATGGAAGCCGTGGCTGCTGGGGTAGAGGGTGCCGGGCTAGAGTCTGACCTGGGGCTGGTGGAGGAT GACCTCGTGCTGCCAGATGATGTGGTACAGTACATCAAGGCTCACACAGGTGGTGCCTTGGATGACAGCA CACGGCAGGTGTATCCCACAGAAGGTACTGGCTTCCCCGAGAACTCTAAACTGCCCAGTCCCGGGCTACA AGGCCACCGCAGGCTAGCGGCTGCCGACTCCAACATGAGCCCTTCTGCTCCTGGGCTGGGAGGCTGCCAG CTGAGCTACAGCCCCTCCTCCAGCCTCAACAAGAGCAACATGCCTGTGCAGTGGAACGAGGTGAGTTCTG GCACCGTGGATGCCCTGCCCACCCAGGTGAAGCCACCCTCTTTTCCTCAGAGCAACCTGGTTCAGCAGAA GCCAGCCTTTGCCCACTATCCAGGCTATAGCCCACAAGCCCTGCAGAGCGGCTCTGGAGGTCTAGACAGC ACCCAACCACACCTACAGCTTCGAGGGGCCCCCTCTGCATCAAGAGGGAGCTACACGCAACAGCCTCGAC TGCCAGCTACAGGCAGTCAGTGCCTGGGTGTAACTGCAGCCATGAGCCCTCAGGCCAACTACAGCCAAAC CCACCACCAGCTGAGTCCAAACGTCGTCAGTGGATCTCTGAACCAGTTTGCCCCCTCCTGCAGCAATATG GCAGCCAAGCCTAGCCACCTGGGACTCCCTCAGCAAATGGAAGTTGTCCCCAATGCCACCATCATAAGTG GCCATCAACGGGAACTTGGGATTGCCAGTTCATCCCTGGCTGCAGTGTCACAACCTCACCCAGTCCTGGG CTATCCCCAGCAGGACGGCTACCAACAGGTCTCTGGCCTTCCGTCATCCCATCAGCCTAGCTTCATGGAG TCCCAGCAGAACACGGGCTTTGGTCTCATGCAACCTCGGCCGCCCCTGGAGCCCAACACAGCTAGTCGTC ACCGAGGAGTACGTTCTGGCCAACAGCAGCAGCAGCTGTATGCCAGGACCGCTGGCCAAGCCGTGGTCAC ATCAGCCAACCAAGAGACAGCAGAAGCTATGCCCAAGGGAACAGCAGGGACCGTGGTATCCCTAACTCCT CAGCCATCTCAGGACACAGGGCGGTCACAGGACCAGAACACGCTCTACTACTATGGCCAGATCCACATGT ATGAACAGAACGGAGGCTGCCCAGCCATGCAGCCCCAGCCGCCACAACCACAAGGTTGCTCAGACAATAT CCAGCCTGAACCTCTGCCTTCACCAGGAGTCAACCAGGTGTCCAGCACCGTGGACTCCCAGCTCCTGGAG CCCCCCCAGATTGACTTTGATGCCATCATGGACGATGGCGATCACTCGAGTTTGTTCTCTGGTGCACTGA GCCCAAGCCTGCTCCACAATCTCTCCCAGAATTCCTCACGCCTCACCACACCCCGGAATTCCCTGACACT GCCCTCCATCCCCGTGGGCATCAGCAACATGGCTGTTGGGGACATGAGTTCCATGCTCACCAGCTTGGCT GAAGAGAACAAGTTTTTAAACATGATGACCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_001107169
Insert Size	4443 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107169.1, NP_001100639.1
RefSeq Size	6146 bp
RefSeq ORF	4443 bp
Locus ID	304729

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