Ube2f (NM_001008381) Rat Untagged Clone
CAT#: RN208623
Ube2f (untagged ORF) - Rat ubiquitin-conjugating enzyme E2F (putative) (Ube2f), (10 ug)
CNY 3,990.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | RGD1307608 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN208623 representing NM_001008381
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTAACTCTGGCAAGCAAGTTGAAGCGGGATGATGGTCTCAAAGGATCCCGGGCCTCAGCCTCCACAT CCGACTCCACTCGGAGGGTTTCTGTGAGAGACAAGTTGCTTGTTAAAGAGGTTGCAGAACTTGAAGCTAA TTTACCTTATGAGGGTTACTACCAGGGTGGAAAATTTCAGTTTGAAACTGAAGTTCCTGATGCCTACAAC ATGGTGCCTCCCAAGGTGAAATGCTTGACTAAAATCTGGCACCCCAACATCACAGAAACAGGGGAAATAT GCCTAAGTTTACTAAGAGAACATTCAATTGATGGCACTGGCTGGGCTCCCACTAGAACGTTAAAGGATGT TGTTTGGGGATTAAACTCTTTATTTACTGATCTTTTGAATTTTGATGATCCACTGAACATTGAAGCTGCA GAACATCATTTGCGGGACAAGGAAGATTTTCGGGACAAAGTGGATGAATACATCAAACGCTATGCCAGAT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001008381 |
Insert Size | 492 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001008381.1, NP_001008382.1 |
RefSeq Size | 1145 bp |
RefSeq ORF | 492 bp |
Locus ID | 363284 |
UniProt ID | Q5U203 |
Gene Summary | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.[UniProtKB/Swiss-Prot Function] |
Documents
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR208623 | Ube2f (Myc-DDK-tagged ORF) - Rat ubiquitin-conjugating enzyme E2F (putative) (Ube2f), (10 ug) |
CNY 3,990.00 |
|
RR208623L3 | Lenti ORF clone of Ube2f (Myc-DDK-tagged ORF) - Rat ubiquitin-conjugating enzyme E2F (putative) (Ube2f), (10 ug) |
CNY 6,080.00 |
|
RR208623L4 | Lenti ORF clone of Ube2f (mGFP-tagged ORF) - Rat ubiquitin-conjugating enzyme E2F (putative) (Ube2f), (10 ug) |
CNY 6,650.00 |