Ube2f (NM_001008381) Rat Untagged Clone

CAT#: RN208623

Ube2f (untagged ORF) - Rat ubiquitin-conjugating enzyme E2F (putative) (Ube2f), (10 ug)



  "NM_001008381" in other vectors (3)

CNY 3,990.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
Goat Anti-UBE2F / NCE2 (N Terminus) Antibody
    • 100 ug

CNY 5,371.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms RGD1307608
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN208623 representing NM_001008381
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTAACTCTGGCAAGCAAGTTGAAGCGGGATGATGGTCTCAAAGGATCCCGGGCCTCAGCCTCCACAT
CCGACTCCACTCGGAGGGTTTCTGTGAGAGACAAGTTGCTTGTTAAAGAGGTTGCAGAACTTGAAGCTAA
TTTACCTTATGAGGGTTACTACCAGGGTGGAAAATTTCAGTTTGAAACTGAAGTTCCTGATGCCTACAAC
ATGGTGCCTCCCAAGGTGAAATGCTTGACTAAAATCTGGCACCCCAACATCACAGAAACAGGGGAAATAT
GCCTAAGTTTACTAAGAGAACATTCAATTGATGGCACTGGCTGGGCTCCCACTAGAACGTTAAAGGATGT
TGTTTGGGGATTAAACTCTTTATTTACTGATCTTTTGAATTTTGATGATCCACTGAACATTGAAGCTGCA
GAACATCATTTGCGGGACAAGGAAGATTTTCGGGACAAAGTGGATGAATACATCAAACGCTATGCCAGAT
GA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001008381
Insert Size 492 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001008381.1, NP_001008382.1
RefSeq Size 1145 bp
RefSeq ORF 492 bp
Locus ID 363284
UniProt ID Q5U203
Gene Summary Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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