Mcf2l (NM_053951) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	mcf 2l; Ost
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN208691 representing NM_053951 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGTTTTGGCTGAGAAATGAAGAGATGGCCCTAGAAGAAATGGTGCAGAGATTAAACGCGGTTTCCA AGAACACTGATGAAATCATGCACCAGGACATCGTCCCACTCTGTGCCGCTGACATCCAGGAGCAGCTGAA GAAGCGCTTTGCTTACCTGTCTGGTGGGCGGGGACAGGACGGAAGCCCAGTCATCACCTTCCCAGACTAC CCAGCCTTCAGCGAGATCCCGGACAAGGAGTTCCAGAATGTCATGACCTATCTCACCAGCATCCCCAGTT TGCAAGATGCTGGCATCGGGTTCATCTTGGTCATAGACCGAAGACAGGACAAATGGACTTCTGTGAAGGC GTCAGTCCTGCGAATAGCCGCATCATTCCCAGCCAACCTGCAGCTCGTCCTCGTTCTCCGACCCACGGGC TTCTTCCAGAGGACTCTCTCGGACATAGCCTTCAAGTTCAACAGAGATGAGTTTAAGATGAAAGTACCGG TCATGATGCTAAGCTCAGTGCCAGAACTACATGGCTACATTGACAAGTCACAGCTGACCGAGGACCTTGG GGGGACCCTGGACTACTGCCACTCCAGGTGGCTGTGCCACCGCACAGCGATTGAAAGCTTCGCCCTGATG GTGAAGCAGACAGCCCAGATGCTGCAGGCCTTTGGGACCGAGCTGGCCGAGACTGAGCTGCCCAACGACG TCCAGTCAACCAGCCTGGTGCTCAGCGCACACACAGAGAAGAAGGCTAAAGTGAAGGAGGATCTGCAGCT GGCGCTGACGGAGGGGAACAGCATCCTTGAGAGCCTCAGGGAGCCGCTGGCTGAGAGCATAGTCCACAGT GTGAACCAGGACCAGCTGGACAATCAGGCCACTGTGAAGAGGCTCCTGACCCAGCTGAATGAGACCGAGG CTGCCTTTGACGAGTTCTGGGCAAAGCATCAGCAGAAGCTGGAACAGTGCCTACAGCTGCGACATTTTGA ACAAGGTTTCCGGGAGGTCAAAACCGCCTTGGATTCCATGTCCCAGAAGATAGCCGCGTTCACAGATGTT GGCAACAGCCTGGCCCATGTGCAGCACCTCCTGAAGGACTTGACCACCTTTGAGGAGAAGTCCAGCGTGG CCGTGGACAAGGCCAGAGCCCTGTCCCTGGAGGGGCAGCAGCTGATTGAGAACCGGCACTATGCTGTAGA CTCTATCCATCCGAAGTGTGAGGAACTCCAGCACCTCTGTGACCACTTCGCCTCTGAGGTCACCAGGAGG CGGGACCTTCTCAGCAAGTCCTTGGAGCTGCATAGCCTCCTGGAGACGTCCATGAAGTGGAGCGACGAGG GCATCTTCCTGCTGGCCTCTCAGCCTGTGGACAAATGCCAATCTCAGGATGGTGCGGAGGCAGCCCTGCA GGAGATCGAGAAGTTTCTGGAGACCGGTGCAGAAAACAAGATTCAGGAGCTCAATAAGATTTACAAGGAA TATGAATGCATCCTCAACCAGGACCTTCTGGAACACGTGCAAAAAGTCTTTCAGAAGCAAGAGAGCACCG AGGAGATGTTCCACCGCAGGCAGGCTAGCCTGAAGAAACTGGCAGCCAAGCAGACACGGCCTGTGCAGCC CGTGGCCCCCCGACCAGAGGCGCTAACCAAGTCCCCCTCCCCCTCGCCAGGCTCCTGGCGAAGCTCTGAG AACTCCAGCTCTGAGGGCAACGCCCTCCGCAGAGGGCCCTACAGGAGAGCCAAGAGCGAAATGAGTGAGC CCCGGCAGGGCCGCACCAGCTCCACAGGAGAAGAGGAGGAGAGCCTGGCCATCCTGCGCAGACACGTGAT GAATGAACTTCTGGACACGGAGCGGGCCTACGTGGAGGAGCTGCTCTGTGTCTTAGAGGGCTATGCTGCA GAGATGGACAACCCCTTGATGGCACACCTCATCTCAACAGGCCTGCAAAACAAGAAGAACATTCTGTTTG GAAACATGGAGGAAATCTATCACTTCCACAACAGAATATTCCTGCGGGAGCTGGAGAGCTGCATCGACTG CCCAGAGCTGGTGGGGAGATGCTTTCTGGAGAGGATGGAGGAGTTCCAGATCTATGAGAAGTACTGTCAG AACAAGCCACGCTCCGAGAGCCTGTGGAGACAGTGCTCTGACTGTCCCTTCTTCCAGGAGTGCCAGAAGA AGCTGGACCACAAGCTGAGCCTCGACTCCTACCTGCTGAAGCCTGTGCAGAGGATAACCAAGTACCAGCT GCTGCTCAAGGAAATGCTGAAGTACAGCAAGCACTGTGAAGGGGCCGAGGACCTGCAAGAGGCACTGAGC TCCATCCTGGGCATCCTCAAGGCAGTGAACGACTCCATGCACCTCATAGCCATCACTGGCTATGATGGAA ACCTTGGGGACCTGGGGAAGCTGCTGATGCAAGGCTCCTTCAGCGTGTGGACGGACCACAAGAAGGGCCA CACCAAGGTGAAGGAGCTAGCCAGGTTCAAGCCCATGCAGCGGCACCTCTTCCTTCACGAGAAGGCCGTC CTCTTCTGCAAGAAGAGGGAGGAGAATGGGGAGGGGTACGAGAAGGCTCCTTCCTACAGCTACAAGCAGT CCTTGAATATGACTGCTGTTGGCATCACAGAGAATGTGAAGGGTGACACCAAGAAGTTCGAGATCTGGTA CAACGCAAGGGAGGAGGTTTACATCATTCAGGCACCAACTCCCGAGATTAAAGCAGCGTGGGTGAATGAG ATTCGGAAGGTGCTGACCAGCCAACTGCAGGCCTGCCGGGAAGCCAGCCAGCACCGAGCCCTGGAGCAGT CCCACAGCCTTCCCTTGCCCACGCCAGCCAGCACCAGTCCCACAAAAGGGAGCACAAGAAATGTCAAGAA GCTCGAAGATCGGAAGACGGACCCGCTCTGCCTGGAAGGCTGCGTGAGCTCATCATTACCAAAGCCCCCC GAGAAGGGCAAAGACGATGCGGTCCCTAGTTCTACCTCTGAAAGCTCTGCGCTTTCCAGAAAGCGCTTCA CGCTGCAGGGCTTGGCTAACCTCAAAGCCTCTCCGACCAGTCCTGACAAAAAAGCTAAGCGGCATGAAGT AAAGAGCGACCCGACTCCCTTTGGTTTGCGAGGCTGGAGCAAAACATCCCACTCACTGGAGGCCCCTGAG GAGGACGGAGGCTGGTCCAGTGCTGAGGAGCTCATCAATTCATCCGATGCTGAGGAAGACGGAGGAGTGG GCCCTAGGAAGCTGGTTCCAGGTAAATACACAGTGCTGATGGATGGTGAGAAAGGGGGCTCTGACACCCT TGCCATGAGGAGTGGAGATATGGTGGAGGTTGTGGAAGAAGGAACTGAGGGCCTCTGGTATGTTCGGGAC CTGACCAGCAGCAAGGAGGGATGGGTGCCAGCCAGCAGCCTAGCCACCCTCCTGGGCAAATCCAGCTCAG CCCAGTGTCTGAGCAGCTCAGGTAAGACCCACTGTGCGCGCCAGCTGTGCCCTGAGCCTGCCAAGATCCT CAGCCCAGAGCCTGTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053951
Insert Size	3519 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053951.2, NP_446403.2
RefSeq Size	4531 bp
RefSeq ORF	3519 bp
Locus ID	117020
Gene Summary	oncogene that can activate small GTP-binding proteins [RGD, Feb 2006]

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