Slc1a1 (NM_013032) Rat Untagged Clone

CAT#: RN209021

Slc1a1 (untagged ORF) - Rat solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 (Slc1a1), (10 ug)



  "NM_013032" in other vectors (3)

CNY 5,848.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit Polyclonal Anti-Excitatory Amino Acid Transporter 3
    • 50 ul

CNY 11,000.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Eaac1; Eaat3; REAAC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN209021 representing NM_013032
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAAGCCCACGAGCTCGGGATGCGACTGGCGCCGCTTCCTGCGGAATCACTGGCTGCTGCTCTCCA
CCGTGGCCGCCGTGGTACTGGGCATCGTGGTAGGAGTCTTGGTTCGAGGACACAGTGAGCTCTCGAATCT
GGATAAATTCTACTTTGCGTTTCCTGGAGAAATTCTGATGCGGATGCTGAAGCTCGTCATTCTGCCACTG
ATTATATCCAGCATGATCACAGGTGTCGCTGCACTGGATTCCAACGTATCTGGAAAAATTGGTCTTCGTG
CTGTAGTATATTATTTCTCCACCACCGTCATTGCTGTAATCCTAGGTATTGTGTTAGTTGTGAGCATCAA
GCCTGGTGTCACTCAGAAAGTGAATGAAATCAACAGGACAGGCAAAACCCCTGAAGTCAGCACTGTGGAC
GCCATGTTGGATCTGATCAGGAACATGTTCCCTGAGAACCTGGTCCAAGCCTGTTTTCAGCAGTACAAAA
CCAAGCGTGAAGAAGTGAAGCCTGCCAGTGATCCTGGGGGGAACCAGACAGAGGTGTCTGTCACCACGGC
CATGACAACAATGTCTGAGAACAAGACAAAGGAATACAAAATCGTGGGCCTGTACTCAGACGGCATCAAC
GTCCTGGGCCTGATTATCTTCTGCCTCGTCTTTGGACTTGTCATTGGAAAAATGGGAGAAAAAGGGCAGA
TTCTGGTGGATTTCTTCAATGCGTTGAGTGACGCCACCATGAAAATCGTTCAGATCATCATGTGTTACAT
GCCGATCGGTATTTTGTTCCTAATCGCTGGGAAGATCATAGAAGTTGAAGACTGGGAAATATTCCGCAAG
TTGGGCCTTTATATGGCCACTGTCCTGAGTGGGCTTGCAATCCACTCCCTCGTAGTGCTGCCACTGATCT
ATTTCATAGTCGTGCGGAAGAACCCTTTCCGCTTTGCCTTGGGCATGGCGCAGGCTCTCCTGACAGCTCT
CATGATCTCATCTAGCTCGGCAACCCTTCCAGTTACATTCCGCTGTGCGGAAGAAAAGAACCACGTGGAC
AAGAGGATCACAAGATTTGTGCTGCCCGTCGGCGCCACCATCAACATGGACGGCACTGCGCTCTATGAAG
CCGTGGCAGCTGTGTTCATTGCGCAGCTGAACGGCATGGACCTGAGCATTGGGCAGATCATCACGATCAG
CATCACAGCCACCGCTGCTAGCATTGGAGCTGCCGGTGTGCCCCAGGCTGGCCTGGTGACCATGGTGATC
GTGTTGAGTGCCGTGGGGCTGCCTGCTGAGGACGTCACCCTCATCATTGCCGTCGACTGGCTCCTGGACC
GGTTCAGGACCATGGTGAACGTTCTGGGTGATGCGTTTGGGACGGGCATCGTGGAGAAGCTGTCCAAGAA
GGAGCTGGAGCAGGTGGATGTCTCCTCTGAAGTCAACATTGTGAACCCCTTTGCCTTGGAGCCCACAATC
CTGGATAATGAAGACTCAGATACGAAGAAGTCTTACGTCAATGGGGGCTTCTCGGTAGACAAATCTGACA
CCATCTCGTTCACTCAGACCTCGCAGTTCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_013032
Insert Size 1572 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_013032.3, NP_037164.3
RefSeq Size 1821 bp
RefSeq ORF 1572 bp
Locus ID 25550
UniProt ID P51907
Gene Summary mediates sodium ion dependent high affinity glutamate transport and proton cotransport; plays a role in maintenance of neuronal function [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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