Myh2 (NM_001135157) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Myh1; Myh2a; MyHC-2A; MyHC-IIA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209111 representing NM_001135157 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCCGACTCTGAGATGGCCGTTTTCGGGGAGGCTGCTCCTTACCTCCGGAAGTCTGAAAAGGAGC GAATCGAGGCCCAGAATAGGCCTTTTGATGCCAAAACATCTGTCTTTGTGGCGGAGCCCAAGGAGTCCTT TGTCAAAGGAACCATTCAGAGCAAAGACGCAGGGAAAGTGACTGTGAAAACTGAAGCAGGAGCGACCCTG ACGGTGAAAGAAGACCAGATCTTCCCCATGAACCCTCCCAAGTACGACAAGATCGAGGACATGGCCATGA TGACCCACCTCCACGAGCCCGCTGTGCTGTACAACCTCAAAGAGCGTTACGCAGCCTGGATGATCTACAC CTACTCGGGCCTCTTCTGTGTCACTGTCAACCCCTACAAGTGGCTGCCAGTGTACAACCCTGAGGTGGTG GCTGCCTACCGTGGCAAAAAGCGACAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGACAATGCCTACC AGTTCATGCTAACTGACCGGGAGAATCAGTCAATCCTGATCACCGGAGAATCCGGGGCCGGGAAGACTGT GAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGAGAAAAAGAAGGAGGAGGTG ACTTCTGGCAAAATGCAGGGGACCCTGGAAGATCAAATCATCAGTGCCAACCCTCTGCTGGAGGCCTTTG GGAATGCCAAGACCGTGAGGAATGACAACTCCTCTCGCTTTGGTAAATTCATCAGGATTCACTTTGGCAC CACGGGGAAACTGGCATCCGCTGACATCGAGACATATCTGCTAGAGAAGTCCCGGGTCACTTTCCAGCTT AAGGCGGAAAGGAGCTACCATATATTTTATCAAATCACATCCAACAAGAAGCCAGAGCTGATCGAAATGC TGCTGATCACCACAAACCCATACGATTACCCATTCGTCAGTCAAGGGGAGATCAGTGTGGCCAGCATTGA TGACCAGGAAGAACTGATGGCCACGGATAGTGCTATTGACATTCTGGGCTTTACAAATGATGAAAAGGTC TCCATCTATAAGCTCACGGGAGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAGAAGCAGCGGGAAG AACAGGCTGAGCCGGATGGCACGGAAGTGGCTGACAAGGCTGCCTATCTTCAGGGTCTGAACTCTGCTGA CCTGCTCAAAGCCCTTTGTTACCCCAGGGTCAAGGTCGGCAATGAGTACGTCACCAAAGGCCAGACTGTG GAGCAGGTGACCAACGCTGTGGGTGCCCTGGCCAAGGCCATGTACGAGAAGATGTTCCTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCAGGCAGTACTTCATCGGGGTCTTGGACATCGCTGG CTTTGAGATCTTTGATTTCAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACCTTCA TCGACTTCGGGATGGACCTGGCGGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCT GGAAGAGGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGAACAGCATCTT GGAAAGTCCGCCAACTTCCAGAAGCCTAAGGTGGTCAAAGGCAAAGCCGAGGCCCACTTCTCCCTCATCC ACTATGCGGGCACCGTGGATTACAACATCACTGGCTGGCTGGACAAGAACAAGGACCCCCTGAATGAGAC CGTGGTGGGGCTGTACCAGAAGTCTTCAATGAAAACTCTTGCTTACCTCTTCTCTGGAGCTCAAACTGCT GAAGCAGAGGCAAGTAGTGGTGGAGCTGCCAAGAAAGGTGCTAAGAAGAAAGGGTCCTCCTTCCAGACAG TGTCTGCCCTGTTCAGAGAGAACTTGAACAAGCTGATGACCAACCTCAGGAGCACCCACCCTCATTTTGT GAGGTGTATCATCCCCAATGAAACCAAGACACCTGGTGCCATGGAGCACGAACTGGTCCTGCACCAGCTG AGGTGTAACGGGGTGCTAGAAGGCATCCGCATCTGCAGGAAGGGGTTCCCCAGCAGGATCCTCTACGCAG ACTTCAAGCAGAGATACAAGGTGTTAAATGCAAGTGCCATCCCTGAGGGTCAGTACATAGATAGCAAGAA GGCTTCTGAGAAGCTCCTTGGCTCCATCGACATTGACCACACTCAGTATAAGTTTGGTCACACCAAGGTC TTTTTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATGAGAGATGACAAGTTGGCCCAGCTGATTACCC GGACGCAGGCCATGTGCAGAGGGTTCTTGGCAAGAGTGGAGTACCAGAAGATGGTGGAGCGAAGAGAGTC CATCTTCTGCATCCAGTACAACATCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTC TTCAAGATCAAACCACTGCTGAAGAGCGCTGAGACCGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCC AGAAAACCAAGGATGAACTTGCCAAATCGGAGGCAAAGAGGAAGGAACTTGAAGAAAAGATGGTGTCCCT GTTGAAAGAAAAAAATGACTTGCAACTCCAAGTTCAGGCTGAAGCTGAAGGCTTGGCTGATGCTGAGGAA AGATGCGACCAGCTGATCAAAACCAAAATCCAGCTGGAGGCCAAAATCAAAGAGGTGACTGAGAGAGCTG AGGATGAGGAAGAGATCAACGCCGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCT GAAGAAAGACATCGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAAC AAGGTGAAAAACCTCACAGAGGAGATGGCGGGCCTGGACGAAACCATCGCCAAGCTGACCAAGGAGAAGA AGGCCCTGCAGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCT GACCAAAGCCAAAATCAAGCTGGAACAGCAAGTGGATGATCTTGAAGGGTCCTTGGAGCAAGAAAAGAAG CTTCGCATGGACCTAGAAAGAGCCAAGAGAAAACTTGAGGGTGACTTGAAGTTAGCCCAGGAGTCCATCA TGGACATTGAAAATGAGAAACAGCAGCTTGATGAAAGGCTCAAAAAGAAAGAGTTTGAAATGAGCAACCT GCAGAGCAAGATCGAGGATGAGCAGGCCATCGGCATTCAACTGCAGAAGAAGATCAAGGAGTTGCAGGCT CGCATTGAGGAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCCAAAGCAGAGAAGCAGCGCT CTGACCTCTCCCGGGAACTGGAGGAGATCAGCGAGCGGCTGGAAGAAGCCGGCGGGGCCACTTCAGCCCA GATCGAGATGAACAAGAAGAGAGAGGCCGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACCCTG CAGCACGAAGCCACAGCAGCCACCCTGAGGAAGAAGCACGCGGACAGCGTGGCTGAGCTCGGGGAGCAGA TTGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGATGAAGATGGAGATCGACGA CCTCGCTAGTAATGTAGAGACAGTGTCTAAGGCCAAGGGAAACCTAGAGAAGATGTGCCGCACCCTGGAG GACCAGGTGAGTGAGCTGAAGTCGAAGGAGGAGGAACAACAGCGCCTGATCAACGACCTGACCACCCAGA GAGGACGCCTGCAGACCGAATCCGGTGAATTTTCCCGGCAGCTTGATGAGAAGGAAGCGCTGGTTTCTCA GTTATCAAGGGGCAAGCAAGCATTTACCCAACAGATTGAGGAGCTGAAGAGGCAGCTGGAAGAGGAAGTA AAGGCCAAGAACGCGCTAGCCCACGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTTCGGGAACAGT ACGAGGAGGAGCAGGAATCTAAGGCCGAGCTGCAGAGGGCGCTGTCCAAGGCCAACAGTGAGGTGGCCCA GTGGAGGACCAAATATGAGACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAGGCCAAGAAAAAGCTG GCTCAGCGTCTGCAGGCGGCTGAGGAGCACGTAGAAGCCGTGAACGCCAAGTGTGCTTCCCTGGAGAAGA CTAAGCAGCGGTTGCAGAACGAGGTGGAGGACCTCATGCTGGACGTAGAGAGGACCAACGCTGCCTGTGC TGCCCTGGACAAGAAGCAGAGAAACTTCGACAAGATCCTGGCAGAGTGGAAGCAGAAGTATGAGGAAACA CATGCTGAGCTGGAGGCATCCCAGAAGGAAGCCCGCTCCCTGGGTACTGAGCTCTTCAAGATGAAGAACG CCTACGAGGAGTCCCTGGATCAGCTGGAAACCCTGAAGCGAGAGAATAAGAACTTACAGCAGGAGATTTC TGACCTCACGGAACAGATTGCAGAAGGAGGAAAGCGCATCCACGAACTGGAGAAAATAAAGAAACAAGTC GAACAAGAGAAGTGTGAACTTCAGGCTGCTCTAGAAGAAGCAGAGGCATCTCTGGAGCACGAGGAGGGAA AGATCCTGCGCATCCAGCTGGAGCTGAACCAAGTCAAGTCTGAGATCGACAGGAAGATTGCTGAGAAGGA TGAGGAGATCGACCAGCTGAAGAGAAACCACATTCGAGTCGTGGAGTCCATGCAAAGCACGCTGGACGCC GAGATCAGGAGCAGGAACGACGCCATCAGGATCAAGAAGAAGATGGAGGGAGATCTCAATGAGATGGAAA TCCAGCTGAACCACGCCAACCGCATGGCTGCTGAGGCCCTGAGAAACTACAGGAACACCCAAGGCATCCT CAAGGACACCCAGCTGCACCTGGATGACGCTCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCCATG GTGGAGCGCAGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACTCTGGAGCAGACTGAGA GGAGCAGGAAGATTGCAGAGCAGGAACTGCTGGACGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAGAA CACCAGCCTCATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAGATCCAGGGAGAGATGGAGGAC ATCGTCCAGGAAGCCCGCAATGCAGAAGAGAAGGCCAAGAAAGCCATCACTGACGCCGCCATGATGGCGG AGGAGCTGAAGAAGGAGCAGGACACCAGCGCCCATCTGGAGCGGATGAAGAAGAACATGGAGCAGACGGT GAAGGACCTGCAACTGCGTCTGGACGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAG AAACTGGAGGCCAGAGTGCGTGAACTGGAGGGTGAGGTAGAGAGTGAGCAGAAGCGGAATGTTGAGGCTG TCAAAGGGCTTCGAAAACACGAGAGGCGAGTGAAGGAGCTTACTTACCAGACAGAAGAAGACCGAAAAAA TATCCTCAGGCTTCAAGATTTGGTGGATAAACTCCAAGCAAAAGTAAAATCTTACAAGAGACAAGCTGAG GAGGCTGAGGAACAATCCAACACAAATCTATCCAAGTTCCGCAAGCTGCAGCATGAGCTGGAGGAAGCCG AGGAGCGGGCTGACATCGCCGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGCGAGGTTCACACTAA AGTCATAAGTGAAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001135157
Insert Size	5829 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001135157.1, NP_001128629.1
RefSeq Size	6041 bp
RefSeq ORF	5829 bp
Locus ID	691644
Gene Summary	mouse homolog is a heavy chain skeletal muscle myosin [RGD, Feb 2006]

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