Col17a1 (NM_001106366) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209139 representing NM_001106366 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGTGACCAAGAAAAGCATGCGAGATGGCACTGAAGTCACTGAGAGAATTGTCACGGAAACAGTAA CCACAAGACTCACATCCTTACCACCAAAAGGGAGCACCAGCAATGGATACACTAAGACAGGCTCTCTGGG TGGAGGAAGCCGGCTAGAGAAACAAAGTTTGACCCATGGAAGCAGCGGCTACATCAACTCAAGCGGAAGC CTCCGAGGCAACGCTTCCACCTCCAGTTACAGGAGAGCTCACTCACCTGCCTCGACCCTGCCCAACTCTC CCGGCTCCACCTTTGAAAGGAAAACTCACATGAGCCGCCATGGAACTTACGAAGGGAGCTCCAGTGGCAA CTCCTCACCTGAGTATCCACGGAAGGAACTCGCCTCTTCTTCAACCAGAGGACGAAGCCAGACACGAGAG AGCGAAATTCGAGTTCGACTACAGAGTGCGTCGCCATCCACCAGATGGACAGAACTGGATGAAGTGAAGC GCTTGCTGAAGGGGAGCCGATCTGCCAGTGCCAGCCCCACCAGGAACACCTCCAACACACTCCCTATCCC CAAGAAAGGCACCGTAGAAACCAAAACGGTGACAGCGAGCTCCCACTCAGTGTCAGGAACCTATGATTCA ACAATACTGGACAGCAACTTTCCCCCCCACATGTGGTCTTCCACCTTGCCGGCAGGGTCCTCCATGGGGA CCTATCACAACAACATAGCGACCCAGAGCACATCCCTCGTCAACACCAATGCCTACTCAGCAGGATCAGT CTTTGGAGTGCCAAATAACATGGCGTCCTGCTCTCCCACCCTGCACCCAGGACTCAGCAGCTGCTCCTCA GTGTTTGGCATGCAGAACAATCTGGCCCCCTGCTCTTCTGTCCTCTCCCATGGCACAACCACGGCTTCCA CAGCATATGGTGTGAAGAAAAACGTGCCCCAGCCACCCACTGTCACCAGCACCGGCGTGTCCACCTCTGC CGCCTGCACCACCAGTGTCCAGAGCGACGACCTCTTGCATAAAGACTGCAAGTTCCTGATTCTGGAGAAA GACAACGTGCCCGCCAAGAAGGAGATGGAGCTCTTGATCATGTCCAAGGACAGTGGGAAGGTCTTCACTG CCTCCCCCGCCAGCATCTCTTCAACTTCTTTTTCAGAGGACACCCTGAAAAAGGAGAAGCAGGCTGCGTA TGCCGCTGACACATGTCTAAAGGCAGATGTGAACGGAGACCTAAACACTGTGTCTACGAAGAGCAAAGCC ATCTCGGCAGAAAACCACAGCTACGACCGAGGTGTCGGTGGTGGCAGAGGCAAAAGCGGAGTCGCCGGCG GTGGTGGTGGTGGTGGCGGCGGCGGCAGCGGCGGCGGAACATGGGGGGCTGCACCTGCCTGGTGCCCCTG CGGCTCCTGCTGCAGCTGGTGGAAGTGGCTGCTGGGCCTGCTGCTCACTTGGCTGCTGCTGCTGGGTCTG CTCTTCGGCCTCATTGCCCTGGCGGAGGAGGTAAGGAAGCTGAAGGCCCGCGTGGAGGAGCTGGAAAAGA CCAAGGTGCTATATCATGACATCGTCAAGACGGACCGAAGCAGCAGGGACCGCCTCCAGGGTGAGGCACC CAGCCTGGGACCTGGATTAAGCAAGGCTGAAATGGACGACTACGGCCAGGAGGCCATCTGGCTGTTTGTA AGGAACAAGCTGATGACAGAGCAGGAGAACGGAAATCTCAGAGGAAGTCCTGGTCCAAAAGGTGACACGG GGAGTCAAGGACCTAAAGACCGTGGCCTTCCTGGGATCCCAGGTACCCCTGGGCCCCTGGGCCACCCCGG CCCGGAAGGACCAAAGGGACAAAAAGGCAGCATTGGAGATCCAGGCATGGAAGGACCCATAGGCCAGAGA GGACTAGCAGGCCCCATGGGACCTCCTGGTGAACCTGGGCCTCCGGGGTCTGGAGAGAAAGGAGACAGAG GGATTGCTGGAGAACAAGGTCCTCGGGGTCTTCCCGGTGTCCCGGGTTCTCCGGGTCTCAGAGGTACCAG TGGCTCTCCTGGTCCCCAGGGCCCCCCAGGCTCTGTGGGTCCCCAAGGGCTCCGAGGTGAAGTGGGACTT CCGGGTGTCAAAGGTGACAAAGGACTCGTGGGACCACCAGGACCCAAAGGTGACCAGGGTGAGAAAGGAC CCAGAGGCCTCACAGGGGAGCCTGGCATCCGAGGTCTGCCTGGAGCTGTGGGTGAACCTGGAGCCAAAGG AGCAATGGGTCCCGCTGGCCCCGATGGACAGCAAGGTTCCAGAGGTGAACAAGGCCTGACTGGGATGCCT GGAGCCCGGGGCCCACCAGGACCCACCGGAGACCCAGGGAAGCCAGGTCTCACAGGACCCCAAGGACCTC AGGGACTTCCTGGTACCCCTGGCCGACCAGGGACTAAAGGTGAACCTGGCGCTCCAGGCAGAGTCATGAC TTCAGAGGGATCCTCAGCAATCACTGTGCCGGGACCTCCCGGACCTCCCGGTGCCATGGGACCCCCAGGA CCTCCAGGGACTCCAGGTCCGGCTGGCCCTGCTGGTCTCCCAGGACAACAAGGCCCACGAGGGGAGCCGG GACTTGCTGGGGACTCCTTCCTAAACAGTGGCAGTTCCATCTCCGAAGTCCTCTCTGCCCAAGGTGTTGA CTTTCGAGGTCCCCCTGGCCCACCTGGCCCACGAGGGCCACCAGGGCCTTCCATTCCAGGCCCACCAGGA CCCAGAGGCCCACCAGGGGAAGGCGTACCAGGCCCACCCGGCCCACCAGGATCCTTCCTGACTGACTCGG AAACCTTCTTCTCTGGCCCTCCAGGTCCACCTGGCCCCCCAGGTCCCAAGGGAGACCAAGGTGACCCCGG TGTCCCAGGCACTCCTGGCATCCCTGGTGGTCTTTCTCATGGGACGTCGTCAGGCACTATGTACGCACAG GGCCCACCTGGTCCCCCAGGACCTCCTGGGCCTCCAGGATCTCTCAGCAGCTCCGGCCAGGAACTCCAGC GCTATATTGCAGAGTACATGCAAAGTGACAACATCAGGACTTATCTCTCAGGAGTTCAGGGTCCCCCCGG CCCACCAGGTCCCCCAGGGCCTGTCATCACCATCACAGGGGAGACTTTCGACTACTCCCAGCTGGCAAGC CAGGTTGTGAGTTACTTGCGATCATCAGGCTATGGTGTTGGCTTGTCCTCTGCCTCCTCCTCAGAAGATA TCTTGGCCATGCTGAGACGCAATGATGTGTGGCAGTACCTACGTCAGCACCTGGTGGGCCCTCCGGGTCC CCCGGGACCACCAGGAGTCAGTGGAGATGGGTCCCTCCTGTCTTTGGACTATGGAGAGCTAAGCAGACAC ATTCTCAACTATATGTCAAGTTCTGGGATCAGCTTTGGGCATCCTGGACCACCGGGCCCCCCTGGCCTGC CAGGAACATCCTATGAGGAGCTACTGACCATGCTCCGAGCTGCTGGTCTGTCCTCCATCGCAGGCCCCCC GGGCCCCCCAGGTCCCCCAGGTCCTCGAGGGCCCCCGGGTGTGTCAGCAGCTCTGGCCACCTACGCAGCT GAGAACAGTGACAACTTCCGCAGTGAGCTGATTAGCTACCTTACAAGTCCTGATGTCCGCAGCTTCATCG TTGGCCCTCCAGGTCCTCCAGGGCCACAGGGACCACCTGGAGACTACAACTGGGGTAGCGGCTCCTCAGC CAGAAGGGGCACTTCCTACAGCTCTTCCATGGGCACAGGGAGAACCAATGGTGGCTCCCTGGGCGAAGGT GGAGCCTTTGGTGCAGGAGATGGGGGCCCCTACGGCACTGACGTCGGCCCAGGCGGAGGCTACGGGGCAG CTGCAGGTGGTGGTGTATATGGCACCAATGGCGAATCATTCCGGGGCAGCTTCACTGGAGACCTAGATTA TAACACGCTGGCATTGCGGGTGTCGGAGAGCATGCAGCGTCAGGGTCTGCTACAAGGGATGGCCTACACT GTCCAGGGCCCACCAGGGCCACAAGGCCCTCCTGGCATCAGCAAGGTCTTCTCTGCCTACAGCAACGTGA CACAAGACCTCATGGACTTCTTCCGAACATATGGCGCTATCCCAGGACCCCCTGGGCAGAAAGGAGACAT GGGAACCCCAGGTCCCAAAGGTGACAGGGGCCCTGCTGGACCACGAGGTCCTCCGGGGCCCCCGGGCCCC CGAGGGAACAAAGGAGAGAAAGGGGATAAAGGTGACCAAGTCTATACTGGGAGGAGGAAGAGAAGTATTG CCATCAAGCCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001106366
Insert Size	4284 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001106366.1, NP_001099836.1
RefSeq Size	5433 bp
RefSeq ORF	4284 bp
Locus ID	294027

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