Dscam (NM_133587) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209167 representing NM_133587 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGATACTGGCTCTCTCCTTGTTCCAGAGCTTCGCGAATGTTTTCAGTGAAGAGCCCCACTCCAGCC TCTACTTTGTCAATGCATCGCTGCAAGAGGTAGTGTTTGCAAGCACGTCGGGGACCCTGGTGCCCTGCCC GGCTGCAGGCATCCCTCCTGTGACTCTCAGATGGTACCTAGCAACGGGCGAGGAGATCTACGATGTCCCT GGGATCCGCCACGTCCATCCCAATGGCACTCTCCAAATTTTCCCCTTTCCTCCTTCAAGCTTCAGCACCT TAATCCATGATAATACTTACTATTGCACAGCTGAAAACCCTTCAGGGAAAATTAGAAGTCAGGATGTCCA CATCAAGGCTGTTTTACGGGAGCCCTATACAGTCCGTGTGGAGGACCAGAAAACCATGAGAGGCAATGTC GCGGTGTTCAAGTGCATTATCCCCTCCTCGGTGGAGGCGTACGTCACTGTCGTCTCATGGGAGAAAGACA CGGTTTCACTTGTCTCAGGATCTAGATTTCTCATCACATCCACGGGAGCCTTGTATATTAAAGATGTACA GAACGAAGATGGGCTGTACAACTACCGCTGCATCACGCGGCACAGATACACAGGGGAGACGAGACAAAGC AACAGCGCGAGACTGTTCGTGTCAGACCCAGCGAACTCAGCCCCATCCATCCTGGACGGGTTTGACCACC GCAAAGCCATGGCAGGGCAGCGTGTGGAGCTGCCGTGCAAAGCTCTTGGGCACCCCGAGCCGGACTACCG CTGGCTGAAAGATAACATGCCCCTGGAACTTTCCGGAAGGTTCCAAAAGACAGTGACCGGGCTGCTCATT GAGAACAGCCGCCCTTCAGACTCAGGCAGCTACGTGTGTGAAGTATCCAACCGATACGGCACTGCCAAGG TGATAGGCCGCCTGTACGTGAAACAGCCACTGAAAGCCACCATCAGTCCCAGAAAGGTTAAAAGCAGTGT GGGCAGCCAGGTCTCCTTGTCCTGCAGTGTGACAGGAAACGAAGACCAGGAGCTCTCCTGGTACAGAAAT GGTGAAATCCTCAACCCTGGGAAAAACGTGAGGATCACAGGACTCAACCACGCGAACCTTATAATGGATC ACATGGTCAAGAGTGATGGGGGCGCCTACCAGTGCTTTGTTCGCAAGGACAAGCTATCTGCTCAAGACTA TGTCCAGGTGGTCCTTGAAGACGGAACTCCCAAAATCATTTCCGCCTTTAGCGAGAAAGTGGTGAGCCCG GCAGAGCCAGTGTCCCTCGTGTGTAATGTGAAGGGTACACCCTTGCCCACGGTCACCTGGACCCTGGACG ATGACCCCATCCTCAAGGGCAGCGGCCACCGCATCAGCCAAATGATCACGTCCGAGGGGAACGTGGTCAG CTACCTGAATATCTCCAGCTCCCAGGTCCGGGATGGGGGTGTCTACCGCTGCACTGCCAACAACTCGGCT GGAGTCGTCCTGTACCAGGCTCGAATAAACGTAAGAGGGCCGGCAAGCATCAGACCGATGAAAAACATCA CTGCGATAGCAGGGCGTGACACGTACATCCACTGCCGTGTGATTGGCTACCCTTATTACTCCATCAAGTG GTACAAGAACGCTAACCTGCTTCCTTTCAACCACCGCCAGGTGGCGTTTGAGAACAATGGGACTCTGAAA CTCTCAGATGTGCAGAAAGAAGTTGACGAGGGAGAGTACACATGTAACGTGCTGGTGCAGCCACAGCTCT CCACCAGCCAGAGTGTCCACGTGACAGTCAAAGTTCCTCCTTTTATCCAACCCTTTGAGTTCCCAAGATT CTCTATCGGTCAGCGGGTCTTCATCCCATGTGTGGTGGTCTCGGGGGACTTACCCATCACCATCACCTGG CAGAAGGATGGCCGGCCGATTCCAGCAAGCCTCGGTGTAACCATCGACAACATCGACTTCACCAGCTCCC TGAGGATCTCCAACCTCTCACTGATGCACAACGGGAATTACACCTGCATCGCGCGGAACGAGGCGGCCGC CGTGGAACACCAGAGCCAGCTGATTGTAAGAGTTCCCCCTAAGTTCGTGGTCCAGCCCCGGGACCAGGAT GGGATCTATGGCAAAGCAGTGATTCTCAATTGCTCTGCAGAGGGTTATCCTGTGCCTACAATTGTGTGGA AATTCTCAAAAGGTGCTGGGGTCCCCCAGTTCCAGCCAATTGCCCTGAATGGCAGAATCCAGGTCCTGAG TAATGGCTCACTCTTGATCAAGCATGTTGTGGAAGAAGACAGTGGCTACTACCTCTGCAAGGTCAGCAAC GATGTGGGCGCAGACGTCAGCAAGTCCATGTACCTCACGGTGAAAATTCCTGCCATGATAACCTCTTATC CCAACACCACCCTGGCCACTCAGGGTCAAAGGAAGGAGATGAGCTGCACAGCACACGGGGAGAAGCCCAT CATTGTCCGCTGGGAGAAAGAGGACAGGATCATTAACCCTGAAATGGCCCGTTACCTGGTGTCCACCAAG GAGGTGGGAGAGGAAGTGATTTCTACTCTGCAGATTTTGCCAACAGTGAGAGAAGATTCCGGCTTCTTCT CCTGCCATGCTATCAATTCATATGGGGAGGACCGCGGAATAATTCAGCTCACGGTGCAAGAACCCCCAGA CCCCCCCGAGATTGAGATCAAAGATGTCAAAGCCCGCACAATCACGCTCAGGTGGACCATGGGGTTTGAT GGCAACAGCCCCATCACAGGCTACGACATTGAGTGCAAAAATAAATCAGACTCCTGGGATTCTGCTCAAA GAACCAAAGATGTTTCCCCTCAGCTGAACTCGGCCACCATCATTGATATCCACCCTTCCTCCACCTACAG CATCCGCATGTACGCCAAGAACCGGATTGGCAAGAGTGAGCCCAGCAACGAGATCACCATCACGGCAGAT GAGGCAGCTCCTGATGGTCCACCTCAGGAAGTGCACCTGGAACCCACCTCATCTCAGAGTATCAGGGTTA CCTGGAAGGCTCCCAAGAAACATTTACAAAATGGGATCATACGTGGCTACCAGATAGGCTACCGGGAGTA CAGCACCGGGGGCAACTTCCAGTTCAACATCATCAGTATCGACACCACCGGGGACAGCGAAGTGTACACC CTGGACAACCTGAATAAGTTCACCCAGTATGGCCTAGTAGTACAGGCCTGCAACCGGGCCGGCACGGGAC CTTCTTCTCAGGAGATAATCACTACCACCCTGGAGGACGTACCCAGCTACCCTCCCGAAAATGTCCAAGC CATAGCAACCTCACCAGAAAGCATATCAATCTCCTGGTCCACGCTGTCCAAGGAGGCCTTGAATGGGATT CTCCAGGGGTTCAGGGTCATCTACTGGGCCAACCTCATAGACGGAGAGCTGGGCGAGATTAAGAACGTCA CCACCACGCAGCCTTCCTTGGAGTTGGATGGACTAGAGAAATACACCAACTACAGTATCCAGGTGCTGGC CTTCACCCGTGCAGGGGACGGCGTCCGGAGTGAGCAGATCTTTACCCGTACCAAGGAGGATGTTCCAGGT CCTCCTGCTGGTGTCAAGGCAGCAGCAGCCTCGGCCTCCATGGTCTTTGTGTCCTGGCTGCCCCCGCTCA AGCTGAATGGCATCATTCGGAAGTACACGGTGTTCTGCTCCCACCCCTACCCCACAGTCATCAGCGAGTT TGAAGCCTCCCCTGACTCATTTTCCTACAGAATTCCTAACCTGAGTCGGAATCGACAGTATAGCGTCTGG GTGGTGGCAGTGACTTCCGCCGGAAGAGGCAACAGCAGTGAGATCATCACCGTGGAGCCTCTGGCTAAAG CTCCTGCACGGATCCTCACGTTCAGTGGGACAGTGACTACTCCGTGGATGAAGGACATCGTCTTGCCGTG TAAAGCTGTTGGAGACCCATCACCTGCCGTAAAATGGATGAAGGATAGTAACGGGACGCCCAGCCTGGTG ACGATTGATGGCCGCAGAAGCATCTTCAGCAATGGGAGTTTCGTTATTCGCACGGTGAAGGCCGAGGACT CTGGCTACTACAGCTGTGTTGCCAATAACAACTGGGGATCAGATGAGATCATATTAAATTTGCAAGTACA AGTTCCACCAGATCAGCCTCGGCTCACCGTATCCAAGACGACATCCTCCTCCATCACCCTTTCCTGGCTT CCTGGAGACAATGGGGGCAGCTCCATCAGAGGCTACATCCTGCAATATTCCGAGGACAACAGTGAGCAGT GGGGGAGCTTTCCCATCAGCCCCAGCGAGCGTTCCTACCGCTTGGAAAACCTAAAGTGTGGGACATGGTA TAAGTTCACCCTTACTGCCCAAAATGGAGTGGGACCCGGACGCATAAGTGAAATCATAGAAGCCAAAACT CTGGGGAAAGAACCCCAGTTCTCCAAGGAGCAGGAGCTCTTCGCCAGCATCAATACCACCCGAGTGAGGC TGAATCTGATTGGCTGGAACGACGGCGGCTGTCCAATCACCTCATTCACTCTTGAGTACAGACCCTTCGG GACCACGGTCTGGACCACAGCTCAGCGGACCTCCCTTTCCAAGTCTTACATTCTGTATGACCTCCAAGAA GCCACATGGTATGAACTGCAGATGCGAGTGTGCAACAGCGCCGGCTGCGCAGAGAAGCAAGCCAACTTTG CCACGCTGAACTACGATGGCAGTACAATCCCTCCACTCATTAAGTCAGTTGTCCAAAGCGAAGAAGGGCT GACAACCAACGAAGGGCTCAAGATCCTCGTGACCATCTCCTGCATCCTGGTCGGGGTTCTGCTGCTCTTT GTGCTTCTGCTGGTGGTGCGAAGGAGACGGAGGGAGCAGAGGCTGAAGAGGCTGAGAGATGCAAAGAGTT TAGCTGAGATGCTCATGAGCAAGAACACACGGACTTCAGATACCTTGAGCAAACAGCAACAGACTCTGAG GATGCACATTGATATACCCAGGGCTCAGCTTTTGATTGAAGAGAGAGACACAATGGAGACCATAGATGAC CGCTCCACAGTCCTGTTAACGGATGCTGACTTTGGGGAGGCAGCCAAACAGAAGTCACTGACGGTGACTC ACACGGTGCATTACCAATCGGTGTCTCAGGCCACCGGGCCCCTAGTGGATGTCTCCGATGCTCGGCCAGG AACGAATCCCACCACCAGGAGGAATGCAAAGGCTGGACCCACAGCAAGAAACCGGTACGCCAGCCAGTGG ACCCTCAACAGACCCCACCCCACCATCTCAGCACACACTCTCACCACAGACTGGAGACTGCCTACCCCCA GGGCTACAGGCTCCGTGGACAAGGAAAGTGACAGCTACAGCGTCAGCCCATCACAAGACACAGACAGAGC AAGGAGCAGCATGGTCTCCACAGAAAGTGCCTCCTCTACCTACGAAGAACTGGCCAGGGCCTACGAGCAC GCCAAGATGGAGGAGCAGCTGAGGCACGCCAAGTTCACCATCACAGAGTGCTTCATATCCGACACGTCCT CCGAGCAGCTGACGGCAGGGACAAACGAGTACACGGACAGTCTGACCTCCAGTACCCCTTCAGAATCGGG GATCTGCAGATTCACTGCGTCTCCCCCCAAACCTCAGGATGGAGGTCGTGTGGTGAACATGGCGGTTCCA AAGGCCCATCGGCCAGGCGACCTCATACACCTGCCTCCATACCTGCGAATGGACTTCTTGTTAAACCGGG GCGCACCAGGCACCAGCAGGGACCTGAGCTTAGGACAAGCGTGCTTGGAACCCCAGAAAAGTCGGACCCT GAAGCGCCCCACGGTCCTTGAGCCCACCCCTATGGAGGCTTCCTCCTCCACCTCTTCCACGAGAGAAGGA CAGCAGTCGTGGCAACAAGGGGCCGTGGCCACCTTACCTCAGCGAGAGGGTGCAGAGCTGGGACAGGCAG CTAAAATGAGCAGCTCCCAAGAGTCGCTGCTGGACTCTCGGGGCCACTTGAAAGGAAACAATCCCTACGC AAAATCCTACACCTTGGTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_133587
Insert Size	6042 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_133587.1, NP_598271.1
RefSeq Size	6656 bp
RefSeq ORF	6042 bp
Locus ID	171119
UniProt ID	Q8VHZ8
Gene Summary	mouse homolog is a neural cell adhesion molecule; plays a role in axon guidance [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.