Dysf (NM_001107869) Rat Untagged Clone

CAT#: RN209414

Dysf (untagged ORF) - Rat dysferlin (Dysf), (10 ug)



  "NM_001107869" in other vectors (1)

CNY 39,050.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN209414 representing NM_001107869
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTCTGCTGCCTGCTGGCAAGGGCCAGCAACCTCCCCAATGTGAAGAAGGACCGGCGCAGTGACCCTG
TTGCCAGCCTGATTTTTCGAGAAGCCAAGATCCCACTCCAGGAGGTCCTTGCCACCCCCAGCCTATCTGC
CAGCTTCAACGCACCTCTGCTGGACTCCAAGCAGCAACCCACAGGGGCCTCTCTGGTCCTGCAGGTATCC
TACACACCGCCCCCAGGAGCTGCGCCCTTGTTCCCACCACCCGCTTCCCTGGCACCGTCCCCAACACTGC
CTGACATGGACCTGGTGGCTGACACAGGAGGAGAGGAAGACACGGAAGACCAGGGCCTGACTGGAGATGA
AGCTGAGCTCTTCCCAGATCCGAGTGTTGCTGTGGGCCCAGGGGGACCAACGACGCCCAAGAAGCCACCT
TCCCATCCTCCTCCCCACTATCCAGGGGGGAAAAGGAAAAGAAGCTCTGCTCCACCTAGAAAGCTGCTGT
CAGACAAGCCACAGGACTTCCAGATCAGAGTCCAGGTGATTGAGGGGCGTCAGCTGCCTGGGGTGAACAT
CAAGCCTGTGGTCAAGGTCACAGCTGCTGGGCAGACCAAGCGGACTCGAATCCAGAAGGGAAATAGCCCA
CTTTTCAATGAGACTCTTTTCTTCAACGTATTTGACTCTCCCTTGGAACTGTTTGATGAGCCCATCTTCA
TCACGGTGGTAGACTCACGCTCCCTCAGGACAGATGCCCTCCTTGGCGAGTTCCGGATGGATGTGGGCAC
CGTGTACAGAGAGCCCCGACATGCTTATCTCCGGAAGTGGCTGCTGCTCTCCGATCCCGATGACTTCTCT
GCAGGGGCCAGAGGCTACCTCAAAGCCAGCCTGTGTGTGCTGGGGCCTGGGGACGAAGCTCCCCTAGACA
AGAAAGACCCTTCTGAAGACAAAGAGGACATTGAAGGCAACCTGCTTAGGCCCACTGGTGTGGCCCTTCG
TGGTGCCCATTTTTGCCTGAAGCTCTTCCGGGCTGAGGACTTACCACAGATGGACGATGCTGTGATGGAC
AACGTCAAGCAAATCTTTGGCTTTGATAGCAACAAGAAGAACCTGGTGGACCCCTTTGTGGAAGTCAGCT
TTGCTGGGAAAATGCTTTGCAGCAAGATCCTGGAGAAGACTGCTAACCCTCAGTGGAACCAGAACATCAC
ATTGCCTGCGATGTTTCCCTCTATGTGTGAAAAAATGAGGATTCGTGTCATGGACTGGGACCGCCTCACT
CACAATGACACTGTGGCTACCACCTACTTGGGTATGTCGAAAATCTCTGCCACTGGAGGAGAAATAGAAG
AGGAGCCTGCAGGTGTACTCAAGAGTCCAGCCACTGACCTGGATGACAACCTGGGCTTCCTCCCCACTTT
TGGGCCCTGCTATGTGAACCTCTATGGCAGTCCCCGAGAGTTCACGGGCTTCCCAGATCCTTATGCGGAG
CTCAACACAGGAAAGGGAGAAGGTGTGGCATATAGGGGCAGGCTTCTGTTGTCCCTGGAGACCAAACTGG
TGGAGCACAGTGAACAGAAGGTGGAAGACCTTCCTGCTGATGACATCTTGAGAGTGGAGAAGTACCTGCG
GAGGCGCAAATATTCCCTCTTTGCTGCCTTCTACTCAGCCACAATGCTGCAGGATGTGGATGATGCCATC
CAGTTCGAGGTCAGCATTGGGAACTACGGAAACAAGTTTGACACCACCTGCTTGCCGTTGGCCTCTACCA
CCCAATACAGCCGGGCAGTCTTTGATGGATGCCACTACTATTATTTGCCCTGGGGCAACGTGAAGCCTGT
GGTAGTACTGTCCTCATACTGGGAAGACATCAGCCATCGAGTCGAGACTCAGAACCAGCTCCTCAGGATC
GCTGACCGACTGGAAGCTAACCTGGAGCAAGTCCACCTGGCTCTGAAGGCACAGTGTTCCTCTGAGGATG
TGGACGCCCTGGTGGCTCAGTTGACAGATGAGCTCCTTGCAGACTGCAGCCAGCCCCTGTGTGACATCCA
TGAAATACCTTCTGCCACCCACCTTGATCAGTACCTGTTCCGCCTCCGCTCCCGTCACCTGAGCCAGATC
AAAGAAGCTGCCCTGGCCTTGAAGCTTGGCCACAGTGAGCTCTCCACTGCCCTGGAGCAGGCCGAGGACT
GGCTTCTGCATCTCCGTGCCCTGGCTGAGGAGCCCCAGAACAGCCTGCCAGATATCATCATCTGGATGCT
GCAGGGTGACAAGCGTGTGGCCTACCAACGGGTGCCTGCCCATGAAGTCCTCTTCTCCCGGCGGGGCCCC
AGCTACTGCGGCAGGAACTGTGGGAATCTGCAGACCATCTTTCTGAAATATCCTATGGAAGGCATGCCTG
GGCCCCGGATGCCGGTGCAGATCCGGATCAAGCTCTGGTTTGGGCTCTCAGTGGATGAGAAGGAATTCAA
TCAGTTTGCAGAGGGGAAGCTCTCTGTCTTTGCTGAAACGTATGAGAACCAGACAAAGCTGGCGCTGGTC
GGGAACTGGGGCACGACGGGCCTCACCTACCCCAAGTTTTCTGACGTCACAGGCAAGATCAAACTACCGA
AGGATAGCTTCCGCCCCTCAGCTGGCTGGGCCTGGGCAGGAGACTGGTTCGTGTGTCCAGAGAAGACCCT
GCTCCATGATGCTGACGCCGGTCATCTGAGCTTTGTGGAGGAGGTGTTTGAGAACCAGACTCGGCTACCT
GGAGGCCAGTGGATCTATATGAGTGACAACTACACCGACGTGAATGGGGAGAAGGTGCTTCCAAAGGACG
ATATTGAATGCCCGCTGGGTTGGAAGTGGGAAGATGAAGAATGGTCCACAGACCTCAATCGGGCTGTTGA
TGAACAAGGCTGGGAGTACAGCATCACCATACCCCCAGACCGAAAGCCCAAACACTGGGTCCCTGTTGAG
AAGATGTATTACACTCACAGGCGTCGACGCTGGGTCCGACTGCGCAGGAGGGACCTCAGCCAGATGGAGG
CACTGAAGAAGCACAGGCAGGCAGAGGCTGAGGGCGAGGGCTGGGAATACGCCTCTCTCTTTGGCTGGAA
GTTCCACCTGGAGTACCGCAAGACAGATGCCTTTCGCCGCCGCCGCTGGCGCCGCCGCATGGAGCCACTG
GAGAAGACGGGGCCTGCAGCGGTGTTTGCCCTTGAGGGGGCCCTGGGCGGCATGGTGGATGACAAGAGCG
AAGATTCCATGTCTGTGTCTACCCTGAGCTTTGGCGTTAACAGACCCACAATCTCCTGCATCTTTGACTA
TGGGAACCGCTACCATCTGCGTTGCTACCTGTACCAGGCCCGGGACCTGCCCGCCATGGACAAAGACTCC
TTCTCTGATCCCTATGCCATTGTCTCCTTCCTGCATCAAAGCCAGAAGACGGTGGTGGAGAAGAACACCC
TGAACCCCACCTGGGATCAAACCCTCATCTTCTATGAGATAGAGATCTTTGGCGAGCCGGCCAGTGTCGC
CGAGCGGCCACCCAGCATTGTGGTGGAGCTGTATGACCATGACACCTATGGCGCTGACGAGTTCATGGGC
CGCTGTATTTGTCAGCCAAGCCTGGAACGGATGCCCCGCCTGGCCTGGTTCCCACTGACCAGGGGCAGCC
AGCCTGCCGGAGAGCTGCTGGCAGCGTTCGAGCTTATCCAGAGAGAGAAGCCAGCCATCCATCACATCCC
TGGCTTTGAGATGCACGAAACTTCAAGGATTTTGGATGAGACGGAGGACACAGACTTGCCTTACCCACCA
CCTCAAAGGGAGGCCAACATCTACATGGTTCCCCAGAACATCAAGCCAGCTCTCCAGCGTACAGCCATCG
AGATCCTGGCCTGGGGCCTTCGGAACATAAAGAGCTATCAGATGGCCAGCGTGTCTTCCCCCAGCCTCGT
GGTGGAGTGTGGTGGGCAGACAGTGCAGTCCTGTGTCATCAGAAACCTTCGGAAGAACCCCAACTTTGAC
GTCTGTACTCTGTTCATGGAAGTGATGTTACCCAGAGAGGACCTCTACTGTCCCCCTATTGTGGTCAAGG
TCATTGATAATCGCCAGTTTGGCCGCAGGCCAGTTGTGGGTCAGTGCACCATTCGCTCCCTGGAGAACTT
CCTGTGTGACCCGTACTCAGCAGAGAGTCCATCCCCGCAAGGTGGCCCAGATGACGTGAGCTTACTCAGC
CCTGGGGAAGACGTGCTCATTGACATTGATGACAAGGAGCCTCTCATCCCTGTCCAGGAGGAGGAGTTCA
TTGACTGGTGGAGCAAATTCTTTGCTTCTATCGGGGAGAGAGAAAAGTGTGGTTCCTACCTGGAGAAGGA
TTTTGACACCCTGAAGGTCTATGACACACAGCTGGAGAATGTGGAGGCCTTTGGAGGCCTGTCCGACTTT
TGTAATACCTTCAAGCTCTACCGGGGCAAGACTCAGGAGGACACAGAAGATCCATCTGTCATCGGAGAAT
TTAAGGGTCTCTTCAAAATTTATCCCCTCCCAGAAGATCCAGCCATCCCTATGCCCCCGAGACAATTCCA
TCAGCTGGCTGCTCAGGGTCCCCAGGAATGCTTGGTCCGGATTTACATCATCCGAGCATTTGGCTTACAG
CCCAAGGACCCCAATGGCAAGTGCGACCCTTACATCAAGATCTCTATAGGGAAGAAATCAGTGAGTGACC
AGGATAACTACATCCCTTGTACCCTGGAGCCTGTATTTGGAAAGATGTTTGAACTCACCTGCACTCTACC
TCTGGAGAAGGACTTGAAGATCACACTCTATGACTATGACCTGCTCTCCAAGGATGAGAAGATTGGGGAG
ACAGTCATTGATCTGGAGAATAGGCTGCTGTCCAAGTTTGGGGCTCGCTGTGGCCTTCCACAGACCTACT
GTGTCTCTGGACCAAATCAGTGGAGAGATCAACTCCGCCCTTCCCAGCTCCTCCACCTCTTCTGCCAGCA
GCACAGGGTCAAGGCCCCCGTGTACCGGACAGACCGAGTGACGTTTCAGGATAAGGAATACACCATTCAG
GAGATAGAGGCTGGCAGACTCCCAAACCCACACCTGGGCCCGGTGGAGGAACGCTTAGCCCTGCATGTCC
TTCAACAACAAGGCTTGGTCCCTGAGCATGTGGAGTCTCGGCCTCTGTACAGTCCTCTGCAGCCAGATAT
TGAGCAGGGGAAGCTACAGATGTGGATTGACATATTTCCAAAGGTGCTGGGCCGGCCTGGACCTCCCTTC
AACATCACCCCACGGAAAGCTCGAAGGTTTTTTCTGCGTTGTATTATTTGGAACACAAAAGATGTGATCT
TGGATGACCTCAGCCTCACGGGGGAGAAGATGAGTGACATCTATGTGAAAGGCTGGATGGTGGGATTCGA
AGAACACAAACAGAAGACAGATGTGCACTACCGTTCCCTGGGCGGGGAGGGCAATTTCAACTGGAGGTTC
GTGTTTCCCTTCGACTATCTGCCCGCTGAGCAAGTCTGTGCTGTTGCCAAGAAGGATGCTTTCTGGAGAC
TCGACAAGACAGAGAGCAAGATCCCAGCACGTGTGGTCTTCCAGATTTGGGACAATGACAAGTTCTCCTT
TGATGACTTTCTGGGCTCCCTGCAGCTGGATCTCAACCGAATGCCCAAGCCAGCCAAGACGGCTGAGAAG
TGCTCCTTGGACCAGCTGGATGACACCTTCCACCCAGAATGGTTTGTGTCCCTTTTTGAGCAGAAGACAG
TGAAAGGATGGTGGCCTTGTGTGACAGAGGAGGGCGCGAAGAAGATCCTGGCGGGGAAGCTGGAAATGAC
CTTGGAGATCGTTGCAGAAAGTGAACATGAGGAGCGGCCTGCTGGCCAGGGTCGGGATGAACCCAACATG
AATCCAAAGCTAGAAGATCCCAGGCGCCCTGACACCTCTTTCCTGTGGTTCACCTCACCATACAAGACCA
TGAAGTTCATCTTGTGGCGACGATTCCGGTGTGCCATCATCCTCTTCATCATCCTCTTCATCCTCCTGCT
CTTCCTGGGTGTCTTTGTCTACGCCTTCCCGAACTATGCTGCCATGAAGCTGGTGAAGCCCTTTAGATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001107869
Insert Size 6090 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001107869.1, NP_001101339.1
RefSeq Size 6319 bp
RefSeq ORF 6090 bp
Locus ID 312492
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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