Atrn (NM_031351) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209486 representing NM_031351 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCGGCGGCGGCGGCGGCGGAGGCGACTGAGGCGCGGTTGAGGGGGTACACGACGGCGACAGCAG CGCCCGCGGGCTGGAAGGAGAGGCAGCACCGACCCTGCGCCGCGACAGGGGCCTGGAGGCCGTGGCCGCG CGCCGGGCTGTGTCTCCCGCGGGTGCTGTCGCGGGCGCTATCCCCGCCGCCGCTGCTGCCGCTGCTGCCG CTGCTCTTTTCGCTATTGCTGCTGCCACTGCCCCGGGAGGCCGAGGCCGCTGCGGTGGCGGCGGCTGTGT CAGGCTCGGCCGCAGCTGAGGCCAAGGAATGTGACCGGCCGTGTGTCAACGGCGGCCGCTGCAACCCTGG CACCGGCCAGTGCGTCTGCCCCACGGGCTGGGTGGGCGAGCAATGCCAGCACTGCGGGGGCCGCTTCAGA CTAACTGGATCTTCTGGATTTGTAACAGATGGACCTGGGAATTATAAATATAAAACGAAATGCACATGGC TCATTGAAGGACAGCCAAATAAGATAATGAGACTTCGATTCAACCATTTTGCTACTGAATGTAGCTGGGA CCATTTATATGTTTATGATGGGGACTCAATTTACGCACCTCTGATTGCTGCCTTTAGTGGCCTCATTGTT CCTGAAAGAGATGGCAATGAGACGGCCCCTGAAGTCACTGTCACTTCAGGTTATGCACTACTGCATTTTT TCAGTGATGCTGCTTATAATCTGACTGGATTTAATATCACTTACAATTTTGACATGTGCCCGAATAATTG CTCAGGCCGAGGAGAATGTAAGAGCAGTAACAGCAGCAGCACTGTTGAGTGTGAATGTTCTGAAAACTGG AAAGGGGAGTCCTGTGACATTCCTCACTGTACAGACAACTGTGGCTTTCCTCACCGAGGCATCTGTAACG CAAGTGACACCAGAGGGTGCTCCTGCTTCCCTCACTGGCAGGGTCCTGGATGTTCAATTCCTGTGCCAGC TAACCAGTCTTTTTGGACTCGAGAAGAATATTCTGACTTAAAGCTTCCCAGAGCCTCTCATAAAGCTGTG GTCAATGGAAATATAATGTGGGTTGTTGGCGGGTATATGTTCAACCATTCAGATTACAGCATGGTTTTAG CGTATGACCTGGCTTCTAGGGAATGGCTTTCACTAAACCATTCTGTGAACAGTGTGGTTGTAAGATATGG TCATTCTTTGGCATTACATAAGGATAAAATCTACATGTATGGAGGAAAAATTGATTCAACAGGGAATGTG ACCAACGAGCTGAGAGTATTTCATATTCATAATGAATCATGGGTATTGTTAACCCCCAAAGCTAAGGATC AGTATGCAGTGGTTGGACACTCAGCACACATTGTTACACTGTCATCTGGCCGTGTGGTCATGTTGGTCAT CTTCGGTCATTGTCCGCTCTACGGATATATAAGTGTTGTGCAGGAATATGACTTGGAAAAGAACACATGG AGTATATTACAAACTCAAGGTGCTCTAGTGCAAGGGGGTTATGGCCACAGCAGTGTTTATGATCACAGGA CCAAGGCTCTGTATGTTCATGGTGGCTACAAGGCTTTCAGCGCCAACAAGTACCGGCTTGCAGATGACCT CTACAGATACCATGTGGACACTCAGATGTGGACCATTCTTAAGGACAGCCGATTTTTCCGTTACTTGCAT ACAGCTGTGATAGTGAGTGGAACCATGCTGGTGTTCGGAGGGAACACACACAATGACACTTCCATGAGCC ACGGTGCCAAATGCTTCTCCTCAGACTTCATGGCTTATGACATTGCTTGTGACCGATGGTCAGTTCTCCC CAGACCTGAGCTCCATCACGATGTCAACCGATTTGGCCACTCAGCAGTCTTGCACAACAGCACCATGTAT GTGTTCGGCGGCTTCAACAGCCTCCTCCTCAGTGACGTCTTAGTCTTCACCTCGGAGCAGTGTGATGCAC ACCGCAGCGAAGCTGCTTGTGTGGCAGCAGGACCTGGCATCCGGTGTCTGTGGGACACACAGTCGTCTCG ATGTACCTCCTGGGAGTTGGCAACTGAAGAACAAGCAGAAAAGTTAAAATCAGAATGTTTCTCTAAAAGA ACCCTTGACCATGACAGATGTGACCAGCACACAGATTGTTACAGCTGCACAGCCAATACCAATGACTGCC ACTGGTGCAATGATCACTGTGTCCCTGTGAACCACAGCTGCACAGAAGGCCAGATCTCCATTGCCAAGTA TGACAATTGCCCCAAGGATAACCCCATGTACTACTGCAATAAGAAAACCAGCTGCAGGAGCTGTGCCCTA GATCAGAATTGCCAGTGGGAACCTCGAAATCAAGAGTGCATCGCCCTGCCCGAAAACATCTGTGGCATTG GCTGGCATTTGGTCGGAAACTCGTGTCTGAAAATCACTACTGCTAAGGAAAATTATGACAATGCTAAATT GTCCTGTAGAAACCACAATGCCTTTTTGGCTTCGCTTACGTCCCAGAAGAAGGTGGAGTTTGTCCTTAAG CAGCTTCGATTAATGCAATCATCTCAGAGCACGTCCAAGCTCACTCTGACTCCATGGGTTGGTCTTCGGA AGATCAATGTATCTTACTGGTGCTGGGAGGATATGTCTCCATTCACAAATAGTTTGCTGCAGTGGATGCC ATCTGAGCCCAGTGATGCCGGCTTCTGTGGGATCTTGTCAGAGCCTAGTACTCGGGGACTGAAGGCTGCA ACCTGCATCAACCCACTCAATGGAAGTGTTTGTGAAAGGCCTGCAAACCACAGTGCCAAGCAGTGCCGGA CACCCTGTGCCCTGCGGACAGCATGTGGTGAGTGCACCAGCAGCAGCTCCGAATGCATGTGGTGCAGCAA CATGAAGCAGTGCGTAGACTCCAATGCCTACGTAGCCTCCTTTCCTTTCGGCCAGTGTATGGAGTGGTAT ACAATGAGCAGCTGCCCACCTGAAAATTGCTCTGGCTACTGTACCTGCAGCCATTGCTTGGAGCAGCCAG GCTGTGGTTGGTGTACTGATCCTAGCAATACTGGGAAAGGCAAATGTATTGAGGGAAGCTATAAAGGACC CGTGAAGATGCCTTCACATGCCTCTACAGGAAATGTGTACCCACAGCCCCTTCTGAACTCCAGCATGTGT CTAGAGGACAGCAGATACAACTGGTCTTTTATTCACTGTCCAGCTTGCCAGTGCAATGGACACAGCAAGT GCATCAACCAGAGCATCTGTGAGAAGTGTGAGGACCTGACCACAGGCAAGCACTGCGAGACCTGCATATC TGGCTTCTATGGTGACCCGACTAATGGAGGCAAATGTCAGCCATGCAAGTGCAACGGGCACGCATCACTG TGCAACACCAACACTGGCAAGTGCTTCTGTACCACCAAGGGCGTCAAGGGAGAGGAGTGCCAGCTATGTG AGGTAGAAAATCGATACCAGGGAAACCCTCTCAAAGGAACATGTTACTATACCCTTCTCATTGACTATCA GTTCACTTTTAGCCTGTCCCAGGAAGACGACCGCTATTACACAGCCATCAATTTTGTGGCTACTCCTGAT GAACAAAACAGGGATTTGGACATGTTCATCAATGCCTCCAAAAACTTCAACCTCAACATCACCTGGGCCA CCAGTTTCCCAGCTGGAACCCAGACTGGAGAAGAGGTGCCTGTTGTTTCAAAAACCAACATCAAGGAATA CAAAGATAGCTTCTCTAATGAGAAATTTGATTTTCGCAACCATCCAAACATTACTTTCTTTGTTTATGTC AGTAATTTCACTTGGCCCATCAAAATTCAAATTGCCTTCTCCCAGCACAGCAATTTTATGGACCTGGTGC AGTTCTTCGTGACTTTCTTCAGCTGTTTCCTCTCTCTGCTTCTGGTGGCGGCAGTGGTCTGGAAGATCAA GCAGAGCTGTTGGGCATCCAGGAGGAGAGAGCAACTTCTTCGGGAGATGCAACAGATGGCCAGCCGCCCT TTTGCTTCTGTAAATGTTGCCTTGGAAACAGATGAAGAACCTCCTGATCTCATTGGGGGAAGTATAAAGA CTGTTCCTAAGCCCATTGCCCTGGAGCCCTGCTTTGGTAACAAAGCTGCTGTCCTCTCTGTGTTTGTGAG GCTCCCTCGAGGACTGGGAGGAATCCCTCCTCCTGGTCAGTCAGGTCTTGCTGTGGCCAGTGCCCTGGTG GACATTTCTCAGCAGATGCCAATAGTGTACAAGGAGAAGTCAGGAGCTGTAAGGAACCGGAAGCAGCAGC CCCCTGCACAGCCTGGAACCTGCATTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031351
Insert Size	4299 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031351.1, NP_112641.1
RefSeq Size	8739 bp
RefSeq ORF	4299 bp
Locus ID	83526
UniProt ID	Q99J86
Gene Summary	gene with an important role in enhancing cell survival against oxidative stress in the brain; mutations in gene result in the zwitter (zi) rat phenotype and the myelin vacuolation (mv) rat phenotype [RGD, Feb 2006]

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