Fn1 (NM_019143) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	FIBNEC; fn-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN209525 representing NM_019143 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAGGGGTCCGGGACCCGGGCGGCTGCTGCTGCTAGCAGTCCTGTGCCTGGGGACATCGGTGCGCT GCACCGAAACCGGGAAGAGCAAGAGGCAGGCTCAGCAAATCGTGCAGCCTCCGTCCCCGGTGGCTGTCAG TCAGAGCAAGCCTGGCTGTTTTGACAACGGGAAGCATTATCAGATAAATCAGCAGTGGGAACGGACCTAC CTAGGCAACGCCCTGGTTTGTACCTGCTATGGAGGAAGCAGAGGTTTTAACTGCGAGAGCAAGCCTGAAC CTGAAGAGACCTGTTTTGACAAATACACTGGAAACACTTACAAAGTGGGTGACACTTATGAGCGCCCTAA AGATTCCATGATCTGGGACTGTACCTGCATTGGGGCTGGGCGAGGCAGGATCAGCTGTACCATTGCAAAT CGCTGCCATGAAGGGGGTCAGTCCTACAAGATTGGTGACAAGTGGAGGAGGCCACATGAGACTGGTGGCT ATATGTTGGAGTGTTTGTGTCTGGGGAATGGAAAAGGGGAATGGACCTGCAAGCCAATAGCTGAGAAATG TTTTGATCACGCTGCTGGGACTTCCTACGTCGTGGGGGAGACCTGGGAAAAGCCCTACCAAGGCTGGATG ATGGTGGACTGTACTTGTCTGGGCGAAGGCAATGGGCGTATCACCTGCACCTCCCGGAACAGATGCAATG ATCAGGACACCAGGACGTCCTACAGAATTGGAGACACATGGAGCAAGAAGGACAACAGAGGGAACCTGCT CCAGTGTGTCTGCACAGGCAACGGCAGAGGGGAGTGGAAGTGTGAGCGACATGTTCTACAGAGTGCTTCA GCTGGATCTGGCTCCTTCACAGATGTCCGAACAGCTATTTACCAACCCCAGACCCACCCCCAGCCCGCAC CGTACGGCCACTGTGTCACAGACAGCGGTGTGGTCTACTCTGTGGGAATGCAGTGGCTGAAGTCTCAAGG AGACAAGCAGATGCTGTGCACTTGCCTGGGCAATGGCGTCAGCTGCCAGGAGACAGCTGTGACCCAGACT TACGGTGGCAACTCAAACGGGGAGCCCTGTGTTCTCCCGTTCACCTACAACGGTAGGACCTTCTACTCCT GCACCACCGAAGGGCGGCAAGACGGACATCTGTGGTGTAGCACAACTTCAAATTATGAACAAGACCAGAA GTATTCTTTCTGCACAGACCACGCGGTTTTGGTTCAGACTCGAGGTGGGAATTCCAATGGTGCCTTGTGC CACTTCCCCTTCCTGTACAACAACCGGAATTACACCGACTGTACTTCTGAGGGTAGGCGGGACAACATGA AATGGTGCGGCACCACCCAGAACTACGATGCCGATCAGAAGTTTGGATTCTGCCCAATGGCTGCCCATGA GGAGATCTGCACGACCAACGAAGGGGTCATGTATCGCATTGGGGACCAGTGGGATAAGCAGCATGACCTG GGCCACATGATGAGGTGCACGTGTGTTGGGAACGGCCGTGGAGAATGGGCCTGCATCCCCTACTCCCAGC TCCGAGATCAGTGCATCGTTGATGACATTACTTACAACGTCAACGACACGTTCCACAAGCGTCACGAGGA GGGACATATGCTGAACTGTACCTGCTTCGGTCAGGGCCGGGGCAGATGGAAATGTGACCCCATCGACCAG TGCCAAGATTCAGAGACCCGGACATTTTACCAGATTGGTGACTCCTGGGAGAAGTTTGTGCATGGTGTCA GATACCAGTGTTACTGTTACGGCCGTGGCATTGGGGAGTGGCACTGCCAGCCTCTGCAGACCTACCCAGG CACAACTGGACCTGTTCAAGTAATTATCACGGAGACCCCCAGCCAGCCCAATTCCCACCCCATCCAGTGG AATGCCCCGGAGCCTTCACACATCACCAAGTACATTCTCAGGTGGAGACCTAAAACCTCTACGGGTCGCT GGAAGGAAGCTACCATTCCAGGCCACCTTAACTCCTATACCATCAAAGGCCTGACCCCAGGTGTGATCTA CGAGGGACAGCTCATCAGCATCCAGCAGTACGGGCACCAAGAAGTGACTCGCTTTGACTTCACCACCAGC GCCAGCACACCTGTGACCAGCAACACAGTGACTGGAGAGACTGCGCCCTTTTCTCCTGTTGTGGCCACTT CCGAATCTGTCACTGAAATCACAGCCAGCAGCTTCGTGGTCTCCTGGGTCTCAGCTTCCGACACGGTGTC AGGATTCCGAGTGGAGTACGAACTGAGCGAGGAAGGAGATGAGCCTCAGTACCTTGATCTTCCAAGCACA GCCACTTCTGTGAACATTCCTGACCTGCTCCCGGGCAGAAAGTACATCGTCAACGTCTATCAGATATCTG AAGAGGGAAAGCAGAGCTTGATCCTGTCTACATCACAGACTACAGCACCTGATGCGCCTCCAGACCCTAC TGTGGACCAGGTTGATGACACTTCCATTGTTGTTCGATGGAGCAGACCCCAGGCACCTATCACAGGGTAC AGGATTGTCTATTCACCTTCAGTAGAAGGCAGTAGCACAGAACTCAACCTTCCTGAAACGGCCAACTCCG TCACCCTCAGCGACCTGCAGCCCGGTGTTCAGTACAACATCACTATCTATGCTGTGGAGGAGAACCAGGA GAGCACACCCGTTTTCATCCAGCAGGAGACTACTGGCGTCCCACGATCCGATGATGTTCCCGCTCCAAAG GACCTACAGTTTGTGGAAGTGACCGACGTGAAAGTCACCATCATGTGGACACCTCCTAATAGCGCAGTGA CTGGATACCGTGTGGATGTCCTGCCTGTCAACCTGCCAGGGGAACATGGGCAGAGGCTGCCTGTCAACAG GAACACCTTTGCTGAAGTCACCGGACTGTCCCCAGGGGTCACGTACCTCTTCAAAGTCTTTGCTGTGCAT CAGGGCAGGGAAAGCAAGCCTCTGACAGCACAACAGACCACCAAACTCGATGCTCCCACTAACCTCCAGT TTGTCAATGAAACGGACAGAACAGTTCTGGTAACTTGGACTCCACCTCGAGCCCGGATAGCAGGCTACCG ACTGACAGTGGGCCTCACCCGAGGAGGCCAGCCCAAGCAGTACAATGTGGGACCCATGGCTTCCAAGTAT CCCCTGAGAAATCTGCAGCCTGGGTCTGAGTACACTGTGACCTTGATGGCTGTGAAAGGCAACCAGCAGA GTCCCAAAGCCACCGGAGTCTTTACTACCCTGCAGCCTCTGCGCTCCATTCCACCTTATAACACCGAGGT GACAGAGACCACAATCGTGATCACCTGGACCCCCGCTCCAAGGATTGGCTTCAAGCTGGGTGTACGACCA AGCCAGGGAGGTGAAGCACCCCGAGAAGTGACTTCAGACTCAGGAAGCATCGTTGTGTCTGGCTTGACTC CAGGCGTGGAATACACGTACACCATCCAAGTCCTGAGGGACGGCCAGGAGAGAGATGCACCAATTGTCAA CCGAGTAGTGACACCGCTGTCTCCCCCAACCAACTTGCACCTGGAGGCCAATCCTGACACTGGAGTGCTT ACCGTCTCCTGGGAGAGGAGCACCACCCCAGATATTACTGGCTACAGAATAACCACCACCCCCACAAACG GGCAGCAGGGAACCGCTTTGGAAGAAGTGGTTCATGCCGATCAGAGTTCCTGCACTTTTGAAAACCTGAA TCCTGGCCTGGAGTACAATGTCAGTGTTTACACTGTCAAAGATGACAAGGAAAGTGCCCCTATCTCTGAT ACCGTCATCCCAGAGGTGCCCCAGCTCACTGACCTAAGCTTTGTTGATATAACTGACTCAAGCATCGGCC TGAGGTGGACCCCGCTAAACTCTTCCACCATTATCGGGTACCGAATCACAGTAGTTGCGGCAGGAGAAGG GATCCCCATTTTTGAAGATTTTGTGGACTCCTCAGTAGGATACTACACAGTTACAGGGCTGGAACCCGGC ATTGACTATGACATCAGCGTTATCACTCTCATTAATGGCGGAGAGAGTGCCCCTACTACACTGACACAGC AAACGGCCGTCCCTCCTCCCACGGATCTGCGATTCACCAATATCGGTCCGGACACTATGCGGGTCACTTG GGCCCCGCCTCCGTCCATTGAGCTAACCAACCTCTTGGTGCGCTACTCACCTGTGAAGAACGAGGAGGAT GTGGCAGAGCTGTCCATTTCACCCTCAGACAACGCCGTGGTCCTAACAAATCTCCTGCCTGGGACTGAGT ACTTAGTCAGTGTCTCCAGCGTGTACGAACAGCATGAGAGCATCCCTCTCAGAGGAAGACAGAAAACAGG TCTGGACTCCCCAACTGGTTTTGATTCTTCTGATGTCACCGCCAACTCATTCACCGTCCACTGGGTGGCT CCTCGGGCCCCCATCACCGGCTACATCATCCGCCATCACGCCGAGCATTCTGCCGGAAGACCCAGGCAAG ACCGAGTGCCGCCCTCAAGGAATTCTATCACCCTCACCAACCTTAATCCGGGCACGGAGTACATTGTCAC CATCATTGCTGTTAATGGCAGAGAGGAGAGCCCCCCACTGATTGGCCAGCAATCCACGGTTTCCGATGTC CCGAGAGATCTGGAGGTCATCGCTTCCACCCCCACCAGCCTGCTCATCAGTTGGGAACCCCCCGCCGTCT CTGTGCGCTATTACAGAATCACCTATGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAATTCACTGTGCC CGGAAGCAAGTCCACCGCCACCATCAACAACATTAAACCAGGAGCAGACTACACCATCACCCTGTATGCT GTCACTGGCCGTGGGGACAGTCCAGCCAGCAGCAAGCCAGTTTCCATCAATTATCAAACAGAAATTGACA AGCCATCCCAGATGCAGGTGACGGATGTCCAGGACAACAGCATCAGTGTCAGGTGGCTGCCTTCAACTTC TCCTGTGACAGGTTACAGAGTGACCACCGCTCCCAAAAATGGCCTAGGACCAACAAAATCTCAAACTGTC AGTCCAGATCAAACAGAAATGACCATTGAAGGTTTGCAACCCACCGTGGAGTATGTGGTTAGTGTCTATG CTCAGAACCGGAACGGAGAAAGCCAGCCCCTGGTTCAGACTGCAGTGACCAACATTGACCGCCCTAAAGG ACTGGCATTCACTGATGTGGATGTCGATTCCATCAAAATTGCCTGGGAAAGCCCACAGGGGCAAGTTTCC AGGTACAGGGTGACCTACTCAAGCCCTGAGGATGGAATCCATGAGCTTTTCCCTGCGCCTGATGGTGACG AGGACACGGCAGAGCTGCACGGCCTCAGGCCGGGTTCTGAGTACACAGTCAGTGTGGTTGCCTTGCACGG TGGCATGGAGAGCCAGCCCCTGATTGGAGTCCAGTCCACAGCCATTCCTGCGCCAACCAATCTGAAGTTC ACTCAGGTGTCACCCACCACCTTGACTGCCCAGTGGACAGCACCCAGTGTTAAGCTCACTGGCTACCGAG TGCGGGTGACCCCGAAGGAGAAGACAGGACCAATGAAAGAAATCAACCTTTCTCCAGACAGCACCTCCGT GATTGTGTCAGGGCTCATGGTGGCCACCAAGTATGAAGTCAGCGTCTATGCTCTCAAGGACACATTGACA AGCAGACCAGCTCAGGGAGTCGTCACGACTCTGGAGAATGTCAGCCCTCCAAGAAGGGCCCGTGTGACCG ACGCTACAGAAACTACCATCACTATTAGCTGGAGAACGAAGACAGAGACGATCACTGGCTTCCAAGTCGA TGCCATTCCAGCCAATGGCCAGACCCCGGTTCAGAGGACCATCAGCCCGGATGTCAGAAGCTATACTATT ACAGGTTTACAGCCAGGCACTGACTACAAGATCCACCTGTACACGCTCAACGACAATGCCCGGAGCTCTC CTGTGGTCATTGATGCCTCCACGGCCATTGATGCCCCATCCAACCTGCGGTTCCTGACCACCACACCCAA CTCCTTGCTGGTATCATGGCAGGCACCCCGTGCCAGGATTACTGGCTACATTATCAAGTATGAGAAGCCT GGATCCCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACGGAGGCCACCATCACTGGTCTGG AGCCAGGAACCGAGTACACCATCTATGTCATCGCACTGAAGAACAATCAGAAGAGTGAGCCCCTGATTGG GAGGAAAAAGACAGATGAGCTTCCCCAACTGGTTACCCTTCCACACCCCAATCTTCATGGACCAGAGATC TTGGATGTTCCCTCCACAGTTCAAAAGACCCCCTTCGTCACCAACCCTGGGTATGACACCGAAAATGGTA TTCAGCTTCCTGGCACATCCCACCAACAACCCAGTGTTGGGCAACAAATGATCTTTGAGGAACATGGCTT TAGGCGAACCACGCCACCCACTGCGGCCACCCCCGTCAGGCTTAGGCCAAGACCATACCTGCCGAATGTA GATGAGGAGGTCCAAATCGGTCATGTTCCCAGGGGAGACGTAGACTACCACCTCTATCCTCATGTTCCGG GGCTCAATCCAAATGCCTCTACAGGACAAGAAGCTCTCTCTCAGACAACCATCTCTTGGACGCCATTCCA GGAGAGTTCTGAGTACATCATTTCATGCCAACCTGTTGGCACTGACGAAGAGCCCTTACAGTTCCAAGTT CCTGGAACTTCTACCAGTGCGACTCTGACTGGCCTTACCAGAGGGGTCACCTACAACATCATAGTGGAGG CCCTGCACAACCAGAGGAGGCACAAGGTCCGAGAAGAGGTTGTTACTGTAGGCAACACTGTCAACGAAGG CCTGAACCAGCCTACGGATGACTCATGCTTTGACCCTTACACGGTTTCCCATTACGCCGTTGGAGAGGAA TGGGAGCGGTTATCTGACTCTGGCTTTAAGCTCACTTGCCAGTGCTTGGGCTTTGGCAGTGGTCATTTCA GATGCGATTCATCTAAATGGTGCCATGACAACGGTGTCAACTACAAGATCGGAGAGAAGTGGGATCGTCA GGGAGAAAATGGCCAGCGGATGAGCTGCACATGTCTCGGGAATGGAAAGGGAGAATTCAAATGCGATCCC CATGAAGCAACGTGTTATGACGACGGGAAGACCTACCACGTAGGAGAACAGTGGCAGAAAGAGTATCTCG GAGCCATTTGCTCCTGCACGTGTTTCGGGGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGCAGACCTGG GGCTGCTGAACCCAGTCCCGATGGTACCACTGGCCACACCTACAACCAGTATACACAGAGATACCATCAG AGAACGAACACTAATGTAAATTGCCCAATTGAATGCTTCATGCCGTTGGACGTGCAGGCTGACAGAGATG ATTCCAGAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019143
Insert Size	7434 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019143.2, NP_062016.2
RefSeq Size	8331 bp
RefSeq ORF	7434 bp
Locus ID	25661
Gene Summary	extracellular matrix component; may play a role in fibrosis and tumor metastasis [RGD, Feb 2006]

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