Cry2 (NM_133405) Rat Untagged Clone

CAT#: RN210144

Cry2 (untagged ORF) - Rat cryptochrome 2 (photolyase-like) (Cry2), (10 ug)



  "NM_133405" in other vectors (3)

CNY 6,648.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN210144 representing NM_133405
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCTGCTGTGGTGGCAGCGACGGTCCCCGTGCAGTCGATGGGCGCGGACGGCGCGTCCTCCG
TGCACTGGTTCCGCAAAGGACTACGGCTCCACGACAACCCCGCGCTGTTAGCTGCCGTGCGCGGGGCGCG
CTGTGTGCGCTGCGTCTACATCCTCGACCCGTGGTTCGCGGCCTCCTCGTCAGTGGGCATCAACCGATGG
AGGTTCCTACTGCAGTCTCTAGAAGATCTGGACACAAGCTTAAGAAAGCTGAATTCCCGTCTGTTTGTAG
TCCGGGGTCAGCCAGCTGATGTGTTCCCAAGGCTTTTCAAGGAATGGGGGGTGACCCGCTTGACCTTTGA
ATATGACTCCGAACCCTTTGGGAAAGAACGGGATGCAGCCATTATGAAGATGGCCAAGGAGGCGGGTGTG
GAGGTGGTGACTGAGAACTCTCACACCCTTTATGACTTAGACAGAATCATCGAACTGAATGGGCAGAAAC
CACCCCTTACCTACAAGCGCTTTCAGGCTCTCATCAGCCGTATGGAGCTGCCCAAGAAGCCAGTGGGGGC
TGTGAGCAGCCAGCATATGGAGAACTGCAGAGCTGAGATCCAGGAGAACCATGATGACACCTATGGCGTG
CCTTCCTTAGAGGAACTGGGATTCCCCACAGAAGGACTTGGCCCAGCTGTTTGGCAAGGAGGAGAGACAG
AAGCTCTGGTCCGCCTGGATAAGCACTTGGAACGGAAGGCCTGGGTTGCCAACTATGAGAGACCTCGGAT
GAATGCCAATTCCTTGCTGGCCAGCCCCACAGGCCTCAGCCCCTACCTGCGCTTTGGCTGCCTCTCCTGC
CGCCTCTTCTACTACCGCCTGTGGGACTTGTACAGAAAGGTGAAGAGGAACAGCACACCCCCCCTCTCCT
TATTTGGACAACTCCTATGGCGAGAATTCTTCTATACAGCGGCCACCAACAACCCCAGGTTTGACCGAAT
GGAGGGGAACCCCATCTGCATCCAGATCCCCTGGGACCGAAACCCCGAAGCCCTGGCCAAGTGGGCCGAG
GGCAAGACAGGCTTCCCTTGGATTGACGCCATCATGACCCAACTGAGGCAGGAGGGCTGGATCCACCACC
TGGCCCGGCACGCTGTGGCCTGCTTCCTCACCCGAGGGGACCTCTGGGTCAGCTGGGAGAGCGGGGTCCG
GGTATTTGATGAGTTGCTCCTGGATGCAGATTTCAGCGTGAATGCAGGCAGCTGGATGTGGCTGTCCTGC
AGTGCTTTCTTCCAACAGTTCTTCCACTGCTACTGCCCTGTGGGCTTTGGCCGACGCACGGACCCCAGTG
GGGACTACATCCGGCGATACCTGCCCAAACTGAAAGGCTTCCCCTCTCGATATATCTATGAGCCCTGGAA
TGCTCCCGAGTCGGTTCAGAAGGCCGCTAATTGCATCATTGGCGTGGACTACCCACGGCCCATCGTTAAC
CACGCAGAGACTAGTCGGCTCAACATTGAGCGGATGAAGCAGATCTACCAACAGCTGTCACGATACCGGG
GGCTCTGTCTGTGGGCATCTGTCCCTTCCTGTGTAGAAGACCTCAGTCACCCTGTGGCAGAGCCTGGTTC
TAGCCAGGCTGGGAGCATCAGCAACACAGGCCCCAGACCACTGTCCAGTGGCCCAGCCTCCCCCAAACGC
AAGCTGGAAGCAGCTGAGGAACCTCCAGGTGAAGAACTGAGCAAGCGGGCTAGAGTGACAGTGACTCAGA
TGCCTGCCCAGGAGCCACCAAGCAAGGACTCCTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_133405
Insert Size 1785 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_133405.1, NP_596896.1
RefSeq Size 3988 bp
RefSeq ORF 1785 bp
Locus ID 170917
Gene Summary This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. [provided by RefSeq, Feb 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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