F7 (NM_152846) Rat Untagged Clone

CAT#: RN210297

F7 (untagged ORF) - Rat coagulation factor VII (serum prothrombin conversion accelerator) (F7), (10 ug)



  "NM_152846" in other vectors (3)

CNY 5,700.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN210297 representing NM_152846
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTCCACAGACTCACGGACTGCTGCTTCTCTACTTTCTGCTCCAGCTCCAGGGACCCCTAGGGGCTG
TGGTTTTCATAACCCAGGAGGAAGCACACGGTGTCCTACACAGGCAAAGGCGTGCCAACTCACTCCTAGA
GGAGCTTTGGTCTAGCTCCTTGGAGAGGGAGTGCAATGAAGAGCGGTGCTCCTTTGAGGAGGCCCGAGAG
ATCTTCAAGAGCCCTGAGAGAACCAAGCAGTTCTGGACTATTTACAGCGATGGCGACCAGTGTGCCTCGA
ATCCATGTCAGAACGGGGGTACCTGCCAGGATCACCTCAAGTCTTATGTCTGCTTCTGCCCCCTAGACTT
TGAGGGCCGGAACTGTGAGAAAAACAAGAATGAGCAGCTGATCTGTGCAAATGAAAATGGTGACTGTGAC
CAGTACTGCAGGGACCACGTAGGGACCAAGCGTACCTGTAGCTGTCACGAGGACTACGTGCTGCAGCCAG
ATGAGGTGTCCTGCAAACCAAAAGTTGAGTACCCATGCGGGAGAATACCTGTTGTAGAAAAAAGAAACTT
CAGCAGACCCCAAGGCCGGATTGTGGGAGGCTATGTGTGCCCCAAAGGGGAGTGCCCATGGCAGGCTGTG
CTGAAATTCAATGAGGCATTGCTGTGTGGGGCCGTCCTGCTGGACACCAGATGGATAGTAACTGCAGCCC
ACTGCTTCGATAAATTCGGGAAATTGGTAAACATCACAGTGGTGTTGGGTGAACACGACTTCAGTGAGAA
GGAGGGGACTGAGCAAGTACGGCTGGTGGAACAGGTCATCATGCCCAACAAGTACACCCGCGGCAGGACT
GACCATGACATCGCCCTGGTCCGCCTTCACCGGCCTGTAACCTTCACTGACTACGTGGTACCTCTGTGTC
TGCCTGAACGGGCCTTCTCCGAGAACACCCTAGCCAGCATCCGCTTCTCGAGGGTCAGCGGCTGGGGCCA
GCTACTGGACCGTGGTGCCACAGCTCTGGAGCTCATGGTCATCGAGGTGCCCCGGCTGATGACCCAGGAC
TGCCTGGAGCATGCCAAACACAGTGCTAACACCCCCAGAATCACGGAGAACATGTTCTGCGCCGGCTACA
TGGACGGCACCAAGGACGCCTGCAAGGGTGACAGTGGAGGCCCACACGCCACCCACTACCATGGCACTTG
GTATCTGACAGGTGTGGTCAGCTGGGGGGAGGGCTGTGCAGCTATCGGCCACATCGGGGTGTACACCAGG
GTCTCCCAGTACATAGACTGGCTGGTCAAATACATGGACTCCAAGCTCCGGGTTGGGATTTCTCGAGTCT
CCCTACTGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_152846
Insert Size 1341 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_152846.1, NP_690059.1
RefSeq Size 1341 bp
RefSeq ORF 1341 bp
Locus ID 260320
UniProt ID Q8K3U6
Gene Summary Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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