Rb1cc1 (NM_001107901) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210423 representing NM_001107901 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTATATGTGTTTCTGGTTAACACTGGAACCACTCTGACATTTGACACTGAGCTAACTGTGCAAA CTGTGGCTGATCTGAAGCATGCCATTCAAAGCAAATACAAGATTGCTGTTCAGCACCAGGTGCTAGTTGT CAATGGAGGAGAATGCATGACTGCAGATCGAAGAGTGTGCACTTACAGCGCTGGGACGGACACAAATCCA ATTTTTCTTTTTAATAAAGAAATGATCTTATGTGACCGTGCACCTGCTATTCCTAAAACCACCTTTTCAA CAGAAAATGACATGGAAATAAAAGTCGAAGAGTCTCTTATGATGCCTGCAGTTTTTCACACTGTCGCTTC AAGGACACAGCTTGCAGTGGAAATGTATGAAGTTGCCAAGAAGCTTTGCTCTTTCTGTGAAGGCCTTGTC CATGATGAACATCTTCAGCACCAAGGCTGGGCTGCAATCATGGCCAATCTGGAGGACTGTTCAACTTCAT ACCAAAAACTCCTTTTCAAGTTTGAAAGTATTTATTCTGATTATCTGCAATCCATAGAAGACATCAAGTT AAAACTTACTCATTTAGGAACTGCGGTTTCAGTAATGGCCAAGATTCCACTGTTGGAGTGCCTAACCAGA CATAGTTACAGGGAATGTTTGGGAAGACCGGATTCTTTAAATGAACATGAAGGCTCAGAGAAAGCTGATA TGAAAAGATCTACTGAACTGGTGCTCTCTCCTGATATGCCTAGAACAACTAACACATCCTTGTTAACCTC ATTTCACAAGTCAGTGGAGCATGTAGCTCCAGATGTCACTGATGCTGAACAAGGCAAAGAACTTCGAGAA TCTTGTCAAAGTACTGTCCAGCAAGAAGACGCTCCAGTAGATGCTAAAGACAGTGATCTGCCTTTTTTTA ATGTTTCTTTGTTAGACTGGATAAATGTTCAAGATAGACCTAATGATGTGGAATCTCTGGTCAGGAAGTG CTTTGATTCTATGAGCAGGCTTGACCCAAAGATTATTCAACCGTTTATGTTAGAATGCCATCAAACTATT GCCAAACTTGATAATCAGAATATGAAAGCCATTAAAGGACTTGAAGATCGGCTGTATGCCTTGGACCAGA TGATTGCTAGCTGTAGCAGGCTGGTGAATGAACAGAAAGAGCTTGCTCAGGGATTTTTAGCTAATCAGAT GAGAGCAGAAAACTTGAAGGATGCATCTGTGCTACCTGATCTGTGCCTGAGTCATGCAAATCAGCTAATG ATTATGTTGCAAAACCACAGAAAACTGTTGGACATTAAACAGAAGTGTACCACTGCCAAACAAGAGCTAG CAAACAATCTGCATGTCAGACTGAAGTGGTGTTGTTTTGTGATGCTTCATGCTGATCAAGATGGAGAAAA ACTGCAAGCACTACTCCGCCTTGTAATAGAGCTATTAGAAAGAGTCAGAATTGTTGAGGCTCTTAGTACA GTTCCTCAGATGTACTGCCTAGCTGTTGTTGAGGTTGTAAGGAGAAAAATGTTCATTAAACACTACAGAG AGTGGGCCGGTGCTTTAGTCAAAGATGGAAAACAATTGTATGAAGCTGAAAAGTCAAAAAGGGAGTCCTT TGGGAAATTATTTAGGAAGTCCTTTTTAAGAAATCGTCTGTTTAAGGGACTGGACTCCTGGCCTTCCTCA TTTTGTACTCAGAAGCCTCGAAAATTTGACTGTGAACTTCCAGATATATCATTAAAAGATTTACAGTTTC TTCAATCATTTTGTCCTTCAGAAGTGCAGCCATTCCTCAGGGTTCCTTTACTTTGTGACTTTGAACCTCT ACACCAGCATGTACTTGCCCTACATAATTTGGTAAAAGCAGCACAAAGTTTGGATGAAATGTCACAGACC ATTACAGATCTGCTGAATGAACAAAAGGTATCCACAAGTCAAGCATCTCCACAGTCAGCTGCTTCTCCAA GAATAGAAAGTACAACAGGCATTACAACTACTACCTCACCAAAAGCTCCTCCTCCACTAACTGTTCAGGA TACTCTATATCCGGCAGCGTGTCCCTTAGAAGAGCTGTCTCCGGATAGCATCGATGCTCACACATTTGAT TTTGAAACCGTCTCCCATCCAAACACAGAGCAAACAGTTCACCAGGCTTCTATAGACTTGGACTCATTAG CAGAAAGCCCTGAGTCTGATTTTATGTCTGCTGTGAATGAGTTTGTGATAGAAGAAAATTTATCATCTCC GAACCCTATAAGTGATCCACAAAGTCCAGAAATGATGGTGGAGTCGCTTTACTCTTCAGTCATCAATGCA ATAGATAGTAGGCGCATGCAGGACACAAGCACACGTGGAAACGAGGGCTTTGGGGATCGCGCTGCGCTAC ATGTCCAGCTCGAGAAATGCAGAGTTGCTGCCCAAGACTCTCACATCAGTATACAAACCATCAAGGATGA TCTGTGCCACTTCAGAACATTTGTACAGAAAGAACAGTGTGACTTATCAAATTATTTAAAATGTACAGCT GTAGAAATAAGAAATATTATTGACAAAGTAAAATGTTCCCTAGAAATAACACTAAAGGAAAAACATCAGC AAGAACTCCAATCTTTAAAAATTGAGTATGAATGTAAACTTAATACTCTAGAAAAAGACAGTGAAGAAAA TGTAAATAAAATTTTAAAATTGAAAGAAGATTTAGTATCCCTTGAGGAGGCTTTACAAAATAAAGACAGT GAATTTGCGATAATTAAGCATGAAAAGGATGCTATTGTCTGCATGCAGCATGAGAAGGATCAGAAGTTGT TAGAGATGGAAAATATAATGCATACTCAAAACAGTGAAATTAAAGAACTGAAGCAGTCACGAGAAATGGT GTTAGAAGACCTGAAAAAACTGCATGATGAAAAAATAGAGTCATTGAGAGCTGAATTTCAGTGCCTAGAA CAAAATCACTTGAAGGAATTAGAAGACACACTGCACATCAGGCACACAGAGGAGTTTGAGAAAGTTATAA CAGACCACAAAGTATCTTTGGAGAAATTAAAAAAGGAAAATCAGCAAAAAATTGACCAGATGCTAGAATC TCATGCCTCAGCTATTCAGGAGAAAGAGCAGCAGCTGCAGGAATTGAAAGTCAAGGTTTCTGACTTGTCA GACATGAGGTGTAAGTTAGAGGTTGAACTTGCACTGAAGGAAGCAGAAACAGATGAAATAAAGATCTTGC TGGAAGAGAGCAGAACACAGCAGAAGGAAACACTGAAGTCTCTACTTGAACAAGAAACCGAAAACTTAAG AACAGAAATAAATAAATTAAACCAAAAAATTCATGATAATAGTGAGAGTTATCAGGTGGGTTTATCAGAG TTAAGATCTTTAATGACAATTGAAAAAGATCAGTGCATTTCAGAGTTAATCAGTAGACATGAGGAAGAAT CTAATATACTTAAGGCTGAATTAGACAGTGTTACAGCTTTGCATCACCAAGCATTTGAAATAGAAAAAAA ACTGAAAGAACAAATAGTTGAATTGCAGACTAAATTGAACTCAGAACTGAGTGCTCTTGAAAAACAGAAA GATGAAAAAATAACCCAACAAGAAGAGAAGTATAAAGCTCTTATACAGAACCTTGAGAAAGACAAGCAAA GATTGGCCATGAACCACGAACAAGACAAAGAACAGTTAATTCAGAAGCTTAATTTTGAAAAAGATGAAGC CATTCAAACTGCACTAGATGAATTTAAGCTGGAGAGAGAACTTGTTGAGAAAGAGTTATTAGAAAAAGTT AAACATCTTGAGAATCAAATAGCTAAAAGTCCCGCCTTTGAATCAGCCAGAGAAGATTCTTCAAGCTTAG TTGCTGAACTTCAAGAGAAACTTCAGGAAGAAAAAGCTAAGTTTCTAGAACAACTTGAAGAACAGGAGAA GAGAAAGAATGAGGAAATGCAGAATGTGCGCACCTCTCTGATTGCTGAACAGCAGACCAATTTTAACACA GTCTTAACAAGAGAGAAAATGAGAAAAGAAAACATAATAAATGATCTTAGCGATAAGCTAAAAAGTACAA TGCAGCAGCAAGAGCGGGATAAAGATTTGATAGAGTCGCTGTCTGAGGACCGAGCTCGTTTGCTTGAAGA GAAGAAGAAGCTTGAAGAGGAAGTGAGTAAACTGCGCACCAGCAGCGTCCTTCCCTCAGCACCCGTGCTT GCAGCCCCAGAGCTTTATGGCGCTTGTGCACCTGAGCTCCCAGGTGAGCCAGAGCGATCAGCCATGGAGA CGACGGACGAGGGAAGAGTGGATTCAGCAATGGAGACTAGCATGATGTCTGTCCAAGAAAATATGCTATC TGAAGAGAAGCAGAGGATCATGCTCCTAGAACGGACATTGCAATTGAAAGAAGAAGAAAACAAGAGGTTA AATCAAAGATTGATGTCTCAGAGCTTGTCCTCAGTATCTTCAAGGCATTCTGAAAAAATAGCCATTAGAG ATTTTCAGGTGGGAGATTTGGTTCTCATCATCCTAGATGAGCGGCATGACAATTATGTGTTGTTTACTGT TAGTCCTACTTTGTATTTTCTGCATTCAGAGTCTCTTCCTGCCCTGGATCTCAAACCAGGTGAGGGAGCT TCAGGTGTATCTAGAAGACCCTGGGTCCTTGGAAAAGTAATGGAAAAGGAATACTGTCAAGCCAAAAAGG CACAAAACAGATTTAAAGTTCCTTTGGGGACAAAGTTTTACAGAGTGAAAGCTGTGTCATGGAATAAGAA AGTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001107901
Insert Size	4767 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001107901.1, NP_001101371.1
RefSeq Size	7197 bp
RefSeq ORF	4767 bp
Locus ID	312927

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