Pah (NM_012619) Rat Untagged Clone

CAT#: RN210811

Pah (untagged ORF) - Rat phenylalanine hydroxylase (Pah), (10 ug)



  "NM_012619" in other vectors (3)

CNY 5,800.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN210811 representing NM_012619
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCTGTTGTCCTGGAGAATGGAGTCCTGAGCAGAAAACTCTCAGACTTTGGGCAGGAAACAAGCT
ATATTGAAGACAACTCCAATCAAAATGGTGCCATATCTCTGATATTCTCACTCAAAGAGGAAGTTGGTGC
CCTAGCCAAGGTCCTGCGCTTATTTGAGGAGAACGATATCAACCTGACACACATTGAATCCAGACCTTCT
CGTTTAAACAAAGATGAGTATGAGTTCTTCACTTATCTGGATAAGCGTAGCAAACCTGTTCTGGGCAGCA
TCATCAAGAGCCTGAGGAATGACATTGGTGCCACTGTCCACGAGCTTTCCCGAGACAAGGAAAAGAACAC
AGTGCCCTGGTTCCCAAGGACCATTCAGGAGTTGGACCGATTCGCCAATCAGATTCTCAGCTATGGAGCC
GAACTGGATGCGGACCACCCAGGCTTTAAAGATCCTGTGTACCGGGCGAGACGAAAGCAGTTTGCTGACA
TTGCCTACAACTACCGCCATGGCCAGCCCATCCCTCGGGTGGAATACACAGAGGAAGAGAAGCAGACCTG
GGGAACGGTGTTCAGGACCTTGAAGGCCTTGTATAAAACACATGCCTGCTACGAGCACAACCACATTTTC
CCACTTCTGGAAAAGTACTGCGGTTTCCGTGAAGACAACATTCCACAGCTGGAAGATGTTTCTCAATTTC
TGCAGACTTGTACTGGTTTCCGCCTCCGACCTGTTGCTGGCTTACTGTCATCTCGAGATTTCTTGGGTGG
CCTGGCCTTCCGAGTCTTCCACTGCACACAGTACATTAGGCATGGATCGAAGCCCATGTACACACCTGAA
CCTGACATCTGCCATGAACTCTTGGGACATGTGCCTTTGTTTTCAGATCGCAGCTTTGCCCAGTTTTCTC
AGGAAATTGGGCTTGCATCACTGGGGGCACCTGATGAGTACATTGAGAAACTGGCCACAATTTACTGGTT
TACTGTGGAGTTTGGGCTTTGCAAGGAAGGAGATTCTATAAAGGCATATGGTGCTGGGCTCTTGTCATCC
TTTGGAGAATTACAGTACTGTTTATCAGACAAGCCAAAGCTCCTCCCCCTGGAGCTAGAGAAGATAGCCT
GCCAGGAGTATTCTGTCACAGAGTTCCAGCCCTTGTACTACGTGGCGGAGAGTTTCAGTGATGCCAAGGA
GAAAGTGAGGACTTTTGCTGCCACGATCCCTCGGCCCTTCTCGGTTCGCTATGACCCTTACACTCAAAGG
GTTGAGGTCCTGGACAATACTCAGCAGTTGAAGATTTTAGCTGACTCCATTAATAGTGAGGTCGGAATCC
TTTGCAATGCCCTGCAGAAGATAAAGTCGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_012619
Insert Size 1362 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_012619.2, NP_036751.2
RefSeq Size 2040 bp
RefSeq ORF 1362 bp
Locus ID 24616
Gene Summary catalyzes the conversion of phenylalanine to tyrosine; deficiency in humans causes genetic disorder phenylketonuria [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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