Sos1 (NM_001100716) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN210901 representing NM_001100716 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGCGCAGCAGCTGCCCTACGAGTTTTTCAGCGAGGAGAACGCGCCCAAGTGGCGGGGGCTGCTGG TGCCTGCGCTGAAAAAGGTTCAGGGGCAAGTTCACCCTACTCTTGAGTCTAATGAGGATGCTCTTCAGTA TGTTGAAGAATTAATTTTGCAATTACTAAATATGCTATGCCAAGCTCAGCCCCGGAGTGCTTCAGATGTG GAGGAACGTGTTCAAAAAAGTTTCCCTCATCCAATTGATAAGTGGGCAATAGCTGATGCCCAATCAGCTA TTGAAAAGAGGAAGAGAAGGAACCCTTTATCCCTGCCAGCAGAAAGAATTCATCATTTATTAAGGGAGGT CCTGGGTTATAAAATTGACCACCAGGTTTCTGTTTACATAGTAGCAGTATTAGAATACATTTCTGCAGAT ATTTTAAAGCTGGTGGGGAATTATGTAAGAAATATACGGCATTATGAAATTACAAAACAAGACATTAAAG TGGCAATGTGTGCTGATAAGGTATTGATGGATATGTTTCATCAAGATGTAGAAGATATAAATATCTTATC TTTAACTGATGAAGAACCTTCCACCTCAGGAGAACAAACTTACTATGATTTGGTAAAAGCATTCATGGCA GAAATTCGACAATATATAAGAGAATTAAATCTAATTATAAAGGTTTTTCGAGAACCCTTTGTCTCTAATT CCAAGTTGTTTTCATCTTATGATGTAGAAAACATATTTAGTCGTATAGTGGATATCCATGAACTTAGTGT AAAGTTACTGGGCCATATAGAAGACACTGTAGAGATGACAGATGAAGGCAGTCCCCACCCGTTAGTCGGA AGCTGTTTTGAAGACTTAGCAGAAGAACTGGCCTTTGACCCATATGAGTCCTATGCTCGGGATGTTCTGC GGCCCGGATTCCATGAGCGTTTTCTCAGTCAGCTATCAAAGCCTGGGGCAGCACTTTATCTGCAGTCCAT AGGTGAAGGCTTCAAAGAGGCCGTTCAGTATGTCCTGCCCCGGCTGCTGCTCGCCCCTGTGTACCACTGT CTGCATTACTTTGAACTTCTGAAGCAGTTAGAAGAAAAGAGCGAAGATCAAGAAGATAAGGAGTGTATGA AACAAGCAATAACAGCCCTGCTTAATGTCCAAAGTGGCATGGAAAAAATTTGCTCCAAAAATCTTGCAAA ACGAAGACTGAGTGAGTCTGCATGTCGGTTTTACAGCCAGCAAATGAAGGGGAAACAACTGGCCATCAAG AAGATGAATGAGATTCAGAAGAACATTGATGGTTGGGAGGGGAAAGACATTGGACAATGCTGCAATGAGT TTATAATGGAAGGAACTCTTACACGTGTAGGAGCCAAACACGAGAGACACATATTTCTCTTCGATGGCTT AATGATTTGCTGTAAATCAAATCATGGGCAGCCAAGACTTCCTGGTGCTAGCAATGCAGAATATCGGCTT AAAGAAAAGTTTTTTATGCGAAAGGTACAGATTAACGATAAGGATGACACCAGTGAATACAAGCACGCTT TCGAAATAATTCTGAAGGATGGAAATAGTGTTATATTTTCTGCCAAGTCAGCTGAAGAGAAGAATAACTG GATGGCAGCCCTGATATCCCTGCAGTACCGCAGCACGCTGGAGAGAATGCTGGACGTTACAATGCTGCAG GAGGAGAAGGAGGAGCACATGAGGCTGCCGAGTGCTGACGTGTACAGGTTTGCAGAGCCCGACTCTGAGG AGAACATTCTGTTTGAAGAGAACGTGCAGCCCAAGGCTGGCATCCCCATCATCAAGGCAGGGACAGTGGT TAAGCTGATTGAGAGGCTCACGTACCACATGTACGCAGATCCAAATTTTGTTCGGACATTTCTTACAACA TACAGATCATTTTGCAAACCTCAAGAACTACTGAATCTTCTAATAGAAAGATTTGAAATTCCAGAGCCTG AGCCAACAGAAGCTGATCGCATAGCTATAGAGAATGGAGATCAGCCCCTGAGCGCAGAGCTGAAAAGGTT TAGAAAGGAGTATATCCAGCCTGTACAGCTGAGGGTGTTAAACGTGTGTCGGCACTGGGTGGAGCACCAT TTCTATGACTTTGAAAGAGATGTAGACCTTTTACAGAGAATGGAGGAATTTATTGGAACAGTAAGAGGTA AAGCAATGAAAAAATGGGTCGAATCCATCACTAAGATAATCCAAAGGAAAAAAATTGCAAGAGACAATGG CCCAGGTCATAATATTACATTTCAGAATTCACCTCCCACAGTTGAGTGGCACATAAGCAGACCTGGGCAT ATAGAGACTTTTGACTTGCTCACCCTACACCCAATAGAAATTGCTCGACAACTCACTTTACTTGAATCAG ATCTATACCGAGCTGTGCAGCCATCAGAATTAGTTGGAAGTGTGTGGACAAAAGAAGATAAAGAAATTAA CTCTCCTAACCTTCTGAAAATGATTCGGCACACCACCAACCTCACTCTGTGGTTTGAGAAATGTATTGTA GAAACAGAGAACTTAGAAGAAAGAGTAGCAGTAGTGAGTCGAATAATTGAGATTCTACAAGTCTTTCAAG AGCTGAACAACTTCAATGGTGTCCTGGAAGTTGTTAGTGCTATGAACTCGTCACCTGTTTACAGACTGGA CCACACATTTGAGCAAATACCAAGTAGACAAAAGAAAATTTTAGAAGAAGCTCATGAGTTGAGTGAAGAT CACTATAAGAAATATTTGGCAAAACTCAGGTCTATTAATCCACCATGTGTGCCTTTCTTTGGAATTTATC TCACAAATATCTTGAAAACAGAAGAAGGCAACCCTGAGGTCCTAAGAAGACATGGAAAAGAGCTTATCAA CTTCAGCAAGAGGAGGAGAGTGGCCGAGATAACAGGAGAGATCCAGCAGTACCAAAACCAGCCTTACTGC TTACGGGTAGAGTCAGACATCAAGAGATTCTTTGAAAACTTGAATCCAATGGGAAATAGCATGGAGAAGG AATTTACAGACTATCTATTCAACAAATCCCTAGAAATAGAACCACGGAACCCTAAGCCTCTTCCAAGATT TCCAAAAAAATACAGCTATCCCCTAAAATCTCCTGGTGTTCGTCCATCAAATCCAAGACCAGGAACCATG AGGCACCCTACACCTCTGCAGCAGGAGCCAAGGAAAATCAGCTACAGTCGGATTCCTGAGAGCGAGACAG AAAGTACAGCGTCGGCACCAAACTCCCCGAGAACACCGCTGACGCCGCCTCCTGCGTCCAGCGCCTCCAG TAACACAGACGTCTGCAGCGTGTTCGATTCCGACCACTCCGCAAGCCCTTTTCACTCAAGATCTGCTTCA GTCTCATCTATAAGTTTATCCAAGGGCACCGAGGAAGTGCCTGTCCCTCCTCCTGTCCCCCCTCGAAGAC GACCAGAGTCTGCCCCAGCGGAATCCTCCCCATCCAAGATTATGTCTAAGCACTTGGATAGCCCCCCAGC GATTCCTCCTAGGCAGCCCACATCGAAAGCCTATTCACCACGATACTCAATATCAGATCGGACCTCTATA TCAGATCCTCCCGAGAGCCCTCCCTTGTTACCACCACGAGAACCTGTGAGGACACCCGATGTTTTCTCAA GCTCGCCATTACATCTCCAACCTCCCCCGTTGGGCAAAAAGAGTGACCATGGCAATGCCTTCTTCCCAAA CAGCCCATCCCCCTTTACACCGCCACCTCCTCAAACCCCGTCTCCTCACGGCACGAGAAGGCATCTGCCA TCACCACCACTGACACAGGAAGTGGACCTCCATTCCATTGCTGGGCCTCCCGTTCCTCCACGGCAAAGCA CTTCTCAACTTATCCCCAAACTCCCTCCAAAAACTTACAAAAGGGAGCACACACACCCATCCATGCACAG AGATGGACCGCCACTGCTGGAGAATGCCCATTCTTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100716
Insert Size	3960 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100716.1, NP_001094186.1
RefSeq Size	6018 bp
RefSeq ORF	3960 bp
Locus ID	313845

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