Plec (NM_022401) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Pcn; Plec1; Pltn
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211158 representing NM_022401 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGCTGGCATGCTCATGCCACTGGACCAGCTTCGGGCCATCTATGAGGTGCTCTTTCGTGAGGGGG TGATGGTTGCCAAGAAGGACCGGCGACCCCGAAGCCTGCATCCCCATGTGCCCGGCGTCACCAATCTACA GGTCATGCGTGCCATGACCTCGCTGAAAGCTCGGGGCCTGGTGCGGGAGACCTTTGCCTGGTGCCACTTC TACTGGTACCTGACCAACGAGGGCATCGACCACCTACGCCAGTACCTACACCTGCCACCGGAGATCGTAC CTGCCTCTCTGCAGCGTGTGCGCCGCCCTGTTGCCATGGTGATGCCTGCACGTCGTCGCTCCCCCCATGT GCAGACCATGCAAGGTCCCTTAGGCTGTCCACCAAAGAGGGGCCCTCTGCCAGCTGAGGACCCTGCCCGG GAGGAGCGGCAGGTCTATCGCAGGAAGGAGCGTGAGGAAGGGGCACCTGAAACCCCTGTGGTGTCTGCCA CCATCGTGGGGACCCTGGCCAGGCCAGGCCCAGAGCCCACCCCAGCCACAGATGAACGAGACCGTGTGCA GAAGAAAACTTTCACCAAGTGGGTCAATAAACACCTTATCAAGGCTCAAAGGCACATCAGTGACCTGTAT GAAGACCTCCGTGATGGCCACAACCTCATCTCCCTGCTGGAAGTCCTCTCAGGAGACAGCCTGCCCCGAG AGAAGGGGAGGATGCGTTTCCACAAGCTGCAAAACGTTCAGATTGCCCTGGACTATCTCCGACATCGGCA GGTGAAGTTGGTGAACATCAGAAATGATGACATCGCCGATGGCAACCCCAAGCTGACCCTGGGCCTGATC TGGACAATCATCCTGCACTTCCAGATCTCAGACATTCAGGTGAGCGGCCAGTCCGAGGACATGACAGCCA AGGAGAAGCTGCTGCTGTGGTCTCAGCGTATGGTAGAGGGCTACCAAGGCCTGCGCTGTGACAACTTCAC CACCAGCTGGCGCGACGGCCGGCTCTTCAATGCTATCATCCACAGGCACAAGCCCATGCTCATAGACATG AATAAAGTGTACCGACAGACCAACCTGGAGAACCTAGACCAGGCCTTCTCCGTGGCAGAGCGGGACCTGG GAGTTACCAGGCTCCTGGACCCAGAAGATGTGGATGTCCCTCAGCCTGATGAGAAGTCCATCATCACCTA CGTTTCATCCCTGTATGATGCCATGCCCCGTGTGCCGGGCGCACAGGATGGAGTGAGGGCCAATGAGCTG CAGCTTCGTTGGCAAGAGTACCGGGAGCTTGTGCTGCTGCTGCTACAGTGGATCCGGCACCACACCGCTG CTTTTGAGGAGCGCAAGTTCCCCTCCAGCTTTGAGGAGATTGAGATCCTATGGTGCCAGTTTCTGAAGTT CAAGGAGACAGAACTTCCTGCCAAGGAGGCAGACAAGAACCGGTCCAAAGGCATCTACCAGTCTTTGGAG GGAGCAGTACAAGCAGGCCAGCTCAAGATTCCCCCTGGCTACCACCCGCTAGACGTGGAAAAGGAGTGGG GCAAGCTGCACGTGGCCATCCTGGAGCGGGAGAAGCAACTCCGGAGCGAGTTTGAGAGGCTGGAGTGTCT TCAGCGCATTGTGAGCAAGCTACAGATGGAGGCTGGGCTATGTGAGGAGCAGCTGAACCAGGCCGATTCC CTACTGCAGTCGGATATTCGGCTGCTGGCCTCAGGCAAGGCGGCACAGCGGGCTGGGGAAGTGGAGAGAG ACCTGGACAAGGCTGATGGTATGATCCGGCTGTTGTTCAATGATGTGCAGACCCTTAAAGATGGGCGGCA TCCACAGGGTGAACAGATGTACCGGAGGGTGTATCGTCTGCATGAGCGCCTGGTAGCCATCCGCACTGAA TACAACCTCCGGCTGAAGGCAGGGGTGGGTGCCCCTGTGACCCAGGTGACCCTGCAGAGCACACAGAGGC GCCCAGAGCTAGAGGACTCCACACTGCGTTACCTGCACGACCTGCTGGCATGGGTGGAGGAGAACCAGCG TCGAATAGACGGTGCTGAGTGGGGCGTGGACTTGCCCAGTGTAGAGGCACAGCTGGGCAGCCACCGAGGC ATGCATCAGTCTATTGAGGAATTCCGGGCCAAGATCGAGCGGGCTCGGAATGATGAGAGCCAGCTCTCCC CTGCCACCCGAGGTGCCTACCGAGACTGCCTGGGCCGCCTGGACCTGCAGTATGCAAAGCTGCTGAACTC CTCCAAGGCCCGCCTCCGGTCCCTGGAGAGCTTGCATGGGTTTGTGGCGGCAGCTACCAAGGAGCTGATG TGGCTGAATGAGAAGGAAGAGGAAGAAGTGGGCTTTGATTGGAGTGACCGCAACACCAACATGGCTGCCA AGAAAGAAAGTTACTCGGCCCTGATGCGTGAGCTGGAGATGAAGGAAAAGAAAATTAAGGAGATCCAGAA CACGGGGGACAGGTTGCTGCGGGAAGACCATCCTGCCCGGCCCACAGTGGAGTCCTTCCAGGCTGCCCTG CAGACACAGTGGAGCTGGATGCTGCAGCTGTGTTGCTGCATTGAAGCGCACTTGAAAGAGAACACAGCCT ACTTCCAGTTCTTCTCAGATGTTCGGGAGGCTGAGGAACAGTTGCAGAAACTACAGGAGACGTTACGCAG GAAGTACAGCTGTGACCGCTCCATCACTGTCACAAGGCTTGAGGACCTGCTGCAGGATGCCCAGGATGAG AAGGAGCAACTGAATGAGTACAAAGGGCACCTCTCAGGCCTGGCCAAGCGGGCCAAGGCTATTGTGCAGC TGAAGCCACGCAACCCTGCCCACCCTGTGCGGGGTCACGTGCCCCTGCTAGCTGTGTGTGACTACAAGCA GGTGGAGGTGACTGTGCACAAGGGTGACCAATGCCAGCTGGTGGGCCCTGCCCAGCCGTTCCACTGGAAG GTGCTCGGTGGTTCCAGCAGTGAGGCTGCCGTGCCTTCTGTGTGCTTTCTTGTGCCGCCACCCAACCAGG AGGCCCAGGAAGCTGTTGCTAGGCTGGAGGCCCAGCATCAGGCCCTGGTTACTCTGTGGCACCAGCTTCA CGTGGACATGAAGAGTCTTCTGGCATGGCAGAGCCTCAATCGTGACATACAGCTCATCCGGTCCTGGTCC CTAGTCACGTTCCGCACGCTGAAGCCCGAGGAGCAGCGGCAAGCTCTGCGCAACCTGGAGTTGCACTACC AGGCCTTCCTTCGAGACAGCCAGGACGCTGGTGGCTTTGGGCCCGAGGACCGGCTGGTGGCAGAGCGCGA ATATGGATCTTGTAGTCGCCACTACCAGCAGCTGCTACAAAGCCTGGAGCAGGGTGAGCAGGAGGAGTCT CGCTGTCAGCGATGCATCTCGGAGCTCAAGGACATTCGGCTGCAACTGGAGGCCTGTGAGACTCGGACTG TGCACCGTCTGCGGCTGCCACTGGATAAAGACCCCGCACGGGAGTGTGCCCAGCGCATCGCTGAGCAACA GAAAGCACAGGCTGAGGTGGAGGGGCTGGGCAAGGGAGTTGCCCGGCTGTCTGCTGAGGCTGAGAAAGTT CTGGCCTTGCCAGAGCCGTCACCTGCTGCACCAACTCTGCGCTCGGAGTTGGAATTGACCCTGGGCAAGC TGGAACAGGTCAGAAGCCTGTCTGCCATCTACTTGGAGAAACTCAAGACCATCAGCTTGGTAATTCGCAG TACCCAGGGGGCTGAGGAGGTGCTTAAAACACACGAGGAGCACCTGAAGGAGGCCCAGGCCGTGCCTGCC ACACTCCAAGAGCTCGAAGTCACCAAGGCTTCACTAAAGAAGCTGCGGGCCCAGGCGGAGGCACAGCAGC CTGTATTCAACACCCTACGAGATGAGCTGAGGGGGGCACAGGAAGTTGGTGAACGGCTACAGCAGCGGCA TGGTGAGCGGGACGTGGAAGTAGAGCGCTGGCGAGAACGTGTCACTCAGTTGCTGGAGCGCTGGCAGGCT GTGCTAGCCCAGACTGATGTGCGGCAGCGGGAGCTTGAACAGCTGGGCCGCCAACTTCGCTACTACCGTG AAAGTGCGGATCCGCTGAGCTCCTGGCTGCAGGATGCCAAGAGCCGGCAAGAGCAGATCCAGGCTGTGCC AATAGCCAACAGTCAGGCTGCACGAGAACAGCTGCGCCAGGAGAAGGCCCTGCTGGAGGAGATTGAGCGC CATGGTGAGAAGGTTGAGGAGTGCCAGAAGTTTGCTAAGCAGTACATCAATGCAATCAAGGACTATGAGC TCCAGCTGATCACCTACAAGGCTCAGCTTGAACCTGTGGCCTCCCCCGCCAAGAAGCCCAAGGTTCAGTC TGGATCGGAGAGCGTCATCCAGGAGTACGTGGATCTGCGTACACGCTACAGTGAGCTGACCACACTCACG AGTCAGTACATCAAGTTCATCAGTGAGACACTGCGCCGCATGGAAGAGGAAGAGCGGCTGGCTGAGCAAC AGCGGGCAGAGGAGCGGGAGCGCCTGGCCGAGGTGGAGGCCGCGCTGGAGAAGCAGCGGCAGCTGGCTGA GGCCCATGCCCAGGCCAAGGCACAGGCCGAGCTGGAGGCACGAGAACTGCAGCGGCGCATGCAGGAGGAG GTGACGCGGCGCGAGGAGGCGGCGGTGGACGCACAGCAACAGAAGCGCAGCATCCAAGAGGAGCTGCAGC ATCTGCGGCAAAGCTCAGAGGCAGAGATCCAGGCCAAGGCCCAGCAGGTGGAGGCTGCAGAGCGCAGCCG CATGCGCATTGAGGAAGAGATCCGCGTAGTCCGTCTGCAGCTAGAGACAACTGAGCGTCAGCGTGGAGGG GCGGAGGATGAGCTGCAGGCTCTGCGTGCACGGGCTGAGGAGGCAGAAGCACAGAAGCGGCAGGCTCAGG AGGAAGCCGAGCGCTTGCGGAGGCAGGTGCAGGATGAGAGCCAACGCAAACGGCAGGCGGAGGCCGAGCT GGCCCTGCGTGTGAAGGCAGAAGCGGAGGCAGCGCGAGAGAAGCAGCGGGCCCTGCAGGCTCTGGATGAA CTGAAACTGCAGGCCGAGGAGGCCGAACGGCGGCTGCGCCAAGCCGAGGCAGAGAGGGCTCGCCAAGTGC AGGTAGCCCTGGAGACAGCGCAGCGTAGTGCAGAAGTGGAGCTGCAGAGCAAGCGTGCGTCCTTTGCAGA GAAGACCGCACAGTTGGAGCGCACGCTGCAGGAAGAGCACGTGACAGTGACACAGCTGCGGGAGGAGGCG GAACGGCGGGCACAGCAGCAGGCTGAAGCCGAGCGAGCCCGTGAGGAAGCCGAGCGGGAGCTGGAGCGCT GGCAGCTGAAGGCCAATGAGGCGCTGCGGCTGCGGCTGCAGGCAGAGGAGGTGGCACAGCAGAAGAGCCT GGCCCAGGCCGATGCAGAGAAGCAGAAGGAAGAGGCAGAACGGGAAGCCCGGCGGCGGGGCAAGGCAGAG GAGCAGGCCGTGCGGCAGCGAGAGCTGGCTGAGCAGGAGCTGGAGAAGCAGCGGCAGCTGGCAGAGGGCA CCGCCCAGCAGCGCCTGGCTGCCGAGCAGGAGCTGATTCGCCTGCGGGCAGAGACGGAGCAAGGTGAGCA GCAGCGGCAGCTGCTGGAGGAAGAGCTGGCCCGGCTACAGCACGAAGCGACAGCAGCCACACAGAAGCGC CAGGAGCTGGAGGCTGAGCTGGCGAAGGTTCGGGCAGAGATGGAGGTACTGCTGGCCAGCAAGGCACGAG CCGAAGAGGAGTCTCGCTCCACCAGTGAAAAGTCCAAGCAGAGGCTGGAAGCTGAGGCAGGGCGGTTTCG AGAGCTGGCTGAGGAGGCTGCCCGCCTGCGTGCTCTGGCCGAGGAGGCCAAGCGGCAGCGGCAGTTGGCC GAGGAGGACGCGGCACGCCAGCGGGCCGAGGCGGAGCGAGTGCTTACGGAGAAGCTGGCTGCCATCAGTG AGGCCACAAGGCTCAAGACGGAGGCAGAGATTGCACTCAAAGAGAAGGAGGCCGAGAACGAGCGCCTGAG GCGCCTGGCTGAAGATGAGGCCTTCCAGCGGCGCCGGCTGGAGGAGCAGGCAGCACAGCACAAGGCAGAC ATAGAGGAGCGCCTGGCCCAGCTGCGCAAGGCATCCGAGAGCGAGCTGGAGCGACAGAAGGGGTTGGTGG AGGATACCCTGCGGCAGCGGCGGCAGGTGGAGGAGGAGATCATGGCTCTGAAGGCGAGCTTCGAGAAGGC CGCGGCTGGCAAGGCAGAACTGGAGCTGGAGCTTGGCCGCATCCGCAGCAATGCCGAGGACACCATGCGC AGCAAGGAGCTGGCCGAGCAGGAGGCAGCGCGGCAGCGGCAGTTGGCAGCTGAGGAGGAGCAGAGGCGCC GGGAAGCCGAGGAGCGGGTGCAGAGGAGCCTGGCAGCGGAGGAGGAAGCCGCACGGCAGCGCAAGGTCGC ACTGGAGGAAGTCGAGCGGCTCAAGGCCAAGGTTGAGGAAGCGCGGCGCCTGCGAGAGCGAGCTGAGCAG GAGTCTGCGAGGCAGCTGCAGCTGGCCCAGGAGGCTGCCCAGAAACGGCTGCAGGCGGAGGAGAAGGCGC ACGCCTTTGTGGTGCAGCAGCGAGAAGAGGAGCTGCAGCAGACTCTTCAGCAAGAGCAGAACATGCTGGA GCGGCTGCGGAGCGAGGCAGAGGCAGCGCGGCGAGCTGCTGAGGAGGCGGAGGAGGCCCGGGAGCAGGCA GAACGTGAGGCAGCGCAGTCTAGGAAGCAAGTGGAAGAGGCCGAGCGGCTGAAGCAGTCGGCAGAGGAGC AGGCTCAGGCCCAGGCCCAGGCGCAGGCGGCTGCAGAGAAACTGCGCAAGGAAGCGGAGCAGGAGGCGGC GCGTCGGGCCCAGGCGGAGCAGGCTGCGTTGAAACAGAAGCAGGCAGCCGACGCGGAGATGGAGAAGCAC AAGAAGTTTGCAGAGCAGACGCTACGGCAGAAGGCTCAGGTAGAGCAGGAGCTGACCACGCTGAGGCTGC AGCTCGAGGAGACCGACCACCAGAAGAGCATCCTGGATGAGGAGCTGCAGCGGCTAAAGGCTGAGGTAAC AGAGGCAGCCCGGCAGCGTAGCCAGGTAGAGGAGGAGCTCTTCTCTGTCCGCGTGCAGATGGAGGAGCTG GGCAAACTCAAGGCTCGCATTGAAGCTGAAAACCGGGCACTCATCCTTCGTGACAAGGACAACACACAGC GCTTCCTGGAGGAGGAGGCCGAGAAGATGAAACAGGTGGCAGAGGAAGCTGCACGGTTGAGCGTAGCTGC CCAGGAGGCAGCAAGGCTGCGGCAGCTAGCCGAGGAGGACCTGGCCCAGCAGCGGGCCCTGGCGGAGAAG ATGCTGAAGGAGAAGATGCAGGCGGTGCAGGAAGCCACAAGGCTCAAGGCTGAGGCTGAGCTGCTGCAGC AGCAGAAGGAGCTGGCACAGGAGCAGGCCCGGCGGCTGCAGGAGGACAAGGAGCAAATGGCTCAGCAGTT GGTAGAGGAGACACAGGGTTTCCAGCGGACCCTGGAGGCTGAGCGGCAGCGGCAGCTAGAAATGAGCGCA GAGGCTGAACGCCTCAAGTTGCGCATGGCTGAGATGAGCCGGGCTCAGGCCCGTGCAGAGGAGGATGCCC AGCGCTTCCGGAAGCAGGCTGAAGAGATCGGCGAAAAGCTGCACCGCACTGAACTCGCTACACAGGAGAA GGTGACATTGGTGCAGACTCTCGAGATCCAGCGACAGCAGAGTGACCAAGATGCCGAGCGTCTGAGGGAG GCCATTGCTGAGCTGGAGCGTGAGAAGGAGAAGCTCAAGCAGGAGGCGAAGTTACTGCAGCTCAAGTCTG AGGAGATGCAGACTGTGCAGCAGGAGCAGATACTGCAGGAGACACAGGCCCTGCAGAAGAGCTTTCTCTC TGAGAAGGACAGCTTGCTGCAACGCGAACGCTTCATCGAGCAGGAGAAGGCCAAGCTGGAGCAGCTTTTC CAGGACGAGGTGGCAAAAGCAAAACAGCTGCAGGAGGAGCAGCAGCGGCAGCAGCAGCAGATGGAGCAGG AAAAGCAGGAGCTGGTGGCCAGCATGGAGGAGGCCCGGAGGCGGCAGCGTGAGGCAGAGGAGGGTGTGAG GCGCAAGCAAGAGGAACTGCAGCGTCTGGAGCAGCAGCGGCAGCAGCAGGAGAAACTACTGGCAGAGGAG AACCAGAGGCTGCGGGAGCGGCTGCAGCGCCTGGAGGAAGAGCACCGAGCTGCGTTGGCGCACTCTGAGG AGATCGCCACCTCCCAGGCTGCTGCCACAAAAGCACTGCCCAATGGCCGCGACGCACTTGATGGCCCCTC CATGGAGGCCGAGCCCGAGTACACCTTCGAGGGATTACGTCAGAAGGTGCCAGCTCAGCAGCTACAGGAA GCAGGCATTCTGAGCATGGAGGAACTGCAGCGTTTGACACAGGGTCACACCACGGTGGCTGAGCTCACGC AGCGGGAAGATGTGCACCGATACCTGAAGGGCGGCAGCAGCATCGCAGGATTGCTCCTGAAGCCCACCAA TGAGAAACTGAGTGTCTACACAGCCCTACAGCGGCAGCTGCTCAGCCCTGGAACAGCCCTTATCTTGCTT GAGGCCCAGGCAGCCTCGGGCTTCCTGCTGGACCCTGTGCGGAACCGGCGGCTGACGGTCAATGAGGCTG TGAAGGAGGGTGTGGTGGGTCCCGAGCTGCACCACAAGCTGCTGTCAGCTGAGCGTGCCGTCACTGGCTA CAAGGACCCTTACACAGGAGAACAGATCTCTCTCTTCCAGGCCATGAAGAAGGACCTCATTGTCAGGGAC CATGGCATCCGCCTGCTGGAAGCCCAGATCGCCACAGGTGGCATCATTGACCCTGTACACAGCCACCGTG TTCCCGTGGACGTGGCCTACCAGCGTGGCTACTTCGATGAGGAGATGAACCGTGTGCTGGCTGACCCAAG CGATGACACCAAGGGCTTCTTTGACCCCAACACTCACGAGAACCTCACGTACCTGCAGCTGCTGGAGCGC TGTGTGGAGGACCCCGAGACAGGCCTGCGCCTCCTGCCACTCACAGACAAGGCTGCCAAGGGTGGTGAGC TGGTGTACACTGACACGGAGGCCCGTGACGTCTTCGAAAAGGCCACAGTGTCTGCACCATTCGGCAAGTT CCAGGGCAAGACCGTGACCATCTGGGAGATCATCAACTCAGAGTACTTCACAGCGGAGCAGCGACGGGAC CTGCTCCGGCAGTTCCGCACGGGCCGCATCACGGTGGAGAAGATCATCAAGATTGTCATCACGGTGGTAG AGGAACACGAGCGGAAGGGCCAGCTCTGCTTTGAGGGCCTCCGTGCCCTTGTGCCTGCTGCAGAGCTGCT GGACAGTGGAGTCATCAGTCATGAAGTCTACCAGCAGCTGCAGCGGGGTGAGCGCTCTGTGCGGGAAGTG GCCGAGGCAGACGAGGTGAGGCAGGCCCTGCGGGGTACCAGTGTCATTGCCGGTGTGTGGCTGGAAGAAG CAGGGCAGAAGCTGAGCATCTATGAGGCCCTGAGGAGAGATTTGCTGCAGCCAGAGGTGGCTGTGGCCTT GCTGGAGGCCCAGGCTGGCACTGGGCACATCATTGACCCTGCCACGAGTGCCAGGCTGACTGTGGATGAG GCAGTGCGTGCTGGCCTGGTGGGTCCTGAGATGCACGAGAAGCTCTTGTCAGCTGAGAAGGCTGTAACAG GCTATAGGGATCCCTACTCGGGACAGAGCGTCTCGCTCTTCCAGGCTCTGAAGAAGGGTCTCATCCCCCG AGAACAGGGCCTGCGCCTGCTGGATGCCCAGTTATCCACTGGTGGCATTGTAGACCCCAGCAAAAGCCAC CGTGTGCCCCTGGATGTTGCCTATGCCCGGGGCTACCTGGACAAAGAGACTAACAGGGCCCTGACGTCAC CCAGAGACGATGCCAGAGTCTACCTTGACCCCAGCACCCGGGAGCCAGTCACCTACAGCCAGCTCCAACA GCGGTGCCGGTCTGACCAGCTGACTGGGTTGAGCCTACTGCCCCTCTCAGAGAAGGCCGTCCGGGCCCGG CAGGAAGAGGTCTACTCTGAGCTCCAGGCCCGTGAGACATTGGAGAAGGCCAAGGTGGAGGTTCCTGTGG GCGGCTTTAAGGGCAGGGCGCTGACAGTGTGGGAGCTCATAAGCTCGGAATACTTCACTGAGGAGCAGCG GCAGGAGCTGCTACGGCAGTTCCGCACAGGCAAGGTCACTGTAGAGAAGGTCATCAAGATTCTTATCACC ATTGTGGAGGAGGTGGAGACTCAACGGCAGGAGAGACTGTCCTTCAGTGGCCTCCGTGCCCCTGTGCCGG CCAGTGAGCTCCTGGCCTCCAAGATCCTCAGCAGAACTCAGTTTGAGCAGCTCAAGGATGGCAAGACATC AGTCAAAGATCTGTCAGAGGTGGGCTCTGTGCGGACACTGCTGCAAGGCAGCGGCTGCCTGGCTGGCATC TATCTGGAGGACTCGAAGGAGAAAGTAACCATCTATGAGGCCATGCGCCGGGGCCTCCTCAGACCCAGCA CAGCCACACTCCTGCTGGAGGCCCAGGCGGCCACTGGTTTTCTAGTGGACCCTGTGCGGAACCAACGTCT GTACGTCCATGAAGCTGTCAAGGCTGGAGTGGTGGGCCCGGAGCTCCATGAGAAGCTGCTGTCGGCTGAG AAGGCGGTCACTGGTTACAAAGATCCCTACTCTGGCAGCACCATCTCGCTGTTCCAGGCCATGAAGAAGG GCTTGGTCCTCAGGGACCATGCCATCCGCCTGCTGGAGGCCCAGATTGCCACAGGTGGCATCATTGACCC TGTGCACAGTCACCGCCTGCCCGTAGATGTTGCCTACCAGCGTGGCTACTTCGATGAGGAGATGAACCGT GTGCTGGCTGACCCAAGTGATGACACCAAGGGCTTCTTCGACCCCAACACCCACGAGAACCTCACGTACC TGCAGCTACTGGAGCGCTGCGTGGAGGACCCCGAGACAGGCCTGCGCCTCCTGCCACTCAGAGGGGCAGA GAAGACAGAGGTGGTAGAAACCACACAGGTGTATACTGAGGAGGAGACTCGGAGGGCATTCGAGGAGACG CAGATTGACATCCCAGGTGGTGGCAGCCACGGTGGCTCCTCCATGTCTCTATGGGAGGTGATGCAGTCAG ACATGATCCCAGAGGACCAGCGTGCCCGGCTCATGGCCGACTTTCAGGCTGGCAGAGTGACCAAGGAGCG CATGATCATTATCATCATCGAAATCATTGAGAAGACGGAGATCATCCGCCAGCAGAACCTCGCCTCCTAT GACTACGTACGCCGCCGCCTCACCGCCGAAGACCTGTATGAGGCCCGGATCATCTCCCTTGAGACCTACA ACCTCTTCCGGGAAGGCACCAAGAGCCTCCGTGAGGTTCTGGAGATGGAATCTGCCTGGCGCTACCTTTA CGGCACAGGATCGGTGGCCGGTGTCTACCTGCCTGGCTCTAGGCAGACGCTAACCATCTACCAGGCCCTT AAGAAGGGGCTGCTGAGTGCCGAGGTGGCCCGCTTGCTGCTGGAAGCACAGGCAGCCACAGGCTTTCTGC TGGACCCAGTGAAAGGCGAGAGGCTGACTGTGGACGAGGCCGTGCGGAAGGGTCTGGTAGGCCCCGAGCT GCACGATCGGCTCCTCTCTGCCGAGCGAGCTGTAACTGGCTACCGAGACCCCTACACCGAACAGCCCATC TCACTCTTCCAGGCCATGAAGAAGGAGCTGATCCCTGCCGAGGAGGCACTGAGGCTGCTGGATGCTCAGC TAGCCACAGGAGGCATTGTGGACCCCCGCCTGGGTTTCCACCTCCCCCTGGAGGTGGCTTACCAACGAGG CTACCTCAATAAGGACACGCATGACCAGTTGTCAGAGCCCAGTGAGGTGCGCAGCTATGTGGACCCCTCC ACGGATGAGCGTCTCAGCTACACACAGCTGCTCAAGCGTTGCCGCCGTGACGACAACAGCGGCCAGATGC TGCTGCCGCTCTCTGATGCCCGCAAGCTGACCTTCCGCGGCCTGCGCAAGCAGATCACCGTGGAGGAGCT GGTACGCTCTCAGGTCATGGATGAGGCCACAGCACTGCAGCTGCAAGAAGGCCTGACCTCCATTGAGGAG GTCACTAAGAACCTGCAGAAGTTCCTTGAGGGTACCAGCTGCATTGCTGGAGTCTTTGTTGATGCTACCA AGGAACGGCTGTCGGTGTACCAGGCCATGAAGAAGGGCATCATCCGTCCCGGGACAGCCTTCGAGCTCCT GGAAGCGCAGGCAGCCACCGGCTACGTCATTGACCCTATCAAGGGGCTCAAGCTGACTGTGGAAGAGGCC GTGCGCATGGGTATCGTGGGCCCCGAGTTCAAGGACAAGCTGCTGTCTGCTGAGCGTGCGGTCACTGGCT ACAAGGACCCTTACTCTGGGAAACTCATCTCTCTCTTCCAGGCCATGAAGAAGGGCCTGATCCTGAAGGA CCATGGCATCCGCCTGCTAGAGGCTCAGATCGCCACCGGTGGCATCATTGACCCTGAGGAGAGCCACCGC CTGCCTGTGGAAGTGGCCTATAAGCGTGGTCTCTTTGATGAGGAGATGAACGAGATCCTGACTGACCCCT CAGATGACACCAAGGGCTTCTTCGACCCCAACACCGAGGAGAACCTCACATACCTGCAGCTGATGGAGCG CTGTATCACTGACCCCCAGACTGGCCTGTGTCTCCTGCCGCTGAAGGAAAAGAAGCGGGAGCGGAAGACG TCCTCCAAGTCCTCAGTGCGCAAGCGCCGCGTGGTGATTGTGGACCCTGAGACGGGCAAGGAGATGTCAG TGTATGAGGCCTACCGCAAGGGCCTCATAGACCACCAGACATACCTGGAGTTGTCAGAGCAGGAGTGCGA GTGGGAAGAAATCACCATCTCTTCCTCGGACGGCGTCGTCAAATCTATGATCATCGACCGCCGCTCTGGC CGCCAGTATGACATTGATGACGCCATCACCAAGAACCTCATTGACCGCTCAGCACTGGACCAGTACCGCG CTGGCACACTTTCTATCACCGAGTTTGCCGACATGCTCTCAGGCAACGCTGGTGGCTTCCGCTCCCGCTC CTCCTCTGTGGGCTCATCTTCCTCCTACCCCATCAGTTCTGCTGTCCCTAGGACCCAGCTAGCCTCCTGG TCTGATCCTACTGAGGAGACTGGCCCAGTGGCCGGCATCCTAGACACAGAGACTCTGGAGAAGGTGTCCA TCACAGAGGCCATGCACCGCAACCTGGTAGACAACATCACTGGCCAGCGGTTGCTGGAGGCACAGGCCTG CACCGGGGGCATCATTGACCCCAGCACTGGTGAGCGCTTCCCGGTCACTGAGGCTGTCAACAAGGGCCTG GTGGACAAGATCATGGTAGACCGTATCAATCTGGCCCAGAAGGCCTTCTGTGGGTTTGAGGACCCACGCA CCAAGACCAAGATGTCAGCTGCCCAGGCCCTGAAGAAGGGCTGGCTTTACTACGAGGCAGGCCAGCGTTT CCTCGAGGTGCAGTACCTGACGGGTGGTCTGATTGAGCCTGACACACCTGGCCGTGTGTCTCTTGATGAA GCCTTGCAACGTGGCACTGTGGATGCCCGCACAGCCCAGAAGCTTCGTGATGTCAGTGCCTACTCCAAGT ACCTCACGTGCCCCAAGACCAAGCTCAAGATCTCTTACAAGGACGCTCTGGATCGGAGCATGGTGGAGGA GGGCACAGGGCTGAGGCTGCTGGAAGCCGCGGCACAGTCCAGCAAGGGCTACTACAGCCCGTACAGTGTC AGTGGCTCTGGCTCTACTGCTGGTTCACGCACTGGTTCACGCACCGGCTCCAGGGCCGGCTCCCGTCGTG GCAGCTTTGATGCCACTGGCTCTGGCTTCTCCATGACCTTTTCTTCTTCCTCCTACTCTTCCTCAGGCTA TGGCCGCCGCTATGCCTCAGGGCCTTCAGCCTCTCTTGGGGGCCCTGAGTCTGCAGTGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022401
Insert Size	14064 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_022401.2, NP_071796.2
RefSeq Size	15278 bp
RefSeq ORF	14064 bp
Locus ID	64204
Gene Summary	Plectin is a prominent member of an important family of structurally and in part functionally related proteins, termed plakins or cytolinkers, that are capable of interlinking different elements of the cytoskeleton. Plakins, with their multi-domain structure and enormous size, not only play crucial roles in maintaining cell and tissue integrity and orchestrating dynamic changes in cytoarchitecture and cell shape, but also serve as scaffolding platforms for the assembly, positioning, and regulation of signaling complexes (for reviews see PMID: 9701547, 11854008, and 17499243). Plectin is expressed as several protein isoforms in a wide range of cell types and tissues from a single gene located on chromosome 8 in humans (PMID: 8633055, 8698233). Until 2010, this locus was named plectin 1 (symbol PLEC1 in human; Plec1 in mouse and rat) and the gene product had been referred to as "hemidesmosomal protein 1" or "plectin 1, intermediate filament binding 500kDa". These names were replaced by plectin. The plectin gene locus in mouse on chromosome 15 has been analyzed in detail (PMID: 10556294, 14559777), revealing a genomic exon-intron organization with well over 40 exons spanning over 62 kb and an unusual 5' transcript complexity of plectin isoforms. Eleven exons (1-1j) have been identified that alternatively splice directly into a common exon 2 which is the first exon to encode plectin's highly conserved actin binding domain (ABD). Three additional exons (-1, 0a, and 0) splice into an alternative first coding exon (1c), and two additional exons (2alpha and 3alpha) are optionally spliced within the exons encoding the acting binding domain (exons 2-8). Analysis of the human locus has identified eight of the eleven alternative 5' exons found in mouse and rat (PMID: 14672974); exons 1i, 1j and 1h have not been confirmed in human. Furthermore, isoforms lacking the central rod domain encoded by exon 31 have been detected in mouse (PMID:10556294), rat (PMID: 9177781), and human (PMID: 11441066, 10780662, 20052759). The short alternative amino-terminal sequences encoded by the different first exons direct the targeting of the various isoforms to distinct subcellular locations (PMID: 14559777). As the expression of specific plectin isoforms was found to be dependent on cell type (tissue) and stage of development (PMID: 10556294, 12542521, 17389230) it appears that each cell type (tissue) contains a unique set (proportion and composition) of plectin isoforms, as if custom-made for specific requirements of the particular cells. Concordantly, individual isoforms were found to carry out distinct and specific functions (PMID: 14559777, 12542521, 18541706). In 1996, a number of groups reported that patients suffering from epidermolysis bullosa simplex with muscular dystrophy (EBS-MD) lacked plectin expression in skin and muscle tissues due to defects in the plectin gene (PMID: 8698233, 8941634, 8636409, 8894687, 8696340). Two other subtypes of plectin-related EBS have been described: EBS-pyloric atresia (PA) and EBS-Ogna. For reviews of plectin-related diseases see PMID: 15810881, 19945614. Mutations in the plectin gene related to human diseases should be named based on the position in NM_000445 (human variant 1, isoform 1c), unless the mutation is located within one of the other alternative first exons, in which case the position in the respective Reference Sequence should be used. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (6) differs in the 5' UTR and the 5' coding region compared to variant 1. The resulting protein (isoform 1) contains a distinct N-terminus compared to isoform 1c. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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