Jph2 (NM_001037974) Rat Untagged Clone

CAT#: RN211227

Jph2 (untagged ORF) - Rat junctophilin 2 (Jph2), (10 ug)



  "NM_001037974" in other vectors (3)

CNY 8,930.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN211227 representing NM_001037974
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGGGGGCCGCTTTGACTTTGATGATGGCGGGGCGTATTGTGGGGGCTGGGAAGGGGGGAAGGCAC
ATGGGCACGGACTGTGCACCGGCCCTAAGGGCCAAGGTGAATACTCGGGCTCCTGGAATTTTGGCTTTGA
AGTGGCAGGCGTCTACACCTGGCCCAGTGGGAATACCTTTGAGGGATATTGGAGCCAGGGCAAACGACAT
GGACTTGGCATAGAGACCAAGGGGCGATGGCTCTACAAGGGGGAGTGGACACATGGCTTCAAGGGGCGCT
ACGGAATCCGGCAGAGCACAAACAGTGGTGCCAAGTACGAGGGCACTTGGAATAACGGCCTACAGGATGG
TTATGGCACGGAGACCTATGCAGACGGAGGGACCTACCAAGGCCAATTCACCAACGGCATGCGGCATGGC
TACGGCGTGCGCCAAAGCGTGCCCTACGGGATGGCAGTGGTGGTGCGCTCCCCGCTGCGCACCTCGCTGT
CCTCGCTGCGCAGCGAGCACAGCAATGGCACAGTGGCTCCAGACTCGCCGGCGGCAGACGGGCCCACGCT
GCCCTTGCCCCCGGTGCCGCGTGGTGGCTTCGCGCTCAGCCTACTGGCCACGGCCGAGGCTGCGCGTCCC
CCAGGGCTGTTCACACGAGGCGCACTGCTGGGTCGACTGCGACGCTCAGAGTCACGCACGTCGCTGGGCA
GCCAGCGCAGCCGCTTGAGCTTCCTCAAGAGCGAGCTGAGCTCAGGAGCCAGCGATGCCGCGTCCACCGG
CAGCCTGGCCGAAGGCGCCGAGGGCCCTGACGACGCGGCTGCACCCTTCGACGCCGACATCGACGCCACC
ACCACGGAGACCTACATGGGCGAGTGGAAGAACGACAAACGCTCGGGCTTCGGTGTGAGCGAACGTTCCA
GCGGCCTTCGCTACGAGGGCGAGTGGCTGGACAACCTGCGCCACGGTTACGGTCGCACCACGCTGCCCGA
CGGCCACCGCGAGGAGGGCAAGTACCGCCACAATGTGCTGGTCAAGGGCACCAAGCGCCGCGTGCTGCCG
CTCAAGAGCAACAAGGTTCGCCAGAAGGTGGAGCATGGGGTCGAGGGTGCGCAACGCGCAGCAGCCATTG
CGCGCCAGAAGGCTGAGATTGCTGCCTCCAGGACAAGCCATGCCAAAGCCAAGGCCGAGGCAGCAGAACA
GGCTGCCCTGGCTGCCAACCAGGAGTCCAACATTGCCCGTACATTGGCCAAGGAGCTGGCTCCAGACTTC
TACCAGCCAGGTCCGGAGTATCAGAAGCGTCGGCTGCTCCAGGAGATCCTGGAGAACTCAGAGAGCCTGC
TGGAGCCCCGGGAGCGGGGCCCGGGCACCGGCCTCCCCGAACGGCCCCGGGAAAGCCCACAGCTGCATGA
GCGCGAGACCCCGCAGCCGGAGGGCGGACCCCCTTCCCCGGCCGGAACGCCCCCGCAACCCAAGAGACCC
CGGCCGGGATCGTCAAAGGACGGTCTGCTGAGTCCGGGTGCCTGGAACGGCGAGCCCGGTGGAGAGGGCA
GCCGGCCCGCCACGCCATCGGATGGCGCCGGCCGTCGTAGCCCCGCGCGTCCGGCTTCGGAGCACATGGC
CATCGAGGCGCTGCAGCCACCGCCCGCGCCCTCGCGGGAGCCTGAGGTGGCACTGTACCGCGGTTACCAC
AGCTACGCTGTGCGCACCGGGCCACCCGAGCCCCCGCCCTTGGAGGATGAGCCCGAGCCCGAGCCCGAGG
TCCCGCGATCCGACTCGGAGCCCCCGTCCCCCGTCTCCGCCACGGTCCAGGAAGAGGAGTCCCCTGCGCC
GCGAAGCCGGGTGCCCGCCAAGCCCGCCACCTTGGAGCCCAAGCCCATAGTCCCCAAAGCCGAGCCCAAG
GCCAAGGCGCGCAAGACAGAGGCCCGCGGGCTGAGCAAGGCGGGTGCCAAGAAGAAGGGCCGGAAAGAAG
TGGCACAGGAGGCCGAGGCCGAGGTGGAGGTAGAGGAGGTCCCCAACACCGTCCTCATCTGTATGGTGAT
CCTGCTGAACATCGGCCTGGCTATCCTATTCGTTCACCTCCTGACTTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001037974
Insert Size 2079 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001037974.1, NP_001033063.1
RefSeq Size 2127 bp
RefSeq ORF 2079 bp
Locus ID 296345
UniProt ID Q2PS20
Gene Summary Junctophilin-2: Membrane-binding protein that provides a structural bridge between the plasma membrane and the sarcoplasmic reticulum and is required for normal excitation-contraction coupling in cardiomyocytes. Provides a structural foundation for functional cross-talk between the cell surface and intracellular Ca(2+) release channels by maintaining the 12-15 nm gap between the sarcolemma and the sarcoplasmic reticulum membranes in the cardiac dyads. Necessary for proper intracellular Ca(2+) signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release. Contributes to the construction of skeletal muscle triad junctions.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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