Jph2 (NM_001037974) Rat Untagged Clone
CAT#: RN211227
Jph2 (untagged ORF) - Rat junctophilin 2 (Jph2), (10 ug)
CNY 8,930.00
Product images
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN211227 representing NM_001037974
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGGGGCCGCTTTGACTTTGATGATGGCGGGGCGTATTGTGGGGGCTGGGAAGGGGGGAAGGCAC ATGGGCACGGACTGTGCACCGGCCCTAAGGGCCAAGGTGAATACTCGGGCTCCTGGAATTTTGGCTTTGA AGTGGCAGGCGTCTACACCTGGCCCAGTGGGAATACCTTTGAGGGATATTGGAGCCAGGGCAAACGACAT GGACTTGGCATAGAGACCAAGGGGCGATGGCTCTACAAGGGGGAGTGGACACATGGCTTCAAGGGGCGCT ACGGAATCCGGCAGAGCACAAACAGTGGTGCCAAGTACGAGGGCACTTGGAATAACGGCCTACAGGATGG TTATGGCACGGAGACCTATGCAGACGGAGGGACCTACCAAGGCCAATTCACCAACGGCATGCGGCATGGC TACGGCGTGCGCCAAAGCGTGCCCTACGGGATGGCAGTGGTGGTGCGCTCCCCGCTGCGCACCTCGCTGT CCTCGCTGCGCAGCGAGCACAGCAATGGCACAGTGGCTCCAGACTCGCCGGCGGCAGACGGGCCCACGCT GCCCTTGCCCCCGGTGCCGCGTGGTGGCTTCGCGCTCAGCCTACTGGCCACGGCCGAGGCTGCGCGTCCC CCAGGGCTGTTCACACGAGGCGCACTGCTGGGTCGACTGCGACGCTCAGAGTCACGCACGTCGCTGGGCA GCCAGCGCAGCCGCTTGAGCTTCCTCAAGAGCGAGCTGAGCTCAGGAGCCAGCGATGCCGCGTCCACCGG CAGCCTGGCCGAAGGCGCCGAGGGCCCTGACGACGCGGCTGCACCCTTCGACGCCGACATCGACGCCACC ACCACGGAGACCTACATGGGCGAGTGGAAGAACGACAAACGCTCGGGCTTCGGTGTGAGCGAACGTTCCA GCGGCCTTCGCTACGAGGGCGAGTGGCTGGACAACCTGCGCCACGGTTACGGTCGCACCACGCTGCCCGA CGGCCACCGCGAGGAGGGCAAGTACCGCCACAATGTGCTGGTCAAGGGCACCAAGCGCCGCGTGCTGCCG CTCAAGAGCAACAAGGTTCGCCAGAAGGTGGAGCATGGGGTCGAGGGTGCGCAACGCGCAGCAGCCATTG CGCGCCAGAAGGCTGAGATTGCTGCCTCCAGGACAAGCCATGCCAAAGCCAAGGCCGAGGCAGCAGAACA GGCTGCCCTGGCTGCCAACCAGGAGTCCAACATTGCCCGTACATTGGCCAAGGAGCTGGCTCCAGACTTC TACCAGCCAGGTCCGGAGTATCAGAAGCGTCGGCTGCTCCAGGAGATCCTGGAGAACTCAGAGAGCCTGC TGGAGCCCCGGGAGCGGGGCCCGGGCACCGGCCTCCCCGAACGGCCCCGGGAAAGCCCACAGCTGCATGA GCGCGAGACCCCGCAGCCGGAGGGCGGACCCCCTTCCCCGGCCGGAACGCCCCCGCAACCCAAGAGACCC CGGCCGGGATCGTCAAAGGACGGTCTGCTGAGTCCGGGTGCCTGGAACGGCGAGCCCGGTGGAGAGGGCA GCCGGCCCGCCACGCCATCGGATGGCGCCGGCCGTCGTAGCCCCGCGCGTCCGGCTTCGGAGCACATGGC CATCGAGGCGCTGCAGCCACCGCCCGCGCCCTCGCGGGAGCCTGAGGTGGCACTGTACCGCGGTTACCAC AGCTACGCTGTGCGCACCGGGCCACCCGAGCCCCCGCCCTTGGAGGATGAGCCCGAGCCCGAGCCCGAGG TCCCGCGATCCGACTCGGAGCCCCCGTCCCCCGTCTCCGCCACGGTCCAGGAAGAGGAGTCCCCTGCGCC GCGAAGCCGGGTGCCCGCCAAGCCCGCCACCTTGGAGCCCAAGCCCATAGTCCCCAAAGCCGAGCCCAAG GCCAAGGCGCGCAAGACAGAGGCCCGCGGGCTGAGCAAGGCGGGTGCCAAGAAGAAGGGCCGGAAAGAAG TGGCACAGGAGGCCGAGGCCGAGGTGGAGGTAGAGGAGGTCCCCAACACCGTCCTCATCTGTATGGTGAT CCTGCTGAACATCGGCCTGGCTATCCTATTCGTTCACCTCCTGACTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001037974 |
Insert Size | 2079 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001037974.1, NP_001033063.1 |
RefSeq Size | 2127 bp |
RefSeq ORF | 2079 bp |
Locus ID | 296345 |
UniProt ID | Q2PS20 |
Gene Summary | Junctophilin-2: Membrane-binding protein that provides a structural bridge between the plasma membrane and the sarcoplasmic reticulum and is required for normal excitation-contraction coupling in cardiomyocytes. Provides a structural foundation for functional cross-talk between the cell surface and intracellular Ca(2+) release channels by maintaining the 12-15 nm gap between the sarcolemma and the sarcoplasmic reticulum membranes in the cardiac dyads. Necessary for proper intracellular Ca(2+) signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release. Contributes to the construction of skeletal muscle triad junctions.[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR211227 | Jph2 (Myc-DDK-tagged ORF) - Rat junctophilin 2 (Jph2), (10 ug) |
CNY 8,930.00 |
|
RR211227L3 | Lenti ORF clone of Jph2 (Myc-DDK-tagged ORF) - Rat junctophilin 2 (Jph2), (10 ug) |
CNY 10,640.00 |
|
RR211227L4 | Lenti ORF clone of Jph2 (mGFP-tagged ORF) - Rat junctophilin 2 (Jph2), (10 ug) |
CNY 11,780.00 |