Myh3 (NM_012604) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Myhse; RNMHCG
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211231 representing NM_012604 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAGCGACACCGAGATGGAAGTGTTTGGCATAGCTGCCCCTTTCCTCCGAAAGTCGGAAAAGGAGA GGATCGAAGCTCAGAACCAGCCCTTTGATGCCAAAACCTATTGCTTTGTGGTTGACTCAAAGGAAGAATA TGCCAAGGGGAAAATTAAGAGTAGCCAGGATGGGAAAGTCACGGTGGAGACAGAAGACAACAGGACCCTG GTGGTTAAACCAGAGGACGTGTATGCCATGAACCCTCCCAAGTTTGACAAGATCGAGGACATGGCCATGC TGACCCACCTGAACGAGCCTGCTGTGCTGTACAACCTCAAGGACCGATACACGTCCTGGATGATCTACAC CTATTCAGGCCTCTTCTGTGTCACCGTCAACCCCTACAAGTGGTTGCCGGTGTACACCCCTGAGGTGGTG GATGGCTACCGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGACAACGCCTACC AGTTCATGCTGACGGATCGTGAGAACCAGTCCATCCTGATCACCGGAGAATCCGGGGCCGGGAAGACGGT GAACACCAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGCCACTGGGGACCTTGCCAAGAAGAAGGAC TCCAAAATGAAGGGGACCCTGGAAGATCAAATCATCAGCGCCAACCCCCTGCTGGAGGCCTTTGGGAATG CCAAGACCGTGAGGAATGACAACTCTTCCCGCTTTGGCAAGTTCATCCGCATCCATTTCGGCACCACTGG GAAGCTGGCCTCTGCAGATATTGAAACTTATCTGCTGGAAAAGTCCAGAGTCACCTTCCAGCTGAAGGCT GAGAGAAGCTATCACATCTTCTACCAGATTCTTTCCAACAAGAAGCCGGAACTGATCGAGTTGCTGCTGA TTACGACCAACCCTTACGACTACCCATTCATCAGCCAGGGTGAAATCCTGGTGGCCAGCATCGATGATCG TGAGGAACTGTTGGCTACAGACAGTGCCATTGACATCCTGGGCTTCACCCCGGAGGAGAAATCTGGACTC TATAAACTGACAGGGGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAGAAGCAGCGAGAGGAGCAAG CTGAGCCAGATGGCACCGAAGTGGCTGACAAAACAGCCTACTTGATGGGCCTGAACTCTTCAGACCTCCT CAAAGCCTTGTGCTTTCCCAGAGTGAAAGTTGGGAATGAGTACGTTACAAAAGGCCAAACTGTGGACCAG GTTCACCATGCTGTGAATGCTCTCTCCAAGTCCGTTTATGAGAAGCTGTTCTTGTGGATGGTCACTCGTA TTAACCAGCAGCTGGACACAAAGCTACCAAGACAACACTTCATTGGGGTTTTGGACATTGCGGGTTTTGA AATTTTTGAGTATAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAGTTTTTC AACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACCTTCATCGACT TCGGGATGGACCTGGCGGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCTGGAAGA GGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCACCTTGGAAAA TCCAACAACTTCCAGAAGCCTAAGGTGGTCAAAGGCAAGGCCGAGGCCCACTTCTCCCTCATCCACTATG CGGGCACCGTGGACTACAGTGTCTCGGGCTGGCTGGAGAAGAACAAGGACCCCCTGAATGAGACCGTGGT GGGGCTGTACCAGAAGTCTTCCAACAGACTCCTGGCCCATCTCTATGCCACCTTCGCTACGACAGATGCT GACGGTGGGAAGAAGAAAGTCGCTAAGAAGAAAGGCTCTTCCTTCCAAACAGTGTCTGCGCTGTTCAGGG AAAACTTGAACAAGCTGATGTCCAATTTAAGAACCACTCACCCACACTTTGTGCGCTGTATAATACCCAA TGAAACCAAGACCCCAGGGGCCATGGAACACAGCCTGGTCCTGCACCAGCTGAGGTGTAACGGGGTGCTA GAAGGCATCCGCATCTGCAGGAAGGGGTTCCCCAACAGGATTCTGTATGGAGACTTTAAACAGAGATACC GAGTGCTGAACGCCAGTGCCATCCCGGAGGGCCAGTTCATTGACAGCAAGAAAGCTTGCGAGAAGCTCTT GGCTTCCATTGACATCGACCACACACAGTACAAATTTGGACACACCAAGGTGTTCTTTAAGGCTGGCTTG CTGGGAACCCTGGAGGAGATGCGGGATGAGCGTCTGGCCAAACTGATCACTCGGACACAAGCCGTGTGCA GAGGGTTCCTCATGCGTGTGGAATTCCAGAAGATGATGCAGAGGAGGGAATCCATCTTCTGCATCCAGTA CAACATCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAGCCCCTG CTGAAGAGCGCCGAGACGGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCCAGAAGACCAAGGACGAGC TCGCCAAGTCGGAGGCAAAGAGGAAGGAGCTGGAGGAGAAGCTGGTCACTCTGGTACAAGAGAAGAACGA CCTGCAGCTCCAAGTACAGGCGGAGAGTGAGAATTTGCTGGATGCCGAGGAGCGATGCGACCAGCTGATC AAAGCCAAGTTCCAGCTGGAGGCCAAAATTAAAGAGGTGACCGAAAGAGCTGAGGATGAGGAGGAGATCA ACGCCGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCGGAGCTGAAGAAGGACATCGATGA CCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAACAAGGTAAAAAACCTTACC GAAGAACTGGCAGGCCTGGATGAAACTATTGCAAAGCTAACACGGGAGAAGAAGGCCCTCCAAGAGGCCC ACCAGCAGACCCTGGACGACCTCCAGGCTGAGGAAGACAAGGTCAATTCTCTGAGCAAGCTCAAGAGCAA GCTGGAGCAGCAAGTGGATGATTTGGAGAGCTCCCTCGAACAAGAGAAGAAGCTGCGTGTGGACCTGGAA AGGAACAAGAGGAAGCTGGAGGGAGACCTGAAGCTTGCCCAAGAGTCCATATTAGATCTGGAGAACGACA AGCAACAGCTGGACGAGAGGCTCAAGAAGAAGGACTTTGAATATAGCCAGCTGCAGAGCAAGGTGGAAGA TGAGCAGACGCTGAGCCTCCAACTTCAGAAGAAAATCAAAGAGTTACAGGCCCGCATTGAGGAGCTGGAG GAAGAGATAGAGGCGGAGAGGGCCACCCGGGCCAAGACAGAGAAGCAGCGCAGCGACTACGCCCGCGAGC TGGAGGAGCTGAGTGAACGTCTGGAGGAGGCGGGCGGTGTCACCTCTACCCAGATTGAGCTGAACAAGAA GCGTGAGGCTGAGTTCTTGAAGCTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAGGCCACCGTG GCCACGCTGCGGAAGAAGCACGCGGACAGCGCGGCGGAGCTGGCAGAGCAGATCGACAACCTGCAGCGCG TCAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGATCGACGACCTCTCCAGCAGTGTGGA GAGTGTGTCCAAATCCAAGGCCAACCTGGAGAAGATATGCCGAACTCTAGAGGATCAGTTAAGCGAGGCC AGGGGCAAGAACGAGGAGACGCAGAGAAGCCTAAGTGAGCTGACCACACAGAAGTCCCGGCTGCAGACAG AAGCGGGTGAGCTGAGTCGTCAACTGGAAGAAAAGGAAAGCATAGTATCTCAGCTTTCCAGGAGCAAGCA AGCCTTTACCCAACAGATAGAGGAGCTCAAGAGGCAACTGGAGGAAGAGAACAAGGCCAAGAATGCGCTG GCCCACGCCCTGCAGTCCTCCCGCCACGACTGTGACCTGCTTCGGGAACAGTACGAGGAGGAGCAGGAAG GCAAAGCCGAGCTGCAGAGGGCGCTGTCCAAGGCCAACAGCGAGGTGGCCCAATGGAGGACCAAATATGA GACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAGGCCAAAAAAAAGCTTGCTCAGCGCCTTCAGGAT TCGGAGGAGCAGGTCGAGGCTGTGAATGCAAAATGCGCTTCCTTGGAAAAGACCAAGCAGAGGCTGCAAG GGGAGGTGGAGGACTTGATGGTGGATGTGGAGAGAGCCAACTCTCTGGCTGCCGCTCTGGACAAGAAGCA GAGGAACTTTGACAAGGTGTTAGCCGAGTGGAAGACGAAGTGTGAGGAGAGCCAAGCAGAGCTGGAGGCG GCTCTCAAGGAATCCCGGTCCTTGAGCACTGAGCTCTTCAAACTGAAAAACGCCTATGAAGAAGCCTTGG ATCAACTCGAAACTGTGAAGCGAGAGAATAAGAACTTAGAACAAGAGATAGCAGATCTCACCGAACAAAT TGCTGAGAACGGTAAAAGCATCCACGAGCTAGAGAAATCCAGAAAGCAGATGGAGCTGGAGAAGGCTGAT ATCCAGATGGCGCTGGAGGAAGCAGAGGCAGCTCTGGAGCACGAAGAAGCCAAGATCCTCAGGATCCAGC TTGAACTGACGCAGGTGAAATCAGAGATCGATAGAAAGATCGCAGAGAAGGATGAAGAGATCGAGCAGCT GAAGAGGAACTACCAGAGGACAGTGGAGACCATGCAGGGCGCCCTGGATGCTGAGGTGCGCAGCAGGAAT GAGGCGATCCGGCTGAAGAAGAAGATGGAGGGCGACCTCAATGAGATTGAGATCCAGCTGAGCCATGCCA ACCGCCAGGCCGCAGAGACCATCAAGCACCTCCGGAGTGTCCAGGGACAGCTGAAGGACACCCAGCTGCA CCTGGATGACGCTCTCCGAGGCCAGGAGGACCTGAAGGAGCAGCTGGCGATCGTGGAGCGCAGAGCCAAC CTGCTGCAGGCTGAGGTGGAGGAGCTGCGGGCCACTCTGGAGCAGACGGAGAGGGCCCGGAAGCTGGCAG AGCAGGAGCTGCTGGACTCCAACGAGAGGGTTCAGCTGCTGCACACCCAGAACACCAGCCTCATCCACAC CAAGAAGAAGTTGGAGACTGACCTCACACAGCTCCAGAGTGAGGTGGAGGATGCCAGCAGGGACGCAAGG AACGCCGAGGAGAAGGCCAAAAAGGCCATCACTGATGCCGCCATGATGGCGGAGGAGCTAAAGAAGGAGC AGGACACCAGCGCCCATCTGGAGCGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCTGCAGCACCG TCTGGACGAGGCTGAGCAGCTGGCACTGAAGGGCGGCAAGAAGCAGATCCAGAAACTGGAGACGAGGATC AGAGAGCTAGAGTTTGAGCTGGAAGGGGAACAGAAGAGGAACACAGAGTCTGTGAAGGGCCTGAGGAAGT ATGAGCGGCGTGTCAAGGAGCTCACCTACCAGAGTGAGGAGGACAGGAAGAATGTGCTGAGATTGCAGGA TCTGGTGGATAAACTTCAAGTGAAAGTCAAGTCCTACAAGAGGCAGGCTGAGGAGGCTGATGAACAAGCC AATGTTCATCTCACCAAGTTCCGGAAAGCCCAGCATGAGCTAGAGGAGGCCGAGGAACGTGCGGATATTG CAGAATCGCAAGTCAATAAACTGCGGGCTAAAACCCGGGACTTCACCTCTAGCCGGATGGTGGTCCATGA AAGTGAGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_012604
Insert Size	5823 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_012604.1, NP_036736.1
RefSeq Size	6035 bp
RefSeq ORF	5823 bp
Locus ID	24583
UniProt ID	P12847
Gene Summary	heavy chain of myosin; involved in muscle contraction [RGD, Feb 2006]

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