Brca2 (NM_031542) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211711 representing NM_031542 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGTTGAATACAAAAGGAGACCAACTTTTTGGGAAATTTTTAAAGCGAGATGCAGCACAGCAGATT TAGGACCAATAAGCCTCAATTGGTTTGAAGAACTTTCTTCAGAAGCCCCACCATATAATACTGAACCTCC AGAAGAATCTGAATATAAACCCCAAGGCCATGAACCACAGCTATTTAAAACACCACAGAGGAATCCCTCT TACCATCAGTTTGCTTCAACTCCAATAATGTTCAAAGAACAAAGTCAAACTCTACCACTAGACCAGTCAC CTTTCAAAGAATTAGGGAATGTTGTTGCAAATAGTAAACGTAAACATCACAGCAAAAAGAAGGCCAGAAA GGACCCTGTGGTAGATGTTGCCAGTCTGCCGCTGAAAGCTTGTCCCAGTGAAAGCCCTTGTACTCCGCGA TGCACACAGGTGGCACCGCAGCGAAGAAAGCCAGTGGTATCTGGAAGTTTATTCTATACACCAAAACTTG AGGAGACACCAAAACATATTTCTGAAAGTCTGGGAGTTGAAGTGGATCCGGATATGTCTTGGACAAGTTC ATTAGCTACACCACCAACCCTTAGTTCCACTGTGCTCATAGCCCGAGATGAAGAAGCACACAGAAATGCA TTTCCAGCCGACTCTCCTGCTAGTTTGAAAAGCTATTTTTCTAACCACAATGAAAGTCTGAAAAAGAATG ATAGATTTATTCCCTCTGTGTCTGACAGTGAAAACAAAAGCCAGCAAGAAGCTTTTAGTCAGGGACTGGA GAAAATGTTAGGGGATTCATCTAGCAAAATAAATCGCTTCAGAGACTGCCTTAGAAAACCAATACCAAAT GTTCTAGAAGATGGGGAGACAGCTGTAGATACTTCAGGAGAAGATAGTTTCTCGTTATGTTTTCCTAAAC GTAGAACCAGAAATCTACAGAAAACGAGAATGGGCAAGATGAAGAAGAAAATCTTCAGTGAAACAAGGAC TGATGGATTAAGTGAAGAAGCCAGAGGACAAGCTGATGATAAAAACTCATTTGCACTTGAAATTGAACCA AGAGATAGTGAGCCCTTAGATCCATCTGTAACAAACCAGAAGCCCCTTTACAGCCAGAGTGGGGACATCT CCAGTGAAGCTGGCCAGTGTTCAGACAGTATATGGTCTCAGCCAGATCCCTCTGGTCTAAATGGAACCCA GACAAGAAAAATACCCCTACTCCATATTTCTTCTCATAAGCAAAGTATTTTAGAAGACTTCATAGATATG AAGAAGGAAGGTACTGGCTCTATTACTTTTCCTCATATTTCTAGTCTTCCAGAACCAGAAAAGATGTTCA GTGAGGAAACTCTGGTAGACAAGGAACATGAAGGACAGCATCTTGAGTCACTTGAAGACTCCATCTCAGG GAAACAAATGGTGTCTGGAACGAGTCAAACAGCCTGTCTCTCTCCGAGTATCAGGAAGTCTATAGTCAAG ATGAGAGAGCCACTTGAAGAGACTTTGGATACTGTTTTCTCAGACAGTATGACCAGCTCAGCCTTTACAG AAGAACTTGACGCCTCTGCAGGGGGACTGGAAATACATACTGCATGCTCACAGAGAGAGGATTCTTTATG TCCTAGTTCAGTGGACACTGGAAGCTGGCCAACCACTCTCACTGACACTTCTGCAACTGTGAAAAATGCA GGTTTAATAACCACTCTCAAAAATAAAAGAAGAAAGTTTATTTACTCTGTAAGTGATGATGCATCTCATC AAGGAAAAAAACTACAGACACAGAGACAGTCAGAGCTTACTAACCTTTCAGCCCCGTTTGAAGCAAGTGC TTTTGAAGTACCATTCCCCTTTACAAATGTAGATTCAGGTATACCAGATTCTTCAATCAAAAGAAGCAAT TTACCAAATGATCCTGAAGAGCCATCTTTGTCCTTGACCAACTCTTTTGTGACTGCTGCCAGTAAAGAAA TTAGTTATATTCATGCATTGATATCTCAGGATCTAAATGACAAAGAAGCAATACTCAGTGAAGAAAAGCC ACAGCCATATACAGCCCTAGAAGCTGACTTTCTGTCGTGCTTGCCAGAAAGATCATGTGAAAATGATCAA AAAAGTCCAAAAGTTTCCGACCGAAAAGAAAAAGTCTTAGTCTCAGCATGTCGTCCTTCAGGAAGGCTTG CAGCAGCAGTGCAGCTCAGCAGCATTAGCTTTGACTCTCAGGAAAACCCTCTTGGTAGCCACAACGTAAC AAGTACTCTTAAATTAACTCCCAGCCCGAAGACACCTCTGTCAAAGCCAGTTGTGGTTTCTAGAGGGAAA ATGTGTAAAATGCCAGAGAAACTGCAATGTAAGAGTTGTAAAGATAATATTGAATTAAGCAAAAACATCC CTCTGGGGGTTAATGAAATGTGTGTCTTAAGTGAAAATTCTGAAACACCTGAGCTTCTGCCACCTCTAGA ATATATAACAGAAGTGTCCTCATCAGTGAAGTCACAGTTCAATCAAAATACAAAAATAGCAGTCGTACAA AAGGACCAAAAAGACTCAACTTTTATTTCAGAAGTAACAGTCCATATGAATTCTGAAGAACTTTTCCCAG AAAAGGAGAATAATTTTGCTTTTCAAGTAACCAATGAAAGCAATAAACCCAATATAGGAAGTACTGTGGA ATTCCAGGAAGAAGACCTCAGCCACACAAAAGGGCATAGTCTCAAGAACTCTCCCATGACAGTAGATAGA GACCTAGATGATGAGCAAGCAGGCCAAGTGTTGATTACAGAGGACTCAGATTCATTAGCAGTAGTCCATG ATTGTACAAAGAAGAGCAGAAATACTATAGAGCAGCATCAGAAAGGAACTGCAGACAAAGACTTCAAGTC AAATTCCTCCTTGTATTTGAAATCAGATGGGAACAATGATTATTTAGACAAATGGTCAGAGTTCTTGGAT CCACTCATGAACCATAAATTTGGAGGTAGCTTCAGAACAGCTTCCAATAAAGAAATAAAACTTTCAGAGG ATAATGTCAAGAAAAGCAAAATGTTCTTCAAAGATATCGAAGAACAGTATCCTACTAGTTTAGATTGTAT TGACACTGTTAGTACCCTACAATTAGCAAACAAGAAGAGACTAAGTGAACCTCATACATTTGATTTGAAG TCAGGTACTACTGTATCTACACAGTGTCATAGTCAATCATCTGTTTCTCATGAAGATACTCACACAGCAC CTCAGATGTTATCTTCAAAGCAAGATTTTCATTCAAGTCATAACTTAACGCCCAGCCAAAAAGCAGAAAT TACAGAACTGTCTACTATCTTGGAAGAATCAGGAAGTCAGTTTGAATTCACACAGTTCAAAAATCCAAGC CACATAGCACAGAATAATACATCTGCAGTGCTTGGAAACCAGATGGCTGTTGTAAGGACCGCTTCTGAGG AGTGGAAAGATGTTGATCTTCATCTCCCACTGAATCCCTCCTCTGTAGGTCAGATAGATCACAACAAGAA ATTCGAATGTTTGGTTGGAGTTAAGCAAAGCTCTTCTCACCTGTTAGAAGACACTTGTAACCAAAATACA TCTTGTTTTTTACCGATAAAAGAAATGGAGTTTGGAGGATTTTGTTCTGCTCTTGGCACAAAACTTAGTG TGTCTAATGAGGCTCTGAGAAAGGCTATGAAACTGTTCAGTGACATTGAAAATATTAGTGAGGAGCCTTC TACAAAAGTAGGACCAAGAGGATTCTCTTCATGTGCACATCATGATTCTGTTGCTTCCGTGTTTAAGATA AAGAAACAAAACACTGATAAAAGTTTTGATGAGAAATCTAGTAAGTGCCAGGTAACAGTACAAAATAATA AGGAAATGACTACCTGTATTCTTGTTGATGAAAATCCTGAAAATTATGTAAAGAATATAAAACAAGATAA CAACTATACTGGCTCTCAAAGAAATGCTTATAAATTAGAAAACTCTGATGTTAGTAAATCAAGTACAAGT GGCACAGTTTATATTAATAAAGGTGACAGTGATTTACCTTTTGCTGCTGAAAAAGGCAATAAGTATCCTG AGTCATGTACCCAATATGTGAGGGAAGAAAATGCACAAATTAAGGAAAGTGTATCAGATTTAACATGTTT GGAAGTCATGAAAGCTGAGGAAACATGTCATATGAAATCTTCAGATAAAGAACAATTACCTTCAGATAAG ATGGAACAAAATATGAAAGAGTTTAATATATCTTTTCAGACTGCAAGCGGGAAAAATATCAGAGTCTCCA AAGAGTCACTAAATAAAAGTGTGAATATTTTAGATCAGGAAACAGAAGACTTGACTGTCACTTCAGATTC TTTGAATTCTAAAATTCTTTGTGGCATAAATAAGGACAAAATGCATATTTCATGTCACAAGAAATCAATC AATATTAAAAAGGTATTTGAAGAACATTTCCCAATTGGAACTGTCAGTCAATTACCAGCTCTTCAGCAGT ATCCTGAATATGAAATAGAAAGTATCAAAGAACCTACTCTGTTGAGTTTTCATACAGCTAGTGGGAAAAA AGTCAAAATTATGCAGGAATCTTTGGACAAAGTGAAAAATCTTTTTGATGAGACACAGTATGTTAGGAAA ACCACCAATTTTGGTCATCAAGAATCAAAACCCCTGAAGGACAGAGAGGACTATAAAGAAAGACTTACAT TAGCATATGAGAAAATTGAAGTAACTGCCTCAAAATGTGAAGAAATGCAGAACTTTGTCTCTAAGCAGAC TGAAATGCTACCCCAGCAAAATGATCATATGTATAGGCAAACTGAAAATCTCACATCAAATGGTAGCTCT CCCAAAGTACATGGAAACATAGAAAATAAAATAGAAAAGAATCCTAGAATTTGCTGTATTTGTCAGTCCT CATACTTTGTCACTGAAGATTCTGCTTTGGCATGTTATACGGGGGACAGTAGAAAAACTTGTGTCGGAGA GTCTTCTCTGTCCAAAGGCAAAAAATGGCTTAGAGAACAAAGTGATAAGCTTGGAACAAGAAATACTATT GAAATCCAATGTGTAAAGGAACACACTGAAGATTTTGCAGGAAATGCCTTATATGAACATAGTTTAGTCA TTATCAGAACTGAAATTGATACAAGTCATGTCTCTGAAAACCAAGCTTCAACCCTCTTTAGTGACCCTAA TGTGTGTCACAGCTATCTATCCCATTCTAGTTTTTGTCATCATGATGATATGCATAATGATTCAGGATAT TTCTTAAAAGATAAAATTGATTCTGATGTCCAGCCAGACATGAAGAATACTGAAGGCAATGCCATTTTCC CTAAAATATCTGCTACAAAAGAAATAAAACTACACCCACAAACTGTAAATGAAGAGTGTGTTCAAAAACT GGAGACTAATGCTTCACCATATGCAAATAAAAATATAGCCATTGACTCAGCTATGCTGGATTTAAGGAAT TGTAAGGTAGGCTCACCTGTATTCATTACAACTCATTCACAAGAAACTGTAAGAATGAAAGAGATATTCA CAGATAACTGTAGTAAAATAGTCGAACAAAACAGGGAGAGTAAACCAGACACTTGCCAGACAAGCTGTCA TAAAGCATTGGATAATTCAGAGGATTTTATATGTCCTAGCTCTTCAGGTGATGTCTGCATAAACTCACCT ATGGCTATTTTTTATCCTCAAAGTGAACAAATTTTACAGCATAACCAAAGTGTGTCTGGACTGAAGAAAG CTGCAACACCACCTGTTAGTTTGGAAACTTGGGATACATGTAAATCTATAAGAGGATCTCCTCAGGAAGT CCATCCTTCACGCACTTATGGAATTTTTAGCACAGCAAGTGGAAAAGCTGTACAAGTATCTGATGCTTCA TTGGAAAAGGCACGGCAAGTGTTTTCTGAGATAGATGGTGATGCTAAACAGTTAGCTTCCATGGTGTCAC TGGAAGGTAATGAAAAATCACATCACTCTGTGAAAAGAGAAAGCTCTGTGGTGCATAACACCCATGGTGT ATTGTCACTCCGAAAAACCCTCCCAGGCAATGTCAGTTCATCTGTATTCTCTGGATTTAGCACCGCAGGT GGAAAACTGGTCACAGTTTCAGAAAGTGCTTTACATAAAGTTAAAGGAATGTTAGAGGAATTTGATTTGA TCAGAACTGAACATACTCTCCAGCATTCACCTACACCTGAAGACGTATCAAAAATACCTCCTCAACCTTG TCTTGAAAGCAGAACCCCAGAATACTCTGTAAGCTCTAAATTGCAGAAAACCTACAATGATAAATCCAGG TCACCAAGTAATTATAAAGAAAGTGGTTCTTCAGGCAATACTCAATCTCTTGAAGTTTCTCCCCAACTCT CTCAGATGGAGAGAAAGCAAGAAACACAGTCGGTATTAGGAACAAAAGTGTCCCAGAGGAAGACTAATAT CTTGGAAAAAAAACAAAACTTACCCCAAAACATAAAAATAGAAAGTAATAAAATGGAAACATTTTCTGAT GTTTCCATGAAAACAAATGTAGGAGAGTACTACTCCAAAGAGCCAGAGAACTATTTTGAAACAGAAGCAG TGGAAATTGCCAAAGCTTTTATGGAAGATGATGAGCTGACGGATTCTGAACAGACTCATGCCAAATGCTC ACTGTTTGCATGCCCCCAAAACGAGGCTTTATTAAATTCGAGAACTAGAAAAAGAGGAGGAATGGCTGGT GTTGCAGTTGGACAACCCCCAATTAAAAGGAGCTTATTAAATGAATTTGACAGGATAATAGAAAGTAAAG GAAAATCCTTAACGCCTTCAAAAAGCACTCCAGATGGCACAATAAAAGACAGACGATTGTTCACACACCA CATGTCTTTAGAGCCGGTTACCTGTGGACCCTTCTGCTCAAGTAAAGAAAGGCAAGAAACCCAGAGCCCA CATGTTACCTCACCTGCTCAAGGACTTCAGTCTAAGGGGCATCCTTCAAGACACTCAGCTGTGGGAAAGT CTTCAAGCAATCCTACAGTTTCTGCCCTAAGATCTGAAAGGACCAGACACTCAGTCTCAGACAAATCCAC CAAAGTCTTTGTCCCACCTTTCAAGGTGAAATCACGGTTTCACAGAGATGAACATTTTGATAGCAAGAAT GTTAATTTGGAGGGAAAAAACCAAAAGAGCGCAGATGGAGTCAGTGAAGATGGGAACGACAGTGACTTTC CTCAGTTTAACAAAGATTTAATGTCAAGCCTTCAGAATGCCAGAGACCTACAGGATATACGAATTAAAAA CAAAGAAAGGCATCATCTCTGTCCGCAGCCAGGCAGTCTGTATCTTACAAAATCATCCACCCTGCCCCGG ATTTCTCTGCAGGCCGCAGTAGGAGACAGCGTTCCTTCTGCCTGTTCTCCTAAGCAGCTCTATATGTATG GCGTTTCTAAAGCATGCATAAGCGTTAACAGCAAAAACGCTGAGTATTTTCAGTTTGCCATTGAGGATCA CTTTGGTAAAGAAGCTCTGTGCGCTGGCAAAGGCTTTCGGTTGGCAGATGGTGGATGGCTAATCCCCTCC GATGACGGGAAGGCTGGGAAAGAAGAATTTTACAGGGCTCTGTGTGACACTCCAGGTGTGGATCCAAAGC TGATTTCTAGCGTCTGGGTCTCTAACCACTACAGGTGGATTGTATGGAAACTGGCAGCTATGGAATTTGC TTTTCCTAAGGAATTCGCGAATAGATGTCTAAACCCAGAGCGAGTGCTGCTTCAACTAAAATACAGATAC GATGTGGAAATTGACAATAGCAGCAGATCAGCTCTCAAGAAGATACTGGAAAGGGATGATACAGCAGCAA AAACGCTTGTCCTCTGTGTTTCTGATATCATTTCACTAAGCACAAATGTGTCTGAAACTTCAGGCAGTAA AGCTAGCAGTGAGGACAGTAACAAAGTAGACACGATCGAACTCACAGATGGGTGGTATGCTGTCAAGGCC CAGCTAGACCCTCCACTCCTGGCTCTCGTAAAGAGTGGGAGACTCACTGTGGGTCAGAAGATCATTACTC AGGGAGCAGAGCTGGTGGGCTCTCCCGATGCCTGTGCACCTCTGGAAGCCCCAGACTCCCTTAGGCTAAA GATTTCTGCAAACAGCACGCGGCCTGCTCGCTGGCACAGCAAGCTGGGGTTCTTTCATGACCCCAGGCCC TTCCCTCTGCCCTTGTCCTCACTGTTCAGTGATGGAGGAAATGTTGGTTGTGTGGATGTCATCGTTCAGA GAGTGTACCCTCTACAGTGGGTGGAGAAGACGGTGTCTGGATCGTACATATTTCGTAATGAGAGAGAGGA GGAGAAGGAAGCGCTGAGATTTGCAGAGGCCCAGCAGAAGAAACTAGAGGCCTTGTTCACCAAAGTCCAC ACAGAGCTTAAAGAGCATGAAGAAGACATAGCCCAGCGGCGTGTGCTGTCCCGGGCACTCACACGGCAGC AGGTCCACGCTCTGCAGGACGGTGCAGAGCTCTATGCAGCAGTGCAGGATGCATCAGACCCAGAGCACCT GGAGACTTGTTTCAGCGAAGAGCAGCTGAGAGCCTTGAACAACTACAGACAAATGCTGAGCGATAAGAAA CAAGCGCGGATCCAGTCAGAGTTCCGGAAGGCCCTGGAGGCCGCTGAGAAAGAAGAGGGTTTATCAAGGG ACGTCTCAACTGTGTGGAAGCTTCGTGTTACAAGCTACAAGAAAAGAGAAAAATCAGCTCTGTTGAGTAT CTGGCGTCCATCTTCAGACTTGCCCTCCCTGTTAACAGAAGGACAGAGATACAGAATCTATCATCTTTCG GTGTCAAAATCGAAGAATAAATTTGAGTGGCCCAGCATCCAGTTAACAGCCACAAAGAGAACTCAGTATC AACAGCTGCCGGTTTCCAGTGAGACCCTGCTCCAGCTTTACCAGCCCAGGGAGCTCCTTCCCTTCAGCAA ACTGTCAGATCCAGCCTTCCAGCCACCTTGTTCTGAAGTGGATGTAGTAGGAGTTGTAGTTTCTGTTGTA AAACCAATAGGTCTTGCTCCTTTGGTCTACTTGTCAGACGAGTGCCTTCATTTATTAGTGGTGAAATTTG GAATAGACCTTAATGAAGACATAAAGCCACGTGTGCTTATTGCTGCGAGCAACCTCCAGTGGCGGCCGGA GTCCACGTCACGAGTGCCAACTTTATTTGCTGGGAACTTTTCCGTATTCTCTGCCAGTCCAAAGGAGGCC CACTTTCAGGAGAGGGTCACGAACATGAAGCATGCTATTGAGAACATCGACACATTTTACAAGGAGGCAG AAAAGAAGCTTATACAGGTGCTGAAGGGAGACAGTCCAAAGTGGTCCACCCCGAACAAAGACCCCACCCG AGAACCCTACCCAGCCTCCACTTGCTCTGCTTCAGACCTTGCTTCAGGAGGTCAGTTACCGAGGAGTTCA CCTACTGATCAGCAAAGTTATCGAAGTCCTTTATCATGCTGCACACCGACGGGGAAATCTACACCCCTGG CCCACTCAGCCTGGATGGCAGCCAAGTCTTGTAGTGGGGAGAATGAGATTGAGGACCCCAAGACCTGTCG AAAAAAAAGGGCCTTGGATCTCCTAAGTCGGCTGCCCTTACCGCCACCGCTCAGTCCTGTCTGCACCTTT GTCTCTCCGGCTGCACAGAAGGCCTTTCAGCCACCACGGAGCTGTGGCACCAAATACCCAACACCTCTAA AAAAAGAGGGACCCAGTTCCCCTTGGAGCAGGGCGCCATTTCAGAAGGCCAGTGGCGTTTCTCTCCTGGA CTGTGATTCAGTAGCTGATGAAGAACTTGCCTTGCTCAGTACCCAAGCCCTTGTGCCTCACTCAGTGGGA GGAAGTGAACAAGTGTTTCCCAGTGATTCCACAAGGACAGAAGGACCCTCAGCCAGCACAGAGGCCAGAC CAGCAAATAGATCCAAGAGGGAGTCTCTGAGGGACTGTAGAGATGACAGCGATGGGAAATTGGCTGCTGA GACAGTTCCAGACTACTCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_031542
Insert Size	10032 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_031542.2, NP_113730.2
RefSeq Size	10350 bp
RefSeq ORF	10032 bp
Locus ID	360254
Gene Summary	tumor suppression gene; involved in repair of double-strand DNA breaks (DSBs) by homologous recombination [RGD, Feb 2006]

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