Prex1 (NM_001135718) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	RGD1306534
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211736 representing NM_001135718 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGCCCGGCAGCGGTAGTGGCGATGGCGGCGGGGACCCAGGCGGGGACGGTGCGCATCCCGATG CTCGCGGTCCCAGCTCCGGGCCATGCGCGGCCGCCCGCGACTCGGAGCGCCAGCTGCGCCTCCGTCTGTG CGTACTCAACGAGATCCTGGCCACCGAGCGCGACTACGTGGGCACCTTGCGTTTCCTGCAGTCGGCGTTC CTACAGCGGATCCGGCAGAATGTGGCCGACTCTGTGGACAAGGGCCTCACGGAAGAGAATGTCAAGATCC TCTTCTCCAACATTGAGGACATCTTGGAGGTCCATAAGGATTTTTTGGCTGCCCTGGAATACTGCCTACA TCCGGAGCCCCAGTCTCAGCACGAACTTGGGAATGTTTTCTTGAAATTTAAGGACAAGTTCTGTGTGTAT GAGGAGTACTGCAGTAACCACGAGAAGGCCCTGCGTCTGTTGGTGGAGCTCAACAAGGTCCCTGCCGCTC GCGCCTTCCTCCTGAACTGCATGCTTCTGGGAGGCCGGAAGACCACGGACATCCCTCTGGAGGGCTACCT GCTGTCCCCCATCCAGAGGATCTGCAAGTACCCACTCCTGCTCAAGGAGTTATCTAAGAGGACCCCGGGC AAACACCCTGACCATTCCGCGGTACAGAGCGCCCTGCAAGCCATGAAGACCGTCTGCTCCAACATCAATG AGACCAAGAGGCAGATGGAGAAGCTGGAGGCCCTGGAGCAGCTGCAGTCTCACATCGAAGGCTGGGAGGG CTCGAACCTCACAGACATCTGCACCCAGCTTCTCCTTCAGGGCACTCTGCTGAAGATCTCTGCAGGCAAC ATCCAAGAGAGAGCCTTCTTCCTCTTCGACAACTTGCTCGTCTACTGCAAGCGGAAATCTAGGGTCACTG GGAGCAAGAAATCTACCAAGAGGACCAAGTCCATCAACGGCTCCCTGTACATCTTCAGGGGTCGGATCAA CACTGAGGTCATGGAAGTGGAGAATGTGGAAGACGGGACAGCCGATTACCACAGCAATGGCTACACCGTC ACCAATGGCTGGAAGATCCACAATACAGCCAAAAACAAGTGGTTTGTGTGTATGGCCAAGACAGCAGAGG AGAAGCAGAAGTGGCTGGACGCCCTGATCCGAGAGCGGGAGCAGCGAGAGAGCCTGAAGCTGGGCATGGA GCGCGACGCATACGTCATGATTGCGGAGAAGGGGGAGAAGCTGTATCACATGATGATGAGCAAGAAGGTG AACTTGATCAAGGACCGCCGACGGAAGCTGAGCACCGTCCCCAAGTGCTTCCTTGGCAATGAGTTTGTCG CCTGGCTCCTGGAAATTGGTGAGATCAGCAAGACAGAAGAAGGGGTCAACTTGGGCCAGGCCCTGTTGGA GAATGGCATCATCCACCACGTCTCCGACAAGCATCAGTTCAAGAACGAGCAGGTGATGTATCGCTTCCGC TACGACGATGGCACCTACAAAGCCCGCAGCGAGCTGGAGGACATCATGTCCAAGGGCGTGAGACTCTATT GCCGGCTTCACAGCCTGTACGCCCCCGTGATCAAGGACCGTGATTACCACCTGAAGACCTACAAGTCCGT GGTCCCCGGGAGCAAGCTGGTCGACTGGCTCCTGGCACAGGGGGACTGCCAGACACGGGAGGAGGCGGTG GCGCTGGGTGTGGGCCTGTGCAACAACGGTTTCATGCACCATGTTCTGGAGAAGAGCGAATTCAAGGACG AGTCTCAGTACTTCCGCTTCCATGCGGACGAGGAGATGGAAGGGACCAGCAGCAAGAACAAACAGCTTCG CAACGACTTCAAGCTAGTGGAGAACATCCTGGCCAAGCGGCTGCTGATCCCGCCGCAGGAGGATGACTAT GGCTTCGATCTTGAGGAGAAGAACAAGGCAGTGGTGGTGAAGTCCGTGCAGAGGGGTTCGCTGGCTGAGA TGGCTGGCCTGCAGGCGGGGAGGAAGATTTACTCCATCAACGAGGACCTGGTGTTCCTACGGCCCTTCTC TGAGGTGGAGACCATCCTTAACCAGTCCTTCTGCTCCCGCCGCCCGCTGCGCCTCCTGGTGGCCACCAAG GCCAAAGAGACCATCAAAATCCCTGACCACCCAGAGGCCCTGTCCTTCCAGATTCGAGGAACAGCCCCGC CGTGTGTCTTTGCCGTGGGCAGAGGTTCTGAGGCTGTGGCTGCCGGGCTCTGCGCAGGCCAATGCATCCT GAAAGTCAACGGCAACAGCGTGGCAAATGATGGCGCCCTTGAAGTTCTGGAGCACTTCCAGGCTTTCCGC AGCCGCAGGGAGGAGGCCCTGGGCCTTTACCAGTGGGTCTACCACAGCCACGAGGACGCCCAGCAGGCGA GAGCCAGCCAGGGTGCCCCGGATGAGGACCCCCAGGAGGATGACTCAGTCCTGCCCCTGCTGTCCCTGGG TCCCCAGCTGAGCCTGCACGAGGACAGCGCTGTGGTCAGCCTGACGCTGGACAATGTTCACTTGGAGCAC GGTGTGGTTTATGAATACGTGAGCACAGCAGGAGCCAAGTGTCACGTGCTGGAAAAGATCGTGGAGCCCC GAGGCTGTTTCCGCCTGGCTGCCAAGATCCTTGAGGCCTTTGCCGAGGACGACAGCCTCTTCGTGCAGAA CTGTGGGCGGCTCATGGCCATGGGCAGCGCTATCATCACCATGTCCCACTACGAGTTCCACAACATCTGT GACACCAAGCTGGAGAGCATTGGCCAGAGAATCGCCTGCTACCAGGAGTTTGCAGCCCAGCTGAAGAGCA GGGTCAGCCCCCCCTTCAAGCAGGCACCCCTGGAGGACCACCCGCTGTGTGGCCTGGACTTCTGCCCTAC CAACTGCCACATCAACTTCATGGAGGTGTCCTACCCCAAGACCACCCCTTCAGTGGGCAGGTCCTTCAGC ATCCGCTTTGGGCGCAAACCCTCCCTCATCGGCCTTGACCCAGAACAAGGCCACCTGAACCCCATGGCTT ACACCCAGCACTGTATCACCACCATGGCTGCCCCGTCCTGGAAGTGCGCGCCTGCTGTAGATGGGGACTC TCAAAGCCAAGGTCTCATTGACAGTATCTTCGTGTCAGCCAGTGGTGCCCTGAGCCAGCAAGAACGGGGC CTGAGCTTTCTGCTCAAGCAGGAGGACCGGGAGATCCAGGACGCGTATCTGCAGCTCTTCACTAAGCTGG ATGTGGCCCTCAAGGAGATGAAGCAGTATGTCACCCAGATCAACAGGCTGCTGTCAACCATCACAGAGCC CACGTCAGCTGCACCCTGTGACCCACCCTTGGTGGAGGAGACCTCATCTTCCCCACCGGCCAGTGAAGAG AGCGAAGTGGACAGGACTGACCATGGCGGCATCAAGAAGGTGTGCTTCAAGGTGTCAGAGGACGAGCAGG AAGACTCGGGCCACGACACCATGAGCTACCGAGACTCCTATAGTGAGTGTAACAGCAATCGTGACTCTGT CCTGTCCTACACCAGCGTGAGAAGCAACAGTTCGTACCTGGGCAGTGATGAGACAGGCTCAGGGGATGAG CTGCCCTGTGACATACGCATCCCATCCGACAAGCAGGACAAACTTCACGGCTGCCTGGAACATCTCTTTA ACCAGGTGGATTCCATCCATGCCCTGCTCAAGGGGCCTGTGATGAGCAGGGCGTTTGAGGAGACCAGGCA TTTCCCCATGGAGCACAGCTGGCAAGAGTTCAAACAGAAAGAAGAATGCACAGTCCGAGGCCGGAACTTG ATCCAGATCAGCATCCAGGAGGACCCTTGGAACCTACCCAGCTCCATCAGGACCCTGGTAGACAACATCC AGCGATATGTGGAAGATGGGAAGAACCAGCTGCTTCTGGCCCTGCTGAAATGCACAGACACGGAGCTGCA GCTGCGCAGGGATGCTGTCTTCTGTCAGGCCCTGGTGGCCGCCGTGTGCACCTTCTCGGAGCAGCTGCTG GCCGCCCTCGACTACCGCTACAACAACAACGGCGAGTATGAAGAGAGCAGCAGAGACGCCAGCCGCAAGT GGCTGGAGCAGGTGGCAGCCACCGGTGTCCTGCTGCACTGGCAGTCCCTGCTGGCACCAGCCACCGTGAA AGAGGAACGGACCATGCTGGAGGACATCTGGGTGACACTGTCGGAGCTGGACAATGTCACCTTCTCCTTT AAGCAGCTGGATGAGAATTCTGTGGCCAACACCAACGTCTTCTACCACATTGAGGGCAGCAGGCAGGCAC TGAAGGTAGTCTTCTACCTCGATGGCTTCCACTTCTCCAGGCTGCCCTCTCGCCTGGAGGGTGGGGCCAG CCTCAGGCTGCACACCGTGCTATTCACAAAAGCTCTGGAGAATGTGGAAGGGCCGTCGCCCCCAGGAAGC CAGGCTGCAGAAGATCTGCAGCAGGAGATCAATGCGCAGTCCCTGGAGAAGGTTCAGCAGTACTATAGAA GGCTCAGGGCCTTCTACCTGGAGCGGTCCAACCTCCCCACAGATGCTACCACCACAGCTGTGAAGATCGA CCAGCTGATCCGTCCCATCAACGCCCTGGACGAGCTCTACCGCCTCATGAAGTCCTTCGTGCACCCGAAA GCTGGTGCTGGTGGGAGCCTAGGTGCCGGCCTCGTCCCCGTCTCCTCTGAGCTCTGCTACCGTCTGGGGG CGTGTCAGATCACCATGTGTGGCACCGGCATGCAGCGGAGCACCCTGAGCGTGTCCTTGGAACAAGCAGC CATCTTGGCTCGGAGCCACGGGCTGCTGCCCAAGTGTGTTATGCAGGCCACAGACATCATGCGCAAGCAG GGCCCCCGAGTGGAGATCCTGGCAAAAAACCTCCGCATCAAAGACCCAATGCCCCAAGGTGCACCACGCC TCTACCGCCTCTGCCAGCCTCCGGTGGATGGAGACCTCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001135718
Insert Size	4941 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001135718.1, NP_001129190.1
RefSeq Size	5438 bp
RefSeq ORF	4941 bp
Locus ID	311647

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