Zfp423 (NM_053583) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Roaz; Znf423
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN211954 representing NM_053583 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGGCGGAAGCAGGCGAAGCCGCGATCGGTGAAAGTTGAAGAGGGGGAGGCCTCGGACTTCTCGC TGGCCTGGGATTCCTCTGTGGCAGCAGCGGGAGGCCTAGAAGGAGAGTCAGAGTGTGATCGGAAATCCAG CCGTGCGCTGGAAGACAGGAACAGCGTGACAAGTCAAGAGGAGAGAAACGAGGACGATGAAGATGTGGAA GATGAGTCAATTTACACCTGCGATCACTGTCAGCAGGACTTCGAGTCTCTGGCAGACCTGACGGACCACC GGGCCCACCGCTGTCCTGGAGATGGTGATGACGACCCACAGCTCTCCTGGGTGGCTTCATCTCCCTCCAG CAAGGATGTTGCGTCACCCACGCAGATGATCGGCGATGGTTGTGACCTTGGCCTCGGCGAGGAGGAAGGC GGCACCGGCCTGCCGTACCCTTGCCAGTTCTGCGACAAGTCCTTCATCCGCCTGAGCTACTTGAAGAGGC ATGAACAGATCCACAGCGACAAGCTGCCGTTCAAGTGCACCTTCTGCAGCCGCCTCTTCAAACACAAGAG GAGCCGGGACCGGCACATCAAGCTGCACACAGGCGACAAGAAGTACCACTGCCACGAGTGCGAGGCGGCT TTCTCCCGCAGGGACCACCTCAAGATCCACCTGAAGACCCACAGCTCCAGCAAGCCGTTCAAGTGCAGCG TGTGCAAACGCGGGTTCTCCTCCACCAGCTCCCTGCAGAGCCACATGCAGGCCCACAAGAAGAATAAGGA ACACCTGGCTAAGTCAGAGAAGGAAGCCAAGAAGGACGACTTCATGTGTGACTACTGCGAGGACACCTTT AGCCAGACAGAAGAGCTGGAGAAGCATGTGCTTACCCTCCACCCGCAGCTCTCAGAGAAGGCGGACCTCC AGTGTATCCACTGCCCCGAGGTCTTTGTCGACGAGAGCACGCTGCTGGCCCACATCCACCAAGCTCACGC CAACCAGAAACACAAGTGCCCCATGTGCCCTGAGCAGTTCTCCTCCGTGGAGGGTGTGTACTGCCACCTG GACAGCCACCGGCAGCCTGATTCCAGCAATCACAGTGTCAGCCCCGACCCCGTGCTGGGCAGTGTGGCTT CCATGAGCAGTGCTACACCTGACTCGACGCCCGACCCCGTGCTGGGCAGTGTGGCTTCCATGAGCAGTGC TACACCTGACTCGAGCGCCTCTGTGGAGCGCGGGTCCACGCCAGACTCCACCTTGAAGCCGCTGAGGGGG CAGAAGAAGATGCGGGATGACGGGCAGAGCTGGTCCAAGGTTGTCTACAGCTGCCCCTACTGTTCTAAGC GGGACTTTACCAGCCTGGCTGTGCTAGAGATTCATCTGAAGACCATTCACGCGGACAAACCTCAGCAGAG TCACACGTGTCAGATTTGCCTGGACTCCATGCCCACGCTCTACAACCTCAACGAGCATGTGCGCAAGCTG CACAAGAGCCACGCTTACCCCGTCATGCAATTCGGCAACATCTCCGCCTTCCACTGCAACTACTGCCCCG AGATGTTCGCGGACATCAACAGCCTGCAGGAGCACATCCGAGTCTCGCACTGTGGCCCCAATGCCAACCC CCCCGACGGGAACAATGCTTTCTTCTGTAACCAGTGTTCTATGGGCTTTCTCACTGAATCCTCCCTCACA GAGCACATCCAACAGGCACACTGCAGTGTGGGGAGCACCAAGCTGGAGTCTCCCGTTATCCAGCCCACAC AGTCCTTCATGGAGGTCTACTCCTGCCCTTACTGTACCAACTCCCCTATCTTTGGCTCCATCCTGAAGCT CACTAAGCACATCAAAGAGAACCACAAGAACATCCCGTTGGCACACAGCAAGAAGTCCAAGGCGGAGCAG AGTCCGGTCTCCTCTGACGTCGAGGTGTCTTCCCCGAAACGACAGCGCCTCTCGGGGAGTGCCAACTCCA TCTCTAACGGCGAGTACCCCTGCAATCAGTGCGACCTCAAGTTCTCCAACTTCGAGAGCTTCCAGACCCA CTTGAAGCTGCACCTGGAGCTGCTGCTCCGGAAGCAGGCCTGCCCCCAGTGCAAAGAGGACTTCGACTCT CAGGAGTCCCTCCTGCAGCATCTGACCGTGCACTACATGACCACGTCCACCCACTACGTCTGCGAGAGCT GTGACAAGCAGTTCTCCTCAGTGGACGACCTGCAGAAGCACCTGCTGGACATGCACACCTTTGTGCTATA CCACTGCACCCTGTGTCAGGAGGTCTTCGACTCTAAGGTGTCCATCCAGGTGCACCTGGCCGTGAAGCAC AGCAACGAGAAGAAGATGTACCGTTGCACCGCCTGCAACTGGGACTTCCGCAAGGAGGCTGACCTGCAGG TGCACGTCAAGCACAGTCACCTCGGCAACCCGGCCAAGGCCCACAAGTGCATCTTCTGTGGTGAGACCTT CAGCACCGAGGTGGAGCTCCAGTGCCACATCACCACGCACAGCAAGAAGTACAATTGCAGGTTCTGCAGC AAAGCCTTCCACGCCGTCCTCCTGCTGGAGAAGCACCTTCGGGAGAAGCATTGTGTGTTTGACCCAGCTG CAGAGAATGGCACGGCCAACGGGGTGCCCCCCACCTCCACCAAGAAGGCAGAGCCCGCCGACCTGCAGGG CATGCTGCTCAAGAACCCTGAGGCACCGAACAGCCACGAGGCCAGTGAGGACGATGTGGATGCATCAGAG CCCATGTACGGCTGTGACATCTGTGGTGCAGCCTACACCATGGAGGTGCTGCTGCAGAACCACCGACTCC GGGATCATAACATCCGGCCCGGAGAGGACGATGGCTCACGCAAGAAGGCAGAGTTCATAAAGGGCAGCCA CAAGTGTAACGTGTGCTCTCGGACTTTCTTCTCGGAGAACGGGCTCCGGGAACACCTGCAGACGCACCGG GGCCCTGCCAAGCACTACATGTGTCCCATCTGTGGCGAGCGCTTCCCCTCGCTGCTGACGCTCACTGAGC ACAAGGTGACCCACAGCAAGAGTCTGGACACAGGCACCTGTCGCATCTGCAAAATGCCCCTGCAGAGTGA GGAGGAGTTTATCGAGCACTGCCAGATGCACCCCGACTTGCGGAACTCCCTCACTGGTTTCCGCTGTGTG GTCTGTATGCAGACTGTCACCTCAACCCTGGAGCTCAAGATCCATGGCACCTTTCACATGCAGAAGCTGG CTGGCAGCTCAGCTGCTTCCTCCCCCAATGGCCAGGGGCTGCAGAAGCTCTACAAGTGCGCCCTGTGCCT CAAAGAGTTCCGTAGCAAGCAGGACCTGGTCAGGCTTGACGTCAATGGGCTGCCCTATGGCCTATGTGCC GGCTGCATGGCCCGTAGTGCCAATGGACAGGTGGGTGGCCTGGCCCCACCCGAACCTGCCGACCGGCCCT GCGCTGGCCTCCGCTGCCCTGAATGTAACGTGAAGTTTGAGAGTGCTGAGGACCTGGAGAGCCACATGCA GGTGGACCACCGTGATCTTACCCCAGAGACCAGTGGGCCCCGGAAAGGTGCCCAGACGTCACCAGTGCCC CGGAAGAAGACGTACCAGTGCATCAAGTGCCAGATGACCTTCGAGAACGAGAGAGAGATCCAGATCCACG TCGCCAACCACATGATCGAGGAAGGCATCAACCATGAGTGTAAGCTGTGCAACCAGATGTTCGACTCCCC AGCCAAGCTCCTTTGTCACCTCATCGAACACAGCTTCGAGGGCATGGGTGGTACTTTCAAGTGCCCCGTG TGCTTCACAGTCTTCGTCCAGGCCAACAAGCTGCAACAGCACATCTTCGCCGTGCACGGGCAGGAGGACA AAATCTACGACTGCTCGCAGTGCCCACAGAAGTTTTTCTTCCAGACAGAGTTGCAGAACCACACGATGAG CCAGCACGCACAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053583
Insert Size	3936 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053583.2, NP_446035.2
RefSeq Size	4667 bp
RefSeq ORF	3936 bp
Locus ID	94188
UniProt ID	O08961
Gene Summary	zinc finger protein that interacts with the Olf-1/EBF transcription factor; plays a role in regulation of differentiation of olfactory neurons [RGD, Feb 2006]

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