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Mthfd1 (NM_022508) Rat Untagged Clone
CAT＃: RN212063
Mthfd1 (untagged ORF) - Rat methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (Mthfd1), (10 ug)
CNY 12,070.00
Product images
Specifications
| Product Data | |
| Type | Rat Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RN212063 representing NM_022508
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCAGCGGGAATCCTGAACGGGAAAGTGGTCTCCGCGCAAATCAGGAATCGACTGAAGACCCAGG TTACTCAGATGCAGGAGCAGGTACCTGGCTTCACCCCTGGCCTGGCAATCCTGCAGGTTGGCGACAGAGA TGATTCCAATCTTTATATAAATGTGAAGCTGAAGGCTGCTCAAGAGATCGGGATCAAAGCCACTCACATT AAATTACCAAGAACTTCCACAGAGTCGGAGGTGTTAAAATACGTCATCTCCCTGAATGAGGATGCCACTG TGCACGGATTCATAGTACAGCTGCCTTTAGACTCCGAGAATTCCATTAACACAGAGGCAGTCATCAATGC CATTGCCCCTGAGAAGGATGTGGACGGGTTGACGAGCATCAATGCCGGGAAGCTTGCCAGAGGTGATCTA AAGGACTGCTTCATTCCGTGCACACCCAAAGGATGCTTGGAACTCATCAAAGAGACAGGAGTGCAGATTG CAGGAAGGCACGCCGTGGTGGTCGGGCGAAGTAAAATAGTTGGTGCACCCATGCACGACCTGCTTCTGTG GAACAATGCCACGGTGACCACCTGCCACTCCAAGACTGCCGATCTGGACAAGGAGGTAAATAAAGGAGAC ATTCTGGTGGTTGCAACAGGACAGCCTGAGATGGTGAAAGGGGAGTGGATCAAGCCTGGAGCTGTGGTCA TAGACTGTGGGATCAATTATGTTCCAGATGATACAAAACCAAATGGAAGGAAAGTTGTGGGTGACGTAGC ATATGACGAGGCCAAGGAGAAGGCGAGCTTTATCACGCCTGTCCCCGGTGGCGTGGGGCCCATGACCGTG GCAATGCTGATGCAGAGCACAGTAGAGAGCGCACAGCGCTTCCTGAAGAAATTTAAGCCAGGGAAGTGGA CAATTCAGTATAACAAGCTGAACCTCAAGACGCCTGTACCAAGTGACATTGCTATATCACGATCTTGCAA ACCCAAGCTCATCGGTAACCTGGCCCGAGAAATTGGGCTACTCACTGAGGAGGTGGAATTGTATGGAGAA ACAAAGGCCAAGGTCTTACTGTCAGCACTAGATCGCCTGAAGCATCAGCCAGATGGGAAATACGTTGTGG TGACTGGAATTACTCCAACACCCCTGGGAGAAGGGAAGAGCACAACCACCATCGGGCTTGTGCAAGCCCT TGGCGCCCACCTGCATCAGAATGTTTTTGCGTGTGTGCGACAGCCTTCTCAGGGCCCCACCTTTGGGATA AAAGGTGGCGCTGCAGGAGGCGGCTATTCCCAGGTCATTCCTATGGAAGAGTTTAATCTCCACCTCACTG GTGACATCCACGCCATCACTGCCGCTAATAACCTTGTGGCTGCTGCTATTGACGCTCGGATATTTCATGA GCTGACCCAGACAGACAAGGCTCTCTTTAATCGTCTGGTACCCTCAGTAAATGGAGTAAGAAAGTTCTCT GACATCCAAATCCGCAGGTTACGGAGGCTAGGCATTGAGAAGACTGACCCCGCTGCGCTGACAGATGATG AGATAAACAGATTTGCAAGACTAGATATTGATCCAGAAACCATAACATGGCAGAGAGTGCTGGATACTAA CGATAGATTCCTGAGGAAGATCACCATTGGACAGGCTCCAACCGAGAAAGGCCACACACGCACGGCCCAG TTTGATATCTCTGTGGCCAGTGAAATCATGGCAGTCCTGGCCCTCACTAGTTCTCTGGAAGACATGAGAG CAAGGCTGGGCAAAATGGTGGTAGCGTCCAGTAAGAAAGGGGAGCCTATCAGCTGTGAAGACCTGGGCGT GAGCGGGGCGCTGACGGTGCTCATGAAGGATGCGATCAAGCCCAATCTCATGCAGACCTTAGAGGGCACG CCGGTATTTGTCCATGCTGGGCCTTTTGCCAACATTGCACATGGGAATTCCTCCATCATTGCAGACCGGA TTGCACTCAAGCTGGTTGGCCCTGAGGGCTTTGTAGTGACAGAAGCAGGATTCGGAGCAGACATAGGAAT GGAAAAGTTCTTCAACATCAAGTGCCGGTATTCTGGTCTCCAGCCTCATGTGGTGGTTCTTGTTGCCACT GTCAGGGCTCTTAAGATGCACGGGGGTGGCCCCACGGTCACCGCTGGACTGCCTCTTCCCAAGGCTTACA CAGAAGAGGACCTGGACCTGGTGGAAAAGGGCTTCAGTAACTTGAGGAAACAGATAGAAAATGCTAGAAT GTTTGGAGTGCCTGTCGTGGTGGCCATGAATGCATTCAAGACAGATACAGATACTGAGCTGGACCTCATC GGCCGCCTCTCCAGAGAGCATGGGGCTTTCGATGCTGTCAAGTGCACCCACTGGGCAGAAGGGGGCCAGG GAGCCTTAGCCCTGGCTCAGGCTGTCCAGAGAGCTTCACAGGCCCCCAGCAGCTTCCAGCTCCTCTATGA CCTCAAGCTCTCAGTTGAAGATAAAATCAGGATTATTGCACAGAAGATCTACGGGGCCGATGACATCGAA TTGCTCCCTGAAGCACAAAACAAAGCAGAAATCTACACAAAGCAGGGCTTTGGGAATCTACCCATCTGCA TGGCCAAAACACACTTGTCTTTATCTCACAACCCAGAGCAAAAAGGGGTGCCTACTGGCTTCGTTCTGCC CATCCGGGACATCCGGGCCAGCGTTGGGGCTGGTTTCCTGTACCCTTTAGTAGGAACGATGAGCACAATG CCTGGACTCCCTACTCGACCCTGTTTTTATGATATCGATTTGGACCCTGAAACTGAACAAGTGAATGGAT TGTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_022508 |
| Insert Size | 2808 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022508.1, NP_071953.1 |
| RefSeq Size | 3332 bp |
| RefSeq ORF | 2808 bp |
| Locus ID | 64300 |
| UniProt ID | P27653 |
| Gene Summary | a trifunctional enzyme combining a 10-formyl-H4folate synthetase, a 5,10-methenyl-H4folate cyclohydrolase, and a 5,10-methylene-H4folate dehydrogenase [RGD, Feb 2006] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RR212063 | Mthfd1 (Myc-DDK-tagged ORF) - Rat methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (Mthfd1), (10 ug) |
CNY 12,070.00 |
|
| RR212063L3 | Lenti ORF clone of Mthfd1 (Myc-DDK-tagged ORF) - Rat methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (Mthfd1), (10 ug) |
CNY 13,680.00 |
|
| RR212063L4 | Lenti ORF clone of Mthfd1 (mGFP-tagged ORF) - Rat methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (Mthfd1), (10 ug) |
CNY 15,110.00 |
