Myo5a (NM_022178) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	D; Dop; Myh12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212159 representing NM_022178 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCGTCCGAGCTCTACACCAAGTTTGCCAGGGTTTGGATCCCTGATCCTGAGGAAGTGTGGAAAT CGGCAGAGTTGCTCAAGGATTATAAGCCTGGAGATAAAGTGCTCCTGCTTCACCTTGAGGAAGGGAAGGA TTTGGAATACCGTCTAGATCCAAAGACCAGCGAGCTCCCTCACCTACGGAACCCTGACATACTTGTTGGA GAAAATGACCTCACAGCCCTCAGCTACCTTCACGAGCCTGCTGTGCTGCACAATCTCCGCGTCCGCTTCA TCGACTCCAAACTCATTTATACGTATTGTGGGATAGTCCTGGTAGCTATAAATCCCTATGAGCAGCTGCC TATCTATGGAGAAGATATCATTAATGCATACAGTGGCCAGAACATGGGTGACATGGACCCTCACATCTTT GCAGTAGCTGAAGAGGCCTATAAACAAATGGCAAGGGACGAACGAAATCAGTCCATCATTGTAAGTGGAG AGTCAGGTGCAGGGAAGACAGTCTCGGCTAAGTATGCCATGAGGTACTTCGCAACTGTAAGTGGCTCTGC CAGTGAGGCCAATGTTGAGGAAAAGGTCTTGGCCTCCAACCCCATCATGGAGTCAATTGGAAATGCTAAA ACAACCAGGAATGATAATAGCAGCCGGTTTGGGAAATATATTGAAATTGGTTTTGACAAGAGATATCGAA TCATCGGTGCCAATATGAGAACTTACCTTTTAGAGAAATCCAGAGTGGTGTTCCAGGCAGAAGAGGAGAG AAACTACCATATCTTCTATCAGCTCTGTGCTTCGGCAAAGCTACCTGAGTTTAAGATGCTGCGGTTAGGA AATGCAGATAGTTTCCATTACACGAAGCAAGGAGGCAGCCCTATGATAGAAGGAGTGGACGATGCGAAGG AGATGGCACACACCAGGCAGGCCTGCACTCTGCTGGGAATTAGTGAATCTTACCAAATGGGAATTTTTCG GATACTTGCTGGCATTCTTCACTTAGGCAATGTTGGGTTTGCGTCTCGAGATTCAGACAGCTGCACAATA CCTCCCAAGCACGAGCCTCTTATCATTTTCTGTGATCTTATGGGTGTGGATTATGAAGAGATGTGTCACT GGCTCTGCCACCGAAAGCTAGCTACCGCCACAGAGACGTACATCAAGCCCATCTCCAAGCTGCAGGCCAC AAATGCCCGAGACGCTTTAGCAAAGCATATCTATGCAAAGCTCTTTAACTGGATTGTCGGCCATGTCAAT CAGGCTCTCCATTCAGCTGTCAAGCAGCACTCTTTCATCGGCGTGCTGGACATTTATGGATTTGAAACAT TTGAGATAAATAGTTTTGAACAGTTCTGCATAAATTATGCAAATGAAAAGCTACAGCAACAATTCAACAT GCATGTCTTCAAACTGGAACAGGAGGAGTACATGAAGGAACAAATTCCATGGACACTCATAGATTTCTAT GATAATCAGCCTTGTATCAATCTTATAGAATCTAAACTGGGTATTCTAGATTTGCTGGATGAGGAATGTA AGATGCCTAAAGGTACCGATGACACATGGGCCCAAAAACTATACAACACACATTTGAACAAATGTGCTCT CTTTGAGAAGCCCCGCATGTCAAACAAAGCTTTCATCATCAAACATTTTGCTGACAAAGTGGAGTACCAG TGTGAAGGTTTTCTTGAAAAGAATAAAGACACTGTTTTTGAAGAACAAATTAAAGTCCTTAAGTCAAGCA AGTTTAAGATGCTACCAGAACTATTTCAAGATGACGAGAAGGCCATCAGTCCCACCTCTGCCACTTCCTC AGGACGCACACCTCTCACACGGGTACCTGTAAAGCCCACCAAGGGTCGACCTGGCCAGACTGCCAAAGAG CACAAGAAGACAGTGGGACTTCAGTTTCGAAACTCCCTTCACCTGCTTATGGAAACCCTTAACGCCACTA CTCCTCACTATGTTCGCTGTATTAAGCCCAATGATTTCAAGTTTCCATTCACATTTGATGAGAAGAGAGC AGTACAGCAGCTAAGAGCATGTGGTGTCCTGGAGACCATCCGGATCAGCGCAGCAGGGTTTCCCTCAAGG TGGACTTATCAAGAGTTTTTCAGCCGGTACCGGGTCCTAATGAAGCAAAAGGATGTGCTGGGAGATAGAA AGCAAACATGCCAGAATGTATTAGAGAAACTGATACTGGACAAGGATAAATACCAGTTTGGTAAGACAAA GATCTTTTTTCGTGCTGGTCAAGTGGCCTATCTAGAAAAATTGAGGGCTGACAAACTGAGGGCGGCCTGC ATCCGGATCCAGAAGACGATTCGTGGGTGGCTGCTGAGGAAGAGATACCTGTGTATGCAGAGGGCAGCCA TCACAGTGCAGCGATACGTGCGTGGCTATCAGGCTCGATGCTATGCTAAGTTTCTGCGCAGAACCAAGGC AGCAACCACCATTCAGAAGTACTGGCGCATGTATGTGGTCCGCAGGAAGTACAAGATTAGACGAGCTGCC ACCATTGTTCTTCAGTCTTATTTGAGAGGCTACTTGGCAAGAAATAGGTATCGCAAGATACTCCGTGAGC ACAAAGCAGTCATCATTCAGAAACGTGTCCGTGGCTGGCTGGCTCGTACACATTATAAGAGGACCATGAA AGCCATCATCTACCTTCAATGCTGCTTCCGGCGGATGATGGCCAAGCGTGAGCTGAAGAAACTCAAAATT GAGGCTCGCTCTGTGGAACGCTACAAGAAGCTCCATATTGGCATGGAAAACAAGATTATGCAGCTGCAAC GCAAAGTGGATGAGCAGAATAAAGACTACAAATGCCTCATGGAGAAACTGACCAATCTAGAAGGAGTATA CAACTCTGAGACTGAAAAACTACGAAATGATGTAGAACGTCTTCAGCTAAGCGAGGAGGAAGCTAAGGTT GCCACTGGGAGAGTGCTTAGTCTGCAGGAAGAAATTGCAAAACTCCGAAAAGACCTGGAGCAAACTCGAT CGGAGAAAAAGTCCATTGAAGAACGAGCAGATAAATACAAACAAGAAACTGAGCAGCTGGTGTCAAACTT GAAGGAAGAAAACACTCTGCTGAAGCAGGAAAAGGAGACCCTCAATCACCTCATGGTGGAGCAGGCTAAG GAGATGACAGAAACTATGGAGAGGAAGTTAGTAGAAGAAACAAAACAACTGGAGCTCGACCTGAATGATG AGAGGCTGAGGTATCAAAACCTCCTGAATGAGTTCAGTCGTCTGGAGGAGCGCTATGACGACCTCAAGGA AGAGATGACCCTGATGCTGAATGTGCCTAAGCCAGGACACAAGAGAACAGACTCTACCCACAGCAGCAAT GAGTCTGAATACACCTTCAGCTCAGAATTTGCAGAAACTGAAGACATTGCACCAAGGACAGAGGAGCCAA CTGAGAAGAAGGTGCCTTTGGATATGTCATTGTTCCTTAAGCTCCAGAAGCGTGTTACAGAGCTGGGACA GGAGAAGCAGTTGATGCAGGATGAGCTGGACCGAAAGGAGGAGCAGGTGTTGCGCAGCAAGGCAAAGGGA GGAGAAAGACCACAGATTAGAGGTGCTGAACTGGGATATGAGTCCCTCAAGCGTCAGGAACTGGAGTCAG AAAACAAAAAGCTGAAGAATGAGCTGAATGAGCTACGCAAAGCCCTGAGTGAGAAGAGTGCCCCCGAAGT GAATGCGCCAGGCGCGCCCGCTTACCGCGTCCTCATGGAGCAGCTGACGGCCGTGAGCGAGGAGCTCGAT GTCCGCAAGGAGGAAGTCCTCATTTTAAGGTCTCAGCTGGTGAGCCAGAAAGAAGCCATCCAACCCAAGG ATGACAAGAATACAATGACAGATTCCACAATACTTTTGGAAGATGTACAGAAAATGAAAGACAAAGGTGA AATAGCACAAGCATATATTGGTTTGAAAGAAACGAACAGGCTCTTAGAATCCCAGCTACAGTCGCAGAAG AGGAGCCATGAAAATGAGGCTGAGGCCCTCCGTGGGGAGATCCAGAGCCTAAAGGAAGAAAACAACCGGC AACAGCAGCTGCTGGCCCAGAACCTGCAGCTGCCCCCTGAGGCCCGCATTGAGGCCAGCCTGCAGCATGA GATCACCCGGCTGACCAATGAAAACCTGGATCTGATGGAACAACTTGAAAAGCAGGATAAAACCGTCCGG AAACTGAAGAAACAACTAAAAGTCTTTGCCAAAAAAATCGGTGAACTAGAAGTGGGGCAGATGGAGAACA TATCCCCAGGACAGATCATCGATGAGCCTATCCGGCCGGTCAACATTCCCCGGAAAGGAAAGGATTTCCA AGGGATGCTGGAGTACAAGCGGGAGGACGAACAGAAGCTTGTTAAGAACCTGATTCTAGAACTAAAGCCA CGTGGTGTGGCTGTCAATCTGATTTCAGGGTTACCGGCATATATCTTGTTTATGTGTGTGCGACATGCCG ACTACCTGGACGATGATCAGAAAGTAAGATCATTGCTGACATCAACAATTAACAGCATCAAAAAAGTCCT GAAGAAAAGAGGTGACGATTTTGAAACTGTCTCCTTCTGGCTCTCTAACACATGTCGATTTTTGCACTGT TTGAAGCAATATAGTGGAGAAGAGGGCTTCATGAAACACAACACGTCTCGCCAGAATGAACACTGCCTCA CCAATTTTGACCTTGCTGAGTATCGGCAAGTACTGAGTGACTTGGCCATTCAGATCTATCAGCAGCTTGT GAGGGTGTTAGAGAACATTCTTCAGCCAATGATAGTCTCAGGCATGCTAGAGCATGAAACAATTCAGGGA GTATCTGGGGTGAAGCCCACAGGACTCAGAAAGCGAACCTCCAGTATCGCCGATGAGGGCACCTACACAC TGGACTCCATCCTGCGGCAGCTCAACTCCTTCCATTCCGTCATGTGTCAGCATGGCATGGACCCAGAGCT AATCAAGCAGGTGGTCAAGCAGATGTTCTACATCGTGGGCGCCATCACCCTGAACAACCTCCTGCTGCGC AAGGACATGTGCTCCTGGAGCAAAGGCATGCAGATCAGGTACAATGTCAGTCAATTGGAAGAATGGCTAC GTGACAAGAATCTAATGAACAGTGGGGCAAAAGAGACTCTGGAACCTCTTATCCAGGCTGCTCAGCTTTT GCAAGTGAAAAAGAAAACTGACGATGACGCGGAAGCCATCTGCTCCATGTGCAACGCTCTGACCACAGCC CAGATTGTCAAAGTGTTGAATCTGTACACACCCGTTAATGAGTTTGAGGAAAGGGTCTCTGTTTCATTTA TCCGCACTATACAGGTGCGGTTACGAGACAGGAAAGACTCTCCACAGCTGCTCATGGATGCAAAACACAT CTTTCCTGTCACTTTCCCCTTTAACCCATCCTCCCTGGCCCTAGAAACCATCCAGATTCCAGCCAGCCTG GGCCTGGGCTTCATTGCAAGGGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_022178
Insert Size	5487 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_022178.1, NP_071514.1
RefSeq Size	5620 bp
RefSeq ORF	5487 bp
Locus ID	25017
UniProt ID	Q9QYF3
Gene Summary	may play a role in secretory granule transport [RGD, Feb 2006]

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