Ift172 (NM_053792) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Slb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212242 representing NM_053792 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTTGAAGCACCTGAGGACCCTGCTGAGCCCTCAGGATGGAGCTGCGAAGGTGACCTGCATGGCCT GGTCCCAGAACAATGCTAAATTTGCTGTCTGCACAGTGGACCGAGTGGTACTGCTGTACGATGAACACGG GGAGCGGAGAGACAAGTTCTCCACCAAACCAGCTGACATGAAGTATGGCAGGAAGAGCTACATGGTCAAA GGCATGGCTTTCTCTCCTGATTCCACAAAAATTGCCATAGGACAGACTGACAACATCATCTATGTCTACA AGATAGGAGAAGACTGGGGTGACAAGAAGGTCATCTGCAACAAGTTCATCCAGACGAGTGCCGTCACTTG TCTGCAGTGGCCTGCGGAGTATATCATTGTCTTTGGACTGGCTGAAGGCAAGGTTCGCTTAGCAAACACA AAAACTAACAAGTCATCTACTATCTATGGGACAGATTCCTACGTGGTAGCGCTGACAACCAACTGCTCTG GGAAAGGGATTCTCTCGGGTCACGCGGACGGCACCATCGTTAGGTATTTCTTCGATGACGAAGGCTCGGG AGAGTCACAGGGGAAGTTGGTTAACCATCCATGCCCACCCTATGCCTTGGCCTGGGCCACCAACAGCATT GTGGCTGCTGGCTGTGATCGGAGGATTGTGGCCTATGGAAAGGAAGGCCATGTGCTACAGACTTTCGACT ATAGCCGTGACCCTCAGGAGCGGGAGTTCACCACAGCTGCTGCAAGTCCTGGAGGCCAGTCTGTTGTTCT TGGAAGTTATGACAGACTTCGGGTGTTCAACTGGAGTCCTCGAAGAAGCATCTGGGAAGAGGCAAAGCCC AAGGAGATTGCCAACTTATACACAGTCACCGCCTTGGCCTGGAAGCGGGATGGCTCTCGGCTCTGTGCGG GAACACTCTGTGGTGGGGTGGAACAGTTTGACTGCTGCCTCCGAAGGAGCATTTACAAGAACAAGTTCGA GCTGACATATGTGGGCCCTAGCCAGCTGATTGTGAAGAACCTGTCTTCGGGGACCAGGGTGGTGCTCAAG TCACACTATGGCTACGAAGTGGAAGAGGTGAAAATCCTGGGAAAGGAACGTTACCTGGTGGCTCACACTT CAGACACGCTGCTGCTGGGAGACCTCAACACAAACCGCCTCAGTGAGATACCCTGGCAGGGATCGGGTGG CAATGAGAAATATTTCTTTGAAAATGAGAATGTCTGCATGATCTTCAATGCTGGAGAGCTGACCCTGGTG GAATATGGGAGTAACGACTCCTTGGGTTCTGTGCGCACTGAATTCATGAACCCTCACCTCATCAGCGTCC GTATCAATGAGAGGTGCCAGCGAGGAATGGAAGACAATAAGAAATTGGCTTATCTTGTTGATATTAAGAC GATTGCTATAGTGGATCTGATCGGTGGCTACAACATTGGTACTATCAGTCACGAAAGCCGTGTGGATTGG CTGGAACTTAACGAAACTGGGCACAAGCTTCTCTTCAGAGACCGGAAACTTCGTTTGCATCTGTACGATA TTGAAAGCTGCTCTAAGACAATGATCCTGAACTTCTGCTCCTACGTGCAGTGGGTTCCCGGGAGTGATGT GCTGGTGGCTCAGAACCGGAACAGCTTATGTGTCTGGTACAATATCGAGGCACCTGAGAGAGTCACCATG TCCTCTATCAGGGGTGATGTTGTCGGTCTGGAGAGAGGTGGTGGGAAGACGGAGGTCATGGTGACAGAAG GTGTGACTACTGTGGCCTACACCCTGGATGAGGGCCTGATTGAGTTTGGAACAGCCATCGATGACGGCAA CTACACCCGGGCAACAGCCTTCTTGGAGACCCTGGAGATGACCCCAGAAACAGAGGCAATGTGGAAAACC TTGAGCAAACTGGCCCTGGAAGCAAGGCAGCTTCACACTGCTGAGAGGTGCTTCTCTGCTTTGGGTCACG TGGCCAAAGCTCGGTTCCTGCATGAGACCAATGAGATAGCGGATCAGGTGTCCAGGGAATATGGTGGAGA AGGAACGGACTTCTATCAGGTTCGAGCTCGCCTCGCCATGCTGGAGAAGAACTACAAACTGGCCGAAATG ATCTTCTTGGAACAGAATGCTGTTGAAGAAGCCATGGACATGTACCAGGAGCTGCACCGCTGGGATGAAT GCATTGCTGTGGCTGAGGCCAAGGGACACCCAGCCCTGGAGAAGCTACGCAGAGATTACTATCAGAGATT AATGGACACACAGCAAGAGGAACGAGCAGGTGAACTGCAGGAGAGTCAAGGGGATGGGCTGGCAGCCATC AGCCTCTACCTCAAAGCTGGGCTCCCTGCCAAAGCCGCACGGCTGGTGCTGACGCGAGAGGAGCTGCTGG CCAACACGGAGCTGGTAGAACATATCACCACAGCCCTCATCAAGGGAGAGCTCTATGAAAGGGCAGGTGA TCTCTTTGAGAAGATTCGAAATCCACAGCGGGCCCTTGAGTGCTACTGTAAAGGCAACGCATTCATGAAA GCTGTTGAGCTGGCTCGACTGGCCTTCCCAGTGGAGGTGGTGAGGCTGGAGGAGGCCTGGGGGGACCACT TGGTACAGCAAAAGCAGCTGGACGCGGCCATCAATCACTATATTGAAGCCAGGTGCTCCATTAAAGCAAT TGAGGCTGCCCTGGGTGCCCGCCAGTGGAAGAAGGCAATTTATATATTAGATCTGCAGGATAGAAACACT GCATCTAAATACTATCCTCGTGTGGCCCAACACTATGCATCTCTGCAGGAGTATGAGATCGCTGAGGAGC TCTACACCAAAGGAGACCGGACCAAAGATGCTATAGACATGTACACCCAGGCTGGTCGCTGGGAGCAAGC CCACAAGCTGGCCATGAAGTGCATGCGACCAGAAGACGTGTCAGTGCTCTACATCACACAGGCCCAGGAA ATGGAGAAGCAGGGCAAGTACCGAGAGGCCGAGAGGCTGTACGTGACAGTGGAGGAGCCTGATCTTGCCA TCACCATGTTCAAAAAGCACAAGTTGTATGACGACATGATTCGCCTGGTGGGGAAGCACCATCCAGACCT CCTCAGCGACACACACCTTCACCTGGGCAAGGAGCTGGAGACAGAAGGCCGACTGCAAGAGGCTGAGTAC CACTACCTCGAGGCCCAGGAATGGAAGGCAACTGTGAACATGTACCGGTCCAGCGGGCTTTGGGAGGAGG CCTACCGGGTGGCCAAAGCCCACGGAGGAGCCAACGCCCACAAACACGTGGCCTATCTGTGGGCGAAGAG CCTGGGAGGAGAGGCTGCGGTCAGACTGCTCAACAAGCTGGGGCTTCTAGAGGCTGCCATTGACCATGCT GCTGACAACTGCTCCTTTGAGTTTGCCTTTGAACTCTCTCGGCTAGCCCTCAAGCACAAAACCCCGGAGA TTCATCTCAGATACGCCATGTACCTGGAAGATGAGGGTAAATTTGAAGAGGCTGAAGCTGAATTCATCCG GGCTGGTAAACCCAAGGAGGCAGTCCTCATGTTTGTCCACAACCAGGATTGGGAGGCAGCTCAGCGTGTG GCTGAGGCCCACGATCCTGACAGTGTTGCTGAGGTACTTGTAGGCCAGGCCCGTGGGGCCTTGGAGGAGA AGGACTTTCAGAAGGCAGAAGGGTTGCTGCTTCGGGCCCAGAGACCAGGCCTGGCTCTCAATTATTATAA GGAGGCTGGACTCTGGAGTGATGCTCTGAGGATCTGCAAGGACTATGTGCCTGGCCAGCTGGAGGCCCTG CAGGAAGAGTATGAGCGAGAAGCTACCAAGAAGGGGGGCAGAGGCGTGGAGGGACTTGTAGAACAGGCAC GGCAATGGGAACAGGCTGGAGAGTATAGCCGTGCAGTGGACTGCTACCTCAAAGTTCGAGACTCAGGAAG CAGTGGCCTGATGGAGAAGTGCTGGATGAAGGCGGCTGAACTGTCCATCAAGTTCCTGCCTCCCCAGCGA AGTCTGGAAGTGGTTCGGGTTGTAGGTCCCCAGCTGATTGGAATTGGAAAGCACAGTGCTTCTGCAGAAC TCTATCTGAACCTGGACCTTGTCAAAGAAGCAATTGATGCCTTCATTGAGGGTGAAGAGTGGAACAAGGC CAAGCGCGTGGCCAAAGAGCTAGATCCCCGGTATGAAGACTATGTGGATCAGCATTATAAGGAGTTCCTC AAGAACCAGGGTAAAGTGGATTCGCTGGTGGGTGTGGACGTAGTAGCTGCCTTGGACCTGTATGTGGAGC AGGGCCAGTGGGATAAGTGCATTGAAACGGCTACCAAACAGAACTACAAGATTCTGCACAAGTATGTGGC TCTGTATGCGACTCATCTGATCCGAGAAGGTGGCTATGCCCAGGCCCTGGCCCTCTACGTGCAACATGGA GCCCCTGCTAACCCCCAGAATTTCAACATCTACAAAAGGATCTTCACTGACATGGTTAGCTCTCCTGGGA CCAACAATGCTGAGGCCTACCATAGCTGGGCTGACCTTCGGGATGTCCTCTTCAACCTGTGTGAAAACCT GGTGAAGTCCAGCGAGGCAAACTCGGCAGCTCATGAGGAGTTTGAGATGATGCTGCTGATCGCTCATTAC TATGCGACACGATCCGCAGCGCAGAGCATCAAACAGCTGGAAACCGTAGCTGCTAGACTTTCCGTTTCGC TCTTGCGCCACACCCAGTTACTACCTGCGGACAAGGCCTTTTATGAAGCAGGCACTGCTGCCAAGGAAGT TGGCTGGGAGAACATGGCCTTCATTTTCTTCAACCGATTTTTGGATCTGACTGATGCCATCGAAGAAGGG ACCTTGGATGCCCTGGACCACTCTGATTTTCAGGACACAGACATTCCCTTTGAGGTGCCACTCCCTGCCA AGCAGCATGTCCCGGAGGCCCAGAGAGAAGAGGTTCGAGACTGGGTGCTCACAGTCTCGATGGACCAGAG GCTGGAGCAGGTTCTGCCCCGAGATGAGCGTGGTGTCTACGAGGCTTCCCTAGTGGCAGCGAGCACTGGA GTCCGGGCCCTGCCCTGTCTCATTACAGGTTACCCCATTCTGAGGAACAAAATTGAATTTAAGCGGCCTG GGAAGGCTGCTAACAAAGACAACTGGAACAAGTTTCTTATGGCCATCAAGACCTCCCACAGTCCAGTGTG CCAGGATGTGCTCAAATTCATCAGCCAGTGGTGTGGTGGGCTCCCCAGCACCAGCTTTTCCTTTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053792
Insert Size	5250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053792.1, NP_446244.1
RefSeq Size	5392 bp
RefSeq ORF	5250 bp
Locus ID	116475
UniProt ID	Q9JKU3
Gene Summary	selectively interacts with Lhx3 and Lhx4; may be involved in modulating the transcriptional activity of specific LIM factors [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.