Col1a1 (NM_053304) Rat Untagged Clone

CAT#: RN212285

Col1a1 (untagged ORF) - Rat collagen, type I, alpha 1 (Col1a1), (10 ug)



  "NM_053304" in other vectors (1)

CNY 23,370.00


货期*
14周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms COLIA1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN212285 representing NM_053304
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCAGCTTTGTGGACCTCCGGCTCCTGCTCCTCTTAGGGGCCACTGCCCTCCTGACGCATGGCCAAG
AAGACATCCCTGAAGTCAGCTGCATACACAATGGCCTAAGGGTCCCTAATGGTGAGACGTGGAAACCTGA
TGTATGCTTGATCTGTATCTGCCACAATGGCACGGCTGTGTGCGATGGCGTGCTATGCAAAGAAGACTTG
GACTGTCCCAACCCCCAAAAACGGGAGGGCGAGTGCTGTCCTTTCTGCCCAGAAGAATATGTATCACCAG
ACGCAGAAGTCATAGGAGTCGAGGGACCCAAGGGAGACCCTGGCCCCCAAGGCCCACGGGGACCTGTTGG
CCCCCCTGGACAAGATGGCATCCCTGGACAGCCTGGACTTCCTGGTCCTCCTGGTCCCCCCGGCCCCCCC
GGACCCCCTGGTCTTGGAGGAAACTTTGCTTCCCAGATGTCCTATGGCTATGATGAGAAATCAGCTGGAG
TTTCCGTGCCTGGCCCCATGGGTCCTTCTGGTCCTCGTGGTCTCCCTGGCCCCCCTGGTGCACCTGGTCC
TCAAGGTTTCCAAGGCCCCCCTGGTGAACCTGGCGAGCCTGGCGCTTCAGGTCCAATGGGTCCCCGAGGT
CCCCCTGGCCCTCCTGGCAAGAACGGAGATGATGGGGAAGCTGGTAAGCCTGGCCGCCCTGGTGAGCGTG
GACCTCCTGGACCTCAGGGTGCTCGTGGATTGCCTGGAACAGCTGGCCTCCCCGGAATGAAGGGACACAG
AGGTTTCAGTGGTTTGGATGGTGCCAAAGGAGATACTGGTCCTGCTGGTCCTAAGGGAGAGCCTGGCAGT
CCTGGTGAAAATGGAGCTCCCGGCCAGATGGGTCCCCGAGGTCTGCCTGGTGAGAGAGGTCGCCCTGGAC
CCCCTGGCAGTGCTGGTGCTCGTGGTAACGATGGTGCTGTCGGTGCAGCTGGGCCCCCCGGTCCCACCGG
CCCTACTGGCCCTCCTGGCTTCCCTGGTGCAGCTGGTGCTAAGGGTGAAGCTGGTCCCCAGGGAGCCCGA
GGCTCTGAAGGTCCCCAGGGTGTGCGTGGTGAGCCCGGACCCCCTGGCCCTGCTGGTGCTGCTGGTCCTG
CTGGAAACCCTGGTGCTGATGGACAACCTGGTGCTAAAGGTGCCAATGGTGCTCCTGGTATTGCTGGTGC
TCCTGGCTTCCCTGGTGCCCGAGGCCCCTCTGGACCTCAGGGCCCCAGCGGCGCTCCTGGTCCCAAAGGC
AACAGTGGTGAACCTGGTGCCCCTGGCAACAAAGGAGACACTGGTGCCAAAGGAGAACCCGGCCCTGCTG
GAGTTCAAGGTCCCCCTGGCCCTGCTGGAGAAGAAGGAAAACGAGGAGCCCGTGGTGAGCCTGGACCTAG
CGGCCTGCCCGGACCTCCTGGCGAGCGCGGTGGACCTGGTAGCCGTGGTTTCCCTGGTGCTGATGGTGTT
GCTGGCCCCAAGGGTCCTGCTGGTGAACGTGGTTCTCCTGGCCCTGCTGGTCCCAAAGGTTCTCCTGGTG
AAGCTGGTCGCCCTGGTGAAGCTGGTCTCCCTGGTGCCAAGGGTCTCACTGGCAGTCCTGGCAGCCCTGG
TCCTGATGGCAAAACCGGCCCCCCTGGTCCCGCTGGTCAAGATGGTCGCCCTGGACCCGCAGGTCCTCCT
GGAGCCCGTGGACAGGCTGGTGTGATGGGATTCCCTGGACCTAAGGGTACTGCTGGAGAACCTGGAAAGG
CTGGAGAACGAGGTGTCCCCGGACCCCCTGGCGCTGTTGGTCCCGCTGGCAAAGATGGCGAAGCTGGAGC
TCAAGGAGCCCCCGGCCCTGCTGGTCCTGCTGGTGAGAGAGGTGAACAAGGTCCCGCTGGCTCCCCTGGA
TTCCAGGGTCTTCCTGGTCCCGCTGGTCCTCCTGGTGAAGCAGGCAAGCCTGGTGAACAGGGTGTTCCTG
GAGACCTTGGTGCCCCTGGACCCTCTGGCGCAAGAGGCGAGAGAGGTTTCCCTGGTGAACGTGGTGTACA
AGGTCCCCCAGGTCCTGCTGGTCCCCGAGGAAACAATGGTGCCCCCGGCAACGATGGTGCCAAGGGTGAT
ACTGGTGCCCCCGGAGCTCCTGGTAGCCAGGGTGCCCCCGGTCTTCAGGGAATGCCTGGTGAACGTGGTG
CAGCTGGTCTTCCAGGTCCTAAGGGTGACAGAGGTGATGCTGGTCCCAAAGGTGCTGATGGTTCTCCTGG
CAAAGATGGCGTCCGTGGTCTGACTGGTCCCATTGGTCCTCCTGGCCCTGCTGGTGCCCCTGGTGACAAG
GGTGAAACTGGTCCCAGTGGTCCTGCTGGCCCCACCGGAGCCCGTGGTGCCCCCGGAGACCGTGGTGAGC
CTGGTCCCCCTGGTCCTGCTGGCTTCGCTGGCCCCCCTGGTGCTGATGGCCAACCTGGTGCGAAAGGTGA
ACCTGGTGATACTGGTGTGAAAGGTGACGCTGGTCCTCCTGGCCCTGCTGGTCCCGCTGGACCCCCTGGC
CCCATTGGTAACGTTGGTGCTCCTGGACCCAAAGGTTCTCGTGGTGCTGCTGGTCCCCCTGGTGCTACTG
GTTTCCCTGGTGCTGCTGGTCGTGTTGGTCCCCCTGGTCCCTCTGGAAATGCTGGACCCCCTGGCCCTCC
CGGTCCCGTTGGCAAAGAAGGGGGCAAAGGTCCCCGTGGTGAGACTGGTCCCGCTGGACGTCCTGGTGAA
GTTGGTCCCCCAGGTCCCCCTGGCCCTGCTGGTGAGAAAGGATCTCCTGGTGCTGATGGACCTGCTGGCT
CTCCTGGTACCCCTGGACCTCAGGGTATTGCTGGACAGCGTGGTGTGGTCGGTCTTCCCGGTCAGAGAGG
AGAAAGAGGCTTCCCTGGTCTTCCTGGACCCTCTGGTGAACCCGGCAAACAAGGTCCTTCTGGAGCAAGT
GGTGAACGTGGTCCCCCTGGCCCTATGGGCCCCCCTGGATTGGCTGGCCCCCCTGGTGAATCTGGTCGTG
AGGGATCCCCTGGTGCTGAAGGCTCCCCTGGAAGAGATGGTGCTCCTGGTGCCAAGGGTGACCGTGGTGA
GACTGGCCCTGCTGGCCCCCCTGGTGCTCCTGGTGCTCCTGGTGCTCCCGGCCCTGTTGGTCCTGCTGGC
AAGAATGGCGACCGTGGTGAGACTGGTCCTGCTGGTCCTGCTGGTCCCATTGGCCCTGCTGGTGCCCGTG
GTCCTGCTGGACCCCAAGGCCCCCGTGGTGACAAGGGTGAGACAGGCGAACAAGGTGACAGAGGCATAAA
GGGTCATCGTGGCTTCTCTGGTCTCCAGGGTCCTCCTGGCTCTCCTGGCTCTCCTGGTGAACAAGGCCCC
TCTGGAGCTTCTGGTCCTGCAGGTCCCCGGGGTCCCCCTGGCTCTGCTGGTTCTCCTGGCAAAGATGGAC
TCAACGGTCTCCCTGGCCCCATTGGTCCCCCTGGTCCTCGAGGTCGCACTGGCGATAGTGGTCCTGCTGG
TCCCCCCGGACCTCCTGGACCCCCTGGCCCTCCCGGTCCTCCCAGCGGTGGTTATGACTTCAGCTTCCTG
CCTCAGCCACCTCAAGAGAAGTCTCAAGATGGTGGCCGTTACTACCGGGCCGATGATGCCAACGTGGTCC
GTGACCGTGACCTTGAGGTGGACACTACCCTCAAGAGCCTGAGCCAGCAGATTGAGAACATCCGCAGCCC
TGAGGGCAGCCGCAAGAACCCCGCCCGCACATGCCGTGACCTCAAGATGTGCCACTCTGACTGGAAGAGC
GGAGAGTACTGGATCGACCCTAACCAAGGCTGCAACCTGGATGCCATCAAGGTCTACTGCAACATGGAGA
CAGGTCAGACCTGTGTGTTCCCCACTCAGCCCTCTGTGCCTCAGAAGAACTGGTACATCAGCCCAAACCC
CAAGGAGAAGAAGCATGTCTGGTTTGGAGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGAAGCGAA
GGTTCCGATCCTGCCGATGTCGCTATCCAGCTGACCTTCCTGCGCCTGATGTCCACCGAGGCCTCCCAGA
ACATCACCTATCACTGCAAGAACAGCGTAGCCTACATGGACCAACAGACTGGCAACCTCAAGAAGTCCCT
GCTCCTCCAGGGCTCCAACGAGATCGAGCTCAGGGGCGAAGGCAACAGTCGATTCACCTACAGCACGCTT
GTGGATGGCTGCACGAGTCACACCGGAACTTGGGGCAAGACAGTCATCGAATACAAAACCACCAAGACCT
CCCGCCTGCCCATCATCGATGTGGCTCCCTTGGACATTGGTGCCCCAGACCAGGAATTCGGAATGGACAT
TGGCCCTGCCTGCTTCGTGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_053304
Insert Size 4362 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_053304.1, NP_445756.1
RefSeq Size 5843 bp
RefSeq ORF 4362 bp
Locus ID 29393
UniProt ID P02454
Gene Summary extracellular matrix collagen protein [RGD, Feb 2006]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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