Kdm3a (NM_175764) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Jmjd1; Jmjd1a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212495 representing NM_175764 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACAAATCTCTACTAGACAAAGCTGGCTTGGGATCCATAACTTCTGTTCGCTTCCTTGGAGATCAGC AAAGTGTATTTGTTTCCAAAGACCTTTTGAAACCTATACAGGATGTAAACAGTCTTCGGCTTTCCCTTAC TGATAATCAGACGGTCAGTAAAGAGTTTCAAGCTTTGATTGTAAAACATTTGGATGAAAGCCATCTTTTA CAAGGTGACAAGAACCTTGTTGGTTCAGAAGTAAGAATTTATAGCTTGGATCCATCTACTCAGTGGTTTT CAGCAACTGTTGTACATGGAAACCCCTCATCCAAAACTCTTCAAGTCAACTGTGAGGAGATTCCAGCGCT GAAAATTGTTGATCCAGCACTGATTCACGTTGAAGTTGTACACGATAATTTTGTAACGTGTGGTAATTCT ACAAGAATTGGAGCTGTGAAACGCAAGTCTTCTGAGAATAATGGAAGTTCAGTTTCTAAACAAGCAAAAT CTTGCTCTGAGGTCTCTCCCAGTATGTGTCCTGTACAGTCTGTTCCCACAACAGTGTGTAAGGAGATCCT GCTTGGCTGTACTGCAGCAACCCCATCTAGCAACAGACAGCAGAACACTCCCCAGGCAGCCAATTCTCCA CCTAACATTGGAGCAAAACTTCCTCAAGGATGTCACAAGCAGAGTTTACCAGAAGAAATTTCTTCCTGTC TAAATACAAAATCTGAAGTACTGAGAACAAAACCAGATGTTTGCAAAGCAGGATTACTTTCTTCAAAATC TTCTCAGGTTGGAGCTGGAGACTTGAAAATTCTGAGTGAGCCCAAAGGTAGCTGTATCCAGCCTAAAACA AACACTGATCAGGAGAGCAGACTGGAGTCTACTCCACAACCAGTCACTGGCCTTACAAAGGAGTGCTTGG TTACAAAGACTTCCTCTAAGGCAGAACTGGACAATGCCACTGCTCCTGAACTGCAGAAGCGTCTAGAGCA CACAGCTTCCACACCCGATGGTCTTTCAGACAAGCCAGAAGTGGAAGCAGGTGTCACTAGGCTTAATAGC TGTTCAGAAAAGAAGGTTGGACCTTCAGATTTAGGTTCGCAGTCACAGAATTTAAAGGAAACTTCAGTAA AAGTCGATCATGACAGCTGTTGTACAAGAAGCAGTAATAAAACCCAGACTCCCCCAGCCCGGAAGTCAGT TTTGACAGACCCAGATAAACTCAAGAAGCTACAGCAGAGTGGAGAGGCCTTTGTTCAGGATGACTCCTGT GTTAACATCGTGGCACAGCTGCCCAAGTGTCGGGAGTGTCGATTAGACAGCCTGCGCAAGGATAAGGACC AGCAGAAAGACTCGCCAGTGTTCTGTCGCTTTTTTCACTTCAGGAGGTTACAATTCAACAAACATGGTGT TCTGCGGGTAGAAGGCTTCTTAACACCAAACAAGTACGACAGTGAAGCGATTGGCTTGTGGCTGCCTTTG ACCAAAAATGTTGTGGGGACCGATTTGGACACAGCAAAGTATATCCTGGCCAATATTGGAGACCACTTCT GTCAAATGGTGATTTCTGAGAAGGAGGCTATGTCAACTATTGAGCCACACAGGCAGGTTGCTTGGAAACG AGCTGTCAAAGGAGTTAGAGAAATGTGTGATGTGTGTGACACAACCATTTTCAACCTGCACTGGGTGTGC CCTCGGTGTGGGTTTGGAGTGTGTGTAGATTGCTACCGGCTGAAGAGGAAGAATTGCCAACAGGGGGCTG CCTACAAGACTTTCTCTTGGATAAGGTGTGTGAAGAGTCAAATACATGAGCCTGAGAACCTGATGCCCAC GCAGATTATTCCTGGCAAAGCCCTCTACGATGTTGGAGACATTGTGCATTCTGTCAGAGCAAAATGGGGT ATAAAGGCCAATTGTCCCTGCTCCAACAGGCAGTTCAAGCTCTTCTCGAAGCCAGCCTTAAAGGAAGACC TGAAGCAGGCATCCTTGTCTGGAGAAAAACCAAGTCTCGGCACCATGGTACAGCAAAGCTCCCCTGTGTT GGAGCCAGCAGCCGTGTGTGGGGAAGCACCCTCCAAGCCAGCCAGCAATGTGAAGCCCATCTGTCCAGCC AACACATCCCCTTTAAACTGGCTGGCTGACCTGACCAGTGGCAATGTCAACAAGGAAAACAAGGAAAAAC AGCTGACTATGCCAATTTTAAAGAACGAAATCAAATGCCTTCCACCCTTGCCTCCTCTGAACAAGTCCAG CACTGTCCTCCATACTTTTAACAGCACAATTTTGACACCCGTGAGCAACAATAATTCTGGTTTTCTTCGA AATCTTTTGAATTCATCCACAGGAAAGACAGAAAATGGACTAAAAAACACACCCAAAATCCTTGATGATA TCTTTGCCTCTTTGGTACAAAACAAGACTTCTTCTGACTTATCCAAGAGGCCTCAAGGACTGACGATCAA GCCTAGCATTCTTGGTTTTGACACTCCTCACTACTGGCTGTGTGACAACCGCCTGCTGTGCTTGCAAGAC CCCAACAATAAGAGCAATTGGAATGTCTTTAGGGAATGCTGGAAACAGGGGCAGCCAGTGATGGTGTCGG GAGTGCATCATAAATTAAACACTGAACTCTGGAAACCCGAGTCCTTCAGGAAAGAGTTTGGCGAGCAAGA AGTAGACCTAGTCAATTGTAGGACCAATGAAATCATCACAGGAGCCACAGTGGGAGACTTCTGGGATGGA TTTGAAGATGTTCCAAACCGTTTGAAAAACGAAAAAGAAAAAGAACCAATGGTGTTGAAACTTAAGGACT GGCCGCCAGGAGAAGACTTTAGAGATATGATGCCTTCCAGGTTTGATGATCTGATGGCCAACATTCCACT GCCTGAGTATACCAGGCGAGATGGCAAACTGAACCTGGCCTCTAGACTGCCAAACTACTTTGTACGGCCA GACCTGGGCCCCAAGATGTACAATGCTTATGGATTGATCACTCCAGAAGATCGGAAATATGGGACCACGA ATCTTCACTTAGATGTATCTGATGCAGCCAATGTCATGGTTTATGTGGGAATTCCCAAAGGACAGTGTGA ACAAGAGGAAGAAGTCCTTAGAACCATCCAAGATGGAGATTCGGATGAACTCACAATCAAGCGATTTATT GAAGGAAAAGAGAAGCCAGGCGCCCTTTGGCACATATATGCTGCTAAAGACACAGAAAAGATAAGAGAAT TCCTTAAAAAGGTATCTGAAGAGCAGGGTCAAGAAAACCCCGCAGACCATGATCCTATTCATGATCAGAG CTGGTATTTAGACCGATCACTGAGAAAGCGCCTTTATCAAGAGTATGGAGTTCAAGGCTGGGCTATTGTA CAATTTCTTGGCGATGTGGTGTTTATCCCGGCAGGAGCTCCACATCAGGTTCATAACTTATACAGCTGTA TCAAAGTCGCTGAAGACTTCGTGTCTCCAGAGCATGTGAAACACTGCTTCTGGCTCACTCAGGAATTCCG TCACTTGTCACAGACTCATACCAACCACGAAGATAAACTGCAGGTAAAGAATGTTATCTACCATGCAGTA AAAGATGCAGTTGCTATGCTGAAAGCAGTGAATCCAGTTTGGGCAAATGTTAACTCCTCTGCACATTGGA GATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_175764
Insert Size	3645 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_175764.2, NP_786940.1
RefSeq Size	4505 bp
RefSeq ORF	3645 bp
Locus ID	312440
UniProt ID	Q63679
Gene Summary	may play a role in spermatogenesis [RGD, Feb 2006]

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