Piezo1 (NM_001077200) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Fam38a; Mib
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN212770 representing NM_001077200 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGCACGTGCTGGGCGCCGGGCTCTACTGGCTGTTGCTACCCTGCACACTCCTGGCGGCCTCCC TGTTACGCTTCAATGCTCTCTCGTTGGTCTATTTGCTGTTTCTACTGCTGCTACCCTGGCTTCCAGGCCC CTCAAGACACAGCATACCAGGTCACACAGGTCGCCTGCTTCGTGCACTACTCTGCCTCAGCCTCCTCTTC CTGGTGGCCCATGTTGCCTTCCAGATATGCCTACATACCATGCCTCGCCTGAACCAGCTTCTGGGACAAA ACTGTAACCTTTGGGCAAACGTGTCTCAACACATAGGGGTTACAAGGCTGGACCTGAAGGACATCTTTAA CACCACCAGACTGGTGGCACCTGACCTGGGAGTGCTGGTGGCATCTTCCCTTTGCCTTGGCCTCTGTGGA CGCCTCACAAGGAAAGCCCGGCAGAGCCAGCGCACTCAGGAGCTGGAGGAGGATGACATAGATGCTGCCC CCGCCGCAGGGCTGCAGGGAGCCCCCACCCTGGCAACCAAACGCAGGCTGTGGCTGGCCCCTCGCTTCCG GATCACGGCGCACTGGCTGCTGGTGACCTCCGGACGGATGCTGGTCATCGTGCTACTGGCACTCGCAGGC ATAGCCCATCCTTCGGCCTTCTCCAGCGTCTACCTGATGGTGTTCCTGGCCATCTGCACCTGGTGGTCTT GCCACTTTCCTCTCAGCTCCCTGGGCTTTAACACCCTCTGCGTCATGGTGAGCTGCTTTGGCGCCGGCCA TCTTGTTTGTTTATACTGCTATCAGACACCATTTGTCCAGAGCGTGCTACTTCCTGGCAGCCTCTGGGCC AGGCTATTTGGTCTCAAGAACTTCGTAGACATCCCTAACTGCTCCAGCCCCAACGTGCTGGTACTCAACA CTAAGCATGCTTGGCCCATCTACGTGAGCCCCGGTATCCTGCTGCTGTTGTACTACACAGCCACCTCTCT CCTGAAGCTCCGCAAGGGCCGCTTCTCAGAGCTGAGGAAGGAGATCCCGAGGGAGGATGAGGAGCATGAA CTGGAACTGGACCAGCTGGAGCCAGAGCCCCAGGCTAGGGGCACCACCCAGGGTGCGACGCCCACGACCA CTGGACCCGACATTGACAACTGCACCGTGCATGTACTAACCAGCCAGAGCCCCGTCCGCCAGCGTCCTGT ACGCCCCAGGCTGGCTGAGCTGAAAGAGATGTCACCACTACATGGCCTGGGCCACCTCATCCTGGACCAG AGCTATGTGTGTGCTCTGATCGCCATGATGGTGTGGAGCATCATGTACCACAGCTGGCTGACCTTCGTCT TGCTGCTCTGGGCCTGCCTCATCTGGACCGTGCGCAGCCGCCACCAGTTGGCTATGCTGTGCTCACCCTG CATCCTGCTGTACGGACTGACACTCTGCTGCCTGCGGTACGTGTGGGCCATGGAGCTCCCCGAGCTGCCC ACCACCCTGGGCCCTGTCAGTCTACATCAGTTGGGATTGGAGCATACACGCTACCCCTGCCTGGACCTTG GTGCCATGCTGCTGTATCTGCTCACGTTCTGGCTGCTCCTGCGTCAGTTCGTGAAGGAGAAGCTGCTGAA GAAGAGAAAGGCACCTTCGACGCTGCTGGAGGTCACAGTGTCCGACACAGAGCCCACACAGACCCAGACA CTGCTGAGGAGCCTGGGGGAGCTGGTCACGGGCATCTACGTCAAGTACTGGATCTACGTGTGCGCTGGGA TGTTCATTGTGGTCAGCTTCGCTGGCCGCCTGGTGGTCTACAAAATCGTCTACATGTTCCTTTTCCTGCT GTGCCTCACGTTGTTCCAGGTCTACTACACTCTGTGGAGGAAGCTGCTCCGAGTTTTCTGGTGGCTCGTG GTGGCCTACACCATGCTCGTGCTCATCGCCGTGTACACTTTCCAGTTCCAGGACTTCCCCACCTACTGGC GCAACCTCACGGGCTTCACAGATGAACAGTTGGGCGACCTGGGCCTGGAGCAGTTCAGCGTGTCGGAGCT CTTTTCCAGCATCCTCATCCCTGGCTTCTTCCTGCTGGCCTGCATCCTGCAGCTGCACTACTTCCACAGG CCGTTCATGCAGCTCACCGACCTGGAGCACGTGCCCCCGCCAGGCACACGCCGCCTTCGCTGGGCTCACA GGCAGGATACAGTGAGCGAGGCCCCTCTGCTTCAGCATCAGGAGGAGGAGGAAGTCTTCAGGGATGACGG GCAGAGCATGGATGGGCCCCACCAGACCACACAGGTCCCTGAGGGTACAGCCAGCAAGTGGGGCCTGGTG GCCGACCGGCTGCTGGACCTAGCGTCCAGCTTCTCCGCCGTCCTCACCCGCATCCAGGTGTTCGTACGAT GTTTGCTCGAGCTTCACGTCTTCAAGCTGGTGGCTCTCTACACTGTCTGGGTGGCCCTGAAAGAAGTGTC CGTGATGAACCTGCTGCTGGTGGTGCTGTGGGCCTTCGCCCTGCCCTACCCGCGCTTCCGGCCCATGGCC TCCTGCCTGTCCACCGTGTGGACCTGTATCATCATCGTGTGCAAGATGCTGTATCAGCTGAAGATTGTCA ACCCACACGAGTACTCCAGTAACTGCACCGAGCCCTTTCCCAACAATACCAACTTGCAGCCCTTGGAGAT CAGCCAGTCTCTGCTGTACCGTGGCCCCGTTGACCCTGCCAACTGGTTTGGGGTGCGGAAGGGTTACCCA AACTTGGGCTATATCCAGAACCACCTGCAGATCCTTCTGCTGCTGGTGTTTGAGGCTGTGGTGTACCGGC GCCAAGAGCACTACCGCCGGCAGCACCAGCAGGCCCCTCTGCCCGCCCAGGCTTTGTGCGCTGATGGCAC CCGCCAGAGGCTGGACCAGGACCTACTCAGCTGCCTCAAATATTTCATCAACTTCTTCTTCTACAAATTC GGGCTGGAGATCTGCTTCTTGATGGCCGTGAATGTCATCGGACAACGCATGAACTTCATGGTGATCTTAC ACGGCTGCTGGTTGGTGGCCATCCTTACACGCCGGCGCCGTGAGGCCATCGCCCGCCTCTGGCCCAATTA CTGTCTGTTCCTCACGCTGTTCCTGCTGTACCAGTACCTGCTGTGCCTGGGCATGCCTCCCGCACTGTGC ATCGACTACCCGTGGCGCTGGAGCCAGGCCATCCCCATGAATTCTGCGCTCATCAAGTGGCTGTACCTCC CTGACTTCTTCAGGGCCCCCAACTCCACCAACCTTATCAGTGACTTCCTCCTGCTACTCTGCGCCTCCCA ACAGTGGCAGGTGTTCTCCGCAGAGCAAACGGAAGAATGGCAGCGCATGGCTGGCGTTAACACTGACCAC CTGGAGCCCCTGCGTGGGGAGCCCAACCCTATCCCCAACTTCATCCACTGCAGGTCCTATCTGGATATGC TGAAGGTGGCCGTCTTCCGCTACCTGTTCTGGCTGGTGCTGGTGGTCGTGTTTGTCACAGGGGCCACCCG CATCAGCATCTTTGGGCTGGGGTACCTGCTGGCCTGCTTCTACCTGCTGCTCTTTGGCACCACCCTGCTG CAGAAGGACACACGAGCCCAGCTCGTGCTGTGGGACTGCCTCATCCTCTACAACGTCACCGTCATCATCT CTAAGAATATGTTGTCGCTCCTGTCCTGTGTCTTCGTGGAGCAAATGCAGAGCAACTTCTGCTGGGTCAT CCAGCTCTTCAGCCTCGTGTGCACAGTCAAAGGCTACTATGACCCCAAAGAGATGAAGACCAGGGACCGG GATTGCCTGCTGCCTGTGGAGGAGGCCGGGATCATCTGGGACAGCATCTGCTTCTTCTTCCTGCTCCTAC AACGGCGCGTCTTTCTCAGCCACTACTTCCTGCACGTCAGTGCTGACCTGAAGGCCACGGCCCTGCAGGC CTCCAGGGGTTTTGCTCTCTACAATGCGGCCAACATAAAGAACATCAACTTCCACCGTCAGACCGAGGAG AGGTCCCTGGCCCAGCTGAAAAGACAGATGAAGCGCATCCGTGCCAAACAGGAGAAATACCGGCAGAGCC AGGCAAGCCGTGGTCAACTCCAGTCCACAGACCCTCAGGAGCCAGGGCCTGACAGCCCAGGGGGTTCCTC CCCGCCACGGACACAGTGGTGGCGCCCCTGGCAGGACCACGCCACAGTCATCCACTCTGGTGACTACTTC CTGTTTGAGTCGGACAGTGAGGAGGAAGAGGAGGCCCTGCCTGAGGACCCCAGGCCTGCAGCTCAGAGTG CCTTCCAGATGGCATACCAGGCATGGGTAACCAACGCCCAGACAGTGCTAAGGCAGCGTCGTGAACAGGC ACGGCGGGATCGGGCAGAACAGCTGGCTTCTGGAGGCGACTTGAGTCCTGAGGTGGAGCTGGTAGACGTC CCAGAGAATGAGATGGCAGGCCATAGCCATGTGATGCAGCGCGTGCTGAGTACCATGCAGTTCCTGTGGG TGCTGGGCCAGGCCACAGTGGACGGGCTGACGCGCTGGTTGCGTACGTTCACGAAGGACCACCGCACCAT GAGTGATGTGCTGTGTGCAGAGCGTTACCTGCTCACCCAGGAGCTTCTTCGGGGTGGAGAGGTACACCGA GGGGTACTGGACCAGCTTTACGTGAGCGAAGATGAGATCGCACTGTCAGGTCCTGTGGAGAACCGTGATG GACCCAGCACAGCCTCTAGCGGGCTGGGAGCTGAAGAGCCTTTGAGTAGCATGACAGACGACACCGGCAG CCCCCTTAGCACTGGCTACAACACTCGCAGTGGTAGTGAGGAGATTATCACCGACACTGGGGGCCTCCAG GCTGGGACCTCCCTGCACGGCTCCCAAGAGCTTCTAGCCAATGCTCGTACCCGGATGCGCACAGCCAGTG AGCTGCTGCTGGACAGGCGCCTGCGTATCCCTGAGCTGGAGGAGGCCGAGCAGTTTGAGGCACAGCAGGG CCGGACGCTGCGGCTGCTCAGAGCCATGTACCAGTGTGTGGCGGCCCACTCAGAGCTTCTCTGTTACTTC ATCATCATCCTTAACCACATGGTGACAGCCTCGGCTGCCTCCCTGGTGCTGCCTGTGCTTGTGTTCCTCT GGGCCATGCTGACCATCCCGAGGCCTAGCAAGCGCTTCTGGATGACAGCTATCGTCTTCACTGAGGTCAT GGTGGTCACCAAATACTTGTTCCAGTTTGGCTTCTTCCCCTGGAACAGCTACATAGTGCTTCGACGCTAC GAGAACAAGCCCTACTTCCCTCCGCGCATCCTGGGCCTCGAGAAAACAGACAGCTATATCAAGTACGACC TGGTGCAACTCATGGCCCTCTTCTTCCACCGCTCGCAGTTACTGTGCTATGGCCTCTGGGACCATGAGGA GGATGGTGTCCCCAAGGACCATTGCAGGAGTAGCGAAAAGGACCAGGAAGCTGAGGAAGAGTCAGAGGCT AAGCTGGAATCGCAGCCTGAGACGGGCACTGGGCATCCTGAGGAGCCCGTGTTGACTGGTACCCCCAAGG ACCACATCCAAGGGAAAGGAAGTGTTAGGTCCAAGGATGAGATCCAAGATCCCCCAGAGGACCTTAAGCC CCAGCACAGGAGACACATCAGCATACGCTTCAGGAGGAGGAAGGAGACTCAAGGACCCAAAGGAGCAGCA GTCGTGGAGGCTGAGCACGAGGAGGGAGAGGAAGGAAGAGAAGCTGCAGGGAGAAAGAGGCTGCGTCGCC CTCGAGAAGGGTTGAAGATTCGGGAGAAAATGAAGGCAGCTGGACGCCGGCTGCAGAGCTTCTGTCTGTC ACTGGCCCAGAGCTTTTACCAACCCCTGCGGCGTTTCTTCCATGACATTCTGCACACCAAGTACCGAGCG GCCACCGACGTCTATGCCCTCATGTTCCTGGCTGACATTGTCGACATTGTCGTCATCATCTTCGGTTTTT GGGCTTTTGGGAAGCACTCCGCGGCCACGGACATTGCATCCTCACTGTCAGATGACCAGGTGCCACAGGC CTTCCTGTTCATGCTGCTGGTCCAGTTCGGCACCATGGTCATAGACCGCGCCCTCTACCTGCGTAAGACT GTACTGGGCAACCTGGCCTTTCAGGTGGTCCTGGTGGTGGCCATTCACCTCTGGATGTTTTTCATCTTAC CGGCTGTCACCGAGAGGATGTTCAGACAGAACGCGGTGGCCCAGCTGTGGTACTTCGTGAAGTGCATTTA CTTTGCCCTGTCCGCCTACCAGATCCGCTGTGGCTACCCCACCCGTATCTTGGGCAACTTCCTCACCAAG AAGTACAATCATCTAAACCTCTTCCTTTTCCAGGGGTTCCGTCTAGTGCCATTCCTGGTGGAGCTGCGGG CCGTCATGGACTGGGTATGGACTGACACCACGCTGTCCCTGTCCAACTGGATGTGTGTGGAAGACATCTA TGCCAACATCTTTATCATCAAGTGCAGCCGAGAGACAGAGAAGAAATACCCCCAGCCCAAGGGACAGAAG AAGAAGAAAATCGTCAAGTATGGCATGGGAGGTCTCATCATCCTCTTCCTCATTGCCATCATCTGGTTCC CTCTGCTCTTCATGTCACTGATCCGCTCTGTGGTTGGTGTCGTCAACCAGCCCATTGATGTCACTGTTAC CCTCAAGTTAGGTGGCTACGAGCCACTGTTCACCATGAGCGCCCAGCAGCCATCCATAGTGCCATTCACT CCCGAGGACTATGAGGAGCTGTCCCAGCAGTTTGACCCCTACCCACTAGCCATGCAGTTCATTAGCCAGT ACAGTCCTGAGGACATCGTCACTGCACAGATTGAGGGCAGCTCGGGGGCCCTGTGGCGCATCAGCCCGCC CAGCCGGGCCCAGATGAAGCACGAGCTGTACAACGGCACAGCCGACATCACACTGCGCTTCACCTGGAAT TTCCAAAGGGACCTGGCCAAGGGCGGCTCTGTGGAGTATACCAACGAGAAGCACACCTTGGAGCTGGCCC CCAACAGTACGGCACGAAGGCAGCTGGCCCAACTGCTCGAGGGCAGACCTGACCAGTCAGTGGTCATTCC CCACCTCTTCCCCAAGTACATTCGTGCACCCAATGGGCCTGAAGCCAATCCTGTGAAGCAACTGCAGCCA GACGAGGAAGAGGACTACCTTGGTGTGCGTATCCAGCTGCGGAGAGAGCAAGTGGGCACAGGAACCTCTG GGGAGCAAGCAGGCACAAAGGCCTCCGACTTCCTCGAGTGGTGGGTGATTGAGCTGCAGGACTGCCAGGC CGAGTGCAACCTGCTGCCCATGGTCATCTTCAGTGACAAGGTCAGCCCGCCTAGCCTGGGCTTCCTGGCC GGCTACGGGATTGTGGGGCTCTACGTCTCTATCGTGCTGGTCGTTGGCAAGTTTGTGCGGGGCTTCTTCA GTGATATCTCACACTCCATCATGTTCGAGGAGCTGCCGTGCGTGGACCGCATCCTCAAGCTGTGCCAGGA CATCTTCTTGGTACGGGAGACCCGCGAGCTGGAGCTGGAGGAGGAACTCTATGCCAAGCTCATATTCCTG TACCGATCACCAGAGACCATGATCAAGTGGACGCGTGAGAAGGAGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001077200
Insert Size	7608 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001077200.2, NP_001070668.2
RefSeq Size	8221 bp
RefSeq ORF	7608 bp
Locus ID	361430
UniProt ID	Q0KL00
Gene Summary	Pore-forming subunit of a mechanosensitive non-specific cation channel. Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation. Plays a key role in blood vessel formation and vascular structure in both development and adult physiology.[UniProtKB/Swiss-Prot Function]

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