Pycr3 (NM_001011993) Rat Untagged Clone

CAT#: RN212963

Pycrl (untagged ORF) - Rat pyrroline-5-carboxylate reductase-like (Pycrl), (10 ug)



  "NM_001011993" in other vectors (3)

CNY 3,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Rat Untagged Clone
Tag Tag Free
Synonyms Pycrl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RN212963 representing NM_001011993
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGATGAGATGTCTGAACCTCGGCGTGTGGGCTTCGTAGGTGCTGGTCGCATGGCGGAAGCCATTG
CCCAAGGCCTCATCCGAGCAGGCAAAGTAGAAGCAAAGCAAGTGCTGGCCAGTGCACCAACAGACAAGAA
CCTCTGCCACTTCAGGGCTCTGGGTTGCCAGACTACTCACTCCAACCACGAGGTGCTGCAAAACTGCCCA
CTCGTCATCTTTGCCACCAAACCCCAAGTCCTGCCAGCTGTTCTGGCAGAAGTGGCCCCTGTAGTCACCA
CTGAGCACATCATTGTATCCGTGGCTGCTGGGATCTCTCTGAGCTCTATGGAGGAGCTGTTACCCCCGAA
AACACGAGTACTGCGAGTCTCTCCCAATCTACCCTGCGTAGTTCAGGAGGGAGCTATGGTTATGACACGG
GGCCACCATGCTGGGAATGAAGACGCCAAGCTCCTACAGAACTTGCTGGAAGCCTGTGGGCAGTGCATAG
AGGTTCCTGAGTCCTACGTCGATATCCACACTGGCCTCAGCGGCAGTGGTGTGGCCTTTGTGTGTACATT
TTCGGAGGCCCTGGCTGAAGGCGCCATCAAGATGGGCATGCCCAGTGACCTGGCCCACCGCATTGCCGCT
CAGACCCTGCTGGGGACAGCCAAAATGCTGCAGCAGGAAGGGAAGCACCCAGCCCAGCTGCGGACGGATG
TGCTCACACCAGCTGGAACCACCATCCACGGGCTTCATGCCCTAGAGCAGGGCGGTTTTCGAGCAGCTGC
CATGAGTGCTGTGGAAGCAGCTACCTGCCGGGCTAAAGAGCTCAGCAAGAAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001011993
Insert Size 825 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001011993.1, NP_001011993.1
RefSeq Size 1489 bp
RefSeq ORF 825 bp
Locus ID 300035
UniProt ID Q5PQJ6
Gene Summary Enzyme that catalyzes the last step in proline biosynthesis. Proline is synthesized from either glutamate or ornithine; both are converted to pyrroline-5-carboxylate (P5C), and then to proline via pyrroline-5-carboxylate reductases (PYCRs). PYCRL is exclusively linked to the conversion of ornithine to proline.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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