Sf3b1 (NM_053426) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Sap155
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN213073 representing NM_053426 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAAGATCGCCAAGACTCACGAAGATATCGAAGCACAGATTCGAGAAATTCAAGGCAAGAAGGCAG CTCTTGATGAAGCCCAAGGAGTGGGCCTTGATTCCACAGGTTATTATGACCAAGAAATTTATGGTGGAAG TGATAGCAGGTTTGCTGGATATGTGACATCAATTGCTGCGACTGAACTTGAAGATGATGACGATGACTAC TCATCATCCACTAGCTTGCTTGGTCAGAAGAAGCCTGGATATCATGCCCCCGTGGCATTGCTTAATGATA TACCACAGTCAACAGAACAGTATGATCCATTCGCTGAGCATCGTCCTCCAAAGATTGCAGACCGGGAAGA TGAATATAAAAAGCATAGGCGGACCATGATAATTTCCCCAGAGCGTCTTGATCCTTTTGCAGATGGAGGG AAGACCCCTGATCCTAAAATGAATGCTAGAACCTACATGGATGTTATGCGAGAACAACATTTGACTAAGG AAGAGAGAGAAATTAGGCAACAACTAGCAGAAAAAGCTAAAGCTGGAGAACTAAAAGTTGTCAACGGAGC AGCAGCATCCCAGCCTCCCTCAAAACGAAAACGGCGCTGGGATCAAACTGCTGACCAGACTCCTGGTGCC ACTCCCAAAAAGCTGTCAAGTTGGGATCAGGCAGAGACCCCTGGGCATACCCCTTCTTTAAGATGGGATG AGACACCGGGTCGTGCAAAAGGAAGTGAAACACCTGGAGCAACCCCAGGCTCAAAAATATGGGATCCTAC ACCTAGTCATACACCTGCGGGAGCTGCTACTCCTGGACGAGGAGATACACCAGGCCATGCAACCCCGGGC CATGGCGGTGCAACTTCCAGTGCCCGTAAAAACAGATGGGATGAGACCCCCAAAACAGAAAGAGATACTC CTGGGCATGGAAGTGGGTGGGCTGAGACTCCTCGAACAGACCGAGGTGGAGACTCTATTGGTGAGACACC AACTCCTGGAGCAAGTAAAAGAAAGTCTCGTTGGGACGAAACACCAGCTAGTCAAATGGGCGGAAGCACT CCTGTTCTGACTCCAGGAAAAACACCAATTGGCACACCAGCCATGAACATGGCCACCCCCACTCCAGGTC ACATAATGAGCATGACTCCTGAGCAGCTTCAGGCTTGGCGGTGGGAGAGAGAGATTGATGAGCGCAACCG CCCACTTTCTGATGAGGAATTAGATGCTATGTTCCCAGAAGGATACAAGGTCCTTCCCCCTCCAGCTGGC TATGTTCCTATTCGAACTCCAGCTCGAAAGCTGACAGCAACTCCCACACCTTTGGGCGGTATGACTGGTT TTCACATGCAAACTGAAGACAGAACTATGAAAAGTGTCAACGACCAGCCATCTGGGAATCTTCCATTTCT AAAACCTGATGACATTCAGTACTTTGACAAACTATTGGTTGATGTAGATGAGTCAACACTTAGTCCAGAA GAGCAAAAAGAAAGAAAAATAATGAAGTTGCTTTTAAAAATTAAGAATGGTACACCTCCAATGAGAAAGG CTGCCTTACGTCAGATTACTGATAAAGCTCGAGAATTTGGAGCTGGACCACTGTTTAACCAGATTCTGCC TCTATTGATGTCCCCTACACTTGAGGATCAAGAGCGACATTTACTTGTGAAAGTTATTGACAGAATATTG TATAAACTTGATGACTTGGTGCGGCCATATGTGCACAAGATTCTTGTGGTTATCGAGCCACTGTTGATTG ATGAAGATTACTATGCTAGAGTGGAAGGCCGAGAAATTATTTCTAATTTGGCAAAGGCTGCTGGTCTGGC TACTATGATTTCTACCATGAGACCTGATATAGATAACATGGATGAATATGTCCGAAACACAACAGCTAGA GCTTTTGCTGTTGTAGCCTCTGCTCTGGGCATTCCTTCTTTATTGCCTTTCTTGAAAGCTGTGTGTAAAA GCAAGAAGTCTTGGCAAGCGAGGCACACTGGTATTAAGATTGTACAACAGATAGCCATTCTCATGGGCTG TGCTATCTTGCCACATCTCAGAAGTTTAGTTGAAATCATTGAGCATGGTCTCGTGGATGAGCAGCAGAAA GTTCGGACCATCAGTGCTCTGGCCATTGCTGCTTTGGCTGAGGCAGCAACTCCTTATGGTATTGAGTCTT TTGATTCTGTGCTCAAGCCCCTATGGAAGGGTATTCGCCAACATAGAGGAAAGGGTCTGGCTGCTTTCTT GAAGGCTATTGGGTATCTTATTCCTCTTATGGATGCAGAATATGCCAACTACTATACTAGAGAAGTGATG CTAATCCTTATTCGAGAATTCCAGTCTCCTGATGAAGAAATGAAGAAAATTGTTTTGAAGGTGGTGAAAC AGTGTTGTGGAACTGATGGTGTAGAGGCAAACTACATTAAGACAGAGATTCTTCCACCTTTCTTTAAACA CTTCTGGCAGCACAGAATGGCTTTGGATAGAAGAAATTACCGACAGTTAGTCGATACAACTGTGGAGTTG GCAAACAAAGTGGGCGCGGCAGAGATCATATCTAGGATTGTTGACGATCTGAAGGATGAGGCTGAGCAGT ACCGGAAAATGGTGATGGAAACCATTGAGAAAATTATGGGCAATCTAGGTGCAGCGGATATTGACCATAA ACTTGAGGAACAGCTTATCGATGGCATTCTCTATGCTTTCCAAGAACAGACTACGGAGGATTCAGTAATG CTGAATGGCTTTGGCACAGTAGTCAATGCTCTTGGCAAACGAGTCAAACCATACTTGCCTCAGATCTGTG GTACAGTTTTATGGCGTTTGAATAATAAATCAGCAAAAGTTAGGCAACAAGCAGCTGACTTGATTTCTCG AACTGCTGTTGTCATGAAGACTTGTCAAGAGGAAAAACTGATGGGGCACTTGGGTGTGGTGCTGTATGAG TATCTGGGTGAAGAGTACCCTGAAGTATTGGGCAGCATTCTTGGAGCACTGAAGGCTATTGTAAATGTAA TAGGTATGCATAAGATGACACCACCAATTAAAGATCTGTTGCCTAGACTCACCCCCATCTTAAAGAACAG ACATGAAAAAGTACAAGAGAATTGTATTGATCTTGTTGGGCGTATTGCTGACAGGGGAGCTGAGTATGTA TCTGCAAGAGAGTGGATGAGGATTTGCTTTGAACTTTTAGAGCTTTTAAAAGCTCACAAAAAAGCTATTC GAAGAGCTACAGTCAATACATTTGGTTATATCGCAAAGGCTATTGGCCCTCACGATGTATTGGCTACACT TCTAAACAACCTCAAAGTTCAGGAAAGGCAGAACAGGGTTTGTACGACTGTAGCAATAGCCATTGTTGCT GAAACATGCTCACCCTTCACAGTACTCCCTGCCCTGATGAATGAGTACAGAGTTCCTGAGCTTAATGTTC AAAATGGAGTGTTAAAATCACTCTCCTTCCTGTTTGAGTATATCGGTGAAATGGGAAAAGACTACATCTA CGCTGTAACACCTTTACTTGAGGATGCATTAATGGATAGGGACCTTGTACACAGACAGACAGCTAGTGCA GTGGTACAACACATGTCACTTGGTGTTTATGGATTTGGCTGTGAAGATTCTCTGAATCACTTACTGAACT ATGTGTGGCCTAATGTGTTTGAGACATCTCCCCATGTAATCCAGGCAGTTATGGGAGCTCTGGAGGGCCT GAGAGTTGCTATTGGACCATGCAGAATGTTGCAGTATTGCTTACAGGGCCTGTTTCACCCAGCTCGGAAA GTCAGAGATGTATATTGGAAAATTTACAACTCCATCTACATTGGCTCCCAGGATGCTCTCATAGCACATT ACCCAAGGATCTACAATGATGATAAGAACACCTATATTCGTTACGAACTTGACTATATCTTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053426
Insert Size	3915 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053426.1, NP_445878.1
RefSeq Size	4954 bp
RefSeq ORF	3915 bp
Locus ID	84486
Gene Summary	mouse homolog is a subunit of the U2 snRNP that is necessary for prespliceosome assembly and splicing catalysis [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.